Research on the Effect of Microbial Fermentation Feeds on Slaughter Performance and Meat Quality in Pig with Large-scale Feeding

In this paper, Duroc-lanndrae-Yorkshire crossbred piggy of born at 75 days of age, bouncing with health and similar body weight were randomly divided into experimental group （136 pigs） and control group （136 pigs）. Pigs in control group were fed with conventional diets, and 4% microbial fermentation feeds were added to the basal diet of experimental group to research on the effect of performance and meat quality of pig breeding. The results indicated that after feeding 106 d, compared to control group, the slaughter performance, meat quality and content of fatty acid and amino acid of experimental group were all better, its slaughter rate increased 1.53%, the carcass weight increased 2.75% and the backfat thickness decreased 0.02 cm. In addition, the pork meat in test group hadn＇t antibiotic and heavy metal residues, while it had higher color score, lower pH45min and pH24h value, better tenderness and rate of cooked meat and higher content of fatty acids, polyunsaturated fatty acids and amino acids. Microbial fermentation feed using in test group can significantly improved the flavor and aroma of pork, and eating this pork conducive more to human health. Thus, the application of microbial fermenta- tion feed can provide a more safe, healthy and nutritious flavor pork for human beings.

灵芝液态深层发酵产物中10种不饱和脂肪酸类化合物的鉴定

通过规模化液态深层发酵获得灵芝发酵产物,采用多种硅胶色谱柱层析及重结晶的方式,从中分离得到10个化合物。通过核磁、质谱等波谱分析,鉴定出这些化合物均属于含羟基或酮基的不饱和脂肪酸类化合物,分别为(9S,10R,11E,13R)-9,10,13-trihydroxyoctadec-11-enoicacid(1)和(9S,10R,11E,13S)-9,10,13-trihydroxyoctadec-11-enoicacid(2)的混合物、12S^(*),13S^(*)-dihydroxy-9-oxo-10(E)-octadecenoicacid(3)、9R^(*),10R^(*)-dihydroxy-13-oxo-11(E)-octadecenoicacid(4)、12S^(*),13R^(*)-dihydroxy-9-oxo-10(E)-octadecenoicacid(5)、9S^(*),10R^(*)-dihydroxy-13-oxo-11(E)-octadecenoicacid(6)、10(S)-hydroxy-8(Z)-octadecenoic acid(7)、12-oxooctadeca-8,10-dienoic acid(8)、9,12-dihydroxy-10-eicosenoic acid(9)和9-oxooctadeca-10,12-dienoicacid(10)。这些化合物均为首次从灵芝发酵产物中获得,且具有不同程度的体外抗肿瘤活性。其中,化合物8和化合物10对L1210细胞增殖抑制的IC_(50)值分别为13.00μmol/L和16.88μmol/L,对K562细胞增殖亦有良好的抑制效果,是具有抗肿瘤潜力的天然产物。

贝莱斯芽孢杆菌B6强化驱油机制分析及在高矿化度油藏中的应用

【目的】筛选油藏内源功能微生物并探究其驱油机理,现场试验以确定油藏内源微生物提高原油采收技术应用的工艺和技术可行性。【方法】采集英东油田样品,利用原油平板筛选驱油功能菌,评价其环境适应性并优化培养条件,通过乳化性能、降黏防蜡和烃转化能力等评估其驱油性能并探索其潜在机制,并在油田现场进行微生物强化驱油试验。【结果】从油水样品中分离到的贝莱斯芽孢杆菌(Bacillus velezensis)B6,其乳化活性指数(emulsifying activity index,EI24)值为100.00%,降黏率为97.20%,防蜡率为86.90%,表明菌株B6具有良好的乳化降黏性能,具有提高原油采收的潜力,并且能够减少原油中的重质组分而增加轻质烃类,改善原油物性,提升原油品质。进一步在英东油田、跃进油田和花土沟油田进行微生物单井吞吐和清防蜡作业,共进行62井次现场试验,累计增油1460.36 t,平均延长洗井周期47 d,经济效益为342.50万元,投入产出比为1:4。【结论】通过室内研究和现场试验,证实内源微生物Bacillus velezensis B6具有显著提高原油采收和油井清防蜡的效果,应用潜力巨大。