Objective: To study immunologic alterations in patients with neuromyelitis opt ica (NMO). Methods: The authors studied 8 patients with NMO together with 16 hea lthy subjects, 16 patients with relapsing remitting multi...Objective: To study immunologic alterations in patients with neuromyelitis opt ica (NMO). Methods: The authors studied 8 patients with NMO together with 16 hea lthy subjects, 16 patients with relapsing remitting multiple sclerosis (RRMS), a nd16 patients with secondary progressive MS (SPMS), matched for age and sex, as controls. Because recent histopathologic studies have demonstrated that active N MO lesions consist of perivascular immunoglobulin (Ig) deposition and eosinophil in filtration, IL-5, IL-6, IL-12, IgG, and IgM production by anti-myelin oli godendrocyte glycoprotein (MOG) mononuclear cells in peripheral blood and CSF we re selected for study using ELISPOT. Eotaxin-2 (Eo-2) and eotaxin-3 (Eo-3)le vels were also assessed using ELISA and eosinophil cationic protein (ECP) levels by radioimmunoassay. Results: MOG specific responses in CSF showed significant increase in IL-5,IL-6, IgG, and IgM secreting cells in NMO patients compared w ith patients with RRMS, SPMS and healthy subjects. Interestingly,numbers of IgM secreting cells were significantly higher than identical specificity IgG secreti ng ones. Moreover,CSF Eo-2, Eo-3, and ECP levels were also significantly highe r in NMO patients compared to all three control populations.Anti-MOG IL-12 sec reting cells were increased in CSF and peripheral blood from NMO, RRMS, and SPMS patients when compared to healthy subjects. Conclusions: These observations suggest that neu romyelitis optica is associated with amajor humoral immune response (particularl y anti-MOG IgM production) and eosinophil activation present exclusively in CSF .展开更多
文摘Objective: To study immunologic alterations in patients with neuromyelitis opt ica (NMO). Methods: The authors studied 8 patients with NMO together with 16 hea lthy subjects, 16 patients with relapsing remitting multiple sclerosis (RRMS), a nd16 patients with secondary progressive MS (SPMS), matched for age and sex, as controls. Because recent histopathologic studies have demonstrated that active N MO lesions consist of perivascular immunoglobulin (Ig) deposition and eosinophil in filtration, IL-5, IL-6, IL-12, IgG, and IgM production by anti-myelin oli godendrocyte glycoprotein (MOG) mononuclear cells in peripheral blood and CSF we re selected for study using ELISPOT. Eotaxin-2 (Eo-2) and eotaxin-3 (Eo-3)le vels were also assessed using ELISA and eosinophil cationic protein (ECP) levels by radioimmunoassay. Results: MOG specific responses in CSF showed significant increase in IL-5,IL-6, IgG, and IgM secreting cells in NMO patients compared w ith patients with RRMS, SPMS and healthy subjects. Interestingly,numbers of IgM secreting cells were significantly higher than identical specificity IgG secreti ng ones. Moreover,CSF Eo-2, Eo-3, and ECP levels were also significantly highe r in NMO patients compared to all three control populations.Anti-MOG IL-12 sec reting cells were increased in CSF and peripheral blood from NMO, RRMS, and SPMS patients when compared to healthy subjects. Conclusions: These observations suggest that neu romyelitis optica is associated with amajor humoral immune response (particularl y anti-MOG IgM production) and eosinophil activation present exclusively in CSF .
