To quantitatively study basic fibroblast growth factor(bFGF) in the vitreous of proliferative vitreoretinopathy(PVR) in order to understand the role of bFGF in the development of PVR.Method:High sensitive sandwich enz...To quantitatively study basic fibroblast growth factor(bFGF) in the vitreous of proliferative vitreoretinopathy(PVR) in order to understand the role of bFGF in the development of PVR.Method:High sensitive sandwich enzyme immunoassay technique(ELISA) was used to measure bFGF level in vitreous of normal eyes,the eyes of PVR-C or PVR-D grade,eyes of vitreous hemorrhage and the serum lkevels of bFGF in PVR-D patients.Results:The levels of bFGF in the vitreous were:median 5.20ng/L,quartile 15.47ng/L in 20 normal eyes;median 3.12ng/L,quartile 10.48ng/L in 35 PVR-C eyes;median 46.56ng/L,quartile 113.96ng/L in 26 PVR-D eyes;median 1.40ng/L,quartile 6.25ng/L in 25 vitreous hemorrhage eyes.The vitreous bFGF level in PVR-D group was significantly highter than that in the normal group ,PVR-C group and vitreous hemorrhage group(p<0.01).The mean of serum-bFGF level was 18.33±3.39ng/L.The vitroues bFGF level of PVR-D group was significantly higher than serum-bFGF level (P<0.01).And the vitreous-bFGF level in PVR-D group was significantly hgher in larger retinal tear subgroup.Concvlusion:The results suggested that bFGF is involved in the development of PVR.Eye Science 2000;16:7-10.展开更多
文摘To quantitatively study basic fibroblast growth factor(bFGF) in the vitreous of proliferative vitreoretinopathy(PVR) in order to understand the role of bFGF in the development of PVR.Method:High sensitive sandwich enzyme immunoassay technique(ELISA) was used to measure bFGF level in vitreous of normal eyes,the eyes of PVR-C or PVR-D grade,eyes of vitreous hemorrhage and the serum lkevels of bFGF in PVR-D patients.Results:The levels of bFGF in the vitreous were:median 5.20ng/L,quartile 15.47ng/L in 20 normal eyes;median 3.12ng/L,quartile 10.48ng/L in 35 PVR-C eyes;median 46.56ng/L,quartile 113.96ng/L in 26 PVR-D eyes;median 1.40ng/L,quartile 6.25ng/L in 25 vitreous hemorrhage eyes.The vitreous bFGF level in PVR-D group was significantly highter than that in the normal group ,PVR-C group and vitreous hemorrhage group(p<0.01).The mean of serum-bFGF level was 18.33±3.39ng/L.The vitroues bFGF level of PVR-D group was significantly higher than serum-bFGF level (P<0.01).And the vitreous-bFGF level in PVR-D group was significantly hgher in larger retinal tear subgroup.Concvlusion:The results suggested that bFGF is involved in the development of PVR.Eye Science 2000;16:7-10.
