基于pH调控多酚-唾液蛋白结合作用的三果汤含片涩味掩蔽方法研究

目的通过多酚-唾液蛋白络合作用的涩味形成关键机制,建立pH调控两者结合能力的三果汤含片涩味掩蔽新方法。方法采用荧光淬灭光谱法测定不同pH条件(2.5、3.5、4.5、5.5、6.5)下三果汤提取物与β-酪蛋白的相互作用。调节三果汤提取物pH至最佳条件,制备三果汤掩味含片。以电子舌涩味量化方法和志愿者视觉模拟量表法(visualanalogue scale,VAS)评价掩味效果。采用DPPH自由基清除能力、纸片扩散法评价含片的抗氧化活性和抑菌活性。结果荧光淬灭光谱测得三果汤提取物在pH 4.5时与β-酪蛋白的结合能力最弱,调节提取物pH至4.5制备三果汤掩味含片。掩味含片的涩度明显弱于原片;没食子酸的溶出度与原片基本一致;掩味含片的抗氧化活性和抑菌活性仅受到轻微影响。结论基于pH调控的多酚涩味掩蔽方法可行,解决了三果汤含片涩味突出的品质缺陷,对于多酚类中药口含片的涩味掩蔽提供了可行的技术方案。

Effect of acupuncture plus exercise on pain and motor function in patients with knee osteoarthritis

Objective:To observe the effects of acupuncture combined with isokinetic eccentric exercise on pain and motor function in patients with knee osteoarthritis(KOA).Methods:Sixty-four patients with KOA were randomly divided into an observation group and a control group,with 32 cases in each group.The observation group received acupuncture and isokinetic eccentric exercise,and the control group received isokinetic eccentric exercise and oral intake of meloxicam tablets.Both groups were treated for 4 weeks.Before and after treatment,the visual analog scale(VAS)score,the lysholm knee scale(LKS)score,and the quadriceps peak torque(PT)were evaluated.Results:The total effective rate was 93.8%in the observation group,versus 81.3%in the control group.The difference between the two groups was statistically significant(P<0.05).After treatment,the VAS scores of the two groups were lower than those before the treatment;both LKS score and quadriceps PT were higher than those before treatment,and the differences were statistically significant(all P<0.05).The VAS score,LKS score and quadriceps PT(angular velocity was 60%)of the observation group were significantly different from those of the control group(all P<0.05).Conclusion:Acupuncture plus isokinetic eccentric exercise is effective in treating KOA.The combined treatment can reduce the pain of KOA patients,improve joint function,strengthen quadriceps,improve joint stability,and its curative effect is better than isokinetic eccentric exercise plus oral intake of meloxicam tablets.

Warm needling moxibustion plus functional exercises for knee osteoarthritis

Objective： To observe the clinical efficacy of warm needling moxibustion plus functional exercises in treating knee osteoarthritis（KOA）. Methods： Totally 108 eligible KOA patients were intervened by warm needling moxibustion plus functional exercises. The warm needling moxibustion was given 3 times a week, successively for 4 weeks. The visual analogue scale（VAS） was adopted to evaluate the pain intensity of the knee joint. After 4-week treatment, the change of VAS score, range of motion（ROM） of knee, and Lysholm score were observed, and the clinical efficacy was also estimated. Results： After treatment, the VAS score dropped obviously, and the ROM and Lysholm score increased markedly; the total effective rate was 83.3%. Conclusion： Warm needling moxibustion plus functional exercises is effective in treating KOA, as it can relieve the knee joint pain and improve the joint motion.

Effects of acupuncture plus spinal manipulations on physical functioning and biochemical indicators in patients with ankylosing spondylitis

Objective To observe the effects of acupuncture plus spinal manipulations on the physical functioning and levels of alkaline phosphatase(ALP),erythrocyte sedimentation rate(ESR),C-reactive protein(CRP)and osteoprotegerin(OPG)in patients with ankylosing spondylitis(AS).Methods A total of 128 AS cases were allocated into a control group and an observation group using random number table method,with 64 cases in each group.Patients in both groups took sulfasalazine and meloxicam.Patients in the observation group received additional acupuncture plus spinal manipulations.The efficacy,Bath AS functional index(BASFI),Bath AS disease activity index(BASDAI),and the levels of ALP,ESR,CRP and OPG were compared between the two groups after eight weeks of treatment.Results After treatment,the symptom scores of traditional Chinese medicine in both groups were decreased(all P<0.05),and these scores in the observation group were significantly lower than in the control group(all P<0.05);the VAS,BASFI and BASDAI scores in both groups were decreased(all P<0.05),and these scores in the observation group were significantly lower than in the control group(all P<0.05);and the ALP,ESR,CRP and OPG levels in both groups were decreased(all P<0.05),and these levels in the observation group were significantly lower than in the control group(all P<0.05).The total efficacy rate was 92.2%in the observation group,versus 78.1%in the control group,presenting a statistical significance(P<0.05).Conclusion Conventional medication combined with acupuncture and spinal manipulations can improve clinical symptoms,accelerate the recovery of physical functioning,and reduce the ALP,ESR,CRP and OPG levels.

Clinical observation of sinew-regulating bone-setting manipulations plus exercise therapy for chronic non-specific low back pain

Objective:To evaluate the clinical efficacy of sinew-regulating bone-setting manipulations plus exercise therapy in treating chronic non-specific low back pain(CNLBP).Methods:A total of 65 CNLBP patients were divided into two groups by the random number table method.Thirty-three cases in the treatment group were intervened by sinew-regulating bone-setting manipulations plus exercise therapy;32 cases in the control group were intervened by medium-frequency electrotherapy plus exercise therapy.Before and after treatment,visual analog scale(VAS),dynamic and static muscle endurance of low back,median frequency(MF)of surface electromyography(sEMG)and Oswestry disability index(ODI)were used to evaluate the low back function.The therapeutic efficacy was estimated after treatment.Results:The two groups each had 2 dropouts during the study.The total effective rate was 90.3%in the treatment group versus 66.7%in the control group,and the between-group difference was statistically significant(P<0.05).After treatment,the VAS score,dynamic and static muscle endurance of low back,MF of sEMG and ODI score all changed significantly in both groups(all P<0.05);all the items in the treatment group were significantly different from those in the control group(all P<0.05).Conclusion:Sinew-regulating bone-setting manipulations plus exercise therapy can effectively release pain in CNLBP patients,increase muscle endurance of the low back and improve the quality of life,and its therapeutic efficacy is more significant than that of medium-frequency electrotherapy plus exercise therapy.

Therapeutic effect of warm needling plus acupoint sticking therapy on primary dysmenorrhea due to cold-dampness retention

Objective： To observe the clinical efficacy of warm needling plus acupoint sticking therapy for primary dysmenorrhea. Methods： A total of 60 eligible cases were randomly allocated into a treatment group （n=30） and a control group （n=30） according to their sequence of consultation. Patients in the treatment group were treated with warm needling combined with acupoint sticking therapy, whereas patients in the control group were treated with oral Ibuprofen （Fenbid） capsules. After two menstrual cycles, the visual analogue scale （VAS） score, COX menstrual symptom scale （CMSS） and clinical efficacy were compared between the two groups. Results： After treatment, the VAS and CMSS scores were significantly reduced in both groups, indicating that both treatment protocols can remarkably alleviate pain. There was a between-group statistical difference in comparing the CMSS score （P〈0.05）, but no between-group statistical difference in the VAS score （P〉0.05）. However, the follow-up visit showed between-group statistical differences in comparing VOA and CMSS scores （both P〈0.01）. The clinical effect, relapse rate and long-term efficacy in the treatment group were better than those in the control group. Conclusion： Warm needling plus acupoint sticking therapy is a simple but effective therapy for primary dysmenorrhea. In addition, it causes fewer cases with relapse.