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不同pH条件下细菌视紫红质的共振拉曼光谱研究 被引量:1
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作者 杨景文 孙永泰 +2 位作者 张极震 朱克莉 刘芳 《生物物理学报》 CAS CSCD 北大核心 1989年第3期310-313,共4页
本实验测定了不同pH条件下嗜盐菌紫膜中细菌视紫红质(bR)的共振拉曼光谱.13-顺式视黄醛生色团的特征峰1187cm^(-1)和全反式、13-顺式共有的特征峰1200cm^(-1)带强度之比I_(1187)/I_(1200)在pH1.0-8.9之间约为0.76,而pH高于8.9为0.97.pH3... 本实验测定了不同pH条件下嗜盐菌紫膜中细菌视紫红质(bR)的共振拉曼光谱.13-顺式视黄醛生色团的特征峰1187cm^(-1)和全反式、13-顺式共有的特征峰1200cm^(-1)带强度之比I_(1187)/I_(1200)在pH1.0-8.9之间约为0.76,而pH高于8.9为0.97.pH3.0-9.0时C=NH^+振动峰为1640-1642cm^(-1),pH9.4以上为1642-1644cm^(-1),pH9.2附近变化最大,pH3.0以下低于1640cm^(-1).酸性和弱碱性范围时,19-CH_3和20-CH_3的面内变形振动与面外变形振动相互重叠,碱性范围分为双峰.并讨论了对结构及其稳定性的影响. 展开更多
关键词 视质红质 共振 拉曼光谱 细菌 PH
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Preparation of a cross-linked gelatin/bacteriorhodopsin film and its photochromic properties 被引量:1
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作者 WANG YaZhuo WU Jia +1 位作者 MA DeWang DING JianDong 《Science China Chemistry》 SCIE EI CAS 2011年第2期405-409,共5页
Bacteriorhodopsin (BR) is a photochromic membrane protein isolated from a strain of halobacteria.Embedment of BR into a polymeric matrix enables the application of the photoactive protein as an optical material.In thi... Bacteriorhodopsin (BR) is a photochromic membrane protein isolated from a strain of halobacteria.Embedment of BR into a polymeric matrix enables the application of the photoactive protein as an optical material.In this work,a chemically crosslinked BR/gelatin film was prepared.The cross-linked film was found to be highly stable even under extreme alkaline or detergent circumstance while BR maintained its bioactivity.The treatments of base and detergents also led to dramatic prolongation of the lifetime of M photoproduct,which might be beneficial for potential applications such as information storage.The BR/gelatin film was demonstrated to tentatively record a simple pattern. 展开更多
关键词 BACTERIORHODOPSIN photochromic protein natural macromolecule functional polymer photoresponsive materials
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