食管鳞状上皮内瘤变的临床病理学特征分析

目的探讨食管鳞状上皮内瘤变的临床病理学特征,准确把握“细胞异型性”和“组织结构异型性”,为临床病理医师正确诊断食管鳞状上皮内瘤变提供理论依据。方法选取食管鳞状上皮内瘤变典型病例162例,收集患者临床资料并进行比较,光镜下观察食管鳞状上皮内瘤变的典型表现,总结可能提示瘤变的临床病理学特征,并分析其形成的病理原因。结果162例食管鳞状上皮内瘤变患者中男女比例为126∶36,年龄(61.48±9.16)岁。在年龄分组中,经Mann-Whitney检验可知,不同年龄食管鳞状上皮内瘤变级别比较差异有统计学意义(Z=-2.911,P=0.0036)。与<61岁组相比,食管鳞状上皮内瘤变在≥61岁人群更易发生。在镜下观察中发现可能提示食管鳞状上皮内瘤变的临床病理学特征,如核质比增大,核分裂像增多;表层角化颗粒及有核细胞增多;瘤变细胞胞质嗜酸性增强;基底层出现皮脂腺样细胞;瘤变细胞平行或斜行生长;细胞排列紊乱、极向消失;成熟梯度消失;异常角化;上皮内血管增生;上皮脚延伸或推挤式生长。结论食管鳞状上皮内瘤变在≥61岁男性中更易发生;光镜下可能提示瘤变的临床病理学特征,可以帮助临床病理医师较为容易的把握食管上皮内瘤变的正确诊断。

Transient Expression of Human Papillomavirus Type 16(HPV 16) mRNA in Normal Human Keratinocytes Transfected by pSV2-neo/16

Objective: To investigate the expression of HPV16 mRNA innormal human keratinocytes transfected with pSV2-neo/16. Methods: First human keratinocytes were cultured in theserum-free medium M154.Second, the plasmid pSV2-neo/16was transfected into the human keratinocytes using atransfecting reagent. Third, RT-PCR and Southern Blottingwere used to detect the expression of HPV16 mRNA and DNAin the transfected keratinocytes, respectively. Results: The expression of HPV 16 mRNA was successfullyamplified and an 110bp was detected by RT-PCR. A 7.9kbfragment was confirmed in the transfected keratinocytes bySouthern Blot analysis. Conclusion: HPV 16 mRNA and DNA were successfullydetected in the human keratinocytes.