SHAAN6-17 and 2-17-20, two moniliformin producing segregants of Fusarium subglutinans, were obtained by isolating single spores from SHAAN6 and 2-17 respectively. SHAAN6-17 forms sectors frequently but 2-17-20 does no...SHAAN6-17 and 2-17-20, two moniliformin producing segregants of Fusarium subglutinans, were obtained by isolating single spores from SHAAN6 and 2-17 respectively. SHAAN6-17 forms sectors frequently but 2-17-20 does not. Ninety-five segregants were isolated from SHAAN6-17 by isolating single spores. All these segregants continued forming sectors and also maintained their ability of producing moniliformin. When cultivated under different pH, from 5.0 to 9.0, there were no significant differences among the segregants in sector formation. At last, by using CF11 cellulose method a kind of dsRNA was purified from SHAAN6-17, but this dsRNA was not purified from 2-17-20. It is suggested that the course of SHAAN6-17’s sector formation does not due to the mutations in its genome or the environmental pH, but maybe due to some kind of mycovirus.展开更多
文摘SHAAN6-17 and 2-17-20, two moniliformin producing segregants of Fusarium subglutinans, were obtained by isolating single spores from SHAAN6 and 2-17 respectively. SHAAN6-17 forms sectors frequently but 2-17-20 does not. Ninety-five segregants were isolated from SHAAN6-17 by isolating single spores. All these segregants continued forming sectors and also maintained their ability of producing moniliformin. When cultivated under different pH, from 5.0 to 9.0, there were no significant differences among the segregants in sector formation. At last, by using CF11 cellulose method a kind of dsRNA was purified from SHAAN6-17, but this dsRNA was not purified from 2-17-20. It is suggested that the course of SHAAN6-17’s sector formation does not due to the mutations in its genome or the environmental pH, but maybe due to some kind of mycovirus.
