目的观察角膜绷带镜对生物工程角膜移植术后的临床治疗作用。方法收集我院2017年1月至2018年6月住院并使用生物工程角膜进行板层角膜移植手术治疗的感染性角膜溃疡患者30例30眼的临床资料,随机分为A、B两组,各15例。A组角膜上皮愈合后...目的观察角膜绷带镜对生物工程角膜移植术后的临床治疗作用。方法收集我院2017年1月至2018年6月住院并使用生物工程角膜进行板层角膜移植手术治疗的感染性角膜溃疡患者30例30眼的临床资料,随机分为A、B两组,各15例。A组角膜上皮愈合后给予戴角膜绷带镜联合滴眼液点眼治疗,2周更换1次,术后3个月拆线后停用。B组患者不戴角膜绷带镜,药物治疗同A组。观察生物工程角膜移植术后角膜植片上皮化时间,比较术后第1、2、3、4周两组角膜植片水肿程度,比较术后第1、2、3个月两组角膜植片上皮完整性和泪膜破裂时间(breakup time of tear film,BUT),比较两组角膜植片水肿消退时间和术后3个月两组角膜植片存活率。结果术后3~5 d角膜植片上皮化,2~4周植片水肿消退。A组患者术后第2~3周角膜植片水肿消退,B组患者第3~4周角膜植片水肿消退,时间长于A组(P <0.001),术后第2~3周A组角膜植片水肿程度明显轻于B组(第2周P=0.002,第3周P <0.001)。术后第1、2、3个月时A组患者角膜荧光素染色评分均低于B组(均P <0.001),BUT时长高于B组(P=0.009、0.002、0.33)。A组患者中1例未按时复诊戴角膜绷带镜近5周,继发细菌感染,退出研究,术后3个月A组植片存活率93.3%(14/15);B组患者有2例1个月后出现角膜上皮无菌性缺损,另有1例术后2个月出现角膜溶解,3例均退出研究,术后3个月B组植片存活率80.0%(12/15),与A组相比差异无统计学意义(P=0.283)。结论生物工程角膜上皮化后戴角膜绷带镜可加速角膜植片水肿消退,保护角膜上皮完整性,维持眼表泪液稳定,有利于维护眼表微环境健康,对生物工程角膜术后角膜植片修复有明显促进作用。展开更多
Abstract:Objective To establish a method for culturing normal human oral keratinocytes.Methods Specimens obtained from healthy humans undergoing oral surgery were dissociated into single cell suspensions by dispase an...Abstract:Objective To establish a method for culturing normal human oral keratinocytes.Methods Specimens obtained from healthy humans undergoing oral surgery were dissociated into single cell suspensions by dispase and trypsin. The cells were grown in serum-free medium. Morphological characteristics were studied under light microscope and electron microscope. Cytokeratins were shown by immunohistochemistry.Results Cells could be maintained in culture up to 4-5 passages or 30-50 days. Electron microscope revealed that there were desmosomes and tonofibrils in the oral keratinocytes. The cells showed positive staining for cytokeratin antibody. Conclusion Human oral keratinocytes have been successfully grown in serial culture.展开更多
文摘目的观察角膜绷带镜对生物工程角膜移植术后的临床治疗作用。方法收集我院2017年1月至2018年6月住院并使用生物工程角膜进行板层角膜移植手术治疗的感染性角膜溃疡患者30例30眼的临床资料,随机分为A、B两组,各15例。A组角膜上皮愈合后给予戴角膜绷带镜联合滴眼液点眼治疗,2周更换1次,术后3个月拆线后停用。B组患者不戴角膜绷带镜,药物治疗同A组。观察生物工程角膜移植术后角膜植片上皮化时间,比较术后第1、2、3、4周两组角膜植片水肿程度,比较术后第1、2、3个月两组角膜植片上皮完整性和泪膜破裂时间(breakup time of tear film,BUT),比较两组角膜植片水肿消退时间和术后3个月两组角膜植片存活率。结果术后3~5 d角膜植片上皮化,2~4周植片水肿消退。A组患者术后第2~3周角膜植片水肿消退,B组患者第3~4周角膜植片水肿消退,时间长于A组(P <0.001),术后第2~3周A组角膜植片水肿程度明显轻于B组(第2周P=0.002,第3周P <0.001)。术后第1、2、3个月时A组患者角膜荧光素染色评分均低于B组(均P <0.001),BUT时长高于B组(P=0.009、0.002、0.33)。A组患者中1例未按时复诊戴角膜绷带镜近5周,继发细菌感染,退出研究,术后3个月A组植片存活率93.3%(14/15);B组患者有2例1个月后出现角膜上皮无菌性缺损,另有1例术后2个月出现角膜溶解,3例均退出研究,术后3个月B组植片存活率80.0%(12/15),与A组相比差异无统计学意义(P=0.283)。结论生物工程角膜上皮化后戴角膜绷带镜可加速角膜植片水肿消退,保护角膜上皮完整性,维持眼表泪液稳定,有利于维护眼表微环境健康,对生物工程角膜术后角膜植片修复有明显促进作用。
文摘Abstract:Objective To establish a method for culturing normal human oral keratinocytes.Methods Specimens obtained from healthy humans undergoing oral surgery were dissociated into single cell suspensions by dispase and trypsin. The cells were grown in serum-free medium. Morphological characteristics were studied under light microscope and electron microscope. Cytokeratins were shown by immunohistochemistry.Results Cells could be maintained in culture up to 4-5 passages or 30-50 days. Electron microscope revealed that there were desmosomes and tonofibrils in the oral keratinocytes. The cells showed positive staining for cytokeratin antibody. Conclusion Human oral keratinocytes have been successfully grown in serial culture.
