The application of Penman's method of cell fractionation to plant protoplasts leads to our finding of keratin intermediate filament(IF)-like system in maize protoplasts,which was identified by using immunogold lab...The application of Penman's method of cell fractionation to plant protoplasts leads to our finding of keratin intermediate filament(IF)-like system in maize protoplasts,which was identified by using immunogold labelling with monoclonal antibody of cytokeratin from animal cells.Many gold particles were found to be bound on filaments,linked by 3 nm filaments.After further digestion and extraction with DNase I and ammonium sulphate.IF-like framework-lamina-nuclear matrix system was shown under electron microscope.That IF system exists in plant protoplasts just like in animal cells,and their main component is keratin-like protein.展开更多
The aim of this research was to develop the porous spherical cellulose production from corn cob as an exfoliating agent for cosmetic industries. In process development of the porous spherical cellulose production from...The aim of this research was to develop the porous spherical cellulose production from corn cob as an exfoliating agent for cosmetic industries. In process development of the porous spherical cellulose production from corn cob, it was found that alkaline extraction with 10% sodiumhydroxide gave 85.86% purity of cellulose and the crystallinity index of the pulp of 76.08%. Then, the obtained pulp was extracted with acid sodium chlorite. It was found that the acid sodium chlorite extraction increased the cellulose from 85.86% to 91.86%. The obtained pulp was hydrolyse dwithdilute hydrochloric solution. The result shows that the crystallinity increased from 71.13% to 86.31% with smaller crystallite size compared to that obtained acid sodium chlorite extraction. The hydrolysis pulp was used to prepare porous spherical cellulose which has homogeneous and rough surface. The porosity and pore volume of the porous spherical cellulose increased when the amount of calcium carbonate as porogen was increased. The porous spherical cellulose was prepared from the optimized conditions and the physical and chemical properties were analysed and compared with standard commercial beads (Sephadex G-25). It was found that the porous spherical cellulose had particle size and pH value similar to the standard value. Hazardous substances such as arsenic, lead and mercury were not found in the obtained porous spherical cellulose. The consumers test shows that 87~/'0 of consumers accepted the product and the overall complacency was great.展开更多
文摘The application of Penman's method of cell fractionation to plant protoplasts leads to our finding of keratin intermediate filament(IF)-like system in maize protoplasts,which was identified by using immunogold labelling with monoclonal antibody of cytokeratin from animal cells.Many gold particles were found to be bound on filaments,linked by 3 nm filaments.After further digestion and extraction with DNase I and ammonium sulphate.IF-like framework-lamina-nuclear matrix system was shown under electron microscope.That IF system exists in plant protoplasts just like in animal cells,and their main component is keratin-like protein.
文摘The aim of this research was to develop the porous spherical cellulose production from corn cob as an exfoliating agent for cosmetic industries. In process development of the porous spherical cellulose production from corn cob, it was found that alkaline extraction with 10% sodiumhydroxide gave 85.86% purity of cellulose and the crystallinity index of the pulp of 76.08%. Then, the obtained pulp was extracted with acid sodium chlorite. It was found that the acid sodium chlorite extraction increased the cellulose from 85.86% to 91.86%. The obtained pulp was hydrolyse dwithdilute hydrochloric solution. The result shows that the crystallinity increased from 71.13% to 86.31% with smaller crystallite size compared to that obtained acid sodium chlorite extraction. The hydrolysis pulp was used to prepare porous spherical cellulose which has homogeneous and rough surface. The porosity and pore volume of the porous spherical cellulose increased when the amount of calcium carbonate as porogen was increased. The porous spherical cellulose was prepared from the optimized conditions and the physical and chemical properties were analysed and compared with standard commercial beads (Sephadex G-25). It was found that the porous spherical cellulose had particle size and pH value similar to the standard value. Hazardous substances such as arsenic, lead and mercury were not found in the obtained porous spherical cellulose. The consumers test shows that 87~/'0 of consumers accepted the product and the overall complacency was great.