Peltophorum dubium seeds provided by Anhembi, SP were scarified in 98% H2SO4 for 15 min to overcome mechani-cal dormancy. Seeds were primed in solutions of 0.2% Captan at 10 C and 27C, PEG 6000 -1.0 MPa at 10C and 27...Peltophorum dubium seeds provided by Anhembi, SP were scarified in 98% H2SO4 for 15 min to overcome mechani-cal dormancy. Seeds were primed in solutions of 0.2% Captan at 10 C and 27C, PEG 6000 -1.0 MPa at 10C and 27C, 0.5 mol KNO3, 0.75 Mol KNO3, 1.0 Mol KNO3. Eight treatments including the primed seeds and nonprimed seeds, five replicates with 100 seeds for each treatment, were set to 15-cm-Petri dish with double filter paper moistened with testing solution PEG in refrigerator at 27C. For the experiments of all the groups, osmotic potential were 0.0, -0.2, -0.4, -0.6, -0.8, -1.0, -1.2, and -1.4 MPa. P. dubium seeds were also set to water stress experiment in rolled paper with PEG solutions from 0.0 to -1.0 M Pa. Germination percentage decreased with the increase of PEG concentration. Control group had a better germination percentage than other groups. Germination hardly occurred in PEG -1.4 MPa.展开更多
The factors influencing dormancy release in lily bulbs strongly affect commercialization success, but the mechanism of dormancy release is still unclear. Magnetic resonance imaging (MRI) can detect changes in morpholo...The factors influencing dormancy release in lily bulbs strongly affect commercialization success, but the mechanism of dormancy release is still unclear. Magnetic resonance imaging (MRI) can detect changes in morphology and water status in a living plant bulb and aid in investigating release factors. To evaluate whether MRI could be used to detect intra-bulb metabolic changes during the dormant period in Oriental Lilies (Lilium 'Sorbonne'), a series of MRI and sugar concentration measurements were performed weekly on bulbs stored for 11 weeks at 4°C. The image quality of intra-bulb structure obtained using T 1-weighted imaging was superior to that obtained using T 2 -weighted imaging and had a higher signal-to-noise ratio (0.97±0.01). Magnetization transfer ratio values for the bud and basal plate declined during the first eight weeks of cold storage (P>0.05), and were well correlated with concentration of soluble sugar in the bud (R 2 =0.95) and basal plate (R 2 =0.93). Thus, MRI can serve as a valuable tool for observation and analysis of dynamic morphological and metabolic changes in vivo during dormancy release. This information is potentially useful as a guide in the improvement of horticultural product quality.展开更多
基金This work is Supported by CAPES Brazil. Open research laboratory of forest plant ecology NEFU. The State's tenth five-year "211 Project"-supported key academic discipline program of ECNU。
文摘Peltophorum dubium seeds provided by Anhembi, SP were scarified in 98% H2SO4 for 15 min to overcome mechani-cal dormancy. Seeds were primed in solutions of 0.2% Captan at 10 C and 27C, PEG 6000 -1.0 MPa at 10C and 27C, 0.5 mol KNO3, 0.75 Mol KNO3, 1.0 Mol KNO3. Eight treatments including the primed seeds and nonprimed seeds, five replicates with 100 seeds for each treatment, were set to 15-cm-Petri dish with double filter paper moistened with testing solution PEG in refrigerator at 27C. For the experiments of all the groups, osmotic potential were 0.0, -0.2, -0.4, -0.6, -0.8, -1.0, -1.2, and -1.4 MPa. P. dubium seeds were also set to water stress experiment in rolled paper with PEG solutions from 0.0 to -1.0 M Pa. Germination percentage decreased with the increase of PEG concentration. Control group had a better germination percentage than other groups. Germination hardly occurred in PEG -1.4 MPa.
基金supported by the Special Fund for Agro-scientific Research in the Public Interest (Grant No. 200903020)
文摘The factors influencing dormancy release in lily bulbs strongly affect commercialization success, but the mechanism of dormancy release is still unclear. Magnetic resonance imaging (MRI) can detect changes in morphology and water status in a living plant bulb and aid in investigating release factors. To evaluate whether MRI could be used to detect intra-bulb metabolic changes during the dormant period in Oriental Lilies (Lilium 'Sorbonne'), a series of MRI and sugar concentration measurements were performed weekly on bulbs stored for 11 weeks at 4°C. The image quality of intra-bulb structure obtained using T 1-weighted imaging was superior to that obtained using T 2 -weighted imaging and had a higher signal-to-noise ratio (0.97±0.01). Magnetization transfer ratio values for the bud and basal plate declined during the first eight weeks of cold storage (P>0.05), and were well correlated with concentration of soluble sugar in the bud (R 2 =0.95) and basal plate (R 2 =0.93). Thus, MRI can serve as a valuable tool for observation and analysis of dynamic morphological and metabolic changes in vivo during dormancy release. This information is potentially useful as a guide in the improvement of horticultural product quality.
