Objectives: To determine the susceptibilities of M.hominis and U. urealyticum to fluoroquinolones forthe instruction of reasonable clinical application ofantibiotics.Method: The susceptibilities of M. hominis and U.ur...Objectives: To determine the susceptibilities of M.hominis and U. urealyticum to fluoroquinolones forthe instruction of reasonable clinical application ofantibiotics.Method: The susceptibilities of M. hominis and U.urealyticum to six fluoroquinolones were determinedby the broth dilution method.Results: Sparfloxacin and gatifloxacin were veryactive with MIC50S of 0.03125 and 0.25 μg/ml againstM. hominis, 0.25 and 0.5 μg/ml against U. urealyticum,respectively. Levofloxacin and ofloxacin had MIC50S of1 μg/ml and 2 μg/ml, respectively against both species.Norfloxacin was less effective against both species at16 and 32 μg/ml. Ciprofloxacin was unusual in thatthe MIC50S varied fourfold between species, with 2 μg/ml against M. hominis and 8 μg/ml against U.urealyticum.Conclusions: The results can provide useful infor-mation for selecting fluoroquinolones for treatmentof urogenital mycoplasma infections.展开更多
Two alternative formulations of single crystal plasticity model were introduced respectively and two schemes were implemented in the explicit FE code with software ABAQUS/Explicit by writing the user subroutine VUMAT....Two alternative formulations of single crystal plasticity model were introduced respectively and two schemes were implemented in the explicit FE code with software ABAQUS/Explicit by writing the user subroutine VUMAT.Meshes containing material data were created with solid elements.Each element represented an individual grain,and the grain orientations were explicitly stored and updated at each increment.Tangential modulus method was employed to calculate the plastic shear strain increment of deformation systems in combination with a hardening law to describe the hardening responses.Both two developed subroutines were applied to simulate the texture evolution during the uniaxial tension of copper(FCC),cold rolling of IF steel(BCC) and uniaxial compression of AZ31 magnesium alloy(HCP).The predicted texture distributions are in qualitative agreement with the experimental results.展开更多
In this paper, we prove the existence in H+^2, an incomplete metric subspace of H^2×H^2×H^2, of global solutions to the system for a one-dimensional non-monotone fluid in bounded domainΩ=(0,1). The resul...In this paper, we prove the existence in H+^2, an incomplete metric subspace of H^2×H^2×H^2, of global solutions to the system for a one-dimensional non-monotone fluid in bounded domainΩ=(0,1). The results in this paper have improved those previously related results.展开更多
An emulsion-congealing technique is used to prepare solid lipid microparticles (SLM) containing ibuprofen with glyceryl behenate, tripalmitin and beewax as excipients. The difference of the solubility parameters bet...An emulsion-congealing technique is used to prepare solid lipid microparticles (SLM) containing ibuprofen with glyceryl behenate, tripalmitin and beewax as excipients. The difference of the solubility parameters between the excipients and ibuprofen are used to analyze their compatibility. Both the solubility parameter analysis and the experimental results show that glyceryl behenate is the best among the three excipients. The solid particles disperse well in aqueous phase when the drug loading reaches 10% (relative to lipid only). Glycerides exhibit marked polymorphism and their rapid rates of crystallization accelerate the formation of metastable crystal modification. The metastable crystal modification characterizes high drug loading capacity but less stability. Increasing the content of lipophilic drug in a lipid matrix facilitates the transformation of excipients to more stable polymorphic forms.展开更多
Through the Wronskian technique, a simple and direct proof is presented that the AKNS hierarchy in the bilinear form has generalized double Wronskian solutions. Moreover, by using a unified way, soliton solutions, rat...Through the Wronskian technique, a simple and direct proof is presented that the AKNS hierarchy in the bilinear form has generalized double Wronskian solutions. Moreover, by using a unified way, soliton solutions, rational solutions, Matveev solutions and complexitons in double Wronskian form for it are constructed.展开更多
A combined first and second-order model, which includes bulk decay and wall decay, was developed to describe chlorine decay in water distribution systems. In the model the bulk decay has complex relationships with tot...A combined first and second-order model, which includes bulk decay and wall decay, was developed to describe chlorine decay in water distribution systems. In the model the bulk decay has complex relationships with total organic carbon (TOC), the initial chlorine concentration and the temperature. Except for the initial stages they can be simplified into a linear increase with TOC, a linear decrease with initial chlorine concentration and an exponential relationship with the temperature. The model also explains why chlorine decays rapidly in the initial stages. The parameters of model are determined by deriving the best fitness with experimental data. And the accuracy of model has been verified by using the experimental data and the monitoring data in a distribution system.展开更多
Various busbar configurations were built and modeled by the custom code based on the commercial package ANSYS for the 500 kA aluminum electrolysis cell.The configuration parameters,such as side riser entry ratio,numbe...Various busbar configurations were built and modeled by the custom code based on the commercial package ANSYS for the 500 kA aluminum electrolysis cell.The configuration parameters,such as side riser entry ratio,number of cathode bars connected to each riser,vertical location of side cathode busbar and short side cathode busbar,distance between rows of cells in potline,the number of neighboring cells,ratio of compensation busbar carried passing under cell and its horizontal location under cell along with large magnetohydrodynamic(MHD) computation based on the custom evaluation function were simulated and discussed.The results show that a cell with riser entry ratio of 11:9:8:9:11 and cathode busbar located at the level of aluminum solution,50% upstream cathode current passing under cell for magnetic field compensation,the distance between rows of 50 m is more stable.展开更多
Hemopericardium is a common finding at autopsy, but it may represent a challenge for the forensic pathologist when the etiopathologi-cal relationship in causing death is requested. Hemopericardium and cardiac tamponad...Hemopericardium is a common finding at autopsy, but it may represent a challenge for the forensic pathologist when the etiopathologi-cal relationship in causing death is requested. Hemopericardium and cardiac tamponade can be evaluated in living people using radiological techniques, in particular computer tomography (CT). Only a few studies are reported in literature involving post-mortem (PM) cases, where PMCT imaging has been used in order to investigate acute hemopericardium, and they have shown a good accuracy of this technique. Here we report a case involving a 70-year-old white male found dead on the beach, with a medical history of hepatitis C and chronic hypertension with a poor pharmacological response. A PMCT was performed about 3 h after the discovery of the body. The PMCT examination showed an intrapericardial aortic dissection associated to a periaortic hematoma, a sickle-shaped intramural hematoma, a false lumen, and a hemop-ericardium consisting in fluid and clotted blood. In this case, the PMCT was able to identify the cause of death, even though a traditional autopsy was required to confirm the radiological findings. PMCT is a reliable technique, which in chosen cases, can be performed without the need for a traditional autopsy to be carried out.展开更多
A theoretical model on the solvus line prediction of a film was proposed and applied to a nanogranular A1-Cu system. The calculation results show that the solvus line of solute Cu will markedly lower with the decrease...A theoretical model on the solvus line prediction of a film was proposed and applied to a nanogranular A1-Cu system. The calculation results show that the solvus line of solute Cu will markedly lower with the decrease of grain size, namely, the starting temperature of θ (Al2Cu) precipitation in a nanogranular A1-Cu film will markedly lower than that of conventional coarse grain alloy with the same Cu concentration, and the precipitation temperatures calculated are comparable with the experimental ones. The theoretical model can be simply used to calculate the starting temperature of precipitation in A1-Cu films under three states: ① films with substrate; ②films without substrate; ③ultrafine grain bulk alloy. As a result, the model is universal, moreover, can be, in principle, used to predict precipitation temperature in other systems.展开更多
This paper deals with the existence and nonexistence of global positive solutions of the following quasilinear parabolic equations:u t=1m△u m-u n, x∈Ω,t>0 1m·u mv=u p,x∈Ω,t>0 u(x,0)=u 0(x)>0,x∈Ω-w...This paper deals with the existence and nonexistence of global positive solutions of the following quasilinear parabolic equations:u t=1m△u m-u n, x∈Ω,t>0 1m·u mv=u p,x∈Ω,t>0 u(x,0)=u 0(x)>0,x∈Ω-where Ω∈R N is a bounded domain with smooth boundary Ω,m,n,p are positive constants, γ is the outward normal vector. The necessary and sufficient conditions for the global existence of solutions are obtained.展开更多
Generation of induced pluripotent stem (iPS) cells from somatic cells has been achieved successfully by simultaneous viral transduction of defined reprogramming transcription factors (TFs). However, the process re...Generation of induced pluripotent stem (iPS) cells from somatic cells has been achieved successfully by simultaneous viral transduction of defined reprogramming transcription factors (TFs). However, the process requires multiple viral vectors for gene delivery. As a result, generated iPS cells harbor numerous viral integration sites in their genomes. This can increase the probability of gene mutagenesis and genomic instability, and present significant barriers to both research and clinical application studies of iPS cells. In this paper, we present a simple lentivirus reprogramming system in which defined factors are fused in-frame into a single open reading frame (ORF) via self-cleaving 2A sequences. A GFP marker is placed downstream of the transgene to enable tracking of transgene expression. We demonstrate that this polycistronic expression system efficiently generates iPS cells. The generated iPS cells have normal karyotypes and are similar to mouse embryonic stem cells in morphology and gene expression. Moreover, they can differentiate into cell types of the three embryonic germ layers in both in vitro and in vivo assays. Remarkably, most of these iPS cells only harbor a single copy of viral vector. This system provides a valuable tool for generation of iPS cells, and our data suggest that the balance of expression of transduced reprogramming TFs in each cell is essential for the reprogramming process. More importantly, when delivered by non-integrating gene-delivery systems, this re-engineered single ORF will facilitate efficient generation of human iPS cells free of genetic modifications.展开更多
TiO2 nanoparticles were prepared using the hydrothermal method and modified with CgN to syn-thesize a Type-Ⅱheterojunction semiconductor photocatalyst,TiO2-C;Na.In addition,a carbon layerwas coated onto the TiO2 nano...TiO2 nanoparticles were prepared using the hydrothermal method and modified with CgN to syn-thesize a Type-Ⅱheterojunction semiconductor photocatalyst,TiO2-C;Na.In addition,a carbon layerwas coated onto the TiO2 nanoparticles and the obtained material was uniformly covered on thesurface of CaNa to form an all-solid-state Z-scheme semiconductor photocatalyst,TiO2-C-C3N4,Through characterization by XRD,XPS,SEM,TEM,BET,photoelectrochemical experiments,UV-visible diffuse reflection,and PL spectroscopy,the charge transfer mechanism and band gappositions for the composite photocatalysts were analyzed.The Type-Ⅱand all-solid-state Z-schemeheterojunction structures were compared.By combining microscopic internal mechanisms withmacroscopic experimental phenomena,the relationship between performance and structure wasverified.Experimental methods were used to explore the adaptation degree of different photocata-lytic mechanisms using the same degradation system.This study highlights effective photocatalystdesign to meet the requirements for specific degradation conditions.展开更多
AIM: To investigate the inhibitory effect of VP22 fusion protein-based dominant negative (DN) mutant on Hepatitis Bvrus (HBV) replication. METHODS: Full-length or truncated fragment of VP22 was fused to C termin...AIM: To investigate the inhibitory effect of VP22 fusion protein-based dominant negative (DN) mutant on Hepatitis Bvrus (HBV) replication. METHODS: Full-length or truncated fragment of VP22 was fused to C terminal of HBV core protein (HBc), and subcloned into pcDNA3.1 (-) vector, yielding eukaryotic expression plasmids of DN mutant. After transfection into HepG2.2.15 cells, the expression of DN mutant was identified by immunofluorescence staining. The inhibitory effect of DN mutant on HBV replication was indexed as the supernatant HBsAg concentration determined by RIA and HBV-DNA content by fluorescent quantification-PCR (FQ-PCR). Meanwhile, metabolism of HepG2.2.25 cells was evaluated by MTI- colorimetry. RESULTS: VP22-based DN mutants and its truncated fragment were expressed in HepG2.2.15 cells, and had no toxic effect on host cells. DN mutants could inhibit HBV replication and the transduction ability of mutantbearing protein had a stronger inhibitory effect on HBV replication. DN mutants with full length of VP22 had the strongest inhibitory effect on HBV replication, reducing the HBsAg concentration by 81.94%, and the HBV-DNA content by 72.30%. MTT assay suggested that there were no significant differences in cell metabolic activity between the groups. CONCLUSION: VP22-based DN mutant can inhibit HBV replication effectively.展开更多
This article is concerned with the numerical investigation of one-dimensional population balance models for batch crystallization process with fines dissolution.In batch crystallization,dissolution of smaller unwanted...This article is concerned with the numerical investigation of one-dimensional population balance models for batch crystallization process with fines dissolution.In batch crystallization,dissolution of smaller unwanted nuclei below some critical size is of vital importance as it improves the quality of product.The crystal growth rates for both size-independent and size-dependent cases are considered.A delay in recycle pipe is also included in the model.The space–time conservation element and solution element method,originally derived for non-reacting flows,is used to solve the model.This scheme has already been applied to a range of PDEs,mainly in the area of fluid mechanics.The numerical results are compared with those obtained from the Koren scheme,showing that the proposed scheme is more efficient.展开更多
In the field of L2 (second language) reading, scholars generally agree that ER (extensive reading) improves L2 learners' reading speed and comprehension and enriches their L2 vocabulary (Grabe & Stoller, 1997)...In the field of L2 (second language) reading, scholars generally agree that ER (extensive reading) improves L2 learners' reading speed and comprehension and enriches their L2 vocabulary (Grabe & Stoller, 1997). This teacher-inquiry type of paper presents a practical suggestion for supplementing ER activity with the element of CL (cooperative learning). ER, theoretically speaking, focuses on the solitary task of silent reading. The CL technique used in this study was a book-talk activity. Forty-five freshmen from a course of children and young adult literatures were required to read at least 20 English books throughout a semester. CL was added to facilitate students' ER in young adult literature. After a semester, short-answer questions were asked regarding students' comments on ER as well as CL. Students overall agreed that when ER is supplemented with CL, reading in an L2 seems to be less intimidating.展开更多
In indigenous and contemporary societies, different elements of social and political institutions have come up with various inner mechanisms that are unique to their societies that can adjudicate in conflict situation...In indigenous and contemporary societies, different elements of social and political institutions have come up with various inner mechanisms that are unique to their societies that can adjudicate in conflict situations. In traditional, indigenous Yoruba societies, agba (elders) were usually relied upon as agents and institutions of conflict resolution in view of certain qualities possessed by this category of people. This paper focuses attention on the role of agba (elders) in conflict resolution at various levels of Yoruba life. We acknowledge that agba (elders) have performed these roles in traditional, indigenous Yoruba societies. The opinion expressed in this paper is that agba, as theoretical and practical conflict resolution mechanism, can be philosophically developed and used as an alternative model to the current means of resolving conflicts through wars and terrorism and other forms of violence by aggrieved individuals and groups in national and international arenas.展开更多
基金Financially supported by a grant from the Education Com-mittee of Hunan Province (No.ooAoo9)
文摘Objectives: To determine the susceptibilities of M.hominis and U. urealyticum to fluoroquinolones forthe instruction of reasonable clinical application ofantibiotics.Method: The susceptibilities of M. hominis and U.urealyticum to six fluoroquinolones were determinedby the broth dilution method.Results: Sparfloxacin and gatifloxacin were veryactive with MIC50S of 0.03125 and 0.25 μg/ml againstM. hominis, 0.25 and 0.5 μg/ml against U. urealyticum,respectively. Levofloxacin and ofloxacin had MIC50S of1 μg/ml and 2 μg/ml, respectively against both species.Norfloxacin was less effective against both species at16 and 32 μg/ml. Ciprofloxacin was unusual in thatthe MIC50S varied fourfold between species, with 2 μg/ml against M. hominis and 8 μg/ml against U.urealyticum.Conclusions: The results can provide useful infor-mation for selecting fluoroquinolones for treatmentof urogenital mycoplasma infections.
基金Projects (50821003,50405014) supported by the National Natural Science Foundation of ChinaProjects (10QH1401400,10520705000,10JC1407300) supported by Shanghai Committee of Science and Technology,China+1 种基金Project (NCET-07-0545) supported by Program for New Century Excellent Talents in University,ChinaProject supported by Ford University Research Program
文摘Two alternative formulations of single crystal plasticity model were introduced respectively and two schemes were implemented in the explicit FE code with software ABAQUS/Explicit by writing the user subroutine VUMAT.Meshes containing material data were created with solid elements.Each element represented an individual grain,and the grain orientations were explicitly stored and updated at each increment.Tangential modulus method was employed to calculate the plastic shear strain increment of deformation systems in combination with a hardening law to describe the hardening responses.Both two developed subroutines were applied to simulate the texture evolution during the uniaxial tension of copper(FCC),cold rolling of IF steel(BCC) and uniaxial compression of AZ31 magnesium alloy(HCP).The predicted texture distributions are in qualitative agreement with the experimental results.
文摘In this paper, we prove the existence in H+^2, an incomplete metric subspace of H^2×H^2×H^2, of global solutions to the system for a one-dimensional non-monotone fluid in bounded domainΩ=(0,1). The results in this paper have improved those previously related results.
基金Supported by the National Natural Science Foundation of China (No.20536020, No.20476033), the China Distinguished Young Scientist Fund (No.20225620) and Guangdong Province Science Fund (No.04020121).
文摘An emulsion-congealing technique is used to prepare solid lipid microparticles (SLM) containing ibuprofen with glyceryl behenate, tripalmitin and beewax as excipients. The difference of the solubility parameters between the excipients and ibuprofen are used to analyze their compatibility. Both the solubility parameter analysis and the experimental results show that glyceryl behenate is the best among the three excipients. The solid particles disperse well in aqueous phase when the drug loading reaches 10% (relative to lipid only). Glycerides exhibit marked polymorphism and their rapid rates of crystallization accelerate the formation of metastable crystal modification. The metastable crystal modification characterizes high drug loading capacity but less stability. Increasing the content of lipophilic drug in a lipid matrix facilitates the transformation of excipients to more stable polymorphic forms.
基金National Natural Science Foundation of China under Grant No.10371070the Special Found for Major Specialities of Shanghai Education CommitteeChina Postdoctoral Science Foundation
文摘Through the Wronskian technique, a simple and direct proof is presented that the AKNS hierarchy in the bilinear form has generalized double Wronskian solutions. Moreover, by using a unified way, soliton solutions, rational solutions, Matveev solutions and complexitons in double Wronskian form for it are constructed.
基金Sponsored by Fok Ying Tung Education Foundation (No. 94004)
文摘A combined first and second-order model, which includes bulk decay and wall decay, was developed to describe chlorine decay in water distribution systems. In the model the bulk decay has complex relationships with total organic carbon (TOC), the initial chlorine concentration and the temperature. Except for the initial stages they can be simplified into a linear increase with TOC, a linear decrease with initial chlorine concentration and an exponential relationship with the temperature. The model also explains why chlorine decays rapidly in the initial stages. The parameters of model are determined by deriving the best fitness with experimental data. And the accuracy of model has been verified by using the experimental data and the monitoring data in a distribution system.
基金Project(20010533009) supported by the Specialized Research Fund for the Doctoral Program of Higher Education of China
文摘Various busbar configurations were built and modeled by the custom code based on the commercial package ANSYS for the 500 kA aluminum electrolysis cell.The configuration parameters,such as side riser entry ratio,number of cathode bars connected to each riser,vertical location of side cathode busbar and short side cathode busbar,distance between rows of cells in potline,the number of neighboring cells,ratio of compensation busbar carried passing under cell and its horizontal location under cell along with large magnetohydrodynamic(MHD) computation based on the custom evaluation function were simulated and discussed.The results show that a cell with riser entry ratio of 11:9:8:9:11 and cathode busbar located at the level of aluminum solution,50% upstream cathode current passing under cell for magnetic field compensation,the distance between rows of 50 m is more stable.
文摘Hemopericardium is a common finding at autopsy, but it may represent a challenge for the forensic pathologist when the etiopathologi-cal relationship in causing death is requested. Hemopericardium and cardiac tamponade can be evaluated in living people using radiological techniques, in particular computer tomography (CT). Only a few studies are reported in literature involving post-mortem (PM) cases, where PMCT imaging has been used in order to investigate acute hemopericardium, and they have shown a good accuracy of this technique. Here we report a case involving a 70-year-old white male found dead on the beach, with a medical history of hepatitis C and chronic hypertension with a poor pharmacological response. A PMCT was performed about 3 h after the discovery of the body. The PMCT examination showed an intrapericardial aortic dissection associated to a periaortic hematoma, a sickle-shaped intramural hematoma, a false lumen, and a hemop-ericardium consisting in fluid and clotted blood. In this case, the PMCT was able to identify the cause of death, even though a traditional autopsy was required to confirm the radiological findings. PMCT is a reliable technique, which in chosen cases, can be performed without the need for a traditional autopsy to be carried out.
基金The National Natural Science Foundation of China (No 50471014)The Science and Technology Foundation of Shanghai (No0210nm017)
文摘A theoretical model on the solvus line prediction of a film was proposed and applied to a nanogranular A1-Cu system. The calculation results show that the solvus line of solute Cu will markedly lower with the decrease of grain size, namely, the starting temperature of θ (Al2Cu) precipitation in a nanogranular A1-Cu film will markedly lower than that of conventional coarse grain alloy with the same Cu concentration, and the precipitation temperatures calculated are comparable with the experimental ones. The theoretical model can be simply used to calculate the starting temperature of precipitation in A1-Cu films under three states: ① films with substrate; ②films without substrate; ③ultrafine grain bulk alloy. As a result, the model is universal, moreover, can be, in principle, used to predict precipitation temperature in other systems.
文摘This paper deals with the existence and nonexistence of global positive solutions of the following quasilinear parabolic equations:u t=1m△u m-u n, x∈Ω,t>0 1m·u mv=u p,x∈Ω,t>0 u(x,0)=u 0(x)>0,x∈Ω-where Ω∈R N is a bounded domain with smooth boundary Ω,m,n,p are positive constants, γ is the outward normal vector. The necessary and sufficient conditions for the global existence of solutions are obtained.
文摘Generation of induced pluripotent stem (iPS) cells from somatic cells has been achieved successfully by simultaneous viral transduction of defined reprogramming transcription factors (TFs). However, the process requires multiple viral vectors for gene delivery. As a result, generated iPS cells harbor numerous viral integration sites in their genomes. This can increase the probability of gene mutagenesis and genomic instability, and present significant barriers to both research and clinical application studies of iPS cells. In this paper, we present a simple lentivirus reprogramming system in which defined factors are fused in-frame into a single open reading frame (ORF) via self-cleaving 2A sequences. A GFP marker is placed downstream of the transgene to enable tracking of transgene expression. We demonstrate that this polycistronic expression system efficiently generates iPS cells. The generated iPS cells have normal karyotypes and are similar to mouse embryonic stem cells in morphology and gene expression. Moreover, they can differentiate into cell types of the three embryonic germ layers in both in vitro and in vivo assays. Remarkably, most of these iPS cells only harbor a single copy of viral vector. This system provides a valuable tool for generation of iPS cells, and our data suggest that the balance of expression of transduced reprogramming TFs in each cell is essential for the reprogramming process. More importantly, when delivered by non-integrating gene-delivery systems, this re-engineered single ORF will facilitate efficient generation of human iPS cells free of genetic modifications.
文摘TiO2 nanoparticles were prepared using the hydrothermal method and modified with CgN to syn-thesize a Type-Ⅱheterojunction semiconductor photocatalyst,TiO2-C;Na.In addition,a carbon layerwas coated onto the TiO2 nanoparticles and the obtained material was uniformly covered on thesurface of CaNa to form an all-solid-state Z-scheme semiconductor photocatalyst,TiO2-C-C3N4,Through characterization by XRD,XPS,SEM,TEM,BET,photoelectrochemical experiments,UV-visible diffuse reflection,and PL spectroscopy,the charge transfer mechanism and band gappositions for the composite photocatalysts were analyzed.The Type-Ⅱand all-solid-state Z-schemeheterojunction structures were compared.By combining microscopic internal mechanisms withmacroscopic experimental phenomena,the relationship between performance and structure wasverified.Experimental methods were used to explore the adaptation degree of different photocata-lytic mechanisms using the same degradation system.This study highlights effective photocatalystdesign to meet the requirements for specific degradation conditions.
基金Supported by the National Natural Science Foundation of China,No. 30400380
文摘AIM: To investigate the inhibitory effect of VP22 fusion protein-based dominant negative (DN) mutant on Hepatitis Bvrus (HBV) replication. METHODS: Full-length or truncated fragment of VP22 was fused to C terminal of HBV core protein (HBc), and subcloned into pcDNA3.1 (-) vector, yielding eukaryotic expression plasmids of DN mutant. After transfection into HepG2.2.15 cells, the expression of DN mutant was identified by immunofluorescence staining. The inhibitory effect of DN mutant on HBV replication was indexed as the supernatant HBsAg concentration determined by RIA and HBV-DNA content by fluorescent quantification-PCR (FQ-PCR). Meanwhile, metabolism of HepG2.2.25 cells was evaluated by MTI- colorimetry. RESULTS: VP22-based DN mutants and its truncated fragment were expressed in HepG2.2.15 cells, and had no toxic effect on host cells. DN mutants could inhibit HBV replication and the transduction ability of mutantbearing protein had a stronger inhibitory effect on HBV replication. DN mutants with full length of VP22 had the strongest inhibitory effect on HBV replication, reducing the HBsAg concentration by 81.94%, and the HBV-DNA content by 72.30%. MTT assay suggested that there were no significant differences in cell metabolic activity between the groups. CONCLUSION: VP22-based DN mutant can inhibit HBV replication effectively.
文摘This article is concerned with the numerical investigation of one-dimensional population balance models for batch crystallization process with fines dissolution.In batch crystallization,dissolution of smaller unwanted nuclei below some critical size is of vital importance as it improves the quality of product.The crystal growth rates for both size-independent and size-dependent cases are considered.A delay in recycle pipe is also included in the model.The space–time conservation element and solution element method,originally derived for non-reacting flows,is used to solve the model.This scheme has already been applied to a range of PDEs,mainly in the area of fluid mechanics.The numerical results are compared with those obtained from the Koren scheme,showing that the proposed scheme is more efficient.
文摘In the field of L2 (second language) reading, scholars generally agree that ER (extensive reading) improves L2 learners' reading speed and comprehension and enriches their L2 vocabulary (Grabe & Stoller, 1997). This teacher-inquiry type of paper presents a practical suggestion for supplementing ER activity with the element of CL (cooperative learning). ER, theoretically speaking, focuses on the solitary task of silent reading. The CL technique used in this study was a book-talk activity. Forty-five freshmen from a course of children and young adult literatures were required to read at least 20 English books throughout a semester. CL was added to facilitate students' ER in young adult literature. After a semester, short-answer questions were asked regarding students' comments on ER as well as CL. Students overall agreed that when ER is supplemented with CL, reading in an L2 seems to be less intimidating.
文摘In indigenous and contemporary societies, different elements of social and political institutions have come up with various inner mechanisms that are unique to their societies that can adjudicate in conflict situations. In traditional, indigenous Yoruba societies, agba (elders) were usually relied upon as agents and institutions of conflict resolution in view of certain qualities possessed by this category of people. This paper focuses attention on the role of agba (elders) in conflict resolution at various levels of Yoruba life. We acknowledge that agba (elders) have performed these roles in traditional, indigenous Yoruba societies. The opinion expressed in this paper is that agba, as theoretical and practical conflict resolution mechanism, can be philosophically developed and used as an alternative model to the current means of resolving conflicts through wars and terrorism and other forms of violence by aggrieved individuals and groups in national and international arenas.