高职院校中国传统文化类课程教学的困境与解径

中国传统文化底蕴深厚,源远流长,高职院校开展优秀传统文化教育,构建中国传统文化课程体系,对提高学生人文素养、培养文化自信,促进学生全面发展意义重大。当前,传统文化教育教学已纳入职业院校教育范畴,但因实际课时偏少、课程建设薄弱、师资力量不强、学生学习意愿低下等因素,致使课程开展面临困境。因此,科学选取教材,规范课程设置,加强师资建设,优化课程考核,对该课程真正肩负其教学任务、发挥其特有的德育价值效果显著。

Characterization of the dissociation pathways of dichloromethane and glutathione in dichloromethane dehalogenase

Dichloromethane(DCM)dehalogenase stands as a crucial enzyme implicated in the degradation of methylene chloride across diverse environmental and biological contexts.However,the unbinding pathways of ligands from DCM dehalogenase remain unexplored.In order to gain a deeper understanding of the binding sites and dissociation pathways of dichloromethane(DCM)and glutathione(GSH)from the DCM dehalogenase,random accelerated molecular dynamics(RAMD)simulations were performed,in which DCM and GSH were forced to leave the active site.The protein structure was predicted using Alphafold2,and the conformations of GSH and DCM in the binding pocket were predicted by docking.A long equilibrium simulation was conducted to validate the structure of the complex.The results show that GSH is most commonly observed in three main pathways,one of which is more important than the other two.In addition,DCM was observed to escape along a unique pathway.The key residues and protein helices of each pathway were identified.The results can provide a theoretical foundation for the subsequent dissociation mechanism of DCM dehalogenase.

基于利益均衡的高职院校教学改革激励机制探析

目前,高职院校的教学改革进行了许多有益的探索与实践,取得了很多成绩和经验;但在教学改革过程中也经历着反复的利益博弈,因利益冲突而产生阻抗,造成相关政策执行的扭曲,实施环节更是难以有效推进,教学改革面临一定的困境。借助利益均衡理论,对高职院校教学改革的困境及激励机制进行剖析,为教学改革的实质性突破提供新的视角和解径,具有一定的现实意义。

Dissociation Pathway Analysis of Thymine under Low Energy VUV Photon Excitation

Photon-induced dissociation pathways of thymine are investigated with vacuum ultraviolet photoionization mass spectrometry and theoretical calculations. The photoionization mass spectra of thymine at different photon energy are measured and presented. By selecting suitable photon energy, exclusively molecular ion m/z=126 is obtained. At photon energy of 12.0 eV, the major ionic fragments at m/z=98, 97, 84, 83, 70, and 55 are obtained, which are assigned to C4H6N2O＋, C4H5N2O＋, C3H4N2O＋ （or C4H6NO＋）, C4H5NO＋, C2NO2＋, and C3H5N＋, respectively. With help of theoretical calculations, the detailed dissociation pathways of thymine at low energy are well established.

Advances in Molecular Mechanism of Microbiological Degradation of Salicylate

Salicylate is a type of pollutant widely and persistently existed in environ- ment. Microbiological degradation of salicylate as one kind of biological remediation methods has the advantages of low cost, good effect and no secondary pollution, and also attracts wide attention both at home and abroad. Currently, many salicy- late-degrading bacteria are isolated and screened, while the molecular mechanism of salicylate metabolism is also worth deep research. The research about molecular mechanism of microorganism degradation was overviewed, various degrading path- ways including catechol meta-cleavage pathway and gentisate pathway were intro- duced, and the latest progress in regulating genes of salicylate degradation was summarized.

VUV Photoionization and Dissociation of Tyramine and Dopamine： the Joint Experimental and Theoretical Studies

Photon induced dissociation investigations of neutral tyramine and dopamine are carried out with synchrotron vacuum uRraviolet photoionization mass spectrometry and theoretical calculations. At low photon energy, only molecular ions are measured by virtue of nearthreshold photoionization. While increasing photon energy to 11.7 eV or more, four distinct fragment ions are obtained for tyramine and dopamine, respectively. Besides, the ionization energies of tyramine and dopamine are determined to be 7.984-0.05 and 7.674-0.05 eV by measuring the photoionization efficiency curves of corresponding molecular ions. With help of density function theory calculations, the detailed fragmentation pathways are established as well. These two molecular cations have similar aminoethyl group elimination pathways, CTHsO2＋ （m/z=124） and C7H8O＋ （m/z=108） are supposed to be generated by the McLafferty rearrangement via γ-hydrogen （7-H） shift inducing β-fission. And CH2NH2＋ is proposed to derive from the direct fission of C7-C8 bond. Besides, the McLafferty rearrangement and the C7-C8 bond fission are validated to be dominant dissociation pathways for tyramine and dopamine cations.

Overview of macroautophagy regulation in mammalian cells

Macroautophagy is a multistep, vacuolar, degradation pathway terminating in the lysosomal compartment, and it is of fundamental importance in tissue homeostasis. In this review, we consider macroautophagy in the light of recent advances in our understanding of the formation of autophagosomes, which are double-membrane-bound vacuoles that sequester cytoplasmic cargos and deliver them to lysosomes. In most cases, this final step is preceded by a maturation step during which autophagosomes interact with the endocytic pathway. The discovery of AuTophaGyrelated genes has greatly increased our knowledge about the mechanism responsible for antophagosome formation, and there has also been progress in the understanding of molecular aspects of autophagosome maturation. Finally, the regulation of autophagy is now better understood because of the discovery that the activity of Atg complexes is targeted by protein kinases, and owing to the importance of nuclear regulation via transcription factors in regulating the expression of autophagy genes.

Proteomic identification of potential target proteins regulated by an ASK1-mediated proteolysis pathway

The ASK1 （ARABIDOPSIS SKP1-LIKE） protein is a critical component of the SCF （Skpl-Cullin-F box protein） ubiquitin ligase complexes that recruit target proteins for degradation by the 26S proteosome. To investigate proteins that are affected by the ASK1-mediated proteolysis pathway in Arabidopsis flowers, we compared the proteomes of the Arabidopsis wild type and ask1 mutant flower buds using two-dimensional electrophoresis （2-DE）. Ten protein spots with higher or lower abundance in the ask1 mutant flowers compared to wild type flowers were excised and subjected to further mass spectrometry （MS） analysis. The results showed that they were proteins involved in photomorphogenesis, circadian oscillation, post-translation process, stress-responses and cell expansion or elongation, suggesting that those processes were affected in the ask1 mutant. The transcript levels of these genes were also compared based on the Affymetrix gene chip microarray data. No significant difference was observed for most of the genes, suggesting that the proteins with elevated levels of accumulation in the ask1 mutant could be candidate targets regulated by an ASK 1-mediated proteolysis pathway. These results help to elucidate the pleiotropic functions of ASK1 in Arabidopsis developmental processes and also demonstrate the importance and necessity of studying protein levels with respect to gene functions.

Life detection and location methods using UWB impulse radar in a coal mine

An ultra-wideband impulse radar was studied for the detection of buried life in coal mines. An improved Empirical Mode Decomposition (EMD) method based on a cross-correlation filter was proposed for reduction of multipath and noise interference. Multipath interference was first removed by cross-corre- lation filtering. Then the delays of each pulse in every echo were summed. An EMD algorithm was used for noise reduction for the total delay of each echo. The corresponding EMD results of every echo were then summed and averaged. Finally, evidence for the existence of buried life and their position were obtained from amplitude-frequency curves of the averaged EMD results. Detailed simulation experi- ments are presented to validate the effectiveness of this proposed method. The experimental results show that this method can efficiently eliminate multipath interference and reduce noise interference in echoes, which makes detection and location of buried life in coal mines more accurate.

An Enhanced NEH Method in Solving Permutation Flow Shop Problem

This paper proposed an enhanced NEH with full insertion moves to solve the permutation flow shop problem.The characteristics of the original NEH are investigated and analyzed,and it is concluded that the given method would be promising to find better solutions,while the cost would be increased.Fast makespan calculating method and eliminating non-promising permutation policy are introduced to reduce the evaluation effort.The former decreases the time complexity from O(n4m) to O(n3m),which is an acceptable cost for medium and small size instances considering the obtained solution quality.The results from computational experience show that the latter also can eliminate a lot of non-promising solutions.

Effect of composition and aging time on hardness and wear behavior of Cu-Ni-Sn spinodal alloy

Copper alloyed with various compositions of nickel and tin were cast into molds under argon atmosphere.The cast rods were homogenized,solution heat treated,followed by aging for different time duration.The specimens were characterized for microstructure and tested for microhardness and wear rate.A hybrid model with a linear function and radial basis function was developed to analyze the influence of nickel,tin,and aging time on the microhardness and tribological behavior of copper-nickel-sin alloy system.The results indicate that increase in the composition of nickel and tin increases the microhardness and decreases the wear rate of the alloy.The increase in the concentration of nickel and tin decreases the peak aging time of the alloy system.

Exact Two-Soliton Solutions for Discrete mKdV Equation

An exact two-soliton solution of discrete mKdv equation is derived by using the Hirota direct approach. In addition, we plot the soliton solutions to discuss the properties of solitons. It is worth while noting that we obtain the completely elastic interaction between the two solitons.

Exact Solutions of the φ^6 ＋ φ^5 Model Using Truncated Method

In this letter, the φ^6 ＋ φ^5 model in （D ＋ 1） dimensions can be solved by a truncated series method. A series of solitary solutions of the φ^6 ＋ φ^5 model in （D ＋ 1） dimensions have be obtained.

Degradation of p-nitrotoluene by O_3/H_2O_2 process and oxidation mechanism

The degradation of p-nitrotoluene by O3/H2O2 process in a bubble contact column was investigated. Effects of the molar ratio of hydrogen peroxide to ozone,pH value and t-butanol on the oxidation process were discussed. It was found that the proper H2O2/O3 molar ratio for the degradation of p-nitrotoluene was around 0.6, different pH values and the presence of t-butanol highly influenced the removal efficiency of p-nitrotoluene. 5-methyl-2-nitrophenol, 2-methyl-5-nitrophenol, (4-nitrophenyl) methanol, 5-(hydroxymethyl)-2-nitro phenol, acetic acid, 2-methylpropane diacid and 2-(hydroxylmethyl)propane diacid were identified as degradation intermediates and products through GC-MS. Radical reaction mechanism and degradation pathway were proposed based on the results of experiments. It is deduced that the benzene ring of p-nitrotoluene can be only destroyed by hydroxyl radicals through a polyhydroxy intermediate pathway. Then unstable polyhydroxy intermediates can be oxidized to different acids with low molecular weight rapidly.

Modeling for mean ion activity coefficient of strong electrolyte system with new boundary conditions and ion-size parameters

A rigorous approach is proposed to model the mean ion activity coefficient for strong electrolyte systems using the Poisson-Boltzmann equation. An effective screening radius similar to the Debye decay length is introduced to define the local composition and new boundary conditions for the central ion. The crystallographic ion size is also considered in the activity coefficient expressions derived and non-electrostatic contributions are neglected. The model is presented for aqueous strong electrolytes and compared with the classical Debye-Hfickel （DH） limiting law for dilute solutions. The radial distribution function is compared with the DH and Monte Carlo studies. The mean ion activity coefficients are calculated for 1：1 aqueous solutions containing strong electrolytes composed of alkali halides. The individual ion activity coefficients and mean ion activity coefficients in mixed sol- vents are predicted with the new equations.

Hooke's Atom in an Arbitrary External Electric Field: Analytical Solutions of Two-Electron Problem by Path Integral Approach

By using the path integral approach, we investigate the problem of Hooke＇s atom （two electrons interacting with Coulomb potential in an external harmonic-oscillator potential） in an arbitrary time-dependent electric field. For a certain infinite set of discrete oscillator frequencies, we obtain the analytical solutions. The ground state polarization of the atom is then calculated. The same result is also obtained through linear response theory.

Solutions of Laplace Equation in n-Dimensional Spaces

The solutions of the Laplace equation in n-dimensional space are studied. The angular eigenfunctions have the form of associated Jacob/polynomials. The radial solution of the Helmholtz equation is derived.

INVESTIGATION OF THE INTERACTIONS BETWEEN WATER AND MODIFIED SILICA GEL BY IGC AND TPD

In this work, the thermodynamic parameters for the adsorption of water vapor on untreated silica gel and silica gel treated with hygroscopic salts and silane coupling agent were determined by lnverse Gas Chromatography （IGC） in the infinite dilution region. The desorption activation energies of the water vapor on virgin and modified silica gels were estimated by using the Temperature Programmed Desorption （TPD） technique. The interactions between the water and the virgin and modified silica gels were discussed. Results showed that the thermodynamic parameters and desorption activation energy of water vapour on the silica gels increase with decreasing pore size and increasing the surface hydrophilic properties. The desorption activation energy of virgin and modified silica gels was found to increase with increasing the thermodynamic parameters. The larger the adsorption parameters and the desorption activation energy were, the interactions between water and virgin and modified silica gels were.

Path planning of the robot assembly based on Voronoi diagram

Based on the concepts of Voronoi diagram that describes geometry information of the robot assembly in C space, the position vector path parameter equation of the assembly movement between the step shaft and two-sided bearing bracket was given. And the path planning strategy of the component initiative assembly was put forward as well. Theoretical analysis proves that using the Voronoi diagram to do the geometry reasoning on the assembly space can evaluate the feasibility of the component assembly, and can present the reference position vector path of the component movement from the initial configuration to the objective configuration, therefore improves the flexibility of the robot initiative assembly.

Some Properties of Two Subclasses of Analytic Functions

This paper discusses some properties of two classes Vk[α,β] and Rk[α,β ]. Sharp distortion theorem and radius of convexity and starlikeness are obtained. Hadamard product of functions in the classes are also studied.