The transformation of an anthraquinone dye blue 324 in a facultative-aerobic(F-A) system was investigated.Kinetic parameter study showed that higher Vmax coupled with more recalcitrant chemical oxygen demand(COD) were...The transformation of an anthraquinone dye blue 324 in a facultative-aerobic(F-A) system was investigated.Kinetic parameter study showed that higher Vmax coupled with more recalcitrant chemical oxygen demand(COD) were found in the facultative biofilm reactor(FBR) than in the aerobic reactor(AR).Results of the product analyses indicated that most of dye molecular could be facultatively broken down into simple intermediates,which would be further degraded under subsequent aerobic condition.The main metabolites in each reactor were detected by infrared(FT-IR) and high performance liquid chromatography and mass spectrometry(HPLC-MS).Comparison of the toxicities among the dye and its metabolites was conducted,surprisingly,the colorless intermediates from FBR possessed less inhibitory than original dye and the median effective luminescence concentration(EC50) in 15 min for aerobic effluent could not be detected,showing that hardly toxic products existed in the aerobic process effluent.展开更多
The pyrolysis of isopsoralen was studied by synchrotron vacuum ultraviolet photoionization mass spectrometry at low pressure. The pyrolysis products were detected at different photon energies, the ratios of products t...The pyrolysis of isopsoralen was studied by synchrotron vacuum ultraviolet photoionization mass spectrometry at low pressure. The pyrolysis products were detected at different photon energies, the ratios of products to precursor were measured at various pyrolysis temperatures. The experimental results demonstrate that the main pyrolysis products are primary CO and sequential CO elimination products （C10H602 and C9H60）. The decomposition channels of isopsoralen were also studied by the density functional theory, then rate constants for competing pathways were calculated by the transition state theory. The dominant decom- position channels of isopsoralen and the molecular structures for corresponding products were identified by combined experimental and theoretical studies.展开更多
Objective:In the medium of ethanol(non-polar),STMP and ADH as crosslinkers to HA,to prepare drug delivery microspheres by getting stable cross-linked products without a gel phase,and to study biocompatibility and biod...Objective:In the medium of ethanol(non-polar),STMP and ADH as crosslinkers to HA,to prepare drug delivery microspheres by getting stable cross-linked products without a gel phase,and to study biocompatibility and biodegradability of cross-linked products.Methods:ISO10993.1-Safety Evaluation of Biomedical materials as a reference,to make hemolysis test,percutaneous stimulation test,acute toxicity test,and analyze in vitro degradation test,and degradation products.Results:HA-STMP cross-linked product had no hemolysis,no irritation,no acute systemic toxicity,but HA-ADH had a mild skin irritation and adverse acute systemic toxicity.HA-STMP cross-linked product had lower sensitivity on HAse and the curve is flatting.With the increase of degradation time HA-STMP results were changed by the structure analysis,degradation products of these cells were no toxicity.Conclusion:HA-STMP cross-linked products with better biocompatibility and better resistance to hydrolysis could delay the degradation time,which is suitable for the preparation of biodegradable drug carriers.展开更多
基金Natural Science Foundation of Shanghai,China (No.06ZR14002)Shanghai Leading Academic Discipline Project (No.B604)
文摘The transformation of an anthraquinone dye blue 324 in a facultative-aerobic(F-A) system was investigated.Kinetic parameter study showed that higher Vmax coupled with more recalcitrant chemical oxygen demand(COD) were found in the facultative biofilm reactor(FBR) than in the aerobic reactor(AR).Results of the product analyses indicated that most of dye molecular could be facultatively broken down into simple intermediates,which would be further degraded under subsequent aerobic condition.The main metabolites in each reactor were detected by infrared(FT-IR) and high performance liquid chromatography and mass spectrometry(HPLC-MS).Comparison of the toxicities among the dye and its metabolites was conducted,surprisingly,the colorless intermediates from FBR possessed less inhibitory than original dye and the median effective luminescence concentration(EC50) in 15 min for aerobic effluent could not be detected,showing that hardly toxic products existed in the aerobic process effluent.
文摘The pyrolysis of isopsoralen was studied by synchrotron vacuum ultraviolet photoionization mass spectrometry at low pressure. The pyrolysis products were detected at different photon energies, the ratios of products to precursor were measured at various pyrolysis temperatures. The experimental results demonstrate that the main pyrolysis products are primary CO and sequential CO elimination products （C10H602 and C9H60）. The decomposition channels of isopsoralen were also studied by the density functional theory, then rate constants for competing pathways were calculated by the transition state theory. The dominant decom- position channels of isopsoralen and the molecular structures for corresponding products were identified by combined experimental and theoretical studies.
文摘Objective:In the medium of ethanol(non-polar),STMP and ADH as crosslinkers to HA,to prepare drug delivery microspheres by getting stable cross-linked products without a gel phase,and to study biocompatibility and biodegradability of cross-linked products.Methods:ISO10993.1-Safety Evaluation of Biomedical materials as a reference,to make hemolysis test,percutaneous stimulation test,acute toxicity test,and analyze in vitro degradation test,and degradation products.Results:HA-STMP cross-linked product had no hemolysis,no irritation,no acute systemic toxicity,but HA-ADH had a mild skin irritation and adverse acute systemic toxicity.HA-STMP cross-linked product had lower sensitivity on HAse and the curve is flatting.With the increase of degradation time HA-STMP results were changed by the structure analysis,degradation products of these cells were no toxicity.Conclusion:HA-STMP cross-linked products with better biocompatibility and better resistance to hydrolysis could delay the degradation time,which is suitable for the preparation of biodegradable drug carriers.
