Purpose： To extract protein, decrease the cellulose and facilitate the digestion and absorption of brewers＇ spent grain by animal. Topic： Discuss and optimize the hydrolysis conditions of the combined enzymatic hyd...Purpose： To extract protein, decrease the cellulose and facilitate the digestion and absorption of brewers＇ spent grain by animal. Topic： Discuss and optimize the hydrolysis conditions of the combined enzymatic hydrolysis by Novozymes. Method： The fresh brewers＇ spent grain was firstly dried, smashed and sifted. Then as indicators of the protein extraction rate in the enzyme solution and the content of cellulose in the index, the parameters of enzymatic hydrolysis, such as the solid-liquid ratio, reaction temperature, pH, enzyme dosage and reaction time, were investigated in detailed. After hydrolysis, the brewers＇ spent grain was put in the boiling water bath for inactivation for 15 minutes, and centrifuged, the supernatants were volume to 100 mL and the protein content was measured. After the precipitate was dried, the cellulose content was also measured. Achievements： The optimized conditions were with temperature of 50 ℃, pH 6.5, enzyme amount of 30 mg for Novozymes enzyme and 2.5 h for reaction time. Under these conditions, the protein extraction rate in the enzyme reaction reached 41.82%, and the cellulose content reached 13.90%, the degradation rate of cellulose was 18.86%.展开更多
文摘Purpose： To extract protein, decrease the cellulose and facilitate the digestion and absorption of brewers＇ spent grain by animal. Topic： Discuss and optimize the hydrolysis conditions of the combined enzymatic hydrolysis by Novozymes. Method： The fresh brewers＇ spent grain was firstly dried, smashed and sifted. Then as indicators of the protein extraction rate in the enzyme solution and the content of cellulose in the index, the parameters of enzymatic hydrolysis, such as the solid-liquid ratio, reaction temperature, pH, enzyme dosage and reaction time, were investigated in detailed. After hydrolysis, the brewers＇ spent grain was put in the boiling water bath for inactivation for 15 minutes, and centrifuged, the supernatants were volume to 100 mL and the protein content was measured. After the precipitate was dried, the cellulose content was also measured. Achievements： The optimized conditions were with temperature of 50 ℃, pH 6.5, enzyme amount of 30 mg for Novozymes enzyme and 2.5 h for reaction time. Under these conditions, the protein extraction rate in the enzyme reaction reached 41.82%, and the cellulose content reached 13.90%, the degradation rate of cellulose was 18.86%.
