Objective To observe the influence of rotenone on the distribution of α-synuclein （ASN） in rat model of Parkinson＇s disease （PD）. Methods Wistar rats were randomly divided into two groups and received 2 mg/kg ro...Objective To observe the influence of rotenone on the distribution of α-synuclein （ASN） in rat model of Parkinson＇s disease （PD）. Methods Wistar rats were randomly divided into two groups and received 2 mg/kg rotenone （s.c.） or sunflower oil （as control group） for about 4 weeks. The hippocampus, substantia nigra and striatum of brain were observed. Hematoxylin and eosin stain were used to observe the Lewy body like inclusion. The expression of tyrosine hydroxylase （TH） or ASN protein was determined by anti-TH or anti-α-synuclein immunohistochemistry, respectively. Results In control rats, ASN protein distributed widely in brain, especially in hippocampus, cortex and striatum. Rotenone obviously increased TH positive neurons and fibers loss in substantia nigra and striatum （P 〈 0.05）. In rotenone treated rats, ASN positive cells increased in global brain but not distributed in an even manner. In substantia nigra, ASN positive stuff was found aggregate in both cytoplasm and nucleus, and some formed spherical inclusion; in striatum, ASN positive neurites end aggregated and agglomerated around neurons; and in hippocampus, few dot-like ASN were aggregated in cell body, and no notable change was found in nucleus. Conclusion In rotenone administrated PD rats, ASN protein aggregated in several brain regions but most obviously in striatum and substantia nigra, and the distribution region of ASN was changed from peri-synapse to the cytoplasm and nucleus of dopaminergic neuron.展开更多
The toxicities of 4 common endocrine-disrupting chemicals （EDCs）, 17β-estradiol （E2）, p,p＇-dichlorodiphenyldi- chloro-ethylene （DDE）, 4-nonylphenol （NP） and tributyltin （TBT）, to sperm motility, fertilizat...The toxicities of 4 common endocrine-disrupting chemicals （EDCs）, 17β-estradiol （E2）, p,p＇-dichlorodiphenyldi- chloro-ethylene （DDE）, 4-nonylphenol （NP） and tributyltin （TBT）, to sperm motility, fertilization rate, hatching rate and embryonic development of Barbel chub （Squaliobarbus curriculus） were investigated in this study. The duration of sperm motility was signifi- cantly shortened by exposure to the EDCs at the threshold concentrations of 10ngL-1 for E2 and TBT, 1 μgL l for NP and 100 μgL-1 for DDE, respectively. The fertilization rate was substantially reduced by the EDCs at the lowest observable effect concentrations （LOECs） of 10 ngL-1 for E2 and TBT and 10μgL-1 for DDE and NP, respectively. Of the tested properties of S. curriculus, larval deformity rate was most sensitive to EDC exposure and was significantly increased by DDE at the lowest experimental level of 0.1 μgL-1. Other EDCs increased the larval deformity rate at the LOECs of 1 ngL-1 for E2, 10ngL-1 for TBT and 1μgL-1 for NP, re- spectively. Despite their decreases with the increasing EDC concentrations, the hatching rate and larval survival rate of S. curriculus were not significantly affected by the exposure to EDCs. The results indicated that all the 4 EDCs affected significantly and nega- tively the early life stages of the freshwater fish S. curriculus. Overall, E2 and TBT were more toxic than NP and DDE, while DDE might be more toxic to larval deformity rate than to other measured parameters. Thus, the 4 EDCs showed potential negative influ- ences on natural population dynamics of S. curriculus. Our findings provided valuable basic data for the ecological risk assessment of E2, DDE, NP and TBT.展开更多
A new species of cymothoid Isopoda, Nerocila donghaiensis , collected from the body of a sciaenid fish, Johnius sp., is described in the present paper. The new species can be distinguished from the other species of ge...A new species of cymothoid Isopoda, Nerocila donghaiensis , collected from the body of a sciaenid fish, Johnius sp., is described in the present paper. The new species can be distinguished from the other species of genus Nerocila by its endopod of uropods with deep notch, sharply serrated on the lateral margin and strongly serrated on the medial margin; antennule with 1st article swollen; dactyli of pereopods I, II, IV, V swollen.展开更多
基金supported by the National Nature Science Foundation of China(No.30570627).
文摘Objective To observe the influence of rotenone on the distribution of α-synuclein （ASN） in rat model of Parkinson＇s disease （PD）. Methods Wistar rats were randomly divided into two groups and received 2 mg/kg rotenone （s.c.） or sunflower oil （as control group） for about 4 weeks. The hippocampus, substantia nigra and striatum of brain were observed. Hematoxylin and eosin stain were used to observe the Lewy body like inclusion. The expression of tyrosine hydroxylase （TH） or ASN protein was determined by anti-TH or anti-α-synuclein immunohistochemistry, respectively. Results In control rats, ASN protein distributed widely in brain, especially in hippocampus, cortex and striatum. Rotenone obviously increased TH positive neurons and fibers loss in substantia nigra and striatum （P 〈 0.05）. In rotenone treated rats, ASN positive cells increased in global brain but not distributed in an even manner. In substantia nigra, ASN positive stuff was found aggregate in both cytoplasm and nucleus, and some formed spherical inclusion; in striatum, ASN positive neurites end aggregated and agglomerated around neurons; and in hippocampus, few dot-like ASN were aggregated in cell body, and no notable change was found in nucleus. Conclusion In rotenone administrated PD rats, ASN protein aggregated in several brain regions but most obviously in striatum and substantia nigra, and the distribution region of ASN was changed from peri-synapse to the cytoplasm and nucleus of dopaminergic neuron.
基金supported by the National Science Foundation of China(No.40632009)
文摘The toxicities of 4 common endocrine-disrupting chemicals （EDCs）, 17β-estradiol （E2）, p,p＇-dichlorodiphenyldi- chloro-ethylene （DDE）, 4-nonylphenol （NP） and tributyltin （TBT）, to sperm motility, fertilization rate, hatching rate and embryonic development of Barbel chub （Squaliobarbus curriculus） were investigated in this study. The duration of sperm motility was signifi- cantly shortened by exposure to the EDCs at the threshold concentrations of 10ngL-1 for E2 and TBT, 1 μgL l for NP and 100 μgL-1 for DDE, respectively. The fertilization rate was substantially reduced by the EDCs at the lowest observable effect concentrations （LOECs） of 10 ngL-1 for E2 and TBT and 10μgL-1 for DDE and NP, respectively. Of the tested properties of S. curriculus, larval deformity rate was most sensitive to EDC exposure and was significantly increased by DDE at the lowest experimental level of 0.1 μgL-1. Other EDCs increased the larval deformity rate at the LOECs of 1 ngL-1 for E2, 10ngL-1 for TBT and 1μgL-1 for NP, re- spectively. Despite their decreases with the increasing EDC concentrations, the hatching rate and larval survival rate of S. curriculus were not significantly affected by the exposure to EDCs. The results indicated that all the 4 EDCs affected significantly and nega- tively the early life stages of the freshwater fish S. curriculus. Overall, E2 and TBT were more toxic than NP and DDE, while DDE might be more toxic to larval deformity rate than to other measured parameters. Thus, the 4 EDCs showed potential negative influ- ences on natural population dynamics of S. curriculus. Our findings provided valuable basic data for the ecological risk assessment of E2, DDE, NP and TBT.
文摘A new species of cymothoid Isopoda, Nerocila donghaiensis , collected from the body of a sciaenid fish, Johnius sp., is described in the present paper. The new species can be distinguished from the other species of genus Nerocila by its endopod of uropods with deep notch, sharply serrated on the lateral margin and strongly serrated on the medial margin; antennule with 1st article swollen; dactyli of pereopods I, II, IV, V swollen.
