This paper discusses some issues on human reliability model of time dependent human behavior. Some results of the crew reliability experiment on Tsinghua training simulator in China are given, Meanwhile, a case of ca...This paper discusses some issues on human reliability model of time dependent human behavior. Some results of the crew reliability experiment on Tsinghua training simulator in China are given, Meanwhile, a case of calculation for human error probability during anticipated transient without scram (ATWS) based on the data drew from the recent experiment is offered.展开更多
Objective To investigate the effectiveness of compound Chai Jin Jie Yu Tablets(CJJYT)in ameliorating cognitive impairment associated with depression and its potential mechanism of action.Methods In vitro experiments,t...Objective To investigate the effectiveness of compound Chai Jin Jie Yu Tablets(CJJYT)in ameliorating cognitive impairment associated with depression and its potential mechanism of action.Methods In vitro experiments,the hippocampus was isolated from the whole brain of the fetal rat and cultured into hippocampal neuron cells.50μM corticosterone(CORT)was added to each group 18 h before the experiment for modeling depression,with the exception of the control group.After modeling,the blank serum group was added with 10%blank serum,the CJJYT group and the venlafaxine group were added with the corresponding 10%drug-containing serum,and the control group and the model group were added with equal volumes of culture medium.The intracellular Ca^2+mean fluorescence intensity,miniature excitatory postsynaptic current(mEPSC)current amplitude,and frequency of different hippocampal neurons were evaluated as indicators of synaptic function in the hippocampal neurons.In addition,the expression of synaptic plasticity related proteins,synaptophysin-α(SYN-α),N-methyl-D-aspartate receptor 2A(NR2A),N-methyl-Daspartate receptor 2B(NR2B),post synaptic density 95 protein(PSD-95),calcium/calmodulin dependent protein kinaseⅡ(CaMKⅡ)and synaptic associated protein(SynGAP)were detected in the hippocampal neurons by immunofluorescence staining and high content analysis(HCA)system.Then,reverse transcription-polymerase chain reaction(RT-PCR)was used to detect the mRNA expression levels of SYN-α,NR2A,NR2B,PSD-95,CaMKⅡand SynGAP.For in vivo experiments,except for those in the blank control group,all rats were treated within a single cage for chronic unpredictable stress-induced depression modeling and subjected to corresponding drug interventions.Behavioral tests were used to detect depressive behavior and determine learning,memory and other cognitive abilities,whereas enzyme-linked immunosorbent assay(ELISA)was used to detect the CORT levels.Golgi-Cox staining was used to observe changes in the synaptic morphology of parahippocampal gyrus CA1 area(CA1)and dentategyrus(DG).Results In vitro,CJJYT treatment reduced the intracellular Ca^2+mean flurorescence intensity in the hippocampal neurons(P<0.05),causing a reduction in the frequency and current amplitude of mEPSC(P<0.05),and thus inhibited the excessive activation of post-synaptic receptors.CJJYT treatment reduced the protein and mRNA expression of SYN-α,NR2A,NR2B and PSD-95 in the hippocampal neurons(P<0.05),increased the mRNA and protein expression of CaMKⅡand SynGAP(P<0.05),and thereby improved the synaptic plasticity of the hippocampal neurons.In vivo,CJJYT intervention improved sucrose preference,voluntary activity,learning and memory ability of Morris water maze test,and suppressed appetite(P<0.05),and increased the despair feeling of forced swimming test(P<0.05).The CORT level was reduced(P<0.05),leading to the repair of synaptic damage in the hippocampal neurons.Conclusions CJJYT can improve the synaptic function of hippocampal neurons and has obvious protective effects on neurons.It can repair the structural damage in the hippocampal neurons,improving the cognitive ability of the depressed model rats.The mechanism of CJJYT improving cognition in depressed rats may be related to the transmission and function of SYN-α/NR and its downstream neurotransmitters.展开更多
Shipboard operations as a hazardous process require the human element to be aware of any operation risks. Since the concept of human error exposed to ample arguments, the introduction of human element concerns to prac...Shipboard operations as a hazardous process require the human element to be aware of any operation risks. Since the concept of human error exposed to ample arguments, the introduction of human element concerns to practices rather than other means is essential because the provenance of accidents is human error. HRA （human reliability assessment） is a theoretical framework to assess the human actions for predicting the potential human error probability of a certain given task or operation scenario. Furthermore, surveillance of the human performance is through the task by steps and sub-steps. The CREAM （cognitive reliability and error analysis method） tool is the second generation of HRA which emphasizes the features of the context and utilized as retrospective and prospective tool. The paper illustrates the basic and extended version of CREAM and its suitability for critical shipboard operations safety assessment.展开更多
Kuhn＇s incommensurability-thesis is crucial for consequences for the pursuit of epistemology. A interpreting his views on the development of science and their commemoration of the 50th anniversary of the publication ...Kuhn＇s incommensurability-thesis is crucial for consequences for the pursuit of epistemology. A interpreting his views on the development of science and their commemoration of the 50th anniversary of the publication of the original version of Kuhn＇s epoch-making book The Structure of Scientific Revolutions （henceforth： SSR） should thus provide a thorough reflection on this thesis. However, this thesis is not easy to interpret. It is not only complex in itself but has also undergone a historical development--in Kuhn＇s own hands and in those of his interpreters. In this article, I sort out the different interpretations of it, in particular, in Part A. In Part B, I demonstrate their epistemological consequences. Under closer scrutiny, Kuhn＇s incommensurability-thesis contains several sub-theses Different senses of ＂incommensurability＂ thus need to be distinguished. However, the way in which those distinctions are drawn in Kuhn-scholarship differs. In paragraph I of Part A, I provide an overview of the reception of the incommensurability-thesis in Kuhn-scholarship. In Paragraph II, I trace its development in Kuhn＇s later writings： given its importance and contested nature, Kuhn later clarifies his original thesis. Those later clarifications＇ main function consists in domesticating the most radically relativistic aspects his original incommensurability-thesis had, at least, in the eyes of his interpreters. The upshot of Part A （Paragraphs I and II） is to provide a coherent interpretation of Kuhn＇s incommensurability-thesis. To that end, I distinguish in line with much of Kuhn-scholarship a semantic from a methodological sense of incommensurability. In part B, the question is raised： What sort of epistemological consequences follow from both senses of incommensurability？ In particular, what consequences follow for the issues of reference, subjectivity （objectivity）, pluralism, and realism？ The underlying question is to what extent Kuhnian incommensurability caters to a relativistic understanding of those issues. This question is answered in Paragraph Ill with the help of the analyses of a currently leading Kuhn-scholar, C. H. Sankey. His answers are taken as a vantage point for my concluding evaluation of the consequences of Kuhnian incommensurability in Paragraph IV.展开更多
文摘This paper discusses some issues on human reliability model of time dependent human behavior. Some results of the crew reliability experiment on Tsinghua training simulator in China are given, Meanwhile, a case of calculation for human error probability during anticipated transient without scram (ATWS) based on the data drew from the recent experiment is offered.
基金funding support from the National Major New Drug Development Project(No.2017ZX09309026)Provincial Department of Graduate Research Innovation Project of Hunan(No.CX20190565)。
文摘Objective To investigate the effectiveness of compound Chai Jin Jie Yu Tablets(CJJYT)in ameliorating cognitive impairment associated with depression and its potential mechanism of action.Methods In vitro experiments,the hippocampus was isolated from the whole brain of the fetal rat and cultured into hippocampal neuron cells.50μM corticosterone(CORT)was added to each group 18 h before the experiment for modeling depression,with the exception of the control group.After modeling,the blank serum group was added with 10%blank serum,the CJJYT group and the venlafaxine group were added with the corresponding 10%drug-containing serum,and the control group and the model group were added with equal volumes of culture medium.The intracellular Ca^2+mean fluorescence intensity,miniature excitatory postsynaptic current(mEPSC)current amplitude,and frequency of different hippocampal neurons were evaluated as indicators of synaptic function in the hippocampal neurons.In addition,the expression of synaptic plasticity related proteins,synaptophysin-α(SYN-α),N-methyl-D-aspartate receptor 2A(NR2A),N-methyl-Daspartate receptor 2B(NR2B),post synaptic density 95 protein(PSD-95),calcium/calmodulin dependent protein kinaseⅡ(CaMKⅡ)and synaptic associated protein(SynGAP)were detected in the hippocampal neurons by immunofluorescence staining and high content analysis(HCA)system.Then,reverse transcription-polymerase chain reaction(RT-PCR)was used to detect the mRNA expression levels of SYN-α,NR2A,NR2B,PSD-95,CaMKⅡand SynGAP.For in vivo experiments,except for those in the blank control group,all rats were treated within a single cage for chronic unpredictable stress-induced depression modeling and subjected to corresponding drug interventions.Behavioral tests were used to detect depressive behavior and determine learning,memory and other cognitive abilities,whereas enzyme-linked immunosorbent assay(ELISA)was used to detect the CORT levels.Golgi-Cox staining was used to observe changes in the synaptic morphology of parahippocampal gyrus CA1 area(CA1)and dentategyrus(DG).Results In vitro,CJJYT treatment reduced the intracellular Ca^2+mean flurorescence intensity in the hippocampal neurons(P<0.05),causing a reduction in the frequency and current amplitude of mEPSC(P<0.05),and thus inhibited the excessive activation of post-synaptic receptors.CJJYT treatment reduced the protein and mRNA expression of SYN-α,NR2A,NR2B and PSD-95 in the hippocampal neurons(P<0.05),increased the mRNA and protein expression of CaMKⅡand SynGAP(P<0.05),and thereby improved the synaptic plasticity of the hippocampal neurons.In vivo,CJJYT intervention improved sucrose preference,voluntary activity,learning and memory ability of Morris water maze test,and suppressed appetite(P<0.05),and increased the despair feeling of forced swimming test(P<0.05).The CORT level was reduced(P<0.05),leading to the repair of synaptic damage in the hippocampal neurons.Conclusions CJJYT can improve the synaptic function of hippocampal neurons and has obvious protective effects on neurons.It can repair the structural damage in the hippocampal neurons,improving the cognitive ability of the depressed model rats.The mechanism of CJJYT improving cognition in depressed rats may be related to the transmission and function of SYN-α/NR and its downstream neurotransmitters.
文摘Shipboard operations as a hazardous process require the human element to be aware of any operation risks. Since the concept of human error exposed to ample arguments, the introduction of human element concerns to practices rather than other means is essential because the provenance of accidents is human error. HRA （human reliability assessment） is a theoretical framework to assess the human actions for predicting the potential human error probability of a certain given task or operation scenario. Furthermore, surveillance of the human performance is through the task by steps and sub-steps. The CREAM （cognitive reliability and error analysis method） tool is the second generation of HRA which emphasizes the features of the context and utilized as retrospective and prospective tool. The paper illustrates the basic and extended version of CREAM and its suitability for critical shipboard operations safety assessment.
文摘Kuhn＇s incommensurability-thesis is crucial for consequences for the pursuit of epistemology. A interpreting his views on the development of science and their commemoration of the 50th anniversary of the publication of the original version of Kuhn＇s epoch-making book The Structure of Scientific Revolutions （henceforth： SSR） should thus provide a thorough reflection on this thesis. However, this thesis is not easy to interpret. It is not only complex in itself but has also undergone a historical development--in Kuhn＇s own hands and in those of his interpreters. In this article, I sort out the different interpretations of it, in particular, in Part A. In Part B, I demonstrate their epistemological consequences. Under closer scrutiny, Kuhn＇s incommensurability-thesis contains several sub-theses Different senses of ＂incommensurability＂ thus need to be distinguished. However, the way in which those distinctions are drawn in Kuhn-scholarship differs. In paragraph I of Part A, I provide an overview of the reception of the incommensurability-thesis in Kuhn-scholarship. In Paragraph II, I trace its development in Kuhn＇s later writings： given its importance and contested nature, Kuhn later clarifies his original thesis. Those later clarifications＇ main function consists in domesticating the most radically relativistic aspects his original incommensurability-thesis had, at least, in the eyes of his interpreters. The upshot of Part A （Paragraphs I and II） is to provide a coherent interpretation of Kuhn＇s incommensurability-thesis. To that end, I distinguish in line with much of Kuhn-scholarship a semantic from a methodological sense of incommensurability. In part B, the question is raised： What sort of epistemological consequences follow from both senses of incommensurability？ In particular, what consequences follow for the issues of reference, subjectivity （objectivity）, pluralism, and realism？ The underlying question is to what extent Kuhnian incommensurability caters to a relativistic understanding of those issues. This question is answered in Paragraph Ill with the help of the analyses of a currently leading Kuhn-scholar, C. H. Sankey. His answers are taken as a vantage point for my concluding evaluation of the consequences of Kuhnian incommensurability in Paragraph IV.
