[Objective] The genetic diversity of major mango cultivars in China was analyzed by using SSR markers, and their fingerprints were constructed so as to provide theoretical basis for germplasm innovation and breeding o...[Objective] The genetic diversity of major mango cultivars in China was analyzed by using SSR markers, and their fingerprints were constructed so as to provide theoretical basis for germplasm innovation and breeding of mango. [Method] With 115 pairs of SSR primers, genetic diversity analysis and cluster analysis were performed for 30 mango cultivars, among which the genetic relationships were analyzed. [Result] Total 64 pairs of polymorphic primers were screened out from the 115 pairs of primers, and total 343 bands were amplified from the 30 cultivars with 73.2% of polymorphic bands. On average, 3.9 allelic loci were detected for each pair of primers with genetic diversity index of 0.5, Shannon＇s diversity index of 1.00 and polymorphism information content of 0.49, indicating higher genetic diversity. The cluster analysis showed that the 30 major cultivars could be classified into four categories. The first category included 14 cultivars; the second category included 11 cultivars, most of which were introduced from abroad; the third category included 4 cultivars, Le., Miansan, Parayinda, Baiyu and Hongxiangya： the fourth category included only one cultivar Maqiesu.By using 7 pairs of SSR markers, i.e., M42, M49, M54, M55, M96, M99 and M103, digital fingerprints were constructed for the 30 mango cultivars. [Conclusion] The 30 mango cultivars present more complex genomic genetics and abundant genetic information, and they have higher genetic diversity.展开更多
Objective: To investigate the predictability of MRI and the possiblebiological markers of cavernous sinus invasion of pituitary adenomas associated with fourphenomenas: angiogenesis, cell proliferation, apoptosis and ...Objective: To investigate the predictability of MRI and the possiblebiological markers of cavernous sinus invasion of pituitary adenomas associated with fourphenomenas: angiogenesis, cell proliferation, apoptosis and matrix metalloproteinase. Methods: Weevaluated 45 patients with pituitary adenoma according to the MRI, surgical findings and theimmunohistochemistry staining of tumor tissues. Results: The results have shown that the sensitivityof MRI for predicting cavernous sinus invasion in this prospective study was 60%, its specificity85%, its positive predictive value 83.33%, negative predictive value 62.96%. 45 specimens ofpituitary adenomas were analyzed for expression of F8, VEGF, Ki-67, c-myc, Bcl-2, nm23 and MMP-9immunoreactivity using immunoperoxidase staining. MVD was assessed using F8-related antigen. Theresults have shown that MVD of invasive pituitary adenomas was significantly higher than that ofnoninvasive (P 【 0.001). There was an association between the invasion of pituitary adenomas andKi-67 LI (P = 0.039) or the expression of VEGF (P 【 0.001) and MMP-9 (P 【 0.001). But c-myc LI andBcl-2 expression have no association with invasiveness of pituitary adenomas (P = 0.061 versus P =0.201). On the other hand, there is an inverse relationship between nm23 expression and tumorinvasion (P 【 0.001). Conclusion: Parasellar extension of pituitary adenomas through the medial wallof the cavernous sinus is diagnosed at surgery, and with sensitive gadolinium-enhanced MRI, itsextent can be partly determined by radiology. Although our study has shown that MVD and theexpression of VEGF, Ki-67, nm23 and MMP-9 have associations with invasiveness of pituitary adenomas,they are lack of specificity. These markers can only provide some useful information.展开更多
基金Supported by Natural Science Foundation of Hainan Province(34128)Fundamental Scientific Research Funds of Chinese Academy of Tropical Agricultural Sciences(1630032013031)~~
文摘[Objective] The genetic diversity of major mango cultivars in China was analyzed by using SSR markers, and their fingerprints were constructed so as to provide theoretical basis for germplasm innovation and breeding of mango. [Method] With 115 pairs of SSR primers, genetic diversity analysis and cluster analysis were performed for 30 mango cultivars, among which the genetic relationships were analyzed. [Result] Total 64 pairs of polymorphic primers were screened out from the 115 pairs of primers, and total 343 bands were amplified from the 30 cultivars with 73.2% of polymorphic bands. On average, 3.9 allelic loci were detected for each pair of primers with genetic diversity index of 0.5, Shannon＇s diversity index of 1.00 and polymorphism information content of 0.49, indicating higher genetic diversity. The cluster analysis showed that the 30 major cultivars could be classified into four categories. The first category included 14 cultivars; the second category included 11 cultivars, most of which were introduced from abroad; the third category included 4 cultivars, Le., Miansan, Parayinda, Baiyu and Hongxiangya： the fourth category included only one cultivar Maqiesu.By using 7 pairs of SSR markers, i.e., M42, M49, M54, M55, M96, M99 and M103, digital fingerprints were constructed for the 30 mango cultivars. [Conclusion] The 30 mango cultivars present more complex genomic genetics and abundant genetic information, and they have higher genetic diversity.
文摘Objective: To investigate the predictability of MRI and the possiblebiological markers of cavernous sinus invasion of pituitary adenomas associated with fourphenomenas: angiogenesis, cell proliferation, apoptosis and matrix metalloproteinase. Methods: Weevaluated 45 patients with pituitary adenoma according to the MRI, surgical findings and theimmunohistochemistry staining of tumor tissues. Results: The results have shown that the sensitivityof MRI for predicting cavernous sinus invasion in this prospective study was 60%, its specificity85%, its positive predictive value 83.33%, negative predictive value 62.96%. 45 specimens ofpituitary adenomas were analyzed for expression of F8, VEGF, Ki-67, c-myc, Bcl-2, nm23 and MMP-9immunoreactivity using immunoperoxidase staining. MVD was assessed using F8-related antigen. Theresults have shown that MVD of invasive pituitary adenomas was significantly higher than that ofnoninvasive (P 【 0.001). There was an association between the invasion of pituitary adenomas andKi-67 LI (P = 0.039) or the expression of VEGF (P 【 0.001) and MMP-9 (P 【 0.001). But c-myc LI andBcl-2 expression have no association with invasiveness of pituitary adenomas (P = 0.061 versus P =0.201). On the other hand, there is an inverse relationship between nm23 expression and tumorinvasion (P 【 0.001). Conclusion: Parasellar extension of pituitary adenomas through the medial wallof the cavernous sinus is diagnosed at surgery, and with sensitive gadolinium-enhanced MRI, itsextent can be partly determined by radiology. Although our study has shown that MVD and theexpression of VEGF, Ki-67, nm23 and MMP-9 have associations with invasiveness of pituitary adenomas,they are lack of specificity. These markers can only provide some useful information.
