[Objective] This study aimed to reconstruct the HP intein by overlap-exten- sion PCR. [Method] Several primers were designed according to the principle of overlap-extension PCR that the repeat sequences were overlappe...[Objective] This study aimed to reconstruct the HP intein by overlap-exten- sion PCR. [Method] Several primers were designed according to the principle of overlap-extension PCR that the repeat sequences were overlapped and served as the template to the other DNA strand in amplification. Then, several rounds of over- lap PCR reaction were conducted to mutate the four cysteines in the HP intein into serines, and introduce a linker sequence containing a tag for product detection and purification. [Result] DNA sequencing analysis proved that the four cysteines in the HP intein were successfully mutated into serines; the PCR precursor fragments were also rejoined into an intact HP gene fragment, without introducing any other unex- pected mutation; the DNA linker of 46 bp was successfully inserted into the de- signed HP gene sequences, without any mutation and base pair mismatch. [Conclu- sion] The HP intein was rapidly reconstructed by overlap-extension PCR in this study, which not only expands the application of overlap PCR in protein engineering, but also provides a simple, efficient and highly accurate tool for the reconstruction and modification of intein.展开更多
Froth flotation is used widely for upgrading raw phosphate.The flotation recovery of coarse phosphate(-1.18+0.425 mm) is much lower than that achieved on the-0.425+0.15 mm size fraction.Enhanced recovery of coarse pho...Froth flotation is used widely for upgrading raw phosphate.The flotation recovery of coarse phosphate(-1.18+0.425 mm) is much lower than that achieved on the-0.425+0.15 mm size fraction.Enhanced recovery of coarse phosphate particles is of great economic and environmental importance for phosphate industry.In this investigation,four different phosphate samples were aquired,characterized and tested in a specially designed laboratory-scale flotation column.Significant recovery improvement of coarse phosphate flotation was achieved using cavitation-generated nanobubble though its effects differ among the four testing phosphate samples.The laboratory-scale flotation column test results indicate that nanobubble increased P_2O_5 recovery by up to 10%~30%for a given Acid Insoluble(A.I.) rejection,depending on the characteristic of phosphate samples.The improvement effect of nanobubble on the hard-to-float particles was more significant than that on easy-to-float particles,especially at lower collector dosages.Nanobubbles reduced the collector dosage by 1/3 to 1/2.Nanobubbles almost doubled the coarse phosphate flotation rate constant and increased the flotation selectivity index by up to 25%.展开更多
Liquefied natural gas(LNG) is the most economical way of transporting natural gas(NG) over long distances. Liquefaction of NG using vapor compression refrigeration system requires high operating and capital cost. Due ...Liquefied natural gas(LNG) is the most economical way of transporting natural gas(NG) over long distances. Liquefaction of NG using vapor compression refrigeration system requires high operating and capital cost. Due to lack of systematic design methods for multistage refrigeration cycles, conventional approaches to determine optimal cycle are largely trial-and-error. In this paper a novel mixed integer non-linear programming(MINLP)model is introduced to select optimal synthesis of refrigeration systems to reduce both operating and capital costs of an LNG plant. Better conceptual understanding of design improvement is illustrated on composite curve(CC) and exergetic grand composite curve(EGCC) of pinch analysis diagrams. In this method a superstructure representation of complex refrigeration system is developed to select and optimize key decision variables in refrigeration cycles(i.e. partition temperature, compression configuration, refrigeration features, refrigerant flow rate and economic trade-off). Based on this method a program(LNG-Pro) is developed which integrates VBA,Refprop and Excel MINLP Solver to automate the methodology. Design procedure is applied on a sample LNG plant to illustrate advantages of using this method which shows a 3.3% reduction in total shaft work consumption.展开更多
To determine how bus stop design influences mixed traffic operation near Chinese bus stops,a new theoretical method was developed by using additive-conflict-flows procedure.The procedure was extended from homogeneous ...To determine how bus stop design influences mixed traffic operation near Chinese bus stops,a new theoretical method was developed by using additive-conflict-flows procedure.The procedure was extended from homogeneous traffic flow to mixed traffic flow.Based on the procedure and queuing theory,car capacity and speed models were proposed for three types of bus stops including curbside,bus bay and bicycle detour.The effects of various combinations of bus stop type,traffic volume,bus dwell time,and berth number on traffic operations were investigated.The results indicate that traffic volume,bus dwell time and berth number have negative effects on traffic operations for any type of bus stops.For different types of bus stops,at car volumes above approximately 200 vehicles per hour,the bus bay and bicycle detour designs provide more benefits than the curbside design.As traffic volume increases,the benefit firstly increases in uncongested conditions and then decreases in congested conditions.It reaches the maximum at car volumes nearly 1 100 vehicles per hour.The results can be used to aid in the selection of a preferred bus stop design for a given traffic volume in developing countries.展开更多
Based on the information system characteristics of mine, proposed network architecture design of the mine property. And in this framework based on the design of three-dimensional virtual mine described the application...Based on the information system characteristics of mine, proposed network architecture design of the mine property. And in this framework based on the design of three-dimensional virtual mine described the application of intelligent management platform. Three-dimensional virtual underground mine that shows the situation, the core application is through remote monitoring system of information exchange between devices (material object communication). Internet of things in the framework of mining three-dimensional virtual reconstruction of mine. On coal mine safety in the production process of human, machine and environment, control elements and their harmony and unity. 3D virtual mine management platform integrates personnel positioning, dust control, gas monitoring, roof pressure monitoring, fan-line monitoring and other subsystems. Platform through the underground mine sensing equipment to conduct various types of monitoring data integration, through the transport layer device to transmit the information to the application layer intelligence processing software platform, the system automatically handles the operational status of each subsystem and the need for safe production under the proper introduction of human factors deal with special event. 3D virtual mine management platform to mining, excavation, transport, ventilation and other safety information quickly and accurately transmitted to the ground operation control center. Underground for the first time on the linkage between systems in case of emergencies, to provide safety for management decision support.展开更多
Glycerol is one of the most important biomass-based platform molecules,massively produced as a by-product in the biodiesel industry.Its high purification cost from the crude glycerol raw material limits its applicatio...Glycerol is one of the most important biomass-based platform molecules,massively produced as a by-product in the biodiesel industry.Its high purification cost from the crude glycerol raw material limits its application and demands new strategies for valorization.Compared to the conventional thermocatalytic strategies,the electrocatalytic strategies can not only enable the selective conversion at mild conditions but also pair up the cathodic reactions for the co-production with higher efficiencies.In this review,we summarize the recent advances of catalyst designs and mechanistic understandings for the electrocatalytic glycerol oxidation(GOR),and aim to provide an overview of the GOR process and the intrinsic structural-activity correlation for inspiring future work in this field.The review is dissected into three sections.We will first introduce the recent efforts of designing more efficient and selective catalysts for GOR,especially toward the production of value-added products.Then,we will summarize the current understandings about the reaction network based on the ex-situ and in-situ spectroscopic studies as well as the theoretical works.Lastly,we will select some representative examples of creating real electrochemical devices for the valorization of glycerol.By summarizing these previous efforts,we will provide our vision of future directions in the field of GOR toward real applications.展开更多
The Saharan areas and in particular the isolated sites are characterized by low levels of population, in dispersed cores, which suffer from serious problems of water supply for consumption and the irrigation. The phot...The Saharan areas and in particular the isolated sites are characterized by low levels of population, in dispersed cores, which suffer from serious problems of water supply for consumption and the irrigation. The photovoltaic pumping systems (PV) are an ideal solution for these problems. Despite the presence of several photovoltaic pumping systems (PVPS) projects, data acquisition system is still missing in most cases. In this work, the authors will present the PVPS installed at the unit of research in renewable energies in Saharan medium (URERMS, Algeria). This system will enable us to better analyze the performances ofa photovoltaic pumping system in Saharan medium. The principal elements of the chain of the photovoltaic pumping system are: a photovoltaic generator, the power conditioning system, a motor-driven pump immersed group, an electric wiring, a hydraulic infrastructure which comprises a water tank.展开更多
This paper focuses on the design and implementation of an active multibeam antenna system for massive MIMO applications in 5G wireless communications.The highly integrated active multibeam antenna system is designed a...This paper focuses on the design and implementation of an active multibeam antenna system for massive MIMO applications in 5G wireless communications.The highly integrated active multibeam antenna system is designed and implemented at 5.8 GHz with 64 RF Channels and 256 antenna elements.The 64-channel highly integrated active multibeam antenna system provides a verification platform for digital beamforming algorithm and massive MIMO channel estimation for next generation wireless communications.展开更多
This paper reviews recent advances in radar sensor design for low-power healthcare,indoor real-time positioning and other applications of IoT.Various radar front-end architectures and digital processing methods are pr...This paper reviews recent advances in radar sensor design for low-power healthcare,indoor real-time positioning and other applications of IoT.Various radar front-end architectures and digital processing methods are proposed to improve the detection performance including detection accuracy,detection range and power consumption.While many of the reported designs were prototypes for concept verification,several integrated radar systems have been demonstrated with reliable measured results with demo systems.A performance comparison of latest radar chip designs has been provided to show their features of different architectures.With great development of IoT,short-range low-power radar sensors for healthcare and indoor positioning applications will attract more and more research interests in the near future.展开更多
The architecture of footbridge design takes the form of a number of submissions from leading architects and engineers, each setting out their views on bridge design--present and future. It looks at the functions of a ...The architecture of footbridge design takes the form of a number of submissions from leading architects and engineers, each setting out their views on bridge design--present and future. It looks at the functions of a bridge, defining purpose of place and context, the spirit of creativity and the reasoned progression of an idea. It also explores the exploitation of materials technology and construction innovation and the tension between lightness and mass and between sculpture and scale. Present parameters of tempered and laminated glass create possibility of modern architecture of footbridges which are being full of transparency and light reflex effects. Four projects, using glass panels designed by Santiago Calatrava, have been presented in this paper. GFRP (glass fiber retrofit polymer) makes new horizon in material technology, helps to enrich new conception of structure with longer durability, low weight of deck and new creation of architecture line. The paper has described a few results of FEM (finite element method) analysis of footbridge with modular bridge GFRP deck system. The footbridge was excited by impact and human-induced vibrations. Composite material consists of glass fibers and polymer matrix is a promising alternative against traditional materials. New architecture and modern material engineering are looking for fresh possibilities of form and shape of structure, long durability and extraordinary technical parameters of building elements.展开更多
To quantitatively identify the maintenance demand for each highway segments in the pavement maintenance scheme design,a mathematical model of uniform segment division was established and an approach of applying cluste...To quantitatively identify the maintenance demand for each highway segments in the pavement maintenance scheme design,a mathematical model of uniform segment division was established and an approach of applying cluster analysis theory to the uniform segment division and evaluation of pavement maintenance demand was proposed.The actual maintenance project of a highway carried out in Guangdong province was cited as an example to demonstrate the validity of the proposed method.It is proved that the cluster analysis can eliminate human factors in classification without being constrained by the quantities of samples,considering multiple pavement distress indexes and the continuity of samples.Thus it is evident that cluster analysis is an efficient analytical tool in uniform segment division and evaluation of maintenance demand.展开更多
Similarity measure design for discrete data group was proposed. Similarity measure design for continuous membership function was also carried out. Proposed similarity measures were designed based on fuzzy number and d...Similarity measure design for discrete data group was proposed. Similarity measure design for continuous membership function was also carried out. Proposed similarity measures were designed based on fuzzy number and distance measure, and were proved. To calculate the degree of similarity of discrete data, relative degree between data and total distribution was obtained. Discrete data similarity measure was completed with combination of mentioned relative degrees. Power interconnected system with multi characteristics was considered to apply discrete similarity measure. Naturally, similarity measure was extended to multi-dimensional similarity measure case, and applied to bus clustering problem.展开更多
基金Supported by the National High Technology Research and Development Program of China(863Program)(2006AA03Z451)Shanghai Basic Research Key Program(10JC1400300)+1 种基金National Natural Science Foundation of China(31070698)the Fundamental Research Funds for the Central Universities(12D10520)~~
文摘[Objective] This study aimed to reconstruct the HP intein by overlap-exten- sion PCR. [Method] Several primers were designed according to the principle of overlap-extension PCR that the repeat sequences were overlapped and served as the template to the other DNA strand in amplification. Then, several rounds of over- lap PCR reaction were conducted to mutate the four cysteines in the HP intein into serines, and introduce a linker sequence containing a tag for product detection and purification. [Result] DNA sequencing analysis proved that the four cysteines in the HP intein were successfully mutated into serines; the PCR precursor fragments were also rejoined into an intact HP gene fragment, without introducing any other unex- pected mutation; the DNA linker of 46 bp was successfully inserted into the de- signed HP gene sequences, without any mutation and base pair mismatch. [Conclu- sion] The HP intein was rapidly reconstructed by overlap-extension PCR in this study, which not only expands the application of overlap PCR in protein engineering, but also provides a simple, efficient and highly accurate tool for the reconstruction and modification of intein.
基金the Florida Institute of Phosphate Research(FIPR),The Center for Advanced Separation Technologies(CAST) and the National Natural Science Foundation of China (Nos.50921002 and 90510002) for the financial supportCF Industry Inc and Mosaic Company for supplying specimens and ArrMaz Custom Chemicals Inc.for providing chemicals employed in this study.
文摘Froth flotation is used widely for upgrading raw phosphate.The flotation recovery of coarse phosphate(-1.18+0.425 mm) is much lower than that achieved on the-0.425+0.15 mm size fraction.Enhanced recovery of coarse phosphate particles is of great economic and environmental importance for phosphate industry.In this investigation,four different phosphate samples were aquired,characterized and tested in a specially designed laboratory-scale flotation column.Significant recovery improvement of coarse phosphate flotation was achieved using cavitation-generated nanobubble though its effects differ among the four testing phosphate samples.The laboratory-scale flotation column test results indicate that nanobubble increased P_2O_5 recovery by up to 10%~30%for a given Acid Insoluble(A.I.) rejection,depending on the characteristic of phosphate samples.The improvement effect of nanobubble on the hard-to-float particles was more significant than that on easy-to-float particles,especially at lower collector dosages.Nanobubbles reduced the collector dosage by 1/3 to 1/2.Nanobubbles almost doubled the coarse phosphate flotation rate constant and increased the flotation selectivity index by up to 25%.
文摘Liquefied natural gas(LNG) is the most economical way of transporting natural gas(NG) over long distances. Liquefaction of NG using vapor compression refrigeration system requires high operating and capital cost. Due to lack of systematic design methods for multistage refrigeration cycles, conventional approaches to determine optimal cycle are largely trial-and-error. In this paper a novel mixed integer non-linear programming(MINLP)model is introduced to select optimal synthesis of refrigeration systems to reduce both operating and capital costs of an LNG plant. Better conceptual understanding of design improvement is illustrated on composite curve(CC) and exergetic grand composite curve(EGCC) of pinch analysis diagrams. In this method a superstructure representation of complex refrigeration system is developed to select and optimize key decision variables in refrigeration cycles(i.e. partition temperature, compression configuration, refrigeration features, refrigerant flow rate and economic trade-off). Based on this method a program(LNG-Pro) is developed which integrates VBA,Refprop and Excel MINLP Solver to automate the methodology. Design procedure is applied on a sample LNG plant to illustrate advantages of using this method which shows a 3.3% reduction in total shaft work consumption.
基金Project(2012CB725400) supported by the National Basic Research Program of ChinaProjects(70901005, 71071016, 71131001) supported by the National Natural Science Foundation of ChinaProject(2011JBM055) supported by the Fundamental Research Funds for the Central Universities of China
文摘To determine how bus stop design influences mixed traffic operation near Chinese bus stops,a new theoretical method was developed by using additive-conflict-flows procedure.The procedure was extended from homogeneous traffic flow to mixed traffic flow.Based on the procedure and queuing theory,car capacity and speed models were proposed for three types of bus stops including curbside,bus bay and bicycle detour.The effects of various combinations of bus stop type,traffic volume,bus dwell time,and berth number on traffic operations were investigated.The results indicate that traffic volume,bus dwell time and berth number have negative effects on traffic operations for any type of bus stops.For different types of bus stops,at car volumes above approximately 200 vehicles per hour,the bus bay and bicycle detour designs provide more benefits than the curbside design.As traffic volume increases,the benefit firstly increases in uncongested conditions and then decreases in congested conditions.It reaches the maximum at car volumes nearly 1 100 vehicles per hour.The results can be used to aid in the selection of a preferred bus stop design for a given traffic volume in developing countries.
文摘Based on the information system characteristics of mine, proposed network architecture design of the mine property. And in this framework based on the design of three-dimensional virtual mine described the application of intelligent management platform. Three-dimensional virtual underground mine that shows the situation, the core application is through remote monitoring system of information exchange between devices (material object communication). Internet of things in the framework of mining three-dimensional virtual reconstruction of mine. On coal mine safety in the production process of human, machine and environment, control elements and their harmony and unity. 3D virtual mine management platform integrates personnel positioning, dust control, gas monitoring, roof pressure monitoring, fan-line monitoring and other subsystems. Platform through the underground mine sensing equipment to conduct various types of monitoring data integration, through the transport layer device to transmit the information to the application layer intelligence processing software platform, the system automatically handles the operational status of each subsystem and the need for safe production under the proper introduction of human factors deal with special event. 3D virtual mine management platform to mining, excavation, transport, ventilation and other safety information quickly and accurately transmitted to the ground operation control center. Underground for the first time on the linkage between systems in case of emergencies, to provide safety for management decision support.
文摘Glycerol is one of the most important biomass-based platform molecules,massively produced as a by-product in the biodiesel industry.Its high purification cost from the crude glycerol raw material limits its application and demands new strategies for valorization.Compared to the conventional thermocatalytic strategies,the electrocatalytic strategies can not only enable the selective conversion at mild conditions but also pair up the cathodic reactions for the co-production with higher efficiencies.In this review,we summarize the recent advances of catalyst designs and mechanistic understandings for the electrocatalytic glycerol oxidation(GOR),and aim to provide an overview of the GOR process and the intrinsic structural-activity correlation for inspiring future work in this field.The review is dissected into three sections.We will first introduce the recent efforts of designing more efficient and selective catalysts for GOR,especially toward the production of value-added products.Then,we will summarize the current understandings about the reaction network based on the ex-situ and in-situ spectroscopic studies as well as the theoretical works.Lastly,we will select some representative examples of creating real electrochemical devices for the valorization of glycerol.By summarizing these previous efforts,we will provide our vision of future directions in the field of GOR toward real applications.
文摘The Saharan areas and in particular the isolated sites are characterized by low levels of population, in dispersed cores, which suffer from serious problems of water supply for consumption and the irrigation. The photovoltaic pumping systems (PV) are an ideal solution for these problems. Despite the presence of several photovoltaic pumping systems (PVPS) projects, data acquisition system is still missing in most cases. In this work, the authors will present the PVPS installed at the unit of research in renewable energies in Saharan medium (URERMS, Algeria). This system will enable us to better analyze the performances ofa photovoltaic pumping system in Saharan medium. The principal elements of the chain of the photovoltaic pumping system are: a photovoltaic generator, the power conditioning system, a motor-driven pump immersed group, an electric wiring, a hydraulic infrastructure which comprises a water tank.
文摘This paper focuses on the design and implementation of an active multibeam antenna system for massive MIMO applications in 5G wireless communications.The highly integrated active multibeam antenna system is designed and implemented at 5.8 GHz with 64 RF Channels and 256 antenna elements.The 64-channel highly integrated active multibeam antenna system provides a verification platform for digital beamforming algorithm and massive MIMO channel estimation for next generation wireless communications.
文摘This paper reviews recent advances in radar sensor design for low-power healthcare,indoor real-time positioning and other applications of IoT.Various radar front-end architectures and digital processing methods are proposed to improve the detection performance including detection accuracy,detection range and power consumption.While many of the reported designs were prototypes for concept verification,several integrated radar systems have been demonstrated with reliable measured results with demo systems.A performance comparison of latest radar chip designs has been provided to show their features of different architectures.With great development of IoT,short-range low-power radar sensors for healthcare and indoor positioning applications will attract more and more research interests in the near future.
文摘The architecture of footbridge design takes the form of a number of submissions from leading architects and engineers, each setting out their views on bridge design--present and future. It looks at the functions of a bridge, defining purpose of place and context, the spirit of creativity and the reasoned progression of an idea. It also explores the exploitation of materials technology and construction innovation and the tension between lightness and mass and between sculpture and scale. Present parameters of tempered and laminated glass create possibility of modern architecture of footbridges which are being full of transparency and light reflex effects. Four projects, using glass panels designed by Santiago Calatrava, have been presented in this paper. GFRP (glass fiber retrofit polymer) makes new horizon in material technology, helps to enrich new conception of structure with longer durability, low weight of deck and new creation of architecture line. The paper has described a few results of FEM (finite element method) analysis of footbridge with modular bridge GFRP deck system. The footbridge was excited by impact and human-induced vibrations. Composite material consists of glass fibers and polymer matrix is a promising alternative against traditional materials. New architecture and modern material engineering are looking for fresh possibilities of form and shape of structure, long durability and extraordinary technical parameters of building elements.
基金Sponsored by the Scientific and Technological Project on Road Maintenance Management Mode in Guangdong Province(Grant No.200407132)the Launching Fund Project for Dr.in Guangdong Province(Grant No.05300135)
文摘To quantitatively identify the maintenance demand for each highway segments in the pavement maintenance scheme design,a mathematical model of uniform segment division was established and an approach of applying cluster analysis theory to the uniform segment division and evaluation of pavement maintenance demand was proposed.The actual maintenance project of a highway carried out in Guangdong province was cited as an example to demonstrate the validity of the proposed method.It is proved that the cluster analysis can eliminate human factors in classification without being constrained by the quantities of samples,considering multiple pavement distress indexes and the continuity of samples.Thus it is evident that cluster analysis is an efficient analytical tool in uniform segment division and evaluation of maintenance demand.
基金Project(2010-0020163) supported by Key Research Institute Program through the National Research Foundation of Korea (NRF) funded by the Ministry of Education, Science and Technology, Korea
文摘Similarity measure design for discrete data group was proposed. Similarity measure design for continuous membership function was also carried out. Proposed similarity measures were designed based on fuzzy number and distance measure, and were proved. To calculate the degree of similarity of discrete data, relative degree between data and total distribution was obtained. Discrete data similarity measure was completed with combination of mentioned relative degrees. Power interconnected system with multi characteristics was considered to apply discrete similarity measure. Naturally, similarity measure was extended to multi-dimensional similarity measure case, and applied to bus clustering problem.