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RoboCup中NAO机器人球场目标红球识别与快速找球策略 被引量:6
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作者 孟宪龙 李龙澍 罗罹 《电脑知识与技术》 2013年第8X期5515-5519,共5页
根据RoboCup中NAO机器人足球比赛的规则,机器人通过对颜色的识别来识别球场上的物体及环境。文章分析了RGB颜色空间的优缺点,采用HSV作为视觉系统的颜色空间,减弱了现场光照变化对视觉系统产生的影响。文章提出了一种基于HSV色彩空间下... 根据RoboCup中NAO机器人足球比赛的规则,机器人通过对颜色的识别来识别球场上的物体及环境。文章分析了RGB颜色空间的优缺点,采用HSV作为视觉系统的颜色空间,减弱了现场光照变化对视觉系统产生的影响。文章提出了一种基于HSV色彩空间下识别红球的方法,然后提出一种简单的追踪红球策略,与测距追踪实际效果相比较,发现这种追踪策略更符合实际场景应用。 展开更多
关键词 NAO机器人 目标红识别 目标追踪 图像处理
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基于球心点互斥的球目标识别方法 被引量:1
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作者 李子宽 廖威 蓝秋萍 《图学学报》 CSCD 北大核心 2018年第1期50-56,共7页
提出了一种基于球心点互斥的球目标识别方法,用于从大场景三维点云中自动识别未知个数和未知半径的球目标。首先,根据专门设计的球面点响应函数滤除大量非球面点,并根据法向与曲率将剩余的球面点映射到球心位置;然后,构建用以描述局部... 提出了一种基于球心点互斥的球目标识别方法,用于从大场景三维点云中自动识别未知个数和未知半径的球目标。首先,根据专门设计的球面点响应函数滤除大量非球面点,并根据法向与曲率将剩余的球面点映射到球心位置;然后,构建用以描述局部密度渐变规律的球心点互斥树,通过剪枝操作将其分裂成若干子树,其分别对应不同球目标的球心点聚类;最后,根据球心点局部密度和球面点覆盖率估计值确认真实存在的球目标。实验结果表明:基于球心点互斥的球目标识别方法能够有效解决大场景三维点云中球目标的识别问题,即使是存在严重遮挡的情况下,暴露表面不足整个球面6%的球目标也都能够被识别出来。 展开更多
关键词 目标识别 面点响应函数 心点互斥 聚类 面覆盖率
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基于超球覆盖仿生模式识别的指纹点名系统 被引量:1
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作者 李丽 殷业 崔晓静 《上海师范大学学报(自然科学版)》 2015年第1期81-87,共7页
超球覆盖仿生模式识别方法具有识别速度快、误识率低、易于实现等特点,特别适合于一些实用性较强的应用领域使用.研究了一种基于超球串覆盖的仿生模式识别方法,并应用于指纹点名系统.在对实际教学需求分析的基础上,设计了完整的指纹点... 超球覆盖仿生模式识别方法具有识别速度快、误识率低、易于实现等特点,特别适合于一些实用性较强的应用领域使用.研究了一种基于超球串覆盖的仿生模式识别方法,并应用于指纹点名系统.在对实际教学需求分析的基础上,设计了完整的指纹点名系统软硬件,通过指纹传感器对指纹数据进行采集、预处理、用超球串覆盖算法对指纹图像进行了识别,在嵌入式系统STM32中实现了指纹注册、班级选择、指纹签到、签到次数记录等功能,实验结果显示系统达到预期的效果,解决了课堂实时点名问题.超球串覆盖仿生模式识别方法适用于众多应用领域,通过示例,可以举一反三,将方法复制到其他应用场合. 展开更多
关键词 覆盖仿生模式识别 指纹识别 嵌入式系统 指纹点名系统
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基于STM32图像识别的板球系统的设计 被引量:3
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作者 段凌飞 姚敏 张宏桥 《机电工程技术》 2019年第8期162-164,280,共4页
针对板球系统结构复杂、设备昂贵的问题,设计了基于STM32F407处理器和数字CMOS摄像头的简易可靠的嵌入式板球系统。阐述了系统机械结构组成及控制系统原理详细说明,运用了嵌入式的高效小球识别图形处理算法。通过对系统特性的分析,采用... 针对板球系统结构复杂、设备昂贵的问题,设计了基于STM32F407处理器和数字CMOS摄像头的简易可靠的嵌入式板球系统。阐述了系统机械结构组成及控制系统原理详细说明,运用了嵌入式的高效小球识别图形处理算法。通过对系统特性的分析,采用模糊抗积分饱和PID控制算法进行控制。通过制作后测试,设计的机械结构稳定可靠,控制算法具有良好的鲁棒性。 展开更多
关键词 系统 识别算法 拉普拉斯算子 抗积分饱和模糊PID
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基于模糊免疫识别方法的电力变压器故障诊断 被引量:15
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作者 周爱华 张彼德 +1 位作者 方春恩 李伟 《电网技术》 EI CSCD 北大核心 2009年第3期99-102,共4页
在人工免疫识别系统的基础上,结合模糊推理,提出了基于模糊免疫识别的充油变压器故障诊断方法。代表相同B细胞的人工识别球经克隆选择和资源竞争后产生具有分类性能的记忆抗体,但其资源分配数目和人工识别球的刺激水平成线性,易使少数... 在人工免疫识别系统的基础上,结合模糊推理,提出了基于模糊免疫识别的充油变压器故障诊断方法。代表相同B细胞的人工识别球经克隆选择和资源竞争后产生具有分类性能的记忆抗体,但其资源分配数目和人工识别球的刺激水平成线性,易使少数优秀人工识别球占据过多资源,引起人工识别球种群早熟收敛。将模糊推理应用于资源分配机制中,可使分配兼顾刺激水平和抑制水平,改善收敛性能。实例分析表明,以H2、CH4、C2H4、C2H6、C2H2等5种气体含量为特征参数,将模糊推理与人工免疫识别系统相结合的方法,能有效诊断变压器内部潜伏性故障。 展开更多
关键词 电力变压器 故障诊断 免疫识别 模糊推理 识别球(ARB)
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拖拉机驾驶室内噪声源识别测试与分析 被引量:2
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作者 郑志昊 齐兵兵 +1 位作者 陈俊杰 王志鹏 《噪声与振动控制》 CSCD 2018年第6期72-75,共4页
为研究驾驶室内噪声问题,采用球型阵列进行拖拉机驾驶室内噪声源定位测试,分析结果表明驾驶室内声源位置主要在门锁机构附近和操纵机构附近,且在车门玻璃处出现较大的反射噪声。运用声强探头对声源泄漏位置进行特定的声强测试,以此来分... 为研究驾驶室内噪声问题,采用球型阵列进行拖拉机驾驶室内噪声源定位测试,分析结果表明驾驶室内声源位置主要在门锁机构附近和操纵机构附近,且在车门玻璃处出现较大的反射噪声。运用声强探头对声源泄漏位置进行特定的声强测试,以此来分析驾驶室内噪声源产生的具体原因,为下一步改善拖拉机驾驶室内的声学环境打下基础。 展开更多
关键词 声学 拖拉机驾驶室 声源识别 阵列 声强测试
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类人足球机器人目标球检测方法研究
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作者 徐晓凤 王君 +1 位作者 董莹 谢瑞亲 《机器人技术与应用》 2017年第2期37-40,共4页
针对RoboCup比赛规则的变化,本文提出了一种颜色特征约束与几何约束相结合的目标球检测方法。该方法结合类人足球机器人平台上的视觉传感器在有效提高识别精度的同时,扩大了有效识别区域的面积,可以满足机器人比赛中实时追踪足球目标的... 针对RoboCup比赛规则的变化,本文提出了一种颜色特征约束与几何约束相结合的目标球检测方法。该方法结合类人足球机器人平台上的视觉传感器在有效提高识别精度的同时,扩大了有效识别区域的面积,可以满足机器人比赛中实时追踪足球目标的需求。 展开更多
关键词 机器人 ROBOCUP 目标识别 机器视觉
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基于人工免疫分类算法的电力变压器故障诊断 被引量:15
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作者 周爱华 张彼德 张厚宣 《高电压技术》 EI CAS CSCD 北大核心 2007年第8期77-80,共4页
基于人工免疫识别原理提出一种电力变压器故障诊断方法。该算法模拟自然免疫中抗原和B细胞相互作用机制,将故障样本(变压器油中溶解气体体积)作为抗原,用加权欧氏距离计算亲和力,兼顾分量单项超标故障信息,通过免疫训练,获取表征故障样... 基于人工免疫识别原理提出一种电力变压器故障诊断方法。该算法模拟自然免疫中抗原和B细胞相互作用机制,将故障样本(变压器油中溶解气体体积)作为抗原,用加权欧氏距离计算亲和力,兼顾分量单项超标故障信息,通过免疫训练,获取表征故障样本的人工识别球集合,再用最邻近分类法对故障样本分类。实例表明,该算法能有效识别变压器故障,具有较高的检测准确率,在电力变压器故障诊断中有良好的应用前景。 展开更多
关键词 人工免疫 人工识别球 加权欧氏距离 变压器 故障诊断 最邻近分类法
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基于免疫Agent的液体火箭发动机故障诊断 被引量:3
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作者 张炜 明安波 +2 位作者 杨正伟 宋远佳 张瑞民 《计算机应用》 CSCD 北大核心 2009年第B12期272-274,278,共4页
为解决液体火箭发动机系统非线性导致的故障诊断困难问题,将免疫Agent(IA)技术应用于液体火箭发动机的故障诊断研究。结合可变半径人工识别球(ARB)方法,在学习人类举一反三的机制,采用最大识别概率原则的基础上,构建了免疫Agent实体,并... 为解决液体火箭发动机系统非线性导致的故障诊断困难问题,将免疫Agent(IA)技术应用于液体火箭发动机的故障诊断研究。结合可变半径人工识别球(ARB)方法,在学习人类举一反三的机制,采用最大识别概率原则的基础上,构建了免疫Agent实体,并进行了仿真实验。实验结果表明:该方法训练时间短、正确率高、有较强的抗噪能力和自学习能力,可以应用于液体火箭发动机的状态监测与故障诊断。 展开更多
关键词 免疫AGENT 人工识别球 液体火箭发动机 故障诊断
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基于人工免疫系统的关联规则挖掘算法 被引量:4
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作者 梁美莲 梁家荣 郭晨 《计算机应用》 CSCD 北大核心 2004年第8期50-53,共4页
给出了一个基于人工免疫系统的关联规则挖掘算法。将训练数据作为抗原 ,候选模式作为人工识别球 (ARB) ,通过免疫学习生成频繁模式并以免疫记忆的形式加以保存 ,最终生成关联规则。所给的应用实例说明本算法是可行。
关键词 人工免疫系统 AIS 人工识别球 数据挖掘 关联规则 频繁集
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优化记忆树的研究与实现
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作者 段友祥 王东亮 王志刚 《哈尔滨工程大学学报》 EI CAS CSCD 北大核心 2006年第B07期312-318,共7页
分析当前人工免疫算法和模型,特别是记忆检测细胞的匹配效率及随着时间推移细胞越来越多占用资源空间的问题.RLAIS模型没有将时间作为控制因子抑制资源的膨胀,不能很好解决随时间推移细胞占用资源越来越多的问题,其一些改进模型在... 分析当前人工免疫算法和模型,特别是记忆检测细胞的匹配效率及随着时间推移细胞越来越多占用资源空间的问题.RLAIS模型没有将时间作为控制因子抑制资源的膨胀,不能很好解决随时间推移细胞占用资源越来越多的问题,其一些改进模型在应用中可调节性也不高.为解决此问题而提出一优化记忆树模型.该模型特点:增加时间控制因子;对经常被匹配到的细胞的动态调整.利用时间控制条件和动态调整方法的记忆树模型既优化了记忆细胞的匹配效率,又优化了细胞资源空间,最终实现优化资源空间和提高效率的目的.最后实验验证本模型的可行性. 展开更多
关键词 人工免疫 识别球 记忆检测器 记忆树 效率优化
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AIS在LRE故障检测与诊断中的应用研究
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作者 张炜 明安波 +1 位作者 宋远佳 张瑞民 《上海航天》 2012年第2期42-47,54,共7页
为提高液体火箭发动机(LRE)故障检测与诊断的及时性、实时性与准确性,将人工免疫系统(AIS)中的否定选择原理用于LRE的故障检测与诊断。基于正常状态与故障模式的人工识别球,采用最大相似原则,实现了LRE稳态工作过程中的故障检测与诊断,... 为提高液体火箭发动机(LRE)故障检测与诊断的及时性、实时性与准确性,将人工免疫系统(AIS)中的否定选择原理用于LRE的故障检测与诊断。基于正常状态与故障模式的人工识别球,采用最大相似原则,实现了LRE稳态工作过程中的故障检测与诊断,并用LRE稳态故障试车数据进行实验。验证结果表明:AIS对LRE故障检测与诊断的速度快,正确率与灵敏度高,捕获未知故障能力强,有良好的应用前景。 展开更多
关键词 液体火箭发动机 人工免疫系统 故障检测与诊断 否定选择算法 人工识别球
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基于免疫抗体记忆分类算法的变压器故障诊断
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作者 张特 黄辉先 +1 位作者 张江锋 肖顿 《计算机工程》 CAS CSCD 北大核心 2011年第20期200-202,205,共4页
针对传统克隆免疫算法应用于变压器故障诊断时学习速度慢、无分类能力及抗体空间小等缺点,提出一种免疫抗体记忆分类算法。利用免疫抗体识别抗原的原理和抗体记忆功能,将克隆选择与进化算法相结合,通过学习训练抗原及附属类型信息,得到... 针对传统克隆免疫算法应用于变压器故障诊断时学习速度慢、无分类能力及抗体空间小等缺点,提出一种免疫抗体记忆分类算法。利用免疫抗体识别抗原的原理和抗体记忆功能,将克隆选择与进化算法相结合,通过学习训练抗原及附属类型信息,得到由不同类型检测集组成的故障信息库,从而在保证亲和力的同时,加快学习速度,扩大免疫搜索空间,并利用人工识别球与刺激水平的线性关系对故障类型进行快速诊断。实验结果证明,该算法具有较快的运行速度及较高的诊断准确率。 展开更多
关键词 克隆免疫 故障信息库 人工识别球 免疫抗体记忆分类 进化学习
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Temporal changes in physiological and performance responses across game-specific simulated basketball activity 被引量:3
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作者 Aaron T.Scanlan Jordan L.Fox +2 位作者 Nattai R.Borges Patrick S.Tucker Vincent J.Dalbo 《Journal of Sport and Health Science》 SCIE 2018年第2期176-182,共7页
Purpose: The aims of this study were to:(1) provide a comprehensive physiological profile of simulated basketball activity and(2) identify temporal changes in player responses in controlled settings.Methods: State-lev... Purpose: The aims of this study were to:(1) provide a comprehensive physiological profile of simulated basketball activity and(2) identify temporal changes in player responses in controlled settings.Methods: State-level male basketball players(n = 10) completed 4 × 10 min simulated quarters of basketball activity using a reliable and valid courtbased test. A range of physiological(ratings of perceived exertion, blood lactate concentration([BLa~—]), blood glucose concentration([BGlu]), heart rate(HR), and hydration) and physical(performance and fatigue indicators for sprint, circuit, and jump activity) measures were collected across testing.Results: Significantly reduced [BLa~—](6.19 ± 2.30 vs. 4.57 ± 2.33 mmol/L; p = 0.016) and [BGlu](6.91 ± 1.57 vs. 5.25 ± 0.81 mmol/L;p = 0.009) were evident in the second half. A mean HR of 180.1 ± 5.7 beats/min(90.8% ± 4.0% HR max) was observed, with a significant increase in vigorous activity(77%—95% HR max)(11.31 ± 6.91 vs. 13.50 ± 6.75 min; p = 0.024) and moderate decrease in near-maximal activity(>95% HR_(max))(7.24 ± 7.45 vs. 5.01 ± 7.20 min) in the second half. Small increases in performance times accompanied by a significantly lower circuit decrement(11.67% ± 5.55% vs. 7.30% ± 2.16%; p = 0.032) were apparent in the second half.Conclusion: These data indicate basketball activity imposes higher physiological demands than previously thought and temporal changes in responses might be due to adapted pacing strategies as well as fatigue-mediated mechanisms. 展开更多
关键词 CARDIOVASCULAR Game-play HYDRATION Rating of perceived exertion Simulation Team sports
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A NEW HYPERSPHERE SUPPORT VECTOR MACHINE ALGORITHM 被引量:2
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作者 Zhang Xinfeng Shen Lansun 《Journal of Electronics(China)》 2006年第4期614-617,共4页
The hypersphere support vector machine is a new algorithm in pattern recognition. By studying three kinds of hypersphere support vector machines, it is found that their solutions are identical and the margin between t... The hypersphere support vector machine is a new algorithm in pattern recognition. By studying three kinds of hypersphere support vector machines, it is found that their solutions are identical and the margin between two classes of samples is zero or is not unique. In this letter, a new kind of hypersphere support vector machine is proposed. By introducing a parameter n(n>1), a unique solution of the margin can be obtained. Theoretical analysis and experimental results show that the proposed algorithm can achieve better generaliza-tion performance. 展开更多
关键词 Hypersphere support vector machine MARGIN Generalization performance
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Magnets, children and the bowel: A dangerous attraction? 被引量:2
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作者 Anil Thomas George Sandeep Motiwale 《World Journal of Gastroenterology》 SCIE CAS CSCD 2012年第38期5324-5328,共5页
Reports of magnet ingestion are increasing rapidly globally. However, multiple magnet ingestion, the subsequent potential complications and the importance of the early identification and proper management remain both ... Reports of magnet ingestion are increasing rapidly globally. However, multiple magnet ingestion, the subsequent potential complications and the importance of the early identification and proper management remain both under-recognized and underestimated. Published literature on such cases could possibly represent only the tip of an iceberg with press reports, web blogs and government documents highlighting further occurrence of many more such incidents. The increasing number of complications worldwide being reported secondary to magnet ingestion point not only to an acute lack of awareness about this condition among the medical profession but also among parents and carers who will be in most cases the first to pick up on magnet ingestion. There still seems to be no consensus on the management of magnet ingestion with several algorithms being proposed for management. Prevention of this condition remains a much better option than cure. Proper education and improved awareness among parents and carers and frontline medical staff is key in addressing this rapidly emerging problem. The goal of managing such cases of suspected magnet ingestion should be aimed at reducing delays between ingestion time, diagnosis time and intervention time. 展开更多
关键词 Multiple magnet ingestion Children Bowel injury Fistulation Necrosis
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Study on effects of environmental temperature on dynamic characteristics of Taizhou Yangtze River Bridge 被引量:1
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作者 Miao Changqing Chen Liang Feng Zhaoxiang 《Engineering Sciences》 EI 2011年第2期78-82,92,共6页
The dynamic characteristics of three-tower and two-span suspension bridge are analyzed at different global temperatures. An equivalent cable inner force method is proposed to consider temperature effects and to study ... The dynamic characteristics of three-tower and two-span suspension bridge are analyzed at different global temperatures. An equivalent cable inner force method is proposed to consider temperature effects and to study the effects of environmental temperature on dynamic characteristics of Taizhou Yangtze River Bridge. The result demonstrates that the effects of temperature can not be neglected in static or dynamic analysis of Taizhou Yangtze River Bridge. The relationship between temperature and frequency is negative. The effects of temperature should be taken into account in experimental modal analysis of long-span bridges and damage identification. 展开更多
关键词 dynamic characteristics suspension bridge TEMPERATURE natural frequency damage identification
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Preparation, Spectral Identification and Analytical Studies of Some Transition Metal Complexes with New Thiazolylazo Ligand and Their Biological Activity Study
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作者 Khalid J. AL-Adilee Dunya Yo. Fanfon 《Journal of Chemistry and Chemical Engineering》 2012年第11期1016-1028,共13页
A new MeTAMP (thiazolylazo dye ligand 2-[2-(5-methyl thiazolyl)azo]-4-methoxy phenol) was prepared by coupling reaction between 5-methyl thiazolylazonium chloride with 4-methoxy phenol in alkaline alcoholic soluti... A new MeTAMP (thiazolylazo dye ligand 2-[2-(5-methyl thiazolyl)azo]-4-methoxy phenol) was prepared by coupling reaction between 5-methyl thiazolylazonium chloride with 4-methoxy phenol in alkaline alcoholic solution. The structure of azo dye ligand has been characterized by available technique such as 1H-NMR, mass spectrum, FT-IR, UV-vis and elemental analysis. Eight new chelate complexes of Cr(III), Mn(II), Fe(II), Fe(III), Co(III), Ni(II), Cu(II) and Zn(II) ions were prepared and identified by elemental analysis, atomic absorption, magnetic susceptibility, molar conductance measurements, FT-IR and electronic spectra. The spectral and analytical data show that the ligand a tridentate and coordinating with metal ions through the phenolate O, azo N atom which is the farthest of thiazole molecule and with thiazole N atom. The molar ratio (M:L) was also studied which was found 1:1 for Zn(II) and 1:2 for the rest metal ions. Conductivity measurements for prepared complexes showed 1:1 electrolyte for Cr(III), Fe(IlI), and Co(llI) complexes and non-electrolyte for rest complexes. Stability constants of complexes were calculated by spectrophotometry. Octahedral configuration suggested for Cr(III), Mn(II), Fe(II), Fe(III), Co(III), Ni(II) and Cu(II) but tetrahedral for Zn(IlI) complex. The solid complexes are found to have the general formula IML2]Cl H2O where M = Cr(III), Fe(III) and Co(IIl) and [ML2] H2O where M = Mn(II), Fe(II), Ni(II) and Cu(II) while [MLCI].H20 for Zn(II)-complex. The biological activity of ligand and its complexes against five types of bacteria, Strepto coccus and Staphylococcus aureus as example of gram positive, Pseudomonas, Proteus and Esherichia coli as example of gram negative bacteria by agar plate different technique was studied. The activity was also assayed on molecular level by using PCR in an attempt to monitor DNA banding pattern. 展开更多
关键词 Thaizolyazo ligand coupling reaction metal complexes characterization biological activities.
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The trade network of the Belt and Road Initiative and its topological relationship to the global trade network 被引量:12
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作者 宋周莺 车姝韵 杨宇 《Journal of Geographical Sciences》 SCIE CSCD 2018年第9期1249-1262,共14页
Unimpeded trade is one of the cooperation priorities in the Belt and Road Initiative proposed by China. On 15 May 2017, the Joint Communique of the Leaders Roundtable of the Belt and Road Forum for International Coope... Unimpeded trade is one of the cooperation priorities in the Belt and Road Initiative proposed by China. On 15 May 2017, the Joint Communique of the Leaders Roundtable of the Belt and Road Forum for International Cooperation reaffirmed the participants' shared commitment to build an open economy and ensure free and inclusive trade. The Belt and Road Initiative (BRI) is not only China's new action to drive its open and global develoPment, but also a platform for an increasing number of countries to explore free and inclusive trade and promote a universal, rule-based, open, non-discriminatory, and equitable multilateral trade system. It is therefore important to examine BRI and global trade networks. More specifically, the topological relationship between the this article first analyzes the community structure of trade networks using a community detection algorithm, and then estimates the topological relationship between different trade communities. The findings of this article are as follows. First, this research identified three trade communities and two sub-communities in the BRI trade network, in which China is the core, Russia is the sub-core of the biggest trade community, and India, United Arab Emirates, and Saudi Arabia are cores of the second trade community (South Asia-West Asia). Second, it identified five trade communities in the global trade network, centred on China, USA, Russia, India-United Arab Emirates, and Ger- many-Netherlands-France-Britain and other European developed countries. Third, the topo- logical analysis indicated that in the global trade network, most BRI countries are attracted by the core nodes of the BRI regions, such as the China, Russia and India-United Arab Emirates core nodes, and have strong trade contacts with BRI countries. Most Central-East European countries are mainly attracted by Germany-Netherlands-France-Britain and other developed European countries with a low penetration of BRI trade. Although some Southeast Asian countries are incorporated into the Asia-Australia-South Africa community with China as the core, they still need to strengthen trade linkages with BRI countries. 展开更多
关键词 Belt and Road Initiative global trade network trade community topological relationship
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Metagenome-based analysis:A promising direction for plankton ecological studies 被引量:4
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作者 YAN QingYun YU YuHe 《Science China(Life Sciences)》 SCIE CAS 2011年第1期75-81,共7页
The plankton community plays an especially important role in the functioning of aquatic ecosystems and also in biogeochemical cycles. Since the beginning of marine research expeditions in the 1870s, an enormous number... The plankton community plays an especially important role in the functioning of aquatic ecosystems and also in biogeochemical cycles. Since the beginning of marine research expeditions in the 1870s, an enormous number of planktonic organisms have been described and studied. Plankton investigation has become one of the most important areas of aquatic ecological study, as well as a crucial component of aquatic environmental evaluation. Nonetheless, traditional investigations have mainly focused on morphospecies composition, abundances and dynamics, which primarily depend on morphological identification and counting under microscopes. However, for many species/groups, with few readily observable characteristics, morphological identification and counting have historically been a difficult task. Over the past decades, microbiologists have endeavored to apply and extend molecular techniques to address questions in microbial ecology. These culture-independent studies have generated new insights into microbial ecology. One such strategy, metagenome-based analysis, has also proved to be a powerful tool for plankton research. This mini-review presents a brief history of plankton research using morphological and metagenome-based approaches and the potential applications and further directions of metagenomic analyses in plankton ecological studies are discussed. The use of metagenome-based approaches for plankton ecological study in aquatic ecosystems is encouraged. 展开更多
关键词 METAGENOME plankton community MORPHOSPECIES molecular microbial ecology
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