猪丹毒的发生、诊断和防控

猪丹毒是生猪养殖过程中常见急性传染性疾病,主要是由于病猪感染红斑丹毒丝菌所致,其属于细菌性疾病。自猪丹毒被首次检出后,已经波及到世界各地的生猪养殖行业,给生猪养殖行业带来了巨大损失,而且随着病菌的持续变异,今后病菌防控工作也将更加艰难。文章简要介绍了猪丹毒的概述,从病理学、病原学角度分别阐述了猪丹毒的特点以及类别,同时介绍疾病临床症状以及多种技术诊断方式,以帮助养殖户正确医治和预防猪丹毒。

Clinical research progress of ischemic heart failure and prospect of traditional Chinese medicine

As one of the most common types of heart failure, the diagnosis and treatment of Ischemic heart failure are the main focus of clinic. In recent years, the studies on biomarkers have provided supports for clinical diagnosis, prognosis evaluation and stratification. The clinical application of new drugs and the development of non-drug treatment methods have brought more choices conventional treatment. At the same time, under the guidance of Traditional Chinese Medicine syndrome differentiation theory, Traditional Chinese Medicine therapy also plays an increasingly important role and shows its broad prospects .

MY UNDERSTANDINGS ABOUT ACUPUNCTURE TREATMENT OF LUMBAGO ACCORDING TO SYNDROME-DIFFERENTIATION

In the present paper, the author introduces his individual experience and understandings about acupuncture treatment of lumbago from ① pathogenic causes and pathogenesis, ② syndrome differentiation and analysis, ③ therapeutic methods, ④ treatment according to differentiation of syndromes, and ⑤ key points about treatment of lumbago. The author stresses that in the treatment of lumbago, never forget reinforcing the kidney and treat both the primary and secondary aspects of the disease at the same time.

Logical thinking in pattern differentiation of Traditional Chinese Medicine

OBJECTIVE: To discuss the application of logic to pattern differentiation for treatment in Traditional Chinese Medicine (TCM). METHODS: Comparing logic reasoning of syllogism with the logical thinking ofTCM pattern differentiation for treatment. RESULTS:TCM logical thinking depends on symbolic and intuitive judgment with abstractive reasoning integrated into the process. Although it lacks quantitative measurement, it pays great attention to the comprehensive analysis of a disease's cause and its development patterns to get insight into the essence of illness. CONCLUSION: TCM diagnosis reasoning methodmay lack rigorousness, continuity, systematic induction and deduction, but its logical thinking still can attain its goal following a process with rigorous, regulated and scientific formal logic.

Glass capillary nanopore for single molecule detection

Single molecule detection based on nanopore technology is a very promising approach for medical diagnostics, drug therapy and even DNA sequencing. Compared with other biological nanopores and solid-state nanopores, the glass capillary nanopore has low cost, easy availability and stable mechanical characteristics, thus it has been widely used in the nanopore technology for single molecule detection. In this review, we will focus on the studies of the glass nanopore sensors. The popular glass nanopore fabrication methods would be introduced, and the applications of glass nanopores in the detection of nanoparticles, proteins and DNA molecules would be presented. We hope this review will help widen field of vision and promote the development of the nanopore technology based on the glass capillary nanopores.

Dawn of ocular gene therapy:implications for molecular diagnosis in retinal disease

Personalized medicine aims to utilize genomic information about patients to tailor treatment. Gene replacement therapy for ra- re genetic disorders is perhaps the most extreme form of personalized medicine, in that the patients＇ genome wholly determines their treatment regimen. Gene therapy for retinal disorders is poised to become a clinical reality. The eye is an optimal site for gene therapy due to the relative ease of precise vector delivery, immune system isolation, and availability for monitoring of any potential damage or side effects. Due to these advantages, clinical trials for gene therapy of retinal diseases are currently underway. A necessary precursor to such gene therapies is accurate molecular diagnosis of the mutation（s） underlying disease. In this review, we discuss the application of Next Generation Sequencing （NGS） to obtain such a diagnosis and identify disease causing genes, using retinal disorders as a case study. After reviewing ocular gene therapy, we discuss the application of NGS to the identification of novel Mendelian disease genes. We then compare current, array based mutation detection methods against next NGS-based methods in three retinal diseases： Leber＇s Congenital Amaurosis, Retinitis Pigmentosa, and Stargardt＇s disease. We conclude that next-generation sequencing based diagnosis offers several advantages over array based methods, including a higher rate of successful diagnosis and the ability to more deeply and efficiently assay a broad spectrum of mutations. However, the relative difficulty of interpreting sequence results and the development of standardized, reliable bioinforrnatic tools remain outstanding concerns. In this review, recent advances NGS based molecular diagnoses are discussed, as well as their implications for the development of personalized medicine.

Traditional Chinese Medicine diagnoses in persons with ketamine abuse

OBJECTIVES： To explore the distribution of TCM patterns in ketamine users and the inter-rater reli- ability of TCM diagnosis among TCM practitioners. METHODS： Eighty-four subjects recruited from sub- stance abuse clinics and non-governmental coun- seling services catering for ketamine abusers were examined by one or two TCM practitioners. The dis- tribution and inter-rater reliability of the TCM diag- noses between the two TCM practitioners were de- scribed and measured. RESULTS： Seven TCM patterns were differentiated. The four most common patterns were the Heart Yin deficiency （29.8%）, Kidney Yang deficiency （26.2%）, Kidney Yin deficiency （19.0%）, and Spleen Yang defi-ciency （14.3%）. The agreement between the two TCM practitioners was 59%, the kappa for the three most common patterns was 0.472 （P=O.O03）. The proportion of men was lower in the Kidney Yin defi- ciency group （18.8%）, in comparison to the Heart Yin deficiency （60.0%, P=0.009） and Kidney Yang de- ficiency group （59.1%, P=0.013）. CONCLUSION： The differences between the TCM groups in terms of the source of referral and num- ber of drug uses other than ketamine were of bor- derline significance. Further research should focus on validating diagnostic tools and improving in- ter-rater reliability in TCM to enhance the quality of clinical trials of TCM treatments for ketamine abuse.

Impact of classic massage on blood pressure in patients with clinically diagnosed hypertension

OBJECTIVE: To assess the impact of classic massage on the changes in the values of blood pressure in women with previously diagnosed hypertension.METHODS: The study involved a group of ten women aged 60-68, who had previously been diagnosed with hypertension. Ten sessions of classic massage of the lower limbs were performed on the subjects. The massage sessions were conducted every day over ten consecutive days. Throughout the treatment, the subjects' blood pressure was measured using a manometer with a stethoscope TRO-CARDIO KIT 2 MONO. Their blood pressure was taken 1 min before the massage, as well as 1 min and5 min after each session. The study took place between March and June 2013 in Szpital Specjalistyczny im. J?drzeja ?niadeckiego(J?drzej ?niadecki Specialist Hospital) in Nowy S?cz, at the Department of Internal Diseases.RESULTS: For ten consecutive days, the blood pressure values in the examined women were decreasing, with the exception of the diastolic blood pressure measured 5 min after the massage.CONCLUSION: The drops in blood pressure by several percent, noted in the majority of the female subjects over the time of the study. Classic massage might provide a safe supportive measure in pharmacologic treatment of hypertension.