As great progress has been made in the field of protection of new plant varieties, more attention is paid to the standardization of DUS(Distinctness, Uniformity, and Stability) test procedure. For further studies of t...As great progress has been made in the field of protection of new plant varieties, more attention is paid to the standardization of DUS(Distinctness, Uniformity, and Stability) test procedure. For further studies of tropical plants as their importance in agriculture and germplasm, protection of coconut becomes more significant and thus DUS test technique of coconut is needed. In this essay, we analyzed the status quo of the DUS test guidelines by International Union for the Protection of New Varieties of Plants(UPOV proj.3) and national DUS test guidelines in China, and provided some suggestions or promotions for improving the guidelines of DUS test in coconut.展开更多
The specific recombinant proteins rOspA B. afzelii, rOspA B. garinii a rOspA B. burgdorferi sensu stricto were used as antigens for construction of ELISA sets. ELISA examination enables determination of specific post-...The specific recombinant proteins rOspA B. afzelii, rOspA B. garinii a rOspA B. burgdorferi sensu stricto were used as antigens for construction of ELISA sets. ELISA examination enables determination of specific post-vaccination antibodies against OspA B. afzelii, B. garinii a B. burgdorferi sensu stricto.Using recombinant DNA technology, genes from B. afzelii, B. garinii and B. burgdorferi sensu stricto were inserted into E. coli-expression vectors and the rOspA proteins were produced. These proteins were used for the construction of ELISA kits for the determination of post-vaccination antibodies against individual Borrelia serovars contained in the vaccine. The antibody response of dogs vaccinated with whole-cell vaccine BORRELYM 3 and non-vaccinated dogs was monitored and compared. The ELISA method proved as highly sensitive for the determination of post-vaccination antibodies against individual Borrelia serovars in vaccinated animals. Detection of these antibodies and their quantification may be used for evaluation of efficiency of vaccines against Lyme borreliosis in dogs caused by Borrelia burgdorferi sensu lato. Prospectively, it will be necessary to establish a correlation between post-vaccination antibody levels and protective immunity of vaccinated dogs.展开更多
For the purpose of improving the defects of the conventional friction welding method, the new friction welding technology has been examined. That is, the aim of the study is producing the joint of dissimilar materials...For the purpose of improving the defects of the conventional friction welding method, the new friction welding technology has been examined. That is, the aim of the study is producing the joint of dissimilar materials evaluated to be difficult for friction welding and non-round shape joints. In this process, after the intermediate material generates the independent friction heat on every side of the specimens, it is removed instantaneously and upset process begins to weld the specimens for a joint. In this study, similar joint of A2017 aluminum alloy and one of S45C steel were examined. On the other hand, thermal elastic-plastic stress analysis by the finite element method was carried out using ANSYS mechanical.展开更多
基金Supported by Special Fund for Scientific Research in the Public Interest of Ministry of Agriculture(200903008-15)
文摘As great progress has been made in the field of protection of new plant varieties, more attention is paid to the standardization of DUS(Distinctness, Uniformity, and Stability) test procedure. For further studies of tropical plants as their importance in agriculture and germplasm, protection of coconut becomes more significant and thus DUS test technique of coconut is needed. In this essay, we analyzed the status quo of the DUS test guidelines by International Union for the Protection of New Varieties of Plants(UPOV proj.3) and national DUS test guidelines in China, and provided some suggestions or promotions for improving the guidelines of DUS test in coconut.
文摘The specific recombinant proteins rOspA B. afzelii, rOspA B. garinii a rOspA B. burgdorferi sensu stricto were used as antigens for construction of ELISA sets. ELISA examination enables determination of specific post-vaccination antibodies against OspA B. afzelii, B. garinii a B. burgdorferi sensu stricto.Using recombinant DNA technology, genes from B. afzelii, B. garinii and B. burgdorferi sensu stricto were inserted into E. coli-expression vectors and the rOspA proteins were produced. These proteins were used for the construction of ELISA kits for the determination of post-vaccination antibodies against individual Borrelia serovars contained in the vaccine. The antibody response of dogs vaccinated with whole-cell vaccine BORRELYM 3 and non-vaccinated dogs was monitored and compared. The ELISA method proved as highly sensitive for the determination of post-vaccination antibodies against individual Borrelia serovars in vaccinated animals. Detection of these antibodies and their quantification may be used for evaluation of efficiency of vaccines against Lyme borreliosis in dogs caused by Borrelia burgdorferi sensu lato. Prospectively, it will be necessary to establish a correlation between post-vaccination antibody levels and protective immunity of vaccinated dogs.
文摘For the purpose of improving the defects of the conventional friction welding method, the new friction welding technology has been examined. That is, the aim of the study is producing the joint of dissimilar materials evaluated to be difficult for friction welding and non-round shape joints. In this process, after the intermediate material generates the independent friction heat on every side of the specimens, it is removed instantaneously and upset process begins to weld the specimens for a joint. In this study, similar joint of A2017 aluminum alloy and one of S45C steel were examined. On the other hand, thermal elastic-plastic stress analysis by the finite element method was carried out using ANSYS mechanical.
