试重组迁移矩阵及现场混合动平衡方法

针对全息动平衡方法在现场应用中可能存在因试重起车次数受限、无法获得足够的转子特征响应参数的问题,提出了一种基于试重组的全息现场混合动平衡方法.借鉴Darlow混合法中模态试重组的思想,通过对模态试重比的简化假设,利用迁移矩阵的线性可叠加性将对应于某单一平衡面的迁移矩阵扩展为对应于某阶模态试重组的迁移矩阵,从而减少了平衡高阶不平衡量所需的试重起车次数,提高了现场平衡效率,且平衡效果良好.通过对一台300 MW汽轮发电机组的现场动平衡过程的试验分析,表明了该方法的可行性和有效性.

基于试重组的影响系数动平衡算法

为了充分利用现场动平衡中施加重量组所得的振动数据,采用短阵的方法推导出基于试重组的影响系数动平衡计算方法,并进行了实验验证。实验数据表明该算法行之有效。

Study on DNA Immunization by Recombinants Encoding Japanese Encephalitis Virus prME and E Proteins

To study the expression characteristic of Japanese encephalitis virus (JEV) prME and E proteins and the efficacy of DNA immunization by different recombinant plasmids containing JEV prME (2001 bp) and E (1500 bp) genes, two recombinants (pJME and pJE) containing JEV prME and E genes fused with FLAG were constructed and then transfected into HepG2 and COS-1 cells by liposome fusion. The expression feature of FLAG-prME (about 72 kDa) and FLAG-E (about 54 kDa) proteins in transfected cells were analyzed by Western blot and two antibody systems (anti-FLAG and anti-E). BALB/c mice were immunized with 100 μg of two kinds of recombinants by intramuscular injection, and JEV JaGAr-01 strains (10 5 PFU/100 μl)were given to BALB/c mice by intraperioneal injection 3 wk after twice DNA immunization by a lethal virus challenge. BALB/c mice were observed for 21 days after challenge. 80% plaque reduction neutralization test was performed to titrate neutralization antibody before and after viral challenge. It was found that the expression of proteins associated with pJME and pJE was determined in transfected cells with anti-FLAG and a new protein of 11 kDa was detected in HepG2 and COS-1 cells transfected with pJME. Only E (53 kDa) protein was identified as transfected with pJME using anti-E. Higher level of neutralization antibodies and the efficacy of protective immunity were induced with pJME immunization, and were similar to those induced by inactivated Japanese encephalitis vaccine, but were better than those induced with pJE. It concludes that the expression level from prM to E proteins of JEV is different in vitro, and the in vitro expression efficiency of pJME was better than that of pJE. FLAG-prME protein expressed by pJME could be cleaved by peptidase from host. The efficacy of DNA immunization is correlated to the expression characterization of related proteins expressed in vitro.

Undrained response of reconstituted clay to cyclic pure principal stress rotation

A series of monotonic and rotational shearing tests are carried out on reconstituted clay using a hollow cylinder apparatus under undrained condition. In the rotational shearing tests, the principal stress axes rotate cyclically with the magnitudes of the principal stresses keeping constant. The anisotropy of the reconstituted clay is analyzed from the monotonic shearing tests. Obvious pore pressure is induced by the principal stress rotation alone even with shear stress q0=5 k Pa. Strain components also accumulate with increasing the number of cycles and increases suddenly at the onset of failure. The deviatoric shear strain of 7.5% can be taken as the failure criterion for clay subjected to the pure cyclic principal stress rotation. The intermediate principal stress parameter b plays a significant role in the development of pore pressure and strain. Specimens are weakened by cyclic rotational shearing as the shear modulus decreases with increasing the number of cycles, and the shear modulus reduces more quickly with larger b. Clear deviation between the directions of the principal plastic strain increment and the principal stress is observed during pure principal stress rotation. Both the coaxial and non-coaxial plastic mechanisms should be taken into consideration to simulate the deformation behavior of clay under pure principal stress rotation. The mechanism of the soil response to the pure principal stress rotation is discussed based on the experimental observations.

Intratracheal administration of recombinant adenovirus containing IL-18 gene in treatment of experimental metastatic lung carcinoma

Objective: To study the treatment of experimental metastatic lung carcinoma by intratracheal injection of IL-l8 gene recombinant adenovirus. Methods: (1)The mouse IL-18 mRNA was detected by RT-PCR, and the concentration of IL-18 and associated cytokines in lung lavages and blood were determined by ELISA at different time points after intratracheal injection of IL-18 recombinant adenovirus. (2)The lung metastasis nodes, mouse survival periods and survival rates were evaluated. NK activity and CTL activity were determined by 51Cr 4 h release method. Results: (1) IL-18 mRNA was detectable in lung tissue 6 h after intratracheal use of IL-18 recombinant adenovirus. and the concentration of IL-18 in lung lavage was higher than that in peripheral blood. Neither IL-18 mRNA nor IL-18 was detectable in control group. (2) Intratracheal use of IL-18 recombinant adenovirus resulted in increased CTL and NK activity, longer survival time and higher survival rates compared with the control group, showing significant therapeutic effect on expermental lung metastasis. Conclusion: Intratracheal use of adenovirus vector containing IL- 18 gene has therapeutic effect on the lung metastasis, denoting that gene therapy of lung diseases could be applied through airway directly with recombinant adenovirus.

Determination of Postvaccinal Antibodies Against OspA B. afzelii, B. garinfi and B. burgdorferi sensu stricto Using Elisa Method

The specific recombinant proteins rOspA B. afzelii, rOspA B. garinii a rOspA B. burgdorferi sensu stricto were used as antigens for construction of ELISA sets. ELISA examination enables determination of specific post-vaccination antibodies against OspA B. afzelii, B. garinii a B. burgdorferi sensu stricto.Using recombinant DNA technology, genes from B. afzelii, B. garinii and B. burgdorferi sensu stricto were inserted into E. coli-expression vectors and the rOspA proteins were produced. These proteins were used for the construction of ELISA kits for the determination of post-vaccination antibodies against individual Borrelia serovars contained in the vaccine. The antibody response of dogs vaccinated with whole-cell vaccine BORRELYM 3 and non-vaccinated dogs was monitored and compared. The ELISA method proved as highly sensitive for the determination of post-vaccination antibodies against individual Borrelia serovars in vaccinated animals. Detection of these antibodies and their quantification may be used for evaluation of efficiency of vaccines against Lyme borreliosis in dogs caused by Borrelia burgdorferi sensu lato. Prospectively, it will be necessary to establish a correlation between post-vaccination antibody levels and protective immunity of vaccinated dogs.

Research on a Rapid Test Method of Dry Basis Weight of Paper-process Reconstituted Tobacco

This study aimed to optimize the rapid test factors of dry basis weight of reconstituted tobacco, in order to afford a reference test method for companies which produce reconstituted tobacco to better control the basis weight and coating ratio on line. The dry basis weight of reconstituted tobacco was tested by fast method and normal oven method individually. And the effects on the test values of different test factors such as temperature, time and the number of baking sheets were studied. Then the test values of these two methods were compared, so the proper factors of rapid test method were determined. As the baking temperature rose from 130 ℃ to 150 ℃, and the baking time rose from 1 min to 2 min, the difference between fast test method and normal oven method grew, and when the number of baking pieces rose from 3 pieces to 5 pieces, the difference between the two methods went down. The optimum test condition was baking temperature of 130 ℃, baking time of 1 min, and baking sample sheet number of 5. Under this condition, the value of fast test method was the closest to the test value of normal oven method, and meanwhile, the test factor was more proper for testing on line. The study will provide a reference for online controlling of dry basis weight and coating ratio of reconstituted tobacco.