The H_∞ performance analysis and controller design for linear networked control systems(NCSs) are presented.The NCSs are considered a linear continuous system with time-varying interval input delay by assuming that t...The H_∞ performance analysis and controller design for linear networked control systems(NCSs) are presented.The NCSs are considered a linear continuous system with time-varying interval input delay by assuming that the sensor is time-driven and the logic Zero-order-holder(ZOH) and controller are event-driven.Based on this model,the delay interval is divided into two equal subintervals for H_∞ performance analysis.An improved H_∞ stabilization condition is obtained in linear matrix inequalities(LMIs) framework by adequately considering the information about the bounds of the input delay to construct novel Lyapunov–Krasovskii functionals(LKFs).For the purpose of reducing the conservatism of the proposed results,the bounds of the LKFs differential cross terms are properly estimated without introducing any slack matrix variables.Moreover,the H_∞ controller is reasonably designed to guarantee the robust asymptotic stability for the linear NCSs with an H_∞ performance level γ.Numerical simulation examples are included to validate the reduced conservatism and effectiveness of our proposed method.展开更多
In this paper,the fault detection filter(FDF) design problem for networked control systems(NCSs) with both network-induced delay and data dropout is studied.Based on a new NCSs model proposed recently,an observer-base...In this paper,the fault detection filter(FDF) design problem for networked control systems(NCSs) with both network-induced delay and data dropout is studied.Based on a new NCSs model proposed recently,an observer-based filter is introduced to be the residual generator and formulated as an H∞-optimization problem for systems with two successive delay components.By applying Lyapunov-Krasovskii approach,a new sufficient condition on stability and H∞ performance is derived for systems with two successive delay components in the state.A solution of the optimization problem is then presented in terms of linear matrix inequality(LMI) formulation,dependently of time delay.In order to detect the fault,the residual evaluation problem is also considered.An illustrative design example is employed to demonstrate the validity of the proposed approach.展开更多
AIM: To determine if proteasome inhibition induces apoptosis in human cholangiocarcinoma cells, and if so, to elucidate the cellular mechanisms. METHODS: Studies were performed in the human KMCH, KMBC, and Mz-ChA-1 ch...AIM: To determine if proteasome inhibition induces apoptosis in human cholangiocarcinoma cells, and if so, to elucidate the cellular mechanisms. METHODS: Studies were performed in the human KMCH, KMBC, and Mz-ChA-1 cholangiocarcinoma, and normal rat cell lines. MG132, a peptide aldehyde, which inhibits the chymotrypsin-like activity of the proteaosome was employed for this study. Apoptosis was assessed morphologically by 4'-6-Diamidino-2-phenylindole (DAPI) nuclear staining and fluorescence microscopy. Mitochondrial membrane potential was examined using a fluorescent unquenching assay. Ultrastructural changes during cell death were examined using transmission electron microscopy (TEM). Caspase 3/7 activity was assessed using an enzymatic-based fluorescent assay. Cytosolic-free calcium concentrations were measured using Fura-2 and digitized fluorescent microscopy. RESULTS: MG132, a proteasome inhibitor, induced apoptosis in all the cholangiocarcinoma cell lines examined. In contrast, minimal cytotoxicity was observed in normal rat cholangiocytes. Apoptosis was time-and -concentration-dependent. There was no change in the mitochondrial membrane potential between treated and untreated cells. Ultrastructural examination by transmission electron microscopy displayed the classic features of apoptosis, but in addition, there was also dramatic vacuolization of the endoplasmic reticulum (ER). Unexpectedly, no increase in caspase 3/7 activity was observed in MG132 treated cells, nor did the pancaspase inhibitor, Q-VD-OPh prevent cell death. The protein synthesis inhibitor, cycloheximide, blocked apoptosis induced by proteosome inhibitor indicating that ER dysfunction was dependent upon the formation of new proteins. CONCLUSION: Proteosome inhibition induces ERdysfunction and caspase-independent cell death selectively in human cholangiocarcinoma cells. Proteasome inhibitors warrant evaluation as anticancer agents for the treatment of human cholangiocarcinoma.展开更多
Objective: To examine factors of the youth physical activity promotion (YPAP) model that are associated with Chinese international students' meeting PA recommendations (MPAR). Methods: A total of 649 (females ...Objective: To examine factors of the youth physical activity promotion (YPAP) model that are associated with Chinese international students' meeting PA recommendations (MPAR). Methods: A total of 649 (females = 320, males = 329) Chinese international college students participated in the study. This study was conducted in the United States (US.) between June and August in 2011. Participants completed an online survey regarding their demographic, PA, predisposing, enabling, and reinforcing factors. Results: Logistic nested-regression analysis suggested that being male, having a higher body mass index, perceived competence, self-efficacy, attitude, and enjoyment all increased (p 〈 0.001) the participants' odds of MPAR. Except language barriers, all of the enabling and reinforcing factors predicted MPAR indirectly through the predisposing factors (p 〈 0.001). Conclusion: Understanding factors that influence PA among Chinese international students is an important step in the process of promoting their long-term health and wellbeing. Designing program that address the identified key factors may help colleges and universities achieve this goal.展开更多
A new method of modeling discrete networked control systems with both the time-varying delay and packet loss was presented in this paper.The network-induced delay is time-varying and it can be smaller or larger than o...A new method of modeling discrete networked control systems with both the time-varying delay and packet loss was presented in this paper.The network-induced delay is time-varying and it can be smaller or larger than one sampling period.The feedback controller was designed by free-weighting matrices method and Lyapunov-Krasovskii functional to make the networked control system asymptotically stable.And sufficient conditions were derived.A numerical example is given to illustrate the effectiveness of the proposed approach.展开更多
According to the construction project of the crustal deformation mobile monitoring network in the cascade hydropower stations built in the lower reaches of Jinsha River,this paper analyzes the design ideas and layout ...According to the construction project of the crustal deformation mobile monitoring network in the cascade hydropower stations built in the lower reaches of Jinsha River,this paper analyzes the design ideas and layout principles of crustal deformation mobile monitoring used in the monitoring of reservoir induced earthquakes. This paper introduces three types of monitoring networks used in the Xiluodu reservoir and Xiangjiaba reservoir, as well as the work already undertaken,in order to provide a kind of reference for the related engineering construction and comprehensive monitoring of reservoir induced earthquakes.展开更多
Macrophage cells play an important role in the initiation and regulation of the immune response. All-trans retinoic acid (ATRA) and its natural and synthetic analogs (retinoids) affect a large number of biological pro...Macrophage cells play an important role in the initiation and regulation of the immune response. All-trans retinoic acid (ATRA) and its natural and synthetic analogs (retinoids) affect a large number of biological processes.Recently , retinoids have been shown promise in the therapy and prevention of various cancers. However, many interesting questions related to the activities of retinoids remain to be answered: (Ⅰ) Molecular mechanisms by which retinoids exert their effects; (Ⅱ) why the clinical uses of retinoids give undesirable side effects of varying severity with a higher freqllency of blood system symptoms; (Ⅲ)little is known for its impacts on macrophage cells etc. We set up this experiment, therefore, to examine the apoptosis of ATRA on macrophage Ana-1 cell line. Apoptosis of the cells was quantitated, after staining cells with propidium iodide (PI), by both accounting nuclear condensation and flow cytometry. When the cells were treated with ATRA at or higher than 1 μM for more than 24 h, significant amount of the apoptotic cells was observed. Induction of apoptosis of Ana-1 cells by ATRA was in time- and dose-dependent manners, exhibiting the similar pattern as the apoptosis induced by actinomycin D (ACTD). ATRA treatment of Ana-1 cells also caused the changes of the mRNA levels of apoptosis-associated gene bcl-2, as detected by Northern blot analysis. The temporal changes of bcl-2 expression by ATRA was also parallel to that by ACTD. In conclusion,ATRA can induce apoptosis in macrophage cells, which may be helpful in understanding of immunological functions retinoids.展开更多
Despite the tremendous effort made by industry and academia,we are still searching for metrics that can characterize Cyberspace and system security risks. In this paper,we study the class of security risks that are in...Despite the tremendous effort made by industry and academia,we are still searching for metrics that can characterize Cyberspace and system security risks. In this paper,we study the class of security risks that are inherent to the dependence structure in software with vulnerabilities and exhibit a "cascading" effect. We present a measurement framework for evaluating these metrics,and report a preliminary case study on evaluating the dependence-induced security risks in the Apache HTTP Server. The experiment results show that our framework can not only clearly analyze the root cause of the security risks but also quantitatively evaluate the attack consequence of the risks.展开更多
基金Project (61304046) supported by the National Natural Science Funds for Young Scholar of ChinaProject (F201242) supported by Natural Science Foundation of Heilongjiang Province,China
文摘The H_∞ performance analysis and controller design for linear networked control systems(NCSs) are presented.The NCSs are considered a linear continuous system with time-varying interval input delay by assuming that the sensor is time-driven and the logic Zero-order-holder(ZOH) and controller are event-driven.Based on this model,the delay interval is divided into two equal subintervals for H_∞ performance analysis.An improved H_∞ stabilization condition is obtained in linear matrix inequalities(LMIs) framework by adequately considering the information about the bounds of the input delay to construct novel Lyapunov–Krasovskii functionals(LKFs).For the purpose of reducing the conservatism of the proposed results,the bounds of the LKFs differential cross terms are properly estimated without introducing any slack matrix variables.Moreover,the H_∞ controller is reasonably designed to guarantee the robust asymptotic stability for the linear NCSs with an H_∞ performance level γ.Numerical simulation examples are included to validate the reduced conservatism and effectiveness of our proposed method.
基金National Natural Science Foundation of China(No.60574081)
文摘In this paper,the fault detection filter(FDF) design problem for networked control systems(NCSs) with both network-induced delay and data dropout is studied.Based on a new NCSs model proposed recently,an observer-based filter is introduced to be the residual generator and formulated as an H∞-optimization problem for systems with two successive delay components.By applying Lyapunov-Krasovskii approach,a new sufficient condition on stability and H∞ performance is derived for systems with two successive delay components in the state.A solution of the optimization problem is then presented in terms of linear matrix inequality(LMI) formulation,dependently of time delay.In order to detect the fault,the residual evaluation problem is also considered.An illustrative design example is employed to demonstrate the validity of the proposed approach.
基金NIH grants DK63947 (to GJG)the Mayo and Palumbo Foundations
文摘AIM: To determine if proteasome inhibition induces apoptosis in human cholangiocarcinoma cells, and if so, to elucidate the cellular mechanisms. METHODS: Studies were performed in the human KMCH, KMBC, and Mz-ChA-1 cholangiocarcinoma, and normal rat cell lines. MG132, a peptide aldehyde, which inhibits the chymotrypsin-like activity of the proteaosome was employed for this study. Apoptosis was assessed morphologically by 4'-6-Diamidino-2-phenylindole (DAPI) nuclear staining and fluorescence microscopy. Mitochondrial membrane potential was examined using a fluorescent unquenching assay. Ultrastructural changes during cell death were examined using transmission electron microscopy (TEM). Caspase 3/7 activity was assessed using an enzymatic-based fluorescent assay. Cytosolic-free calcium concentrations were measured using Fura-2 and digitized fluorescent microscopy. RESULTS: MG132, a proteasome inhibitor, induced apoptosis in all the cholangiocarcinoma cell lines examined. In contrast, minimal cytotoxicity was observed in normal rat cholangiocytes. Apoptosis was time-and -concentration-dependent. There was no change in the mitochondrial membrane potential between treated and untreated cells. Ultrastructural examination by transmission electron microscopy displayed the classic features of apoptosis, but in addition, there was also dramatic vacuolization of the endoplasmic reticulum (ER). Unexpectedly, no increase in caspase 3/7 activity was observed in MG132 treated cells, nor did the pancaspase inhibitor, Q-VD-OPh prevent cell death. The protein synthesis inhibitor, cycloheximide, blocked apoptosis induced by proteosome inhibitor indicating that ER dysfunction was dependent upon the formation of new proteins. CONCLUSION: Proteosome inhibition induces ERdysfunction and caspase-independent cell death selectively in human cholangiocarcinoma cells. Proteasome inhibitors warrant evaluation as anticancer agents for the treatment of human cholangiocarcinoma.
文摘Objective: To examine factors of the youth physical activity promotion (YPAP) model that are associated with Chinese international students' meeting PA recommendations (MPAR). Methods: A total of 649 (females = 320, males = 329) Chinese international college students participated in the study. This study was conducted in the United States (US.) between June and August in 2011. Participants completed an online survey regarding their demographic, PA, predisposing, enabling, and reinforcing factors. Results: Logistic nested-regression analysis suggested that being male, having a higher body mass index, perceived competence, self-efficacy, attitude, and enjoyment all increased (p 〈 0.001) the participants' odds of MPAR. Except language barriers, all of the enabling and reinforcing factors predicted MPAR indirectly through the predisposing factors (p 〈 0.001). Conclusion: Understanding factors that influence PA among Chinese international students is an important step in the process of promoting their long-term health and wellbeing. Designing program that address the identified key factors may help colleges and universities achieve this goal.
基金National Natural Science Foundation of China(No.70701012)Youth Science and Technology Special Fund of Heilongjiang Province of China(No.QC07C15)
文摘A new method of modeling discrete networked control systems with both the time-varying delay and packet loss was presented in this paper.The network-induced delay is time-varying and it can be smaller or larger than one sampling period.The feedback controller was designed by free-weighting matrices method and Lyapunov-Krasovskii functional to make the networked control system asymptotically stable.And sufficient conditions were derived.A numerical example is given to illustrate the effectiveness of the proposed approach.
基金sponsored by the Key Construction Program of the Three Gorges Project,China
文摘According to the construction project of the crustal deformation mobile monitoring network in the cascade hydropower stations built in the lower reaches of Jinsha River,this paper analyzes the design ideas and layout principles of crustal deformation mobile monitoring used in the monitoring of reservoir induced earthquakes. This paper introduces three types of monitoring networks used in the Xiluodu reservoir and Xiangjiaba reservoir, as well as the work already undertaken,in order to provide a kind of reference for the related engineering construction and comprehensive monitoring of reservoir induced earthquakes.
文摘Macrophage cells play an important role in the initiation and regulation of the immune response. All-trans retinoic acid (ATRA) and its natural and synthetic analogs (retinoids) affect a large number of biological processes.Recently , retinoids have been shown promise in the therapy and prevention of various cancers. However, many interesting questions related to the activities of retinoids remain to be answered: (Ⅰ) Molecular mechanisms by which retinoids exert their effects; (Ⅱ) why the clinical uses of retinoids give undesirable side effects of varying severity with a higher freqllency of blood system symptoms; (Ⅲ)little is known for its impacts on macrophage cells etc. We set up this experiment, therefore, to examine the apoptosis of ATRA on macrophage Ana-1 cell line. Apoptosis of the cells was quantitated, after staining cells with propidium iodide (PI), by both accounting nuclear condensation and flow cytometry. When the cells were treated with ATRA at or higher than 1 μM for more than 24 h, significant amount of the apoptotic cells was observed. Induction of apoptosis of Ana-1 cells by ATRA was in time- and dose-dependent manners, exhibiting the similar pattern as the apoptosis induced by actinomycin D (ACTD). ATRA treatment of Ana-1 cells also caused the changes of the mRNA levels of apoptosis-associated gene bcl-2, as detected by Northern blot analysis. The temporal changes of bcl-2 expression by ATRA was also parallel to that by ACTD. In conclusion,ATRA can induce apoptosis in macrophage cells, which may be helpful in understanding of immunological functions retinoids.
基金supported by Natural Science Foundation of China under award No.61303024Natural Science Foundation of Jiangsu Province under award No.BK20130372+3 种基金National 973 Program of China under award No.2014CB340600National High Tech 863 Program of China under award No.2015AA016002supported by Natural Science Foundation of China under award No.61272452supported in part by ARO Grant # W911NF-12-1-0286 and NSF Grant #1111925
文摘Despite the tremendous effort made by industry and academia,we are still searching for metrics that can characterize Cyberspace and system security risks. In this paper,we study the class of security risks that are inherent to the dependence structure in software with vulnerabilities and exhibit a "cascading" effect. We present a measurement framework for evaluating these metrics,and report a preliminary case study on evaluating the dependence-induced security risks in the Apache HTTP Server. The experiment results show that our framework can not only clearly analyze the root cause of the security risks but also quantitatively evaluate the attack consequence of the risks.