Northern blot analysis was conducted with mitochondrial RNA from seedling leaves, floral buds, and developing seeds of NCa CMS, maintainer line and fertile F1 using ten mitochondrial genes as probes. The results revea...Northern blot analysis was conducted with mitochondrial RNA from seedling leaves, floral buds, and developing seeds of NCa CMS, maintainer line and fertile F1 using ten mitochondrial genes as probes. The results revealed that 9 out of the 10 mitochondrial genes, except for atp6, showed no difference in different tissues of the corresponding materials of NCα CMS system and that they might be constitutively expressed genes. Eight genes, such as orf139, orf222, atpl, cox1, cox2, cob, rm5S, and rm26S, showed no difference among the three tissues of all the materials detected. So the expression of these eight genes was not regulated by nuclear genes and was not tissue-specific. The transcripts of atp9 were identical among different tissues, but diverse among different materials, indicating that transcription of atp9 was neither controlled by nuclear gene nor tissue-specific. Gene atp6 displayed similar transcripts with the same size among different tissues of all the materials but differed in abundance among tissues of corresponding materials and its expression might be tissue-specific under regulation of nuclear gene. Moreover, three transcripts of orf222 were detected in the floral buds of NCa cms and fertile F1, but no transcript was detected in floral buds of the maintainer line.The transcription of orf139 was similar to that of orf222 but only two transcripts of 0.8 kb and 0.6 kb were produced. The atp9 probe detected a single transcript of 0.6 kb in NCa cms and in maintainer line and an additional transcript of 1.2 kb in fertile F1. The relationship of expression of orf222, orf139, and atp9 with NCa sterility was discussed.展开更多
为了了解草原生态系统氨气挥发情况、历史放牧的影响及其影响机制,2005和2006年生长季期间,在长达17年放牧处理的样地上测定了5个放牧强度下的氨气挥发量。结果表明:氨气挥发随季节变化波动很大,春季和初秋高,晚秋和夏季低,变化范围为0....为了了解草原生态系统氨气挥发情况、历史放牧的影响及其影响机制,2005和2006年生长季期间,在长达17年放牧处理的样地上测定了5个放牧强度下的氨气挥发量。结果表明:氨气挥发随季节变化波动很大,春季和初秋高,晚秋和夏季低,变化范围为0.88-3.52 gN ha<sup>-1</sup>d<sup>-1</sup>。土壤pH值、NH<sub>4</sub><sup>+</sup>-N浓度、湿度以及容重对氨气挥发控制作用较强。但是容重的影响与其他3个因子的影响作用相反,而容重的大小直接受不同放牧强度影响。虽然不同的放牧强度对氨气释放的影响在统计学上来说并不显著,但是,相对不放牧的情况而言,氨气挥发量在放牧情况下更大。而土壤氮浓度、植物生物量却在放牧的情况下更低。这些都意味着放牧草地生态系统比不放牧草地生态系统恢复起来会更慢。这种关于草原生态系统氨气挥发的研究意义重大,因为它有助于加强了解氮素丢失对草原各进程的抑制作用,同时对制订适宜的放牧及施肥措施从而达到合理利用资源的目的也具有重要的参考价值。展开更多
基金This work was supported by the National High Technology R&D Project of China (No.2002AA207009) and Wuhan Dawn Project for Youth (No. 20035002016-36).
文摘Northern blot analysis was conducted with mitochondrial RNA from seedling leaves, floral buds, and developing seeds of NCa CMS, maintainer line and fertile F1 using ten mitochondrial genes as probes. The results revealed that 9 out of the 10 mitochondrial genes, except for atp6, showed no difference in different tissues of the corresponding materials of NCα CMS system and that they might be constitutively expressed genes. Eight genes, such as orf139, orf222, atpl, cox1, cox2, cob, rm5S, and rm26S, showed no difference among the three tissues of all the materials detected. So the expression of these eight genes was not regulated by nuclear genes and was not tissue-specific. The transcripts of atp9 were identical among different tissues, but diverse among different materials, indicating that transcription of atp9 was neither controlled by nuclear gene nor tissue-specific. Gene atp6 displayed similar transcripts with the same size among different tissues of all the materials but differed in abundance among tissues of corresponding materials and its expression might be tissue-specific under regulation of nuclear gene. Moreover, three transcripts of orf222 were detected in the floral buds of NCa cms and fertile F1, but no transcript was detected in floral buds of the maintainer line.The transcription of orf139 was similar to that of orf222 but only two transcripts of 0.8 kb and 0.6 kb were produced. The atp9 probe detected a single transcript of 0.6 kb in NCa cms and in maintainer line and an additional transcript of 1.2 kb in fertile F1. The relationship of expression of orf222, orf139, and atp9 with NCa sterility was discussed.
基金International Science and Technology Partnerships Program of China and Canada(2009DFA91900)the national natural science foundation of China(30700107,30670347,30521002)the State Key Basic Research Development Program of China(2007CB106800)
文摘为了了解草原生态系统氨气挥发情况、历史放牧的影响及其影响机制,2005和2006年生长季期间,在长达17年放牧处理的样地上测定了5个放牧强度下的氨气挥发量。结果表明:氨气挥发随季节变化波动很大,春季和初秋高,晚秋和夏季低,变化范围为0.88-3.52 gN ha<sup>-1</sup>d<sup>-1</sup>。土壤pH值、NH<sub>4</sub><sup>+</sup>-N浓度、湿度以及容重对氨气挥发控制作用较强。但是容重的影响与其他3个因子的影响作用相反,而容重的大小直接受不同放牧强度影响。虽然不同的放牧强度对氨气释放的影响在统计学上来说并不显著,但是,相对不放牧的情况而言,氨气挥发量在放牧情况下更大。而土壤氮浓度、植物生物量却在放牧的情况下更低。这些都意味着放牧草地生态系统比不放牧草地生态系统恢复起来会更慢。这种关于草原生态系统氨气挥发的研究意义重大,因为它有助于加强了解氮素丢失对草原各进程的抑制作用,同时对制订适宜的放牧及施肥措施从而达到合理利用资源的目的也具有重要的参考价值。
