To investigate molecular epidemiology of DuCV in Cherry Valley ducks in China, the complete genomes of six DuCV strains, which were detected from Cherry Valley ducks in China between 2007 and 2008, were sequenced. Seq...To investigate molecular epidemiology of DuCV in Cherry Valley ducks in China, the complete genomes of six DuCV strains, which were detected from Cherry Valley ducks in China between 2007 and 2008, were sequenced. Sequence and phylogenetic analysis were carried out to compare these six strains with another 27 DuCV strains from Mulard duck, Muscovy duck, Pekin ducks and Mule duck. The analysis showed that the six DuCV strains exhibited typical genetic features of the family of DuCV, such as a stem-loop structure, three major open reading frames (Rep, Cap and ORF3), four intergenic repeats and the conserved motifs for rolling circle replication and for the dNTP binding domain located in the Rep protein. Phylogenetic analysis of the nucleotide sequences of the complete genome and Cap gene of these strains together with those that have been previously published demonstrated two distinct DuCV genotypes. The DuCV strains with complete genomes containing 1988 and 1989 nucleotides clustered in genotype A, whereas the strains with complete genomes containing 1991, 1992, 1995 and 1996 nucleotides lay in genotype B. The six DuCV strains from Cherry Valley ducks were divided into the two groups. The results of the study provides some insight into the variation of DuCVs in Cherry Valley ducks.展开更多
Accident at Fukushima Dai-ichi nuclear power plant significantly affected the nuclear industry at time when everybody was expecting the so called nuclear renaissance. There is no question that the accident has at leas...Accident at Fukushima Dai-ichi nuclear power plant significantly affected the nuclear industry at time when everybody was expecting the so called nuclear renaissance. There is no question that the accident has at least slowed it down. Research on this accident is taking place all over the world, in this paper, we present the findings of research on Fukushima nuclear power plant accident in relation to the Czech Republic. The paper focuses on the analysis of human performance during the accident. Lessons learned from the accident and main human errors are presented. First, the brief factors affecting the human performance are discussed. They are followed by the short description of activities on units 1-3. The key human errors in the accident mitigation are then identified. On unit 1, the fuel damage was probably impossible to prevent, however, on units 2 and 3, it could be probably prevented.展开更多
基金the grant of Shandong Province Higher Educational Science and Technology Program (J08LF07)Shandong Provincial Natural Sciences Fund (Q2006D04)
文摘To investigate molecular epidemiology of DuCV in Cherry Valley ducks in China, the complete genomes of six DuCV strains, which were detected from Cherry Valley ducks in China between 2007 and 2008, were sequenced. Sequence and phylogenetic analysis were carried out to compare these six strains with another 27 DuCV strains from Mulard duck, Muscovy duck, Pekin ducks and Mule duck. The analysis showed that the six DuCV strains exhibited typical genetic features of the family of DuCV, such as a stem-loop structure, three major open reading frames (Rep, Cap and ORF3), four intergenic repeats and the conserved motifs for rolling circle replication and for the dNTP binding domain located in the Rep protein. Phylogenetic analysis of the nucleotide sequences of the complete genome and Cap gene of these strains together with those that have been previously published demonstrated two distinct DuCV genotypes. The DuCV strains with complete genomes containing 1988 and 1989 nucleotides clustered in genotype A, whereas the strains with complete genomes containing 1991, 1992, 1995 and 1996 nucleotides lay in genotype B. The six DuCV strains from Cherry Valley ducks were divided into the two groups. The results of the study provides some insight into the variation of DuCVs in Cherry Valley ducks.
文摘Accident at Fukushima Dai-ichi nuclear power plant significantly affected the nuclear industry at time when everybody was expecting the so called nuclear renaissance. There is no question that the accident has at least slowed it down. Research on this accident is taking place all over the world, in this paper, we present the findings of research on Fukushima nuclear power plant accident in relation to the Czech Republic. The paper focuses on the analysis of human performance during the accident. Lessons learned from the accident and main human errors are presented. First, the brief factors affecting the human performance are discussed. They are followed by the short description of activities on units 1-3. The key human errors in the accident mitigation are then identified. On unit 1, the fuel damage was probably impossible to prevent, however, on units 2 and 3, it could be probably prevented.
