AIM: To investigate whether the farnesoid X receptor (FXR) regulates expression of liver cystathionase (CSE), a gene involved in hydrogen sulfi de (H2S) generation. METHODS: The regulation of CSE expression in respons...AIM: To investigate whether the farnesoid X receptor (FXR) regulates expression of liver cystathionase (CSE), a gene involved in hydrogen sulfi de (H2S) generation. METHODS: The regulation of CSE expression in response to FXR ligands was evaluated in HepG2 cells and in wild-type and FXR null mice treated with 6-ethyl chenodeoxycholic acid (6E-CDCA), a synthetic FXR ligand. The analysis demonstrated an FXR responsive element in the 5'-flanking region of the human CSE gene. The function of this site was investigated by luciferase reporter assays, chromatin immunoprecipitation and electrophoretic mobility shift assays. Livers obtained from rats treated with carbon tetrachloride alone, or in combination with 6-ethyl chenodeoxycholic acid, were studied for hydrogen sulphide generation and portal pressure measurement. RESULTS: Liver expression of CSE is regulated by bile acids by means of an FXR-mediated mechanism. Western blotting, qualitative and quantitative polymerase chain reaction, as well as immunohistochemical analysis, showed that expression of CSE in HepG2 cells and in mice is induced by treatment with an FXR ligand. Administration of 6E-CDCA to carbon tetrachloride treated rats protected against the down-regulation of CSE expression, increased H2S generation, reduced portal pressure and attenuated the endothelial dysfunction of isolated and perfused cirrhotic rat livers. CONCLUSION: These results demonstrate that CSE is an FXR-regulated gene and provide a new molecular explanation for the pathophysiology of portal hypertension.展开更多
A field trial was carried out in Ferk6 2 Sugar mill located in northern Ivory Coast, in order to study sugarcane growth and yield response to deficit irrigation imposed over the yield formation stage. The crop used wa...A field trial was carried out in Ferk6 2 Sugar mill located in northern Ivory Coast, in order to study sugarcane growth and yield response to deficit irrigation imposed over the yield formation stage. The crop used was a first ratoon Co 957, a non flowering late season sugarcane variety. The experiment was completely randomized following a one-factor design with 4 water deficit treatments in 3 replicates. It came out that the optimum water deficit treatment reached 20%, i.e., 80% of crop water requirements were satisfied through irrigation. That treatment gave 7.9 kg cane/m3 or 0.98 kg sugar/m3 as irrigation water use efficiency. Relatively low crop growth rates （0.2-0.4 cm/day） as well as low cane yields （24.5-78.4 t/ha） were obtained as a result of an intensive and persistent dry season occurred over the yield formation stage. In line of prevailing climatic conditions, cane juice quality measured was particularly high on Co 957 which used to be a moderately performing variety in Ferk6 2 sugar mill.展开更多
文摘AIM: To investigate whether the farnesoid X receptor (FXR) regulates expression of liver cystathionase (CSE), a gene involved in hydrogen sulfi de (H2S) generation. METHODS: The regulation of CSE expression in response to FXR ligands was evaluated in HepG2 cells and in wild-type and FXR null mice treated with 6-ethyl chenodeoxycholic acid (6E-CDCA), a synthetic FXR ligand. The analysis demonstrated an FXR responsive element in the 5'-flanking region of the human CSE gene. The function of this site was investigated by luciferase reporter assays, chromatin immunoprecipitation and electrophoretic mobility shift assays. Livers obtained from rats treated with carbon tetrachloride alone, or in combination with 6-ethyl chenodeoxycholic acid, were studied for hydrogen sulphide generation and portal pressure measurement. RESULTS: Liver expression of CSE is regulated by bile acids by means of an FXR-mediated mechanism. Western blotting, qualitative and quantitative polymerase chain reaction, as well as immunohistochemical analysis, showed that expression of CSE in HepG2 cells and in mice is induced by treatment with an FXR ligand. Administration of 6E-CDCA to carbon tetrachloride treated rats protected against the down-regulation of CSE expression, increased H2S generation, reduced portal pressure and attenuated the endothelial dysfunction of isolated and perfused cirrhotic rat livers. CONCLUSION: These results demonstrate that CSE is an FXR-regulated gene and provide a new molecular explanation for the pathophysiology of portal hypertension.
文摘A field trial was carried out in Ferk6 2 Sugar mill located in northern Ivory Coast, in order to study sugarcane growth and yield response to deficit irrigation imposed over the yield formation stage. The crop used was a first ratoon Co 957, a non flowering late season sugarcane variety. The experiment was completely randomized following a one-factor design with 4 water deficit treatments in 3 replicates. It came out that the optimum water deficit treatment reached 20%, i.e., 80% of crop water requirements were satisfied through irrigation. That treatment gave 7.9 kg cane/m3 or 0.98 kg sugar/m3 as irrigation water use efficiency. Relatively low crop growth rates （0.2-0.4 cm/day） as well as low cane yields （24.5-78.4 t/ha） were obtained as a result of an intensive and persistent dry season occurred over the yield formation stage. In line of prevailing climatic conditions, cane juice quality measured was particularly high on Co 957 which used to be a moderately performing variety in Ferk6 2 sugar mill.
