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调磷因子:成纤维细胞生长因子23 被引量:11
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作者 夏维波 孟迅吾 周学瀛 《国外医学(内分泌学分册)》 2004年第4期241-243,共3页
低磷性佝偻病/骨软化症的发生与体内的调磷因子有关。目前的研究表明成纤维细胞生长因子(FGF)-23属于体内重要的调磷因子,低磷性佝偻病/骨软化症患者的血循环中FGF-23水平升高。FGF-23可调节钠-磷共转运蛋白Ⅱa(NaPiⅡa)和1α羟化酶的... 低磷性佝偻病/骨软化症的发生与体内的调磷因子有关。目前的研究表明成纤维细胞生长因子(FGF)-23属于体内重要的调磷因子,低磷性佝偻病/骨软化症患者的血循环中FGF-23水平升高。FGF-23可调节钠-磷共转运蛋白Ⅱa(NaPiⅡa)和1α羟化酶的活性从而引起低磷性佝偻病/骨软化症。 展开更多
关键词 调磷因子 成纤维细胞生长因子23 血症 遗传性 佝偻病 骨软化症
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钙调素蛋白磷酸酶对Slingshot-1L的调控作用
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作者 王杨 王岩 +1 位作者 赵建武 高忠礼 《中国老年学杂志》 CAS CSCD 北大核心 2010年第3期353-355,共3页
目的探讨钙调素依赖蛋白激酶(Calcinenurin/PP2B/CnA/Cn)对Slingshot-1L(SSH-1L)活性的调控作用。方法用脂质体法将含YFP-SSH-1L的重组质粒转染至MG63细胞,经钙离子载体A23187诱导10min,进行免疫细胞化学染色,观察细胞形态变化;体内及... 目的探讨钙调素依赖蛋白激酶(Calcinenurin/PP2B/CnA/Cn)对Slingshot-1L(SSH-1L)活性的调控作用。方法用脂质体法将含YFP-SSH-1L的重组质粒转染至MG63细胞,经钙离子载体A23187诱导10min,进行免疫细胞化学染色,观察细胞形态变化;体内及体外实验检测PP3B对SSH-1L的活性调控作用;应用免疫共沉实验检测PP2B与SSH-1L的相互结合情况。结果Ca2+信号诱导骨肉瘤细胞形成伪叶,同时SSH-1L与F-actin移位共聚集至细胞膜伪叶,但是被PP2B有效抑制剂Cypermethrin完全阻断;在体外,SSH-1L磷酸酶活性测定实验以及SSH-1L与PP2B结合实验证实SSH-1L与PP2B在细胞内是一对相互结合的蛋白质,同时PP2B具有促进SSH-1L酶活性的作用。结论PP2B通路对细胞伪叶的形成及细胞运动具有重要调控作用,同时对SSH-1L酶具有促进其活性的作用。 展开更多
关键词 调磷蛋白激酶(Calcineurin/PP2B/Cna/Cn) Slingshot-1L(SSH-1L) 骨肉瘤
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压力负荷下缬沙坦对钙调神经磷酸酶介导心肌肥大通路的影响 被引量:2
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作者 周兴文 李刚 速晓华 《心脏杂志》 CAS 2003年第3期215-217,共3页
目的 :探讨压力超负荷模型下血管紧张素 ( Ang )受体拮抗剂缬沙坦对钙调神经磷酸酶 ( Ca N)介导心肌肥大通路的影响及 Ang 的致心肌肥厚作用。方法 :腹主动脉缩窄法建立大鼠压力超负荷模型 ,放射免疫法检测血浆、心肌 Ang 浓度 ,Wester... 目的 :探讨压力超负荷模型下血管紧张素 ( Ang )受体拮抗剂缬沙坦对钙调神经磷酸酶 ( Ca N)介导心肌肥大通路的影响及 Ang 的致心肌肥厚作用。方法 :腹主动脉缩窄法建立大鼠压力超负荷模型 ,放射免疫法检测血浆、心肌 Ang 浓度 ,Western Blot检测心肌 Ca N,活化 T细胞核因子 ( NFAT3 )蛋白表达 ,发色底物法检测 Ca N比活性 ,并测定左室重量指数。结果 :缬沙坦组血浆 Ang 浓度高于对照组 ( P<0 .0 5 ) ,心肌 Ang 浓度则相反 ( P<0 .0 5 ) ,缬沙坦组 Ca N表达及活性低于对照组 ( P<0 .0 1) ,NFAT3 表达低于对照组 ( P<0 .0 5 ) ,两组各项指标与假手术组相比均差异显著。结论 :Ang 参与激活压力负荷下钙调神经磷酸酶通路 。 展开更多
关键词 缬沙坦 神经改酶 信号通路 活化T细胞核因子 血管紧张素Ⅱ
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具有PxIxIT模序的钙调磷酸酶底物或靶蛋白的研究进展
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作者 李佳娟 马彦 《中华临床医师杂志(电子版)》 CAS 2017年第1期99-103,共5页
钙调磷酸酶(CaN)广泛存在于真核生物中并且在进化上相对保守,是临床常用的免疫抑制剂环孢素A(CsA)和他克莫司(FK506)的靶蛋白,它能识别各种底物,并且控制真核细胞中的各种发育和生理功能相关的传导路径。在CaN的多种底物中有类似PxIxIT... 钙调磷酸酶(CaN)广泛存在于真核生物中并且在进化上相对保守,是临床常用的免疫抑制剂环孢素A(CsA)和他克莫司(FK506)的靶蛋白,它能识别各种底物,并且控制真核细胞中的各种发育和生理功能相关的传导路径。在CaN的多种底物中有类似PxIxIT模序存在,并且该模序内任何一个氨基酸的精细变动可以同各底物相应的生理信号传导相对应。由此可见,PxIxIT模序在真核生物生理功能的正常发挥中有着重要的作用。该文对与CaN相互作用并且具有类似PxIxIT模序的底物与靶蛋白进行了综述。 展开更多
关键词 调磷酸酶 PxIxIT模序 活化T细胞核因子 真核生物
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磷调素的研究进展
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作者 李峻岭 郑法雷 《世界医学杂志》 2003年第21期43-45,共3页
磷离子是体内许多生理过程中必不可少的元素。肠道和肾脏的调节对于保持细胞外液的磷离子浓度至关重要。长期以来,甲状旁腺激素(PTH)和l,25(0H):D3这些主要调节钙平衡的激素被认为也是调节磷平衡的重要物质。近来国外发现一组主要调... 磷离子是体内许多生理过程中必不可少的元素。肠道和肾脏的调节对于保持细胞外液的磷离子浓度至关重要。长期以来,甲状旁腺激素(PTH)和l,25(0H):D3这些主要调节钙平衡的激素被认为也是调节磷平衡的重要物质。近来国外发现一组主要调节磷平衡的蛋白,称为“磷调素(phosphatonin)^n.磷调素在一些疾病中显著升高,如肿瘤引起的骨软化(oncogenic osteomalacia,OOM)、常染色体显性遗传低磷血症性佝偻病(autosomal dominant hypophosphatemic rickets,ADHR)、X-连锁低磷血症性佝偻病(X-linked hypophosphatemic rickets,XLHR)等。这些疾病的共同特征为高尿磷、低磷血症、l,25(OH)2D3的异常和骨骼矿化障碍等,而PTH和血钙水平并无明显变化[1-2]。本文就磷调素的研究进展做一综述。 展开更多
关键词 研究进展 甲状旁腺激素 常染色体显性遗传低血症性佝偻病
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目前对磷代谢调节有何最新认识
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作者 AubreyBlumsohn 刘燕萍 郑法雷 《世界医学杂志》 2004年第13期50-53,共4页
目的:有关肾脏磷消耗的疾病,包括肿瘤性软骨病(tumor-induced osteomalacia,TIO)、X-连锁低磷血症性佝偻病(X-linked hypophosphatemic rickets,XLH)以及常染色体遗传的低磷血症(autosomal dominant hypophosphatemicrickets,ADHR... 目的:有关肾脏磷消耗的疾病,包括肿瘤性软骨病(tumor-induced osteomalacia,TIO)、X-连锁低磷血症性佝偻病(X-linked hypophosphatemic rickets,XLH)以及常染色体遗传的低磷血症(autosomal dominant hypophosphatemicrickets,ADHR),引发了人们对特异性调节磷代谢的激素——“磷调素”(phosphatonins)的研究兴趣。本综述着重讨论有哪些激素属于“磷调素”,以及它们可能的生理学特性。最近发现:可能的“磷调素”有成纤维细胞生长因子-23(fibroblast growth factor23,FGF.23)、基质胞外磷糖蛋白(matrix extracellular phosphoglycoprotein,MEPE)、stanniocalcin、Frizzled-relatedprotein4(FRP-4)。FGF-23是最主要的一种,它在导致TIO的大部分肿瘤中有表达。在大多数患有XLH、TIO的患者血清FGF-23是升高的。注射重组FGF-23导致实验动物磷酸盐尿、低磷血症以及1,25(OH)2D的抑制。目前尚有许多问题没有答案,包括PHEX(X染色体内肽酶同源性磷调基因)突变和FGF一23增加。是否这些可能的“磷调素”在健康人磷及维生素D代谢中发挥作用,以及这种作用是否通过内分泌方式也还有待研究阐明。最直接的证据是在FGF-23基因敲除小鼠观察到血磷及1,25(OH)2D升高。应该有一种在生理状态下存在的“磷调素”,以解释肾脏对不同范围的膳食磷摄入的适应性。血清FGF-23的组织来源和测定方法尚待确定。小结:毫无疑问,CA125作为免疫治疗,尤其是作为细胞治疗新发展的重要靶抗原将再次辉煌。而且,对其他卵巢肿瘤新抗原的认识也将进一步加强T细胞免疫的研究。目前的研究趋势是:从强调设计产生抗体反应的疫苗转向能够激发更强的免疫反应,包括卵巢肿瘤抗原特异的辅助T淋巴细胞和细胞毒T淋巴细胞反应,如树突状细胞疫苗的研究。 展开更多
关键词 代谢 节机制 成纤维细胞生长因子-23 病理学 生理学
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肿瘤性骨软化症1例并文献复习 被引量:6
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作者 易维 刘建 +1 位作者 夏秦 乔礼芬 《内科急危重症杂志》 2014年第1期39-41,共3页
目的:通过典型病例了解肿瘤性骨软化症(TIO)的临床特点及诊疗方法,提高对TIO的认识水平。方法:对1例TIO患者的临床资料进行整理和分析,并复习相关文献。结果:此例患者表现为全身骨关节痛、活动障碍、血磷下降、肾小管磷重吸收率下降,经6... 目的:通过典型病例了解肿瘤性骨软化症(TIO)的临床特点及诊疗方法,提高对TIO的认识水平。方法:对1例TIO患者的临床资料进行整理和分析,并复习相关文献。结果:此例患者表现为全身骨关节痛、活动障碍、血磷下降、肾小管磷重吸收率下降,经68Ga-TATE PET/CT及18-FDG PET/CT检查发现右肛周肿瘤,手术后第2天血磷恢复正常,病理报告为磷酸盐尿性间叶组织肿瘤。结论:对临床表现为全身骨痛、低血磷、肾小管磷重吸收率下降的患者,需高度怀疑TIO,建议积极行影像学检查寻找肿瘤病灶。 展开更多
关键词 肿瘤性骨软化症 血症 调磷因子
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钙激动剂介导血管平滑肌细胞增殖的信号转导途径 被引量:10
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作者 杨永健 朱峻 +2 位作者 周兴文 张鑫 赵龙生 《中国动脉硬化杂志》 CAS CSCD 2001年第1期37-39,共3页
为探讨钙调神经磷酸酶依赖的信号通路在雷尼丁剌激的大鼠血管平滑肌细胞增殖中的作用 ,以培养的大鼠血管平滑肌细胞为模型 ,用雷尼丁剌激其内贮Ca2 + 释放入胞浆 ,环孢素A阻断钙调神经磷酸酶信号通路 ,维拉帕米阻断钙通道 ,检测血管平... 为探讨钙调神经磷酸酶依赖的信号通路在雷尼丁剌激的大鼠血管平滑肌细胞增殖中的作用 ,以培养的大鼠血管平滑肌细胞为模型 ,用雷尼丁剌激其内贮Ca2 + 释放入胞浆 ,环孢素A阻断钙调神经磷酸酶信号通路 ,维拉帕米阻断钙通道 ,检测血管平滑肌细胞钙调神经磷酸酶、丝裂素活化蛋白激酶和蛋白激酶C活性 ,用 3 H -亮氨酸及3 H -胸腺嘧啶掺入量作为反应细胞增殖的指标。结果显示 ,雷尼丁剌激组蛋白核酸合成速率明显增高 ,与对照组相比差异显著 (P <0 .0 1) ;环孢素A及维拉帕米能明显抑制雷尼丁介导的平滑肌细胞蛋白核酸合成速率增高 ,与雷尼丁剌激组相比差异显著 (P <0 .0 1)。同时发现雷尼丁剌激组钙调神经磷酸酶、蛋白激酶C活性与对照组平滑肌细胞相比差异显著 (P <0 .0 5或 0 .0 1)。环孢素A和维拉帕米抑制雷尼丁介导的平滑肌细胞钙调神经磷酸酶活性增高 ,维拉帕米抑制雷尼丁介导的平滑肌细胞蛋白激酶C活性的增高。提示钙调神经磷酸酶信号通路在雷尼丁剌激的平滑肌细胞增殖中起重要作用 ,但钙调神经磷酸酶信号通路不是雷尼丁介导平滑肌细胞增殖的唯一信号通路 ,以丝裂素活化蛋白激酶为核心的信号通路亦参与了雷尼丁剌激的平滑肌细胞增殖。 展开更多
关键词 神经 细胞增殖 信号通路 血管平滑肌
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成纤维细胞生长因子23与慢性肾脏病患者心血管疾病的关系 被引量:2
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作者 徐超 张蕾 《新疆医学》 2017年第1期81-83,共3页
慢性肾脏病(chronic kidney disease,CKD)作为当今世界所面临的严峻的公共卫生挑战[1],同样也是我们所需解决的棘手的问题。CKD存在病因复杂,病程长,预后影响因素多,个体差异大等特点,且受社会及个体经济条件影响大。CKD患者的首位死... 慢性肾脏病(chronic kidney disease,CKD)作为当今世界所面临的严峻的公共卫生挑战[1],同样也是我们所需解决的棘手的问题。CKD存在病因复杂,病程长,预后影响因素多,个体差异大等特点,且受社会及个体经济条件影响大。CKD患者的首位死亡原因是心血管疾病,并使现今社会背负了巨大的医疗压力[2]。 展开更多
关键词 心血管疾病 调磷因子 肾脏病患者 个体差异 公共卫生 死亡原因 血管钙化 血症 收缩性心衰
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Roles of TRESK,a novel two-pore domain K+channel,in pain pathway and general anesthesia 被引量:1
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作者 黄东越 于布为 范秋维 《Neuroscience Bulletin》 SCIE CAS CSCD 2008年第3期166-172,共7页
TRESK is the most recently reported two-pore domain K^+ channel, and different from other two-pore domain channels in gene, molecular structure, electrophysiological and pharmacological properties. Although the curre... TRESK is the most recently reported two-pore domain K^+ channel, and different from other two-pore domain channels in gene, molecular structure, electrophysiological and pharmacological properties. Although the current knowledge of this potassium channel is inadequate, researches have demonstrated that TRESK is remarkablely linked to acute and chronic pain by activation of calcineurin. The fact that TRESK is sensitive to volatile anesthetics and localization in central nerve system implies that TRESK may play a very important role in the mechanism mediating general anesthesia. The further research of TRESK may contribute to explore the underlying mechanism of some pathological conditions and yield novel treatments for some diseases. 展开更多
关键词 TRESK two-pore domain K^+ channels CALCINEURIN dorsal root ganglion
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The New Plating Process on Plastics
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作者 Makoto Imamura Jun Okada Kazuya Satoh 《材料热处理学报》 EI CAS CSCD 北大核心 2004年第05B期1119-1121,共3页
This process does not use general electroless plating solution containing formaldehyde(carcinogen) and phosphate(the object of waste water regulation). Furthermore, we succeeded in developing a new electroless copper ... This process does not use general electroless plating solution containing formaldehyde(carcinogen) and phosphate(the object of waste water regulation). Furthermore, we succeeded in developing a new electroless copper plating solution which is usable semi-permanently by using the special reducing agent. The electroless deposition forms a very thin conductive film on substrate. Therefore, it can prevent some appearance defects (e.g. pits, pin-holes, laminations and inclusions) that occur in the conventional ones. In addition, it is possible to eliminate electroplating of copper from the process by applying the exclusive nickel solution in electroplating. Therefore, the obtained metal parts can be recycled as high quality stainless steel. Given this copper electroplating-free process, 25 u m-thick nickel deposition at the stage of electroplating, at least, enables the metal layers to show properties as versatile as those of the conventional(its total thickness is 25 n m in the comparative conventional process). 展开更多
关键词 电镀 再循环 调磷 合成树脂
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新型高效水处理剂的研制 被引量:2
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作者 路嫔 蔡清海 梁海燕 《化学工程师》 CAS 2003年第1期1-3,共3页
本文简单介绍了2-丙烯酰胺-2-甲基丙磺酸(AMPS)的性能及应用,工业循环冷却水处理中阻垢剂的发展历史与最新进展。并首次以马来酸酐(MA)、AMPS、NaH2PO2为单体,水为溶剂、过硫酸氨为引发剂合成低磷含量的膦羧酸共调聚物,探讨了单体配比为... 本文简单介绍了2-丙烯酰胺-2-甲基丙磺酸(AMPS)的性能及应用,工业循环冷却水处理中阻垢剂的发展历史与最新进展。并首次以马来酸酐(MA)、AMPS、NaH2PO2为单体,水为溶剂、过硫酸氨为引发剂合成低磷含量的膦羧酸共调聚物,探讨了单体配比为n(MA):n(AMPS)=2:1;m(NaH2PO2):m(MA+AMPS)=10:100时反应温度,反应时间对合成产品的影响。 展开更多
关键词 新型 高效水处理剂 研制 阻垢剂 含量膦羧酸共聚物 水处理
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Casein kinase 2 interacts with and phosphorylates ataxin-3 被引量:3
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作者 陶瑞松 费尔康 +2 位作者 应征 王洪枫 王光辉 《Neuroscience Bulletin》 SCIE CAS CSCD 2008年第5期271-277,共7页
Objective Machado-Joseph disease (MJD)/Spinocerebellar ataxia type 3 (SCA3) is an autosomal dominant neurodegenerative disorder caused by an expansion of polyglutamine tract near the C-terminus of the MJD1 gene pr... Objective Machado-Joseph disease (MJD)/Spinocerebellar ataxia type 3 (SCA3) is an autosomal dominant neurodegenerative disorder caused by an expansion of polyglutamine tract near the C-terminus of the MJD1 gene product, ataxin-3. The precise mechanism of the MJD/SCA3 pathogenesis remains unclear. A growing body of evidence demonstrates that phosphorylation plays an important role in the pathogenesis of many neurodegenerative diseases. However, few kinases are known to phosphorylate ataxin-3. The present study is to explore whether ataxin-3 is a substrate of casein kinase 2 (CK2). Methods The interaction between ataxin-3 and CK2 was identified by glutathione S-transferase (GST) pull-down assay and co-immunoprecipition assay. The phosphorylation of ataxin-3 by CK2 was measured by in vitro phosphorylation assays. Results (1) Both wild type and expanded ataxin-3 interacted with CK2α and CK2β in vitro. (2) In 293 cells, both wild type and expanded ataxin-3 interacted with CK2β, but not CK2α. (3) CK2 phosphorylated wild type and expanded ataxin-3. Conclusion Ataxin-3 is a substrate of protein kinase CK2. 展开更多
关键词 Machado-Joseph disease/spinocerebellar ataxia type 3 ATAXIN-3 casein kinase 2 PHOSPHORYLATION
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CIPK9: a calcium sensor-interacting protein kinase required for low-potassium tolerance in Arabidopsis 被引量:23
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作者 Girdhar K Pandey Yong Hwa Cheongx +3 位作者 Beom-Gi Kim John J Grant Legong Li Sheng Luan 《Cell Research》 SCIE CAS CSCD 2007年第5期411-421,共11页
Potassium is one of the major macro-nutrients essential for a number of cellular processes in plants. Low potassium level in the soil represents a limiting factor for crop production. Recent studies have identified po... Potassium is one of the major macro-nutrients essential for a number of cellular processes in plants. Low potassium level in the soil represents a limiting factor for crop production. Recent studies have identified potassium transporters that are involved in potassium acquisition, and some of them are critical for potassium nutrition under low potassium conditions. However, little is understood on the molecular components involved in low potassium signaling and responses. We report here the identification ofa calcineurin B-like protein-interacting protein kinase (CIPK9) as a critical regulator of low potassium response in ,Arabidopsis. The CIPK9 gene was responsive to abiotic stress conditions, and its transcript was inducible in both roots and shoots by potassium deprivation. Disruption of CIPK9 function rendered the mutant plants hypersensitive to low potassium media. Further analysis indicated that K^+ uptake and content were not affected in the mutant plants, implying CIPK9 in the regulation of potassium utilization or sensing processes. 展开更多
关键词 CALCIUM calcineurin-B like protein protein kinase potassium nutrition signal transduction
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Effects of Regulation of C/N Ratio Wheat Straw Application on Nitrogen,Phosphorus and Potassium Uptake in Tobacco 被引量:13
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作者 段宗颜 王瑞宝 +3 位作者 鲁耀 殷寿安 胡万里 陈拾华 《Agricultural Science & Technology》 CAS 2010年第5期77-81,共5页
[Objective]The aim was to study the effects of regulation of C/N ratio wheat straw application on tobacco nitrogen,phosphorus and potassium uptake. [Method]Effects of regulation C/N ratio wheat straw application on th... [Objective]The aim was to study the effects of regulation of C/N ratio wheat straw application on tobacco nitrogen,phosphorus and potassium uptake. [Method]Effects of regulation C/N ratio wheat straw application on the flue-cured tobacco yield,output value,nitrogen,phosphorus and potassium content and cumulative uptake of the upper,middle and bottom leaf were studied by using the field plot experiments at Banqiao town,Qujing city,Yunnan Province during the 2008-2009 summer growing seasons. [Result]The results showed that the application of wheat straw alone or after C/N regulation,could significantly increase tobacco production,potassium content,the potassium and nitrogen accumulation amount of leaf,and was more conducive to the potassium uptake of tobacco leaf with wheat straw application after C/N regulation. Compared with non-straw application,the yield of tobacco increased by 6.59%,3.58%,5.98%,8.80% with application of wheat straw alone,wheat straw and vetch,wheat straw and oilseed cake,wheat straw and urea nitrogen,the potassium content in tobacco leaf increased by 3.85%,7.76%,8.82%,11.21%,respectively,the total potassium cumulative amount of leaf increased by 10.71%,11.62%,15.32% ,21.01% and the total nitrogen cumulative amount increased by 9.76%,1.22%,8.14%,14.00%. However,the differences of tobacco leaf nitrogen content among the different treatments were not significant,the phosphorus uptake of tobacco leaf decreased. [Conclusion]application of high C/N ratio wheat straw in flue-cured tobacco production,which should be concerned not only to adjust C/N ratio by adding nitrogen,but also considering additional phosphorus application. 展开更多
关键词 Wheat straw C/N ratio regulation Flue-cured tobacco N P K uptake
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Physiological levels of ATP negatively regulate proteasome function 被引量:4
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作者 Hongbiao Huang Xiaoyan Zhang +16 位作者 Shujue Li Ningning Liu Wen Elan Emily McDowell Ping Zhou Canguo Zhao Haiping Guo Change Zhang Changshan Yang Guangmei Wen Xiaoxian Dong Li Lu Ningfang Ma Weihua Dong Q Ping Dou Xuejun Wang Jinbao Liu 《Cell Research》 SCIE CAS CSCD 2010年第12期1372-1385,共14页
Intracellular protein degradation by the ubiquitin-proteasome system is ATP dependent, and the optimal ATP concentration to activate proteasome function in vitro is -100 μM. IntraceUular ATP levels are generally in t... Intracellular protein degradation by the ubiquitin-proteasome system is ATP dependent, and the optimal ATP concentration to activate proteasome function in vitro is -100 μM. IntraceUular ATP levels are generally in the low millimolar range, but ATP at a level within this range was shown to inhibit proteasome peptidase activities in vitro. Here, we report new evidence that supports a hypothesis that intracellular ATP at the physiological levels bidirectionally regulates 26S proteasome proteolytic function in the cell. First, we confirmed that ATP exerted bidirectional regulation on the 26S proteasome in vitro, with the optimal ATP concentration (between 50 and 100μM) stimulating proteasome chymotrypsin-like activities. Second, we found that manipulating intracellular ATP levels also led to bidirectional changes in the levels of proteasome-specific protein substrates in cultured cells. Finally, measures to increase intracellular ATP enhanced, while decreasing intraceHular ATP attenuated the ability of proteasome inhibition to induce cell death. These data strongly suggest that endogenous ATP within the physiological concentration range can exert a negative impact on proteasome activities, allowing the cell to rapidly upregulate proteasome activity on ATP reduction under stress conditions. 展开更多
关键词 ATP PROTEASOME REGULATION APOPTOSIS
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Variation of alkaline characteristics in bauxite residue under phosphogypsum amendment 被引量:25
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作者 LI Yi-wei LUO Xing-hua +2 位作者 LI Chu-xuan JIANG Jun XUE Sheng-guo 《Journal of Central South University》 SCIE EI CAS CSCD 2019年第2期361-372,共12页
Aiming at alkaline problem of bauxite residue,this work focused variation of alkaline characteristics in bauxite residue through phosphogypsum treatment.The results demonstrated that the pH of bauxite residue reduced ... Aiming at alkaline problem of bauxite residue,this work focused variation of alkaline characteristics in bauxite residue through phosphogypsum treatment.The results demonstrated that the pH of bauxite residue reduced from initial 10.83 to 8.70 when 1.50 wt%phosphogypsum was added for 91 d.The removal rates of free alkali and exchangeable sodium were 97.94%and 75.87%,respectively.Meanwhile,significant positive correlations(P<0.05)existed between pH and free alkali,exchangeable sodium.The effect of free alkali composition was CO3^2–>OH^–>AlO2^–>HCO3^–.In addition,alkaline phase decreased from 52.81%to 48.58%and gypsum stably presented in bauxite residue which continuously provided Ca^2+to inhibit dissolution of combined alkali.Furthermore,phosphogypsum promoted formation of macroaggregate structure,increased Ca^2+,decreased Na+and Al^3+on the surface of bauxite residue significantly,ultimately promoting soil formation in bauxite residue. 展开更多
关键词 bauxite residue alkaline regulation free alkali phosphogypsum amendment soil formation in bauxite residue
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Metabolic Control and Growth Regulation in the Regenerating Human Liver Measured in Vivo by 31—phosophorus Magnetic Resonance Spectroscopy
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作者 文达伦 刘允怡 《The Chinese-German Journal of Clinical Oncology》 CAS 2002年第3期152-155,共4页
Objectives Partial hepatectomy induces a rapid transformation within the remnant liver, prompting a wave of hepatocyte mitosis which abates when the lost cell mass has been recovered. The mechanism of co-ordinated con... Objectives Partial hepatectomy induces a rapid transformation within the remnant liver, prompting a wave of hepatocyte mitosis which abates when the lost cell mass has been recovered. The mechanism of co-ordinated control of metabolism and maintenance of function during this period of dynamic change is incompletely understood. Furthermore, the biochemical basis of growth regulation in the regenerating liver has not been well defined. We have studied human liver regeneration using in vivo 31-phosphorus magnetic resonance spectroscopy (31 P MRS). This non-invasive technique allows assay of high-energy phosphate compounds and also of phospholipid metabolites thought to be involved in cellular renewal processes.Methods Five patients undergoing liver resection were studied. Hepatic metabolism was evaluated using 31P MRS before surgery and on postoperative days 2,4,6 and 14. Estimation of liver volume by magnetic resonance imaging and blood sampling for biochemistry were performed at the same time points.Results We found that the regenerative response following loss of liver parenchyma produced a reversible decline in energy state which necessitated compensatory adjustments in liver synthetic and excretory (unctions. Volume regain was associated with alterations in phospholipid metabolism, which normalized when the hepatic growth spurt was completed.Conclusion These observations indicate that modulation of hepatocyte energy economy is necessary for the integrated recovery of liver cell mass and function. We propose that deficient hepatic energy production may explain the mechanism of liver failure after hepatectomy, and suggest that in vivo measurement of liver metabolism may provide a rational basis for the development and evaluation of hepatic support strategies. 展开更多
关键词 HEPATOCYTE MITOSIS METABOLISM ENERGY phosphollpid
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Characterization of DYRK2(dual-specificity tyrosine-phosphorylation-regulated kinase 2) from Zebrafish(Dario rerio)
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作者 孙威 谭训刚 +2 位作者 张培军 张玉青 徐永立 《Chinese Journal of Oceanology and Limnology》 SCIE CAS CSCD 2010年第4期720-724,共5页
Proteins of the DYRK(dual-specificity tyrosine-phosphorylation-regulated kinase) family are characterized by the presence of a conserved kinase domain and N-terminal DH box.DYRK2 is involved in regulating key developm... Proteins of the DYRK(dual-specificity tyrosine-phosphorylation-regulated kinase) family are characterized by the presence of a conserved kinase domain and N-terminal DH box.DYRK2 is involved in regulating key developmental and cellular processes,such as neurogenesis,cell proliferation,cytokinesis,and cellular differentiation.Herein,we report that the ortholog of DYRK2 found in zebrafish shares about 70% identity with that of human,mouse,and chick.RT-PCR showed that DYRK2 is expressed maternally and zygotically.In-situ hybridization results show that DYRK2 is expressed in somite cells that will develop into muscles.Our results provide preliminary evidence for investigating the in-vivo function of DYRK2 in zebrafish muscle development. 展开更多
关键词 ZEBRAFISH DYRK2 in-situ hybridization muscle development
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Calcineurin signalling mechanisms in myocardial hypertrophy
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作者 Jian-Chun Wang Yong Zhao Jian-Hua Shao 《Journal of Geriatric Cardiology》 SCIE CAS CSCD 2010年第3期189-192,共4页
Calcineurin dephosphorylates multiple serine residues near the N terminus of NFAT proteins enabling them to translocate from cytoplasm to nucleus, where they activate a subset of hypertrophic response genes. Transgeni... Calcineurin dephosphorylates multiple serine residues near the N terminus of NFAT proteins enabling them to translocate from cytoplasm to nucleus, where they activate a subset of hypertrophic response genes. Transgenic mice over-expressing a constitu- tively active form of calcineurin or NFAT3, developed obviously hypertrophy and heart failure or sudden death proving its pathogenic role. Here we used literatures on MEDLINE (2000-2011), systematically reviewed the new development of calcineurin signaling pathway in myocardial hypertrophy 展开更多
关键词 CALCINEURIN signal transduction HYPERTROPHY
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