[ Objective] The purpose was to discuss drought resistance mechanism of Chenopodium album L. and supply theoretical basis and practical guidance for artificial cultivation and popularization of C. album. [ Method] C. ...[ Objective] The purpose was to discuss drought resistance mechanism of Chenopodium album L. and supply theoretical basis and practical guidance for artificial cultivation and popularization of C. album. [ Method] C. album seedlings grown to 6th leaf stage were conducted osmotic stress treatment with PEG6000 osmotic whose concentration was set up as 0, 5%, 10% and 20% and the various physiological indices of the 3rd -5th function leaves in upper plant were determined after being treated for 0, 1,3, 5, 7 and 9 d. [ Result] Under osmotic stress with 5% PGE, the relative water content (RWC) of C. album reduced less. Under osmotic stress with 10%, the RWC in seedling leaves of C. album decreased to 62% on the fifth day and the leaves began to wither. Under osmotic stress with 20%, the RWC in seedling leaves of C. album decreased to 61.9% on the third day and the leaves appeared withering, and the RWC decreased to 48.6% on the 7th day and the leaves were dry and yellow. Proline contents in seedling leaves of C. album under osmotic stress with 5%, 10% and 20% PEG were 7.64, 10.9 and 29.4 times of CK on the 7th day. [ Conclusion] C. album hed some adaptability to moderate osmotic stress, but the PEG osmotic stress with high concentration and long time might lead to severe damage on C. album.展开更多
Based on immune network regulatory mechanism, a new adaptive immune evolutionary algorithm (AIEA) is proposed to improve the performance of genetic algorithms (GA) in this paper. AIEA adopts novel selection operation ...Based on immune network regulatory mechanism, a new adaptive immune evolutionary algorithm (AIEA) is proposed to improve the performance of genetic algorithms (GA) in this paper. AIEA adopts novel selection operation according to the stimulation level of each antibody. A memory base for good antibodies is devised simultaneously to raise the convergent rapidity of the algorithm and adaptive adjusting strategy of antibody population is used for preventing the loss of the population adversity. The experiments show AIEA has better convergence performance than standard genetic algorithm and is capable of maintaining the adversity of the population and solving function optimization problems in an efficient and reliable way.展开更多
Iodothyronine deiodinase catalyzes the initiation and termination of thyroid hormones(THs) effects, and plays a central role in the regulation of thyroid hormone level in vertebrates. In non-chordate invertebrates, on...Iodothyronine deiodinase catalyzes the initiation and termination of thyroid hormones(THs) effects, and plays a central role in the regulation of thyroid hormone level in vertebrates. In non-chordate invertebrates, only one deiodinase has been identified in the scallop C hlamys farreri. Here, two deiodinases were cloned in the Pacific oyster C rassostrea gigas( Cg Dx and C g Dy). The characteristic in-frame TGA codons and selenocysteine insertion sequence elements in the oyster deiodinase c DNAs supported the activity of them. Furthermore, seven orthologs of deiodinases were found by a tblastn search in the mollusk Lottia gigantea and the annelid C apitella teleta. A phylogenetic analysis revealed that the deiodinase gene originated from an common ancestor and a clade-specific gene duplication occurred independently during the differentiation of the mollusk, annelid, and vertebrate lineages. The distinct spatiotemporal expression patterns implied functional divergence of the two deiodinases. The expression of C g Dx and Cg Dy was influenced by L-thyroxine T4, and putative thyroid hormone responsive elements were found in their promoters, which suggested that the oyster deiodinases were feedback regulated by TH. Epinephrine stimulated the expression level of C g Dx and Cg Dy, suggesting an interaction effect between different hormones. This study provides the first evidence for the existence of a conserved TH feedback regulation mechanism in mollusks, providing insights into TH evolution.展开更多
Proline-rich tyrosine kinase 2 (Pyk2) is a nonreceptor protein tyrosine kinase,which is also known as Ca2 +-dependent tyrosine kinase or related adhesion focal tyrosine kinase.Pyk2 activation exerts a critical regulat...Proline-rich tyrosine kinase 2 (Pyk2) is a nonreceptor protein tyrosine kinase,which is also known as Ca2 +-dependent tyrosine kinase or related adhesion focal tyrosine kinase.Pyk2 activation exerts a critical regulatory mechanism for various physiological processes including cytoskeleton function,regulation of cell growth and death,modulation of ion channels and multiple signaling events.However,mechanisms underlying the functional diversity of Pyk2 are not clear.A Pyk2 isoform that encodes only part of the C-terminal domain of Pyk2,named as PRNK (Pyk2-related non-kinase),acts as a dominant-negative inhibitor of Pyk2-dependent signaling by displacing Pyk2 from focal adhesions.Research on functional PRNK probably provides new potential inhibitory tool targeting Pyk2 and makes it possible to explore more of Pyk2 pathological mechanism.PRNK is a promising candidate targeting Pyk2 modulation.This review focuses on the functional investigation of Pyk2 and its structure and localization,including recent research with inhibitory strategies targeting Pyk2 by the method of PRNK.展开更多
To increase accuracy of navigation parameters,a perspective measuring complex with intellectual components is developed.Conception of synthesis optimal structure of the measuring complex is realized basing on a select...To increase accuracy of navigation parameters,a perspective measuring complex with intellectual components is developed.Conception of synthesis optimal structure of the measuring complex is realized basing on a selective method using principles of the functional systems.Selection of measured information is finished by original numeric criterion of observation level of state vector components.Prediction is realized by algorithm of self-organization that makes synthesis of the optimal complication.Therefore mechanism of self-regulation is realized and accuracy of the selective navigation complex is increased.展开更多
With the analysis on regulating system in 200 MW steam turbine, the necessity of appending the fast-opening function to the original system is set forth and a new type of fast-opening mechanism is devised. The mathema...With the analysis on regulating system in 200 MW steam turbine, the necessity of appending the fast-opening function to the original system is set forth and a new type of fast-opening mechanism is devised. The mathematical model of system is built up. With the use of AMESIM software, the displacement curve of the piston, the force curve of the cartridge valve spool, the pressure curve and the flux curve in the regulation process are obtained based on simulation. The performances of three fast-opening systems composed of cartridge valves with different diameters are compared. Based on the analysis on factors that affect the execution time of fast-opening, the dead zone of the fast-opening system is put forward. To overcome the defect, dif- ferent operation modes are adopted for different zones. The result shows that with the increase of the valve diameter, the regulating time in the dead zone significantly exceeds the fast-opening time in the whole journey. Accordingly, the optimization operation tactic in the dead zone and the qualification conditions are brought forward. The fast-opening system composed of 32 mm cartridge valves is taken as an example with use of the tactic. The simulation result shows that the maximum regulating time is shortened by 509 ms.展开更多
Camera calibration is a critical process in photogrammetry and a necessary step to acquire 3D information from a 2D image. In this paper, a flexible approach for CCD camera calibration using 2D direct linear transform...Camera calibration is a critical process in photogrammetry and a necessary step to acquire 3D information from a 2D image. In this paper, a flexible approach for CCD camera calibration using 2D direct linear transformation (DLT) and bundle adjustment is proposed. The proposed approach assumes that the camera interior orientation elements are known, and addresses a new closed form solution in planar object space based on homogenous coordinate representation and matrix factorization. Homogeneous coordinate representation offers a direct matrix correspondence between the parameters of the 2D DLT and the collinearity equation. The matrix factorization starts by recovering the elements of the rotation matrix and then solving for the camera position with the collinearity equation. Camera calibration with high precision is addressed by bundle adjustment using the initial values of the camera orientation elements. The results show that the calibration precision of principal point and focal length is about 0.2 and 0.3 pixels respectivelv, which can meet the requirements of close-range photogrammetry with high accuracy.展开更多
The issue of employer branding has always had an intrinsic interdisciplinary content, since it builds a bridge for cross-fertilization between different disciplines (strategic marketing, strategic management, and hum...The issue of employer branding has always had an intrinsic interdisciplinary content, since it builds a bridge for cross-fertilization between different disciplines (strategic marketing, strategic management, and human resources management). This paper presents employer branding as an approach based on effective strategic organizational resources and a precise employer branding management process. In this paper, the target is to define a possible frame in order to interpret employer branding techniques as control and regulation mechanisms. Control in organizations has long been a topic of interest for researchers and practitioners, alike who generally recognize that control mechanisms are needed to ensure that organizations may achieve their goals. It has been carried out a field work on the Italian Aeronautical Meta district, that generates annual revenues equal to EUR 8.7 billion and employees 36,300 people, of whom approximately 10% are employed in the space sector. Through this field work, the intention aim is to understand if and how employer branding may create, enforce, and set up internal and shared meaning and values. In particular, stemming from the empirical research's evidences, this paper tries to conceptualize employer branding as a factor to persuade and influence the way that organizational actors enact in a socially constructed context. In this effort, the aim is to understand how managers may affect meanings, values goals, and opinions through an effective action on employer branding that can play a crucial role in affecting sense-making processes, meanings, interpretations of the symbolic environment. The paper therefore opens up to new horizons, since it embraces a new application for employer branding, considering it as a modern control system and proposing an innovative approach in managerial control, founded on organizational identity as a key concept in an organizational citizenship's perspective展开更多
MicroRNAs (miRNAs) are small, non-coding single-stranded RNAs that can modulate target gene expression at post- transcriptional level and participate in cell proliferation, differentiation, and apoptosis. T cells ha...MicroRNAs (miRNAs) are small, non-coding single-stranded RNAs that can modulate target gene expression at post- transcriptional level and participate in cell proliferation, differentiation, and apoptosis. T cells have important functions in acquired immune response; miRNAs regulate this immune response by targeting the mRNAs of genes involved in T cell developmentp proliferationj differentiationp and function. For instancep miR-181 family members function in progression by targeting Bcl2 and CD69, among others. MiR-17 to miR-92 clusters function by binding to CREB 1, PTEN, and Bim. Considering that the suppression ofT cell-mediated immune responses against tumor cells is involved in cancer progression, we should investigate the mechanism by which miRNA regulates T cells to develop new approaches for cancer treatment.展开更多
AIM:To investigate the pathway(s)mediating rat antral circular smooth muscle contractile responses to the cholinomimetic agent,bethanechol and the subtypes of muscarinic receptors mediating the cholinergic contraction...AIM:To investigate the pathway(s)mediating rat antral circular smooth muscle contractile responses to the cholinomimetic agent,bethanechol and the subtypes of muscarinic receptors mediating the cholinergic contraction. METHODS:Circular smooth muscle strips from the antrum of Sprague-Dawley rats were mounted in muscle baths in Krebs buffer.Isometric tension was recorded.Cumulative concentration-response curves were obtained for(+)-cis- dioxolane(cD),a nonspecific muscarinic agonist,at 10^(-8)- 10^(-4)mol/L,in the presence of tetrodotoxin(TTX,10^(-7)mol/L). Results were normalized to cross sectional area.A repeat concentration-response curve was obtained after incubation of the muscle for 90 min with antagonists for M1(pirenzepine), M2(methoctramine)and M3(darifenadn)muscarinic receptor subtypes.The sensitivity to PTX was tested by the ip injection of 100 mg/kg of PTX 5 d before the experiment.The antral circular smooth muscles were removed from PTX-treated and non-treated rats as strips and dispersed smooth muscle cells to identify whether PTX-linked pathway mediated the contractility to bethanechol. RESULTS:A dose-dependent contractile response observed with bethanechol,was not affected by TTx.The pretreatment of rats with pertussis toxin decreased the contraction induced by bethanechol.Lack of calcium as well as the presence of the L-type calcium channel blocker,nifedipine,also inhibited the cholinergic contraction,with a reduction in response from 2.5±0.4 g/mm^2 to 1.2±0.4 g/mm^2(P<0.05).The dose- response curves were shifted to the right by muscarinic antagonists in the following order of affinity:darifenacin (M_3)>methocramine(M_2)>pirenzepine(M_1). CONCLUSION:The muscarinic receptors-dependent contraction of rat antral circular smooth muscles was linked to the signal transduction pathway(s)involving pertussis-toxin sensitive GTP-binding proteins and to extracellular calcium via L-type voltage gated calcium channels.The presence of the residual contractile response after the treatment with nifedipine,suggests that an additional pathway could mediate the cholinergic contraction.The involvement of more than one muscarinic receptor(functionally predominant type 3 over type 2)also suggests more than one pathway mediating the cholinergic contraction in rat antrum.展开更多
基金Supported by the Natural Science Foundation of Education Department of Jiangsu Province(02KJD18007)the Key Laboratory Program of Bio-re-sources of Jiangsu Province(KJS03042)the Key Program of Natural Science Foundation of Xuzhou Normal University(06XLA11)~~
文摘[ Objective] The purpose was to discuss drought resistance mechanism of Chenopodium album L. and supply theoretical basis and practical guidance for artificial cultivation and popularization of C. album. [ Method] C. album seedlings grown to 6th leaf stage were conducted osmotic stress treatment with PEG6000 osmotic whose concentration was set up as 0, 5%, 10% and 20% and the various physiological indices of the 3rd -5th function leaves in upper plant were determined after being treated for 0, 1,3, 5, 7 and 9 d. [ Result] Under osmotic stress with 5% PGE, the relative water content (RWC) of C. album reduced less. Under osmotic stress with 10%, the RWC in seedling leaves of C. album decreased to 62% on the fifth day and the leaves began to wither. Under osmotic stress with 20%, the RWC in seedling leaves of C. album decreased to 61.9% on the third day and the leaves appeared withering, and the RWC decreased to 48.6% on the 7th day and the leaves were dry and yellow. Proline contents in seedling leaves of C. album under osmotic stress with 5%, 10% and 20% PEG were 7.64, 10.9 and 29.4 times of CK on the 7th day. [ Conclusion] C. album hed some adaptability to moderate osmotic stress, but the PEG osmotic stress with high concentration and long time might lead to severe damage on C. album.
基金National Science Funds for Distinguished Young Scholars ( No60625302)Major state Basic Research Program ofChina (973Program) (No2002CB312200) +1 种基金the 863 Hi-Tech Research and Development Programof China (No20060104Z1081)Science and Research Program of Shanghai Educational Committee (No06DZ030)
文摘Based on immune network regulatory mechanism, a new adaptive immune evolutionary algorithm (AIEA) is proposed to improve the performance of genetic algorithms (GA) in this paper. AIEA adopts novel selection operation according to the stimulation level of each antibody. A memory base for good antibodies is devised simultaneously to raise the convergent rapidity of the algorithm and adaptive adjusting strategy of antibody population is used for preventing the loss of the population adversity. The experiments show AIEA has better convergence performance than standard genetic algorithm and is capable of maintaining the adversity of the population and solving function optimization problems in an efficient and reliable way.
基金Supported by the National Natural Science Foundation of China(Nos.31372515,31402285)the National Basic Research Program of China(973 Program)(No.2010CB126401)the National High Technology Research and Development Program(863 Program)(No.2012AA10A405)
文摘Iodothyronine deiodinase catalyzes the initiation and termination of thyroid hormones(THs) effects, and plays a central role in the regulation of thyroid hormone level in vertebrates. In non-chordate invertebrates, only one deiodinase has been identified in the scallop C hlamys farreri. Here, two deiodinases were cloned in the Pacific oyster C rassostrea gigas( Cg Dx and C g Dy). The characteristic in-frame TGA codons and selenocysteine insertion sequence elements in the oyster deiodinase c DNAs supported the activity of them. Furthermore, seven orthologs of deiodinases were found by a tblastn search in the mollusk Lottia gigantea and the annelid C apitella teleta. A phylogenetic analysis revealed that the deiodinase gene originated from an common ancestor and a clade-specific gene duplication occurred independently during the differentiation of the mollusk, annelid, and vertebrate lineages. The distinct spatiotemporal expression patterns implied functional divergence of the two deiodinases. The expression of C g Dx and Cg Dy was influenced by L-thyroxine T4, and putative thyroid hormone responsive elements were found in their promoters, which suggested that the oyster deiodinases were feedback regulated by TH. Epinephrine stimulated the expression level of C g Dx and Cg Dy, suggesting an interaction effect between different hormones. This study provides the first evidence for the existence of a conserved TH feedback regulation mechanism in mollusks, providing insights into TH evolution.
基金Supported by the National Natural Science Foundation of China(30700822)
文摘Proline-rich tyrosine kinase 2 (Pyk2) is a nonreceptor protein tyrosine kinase,which is also known as Ca2 +-dependent tyrosine kinase or related adhesion focal tyrosine kinase.Pyk2 activation exerts a critical regulatory mechanism for various physiological processes including cytoskeleton function,regulation of cell growth and death,modulation of ion channels and multiple signaling events.However,mechanisms underlying the functional diversity of Pyk2 are not clear.A Pyk2 isoform that encodes only part of the C-terminal domain of Pyk2,named as PRNK (Pyk2-related non-kinase),acts as a dominant-negative inhibitor of Pyk2-dependent signaling by displacing Pyk2 from focal adhesions.Research on functional PRNK probably provides new potential inhibitory tool targeting Pyk2 and makes it possible to explore more of Pyk2 pathological mechanism.PRNK is a promising candidate targeting Pyk2 modulation.This review focuses on the functional investigation of Pyk2 and its structure and localization,including recent research with inhibitory strategies targeting Pyk2 by the method of PRNK.
文摘To increase accuracy of navigation parameters,a perspective measuring complex with intellectual components is developed.Conception of synthesis optimal structure of the measuring complex is realized basing on a selective method using principles of the functional systems.Selection of measured information is finished by original numeric criterion of observation level of state vector components.Prediction is realized by algorithm of self-organization that makes synthesis of the optimal complication.Therefore mechanism of self-regulation is realized and accuracy of the selective navigation complex is increased.
基金Project (No. NCET-04-0545) supported by the "New Century Elitist Supporting Plan" Fund Project of Education Ministry of China
文摘With the analysis on regulating system in 200 MW steam turbine, the necessity of appending the fast-opening function to the original system is set forth and a new type of fast-opening mechanism is devised. The mathematical model of system is built up. With the use of AMESIM software, the displacement curve of the piston, the force curve of the cartridge valve spool, the pressure curve and the flux curve in the regulation process are obtained based on simulation. The performances of three fast-opening systems composed of cartridge valves with different diameters are compared. Based on the analysis on factors that affect the execution time of fast-opening, the dead zone of the fast-opening system is put forward. To overcome the defect, dif- ferent operation modes are adopted for different zones. The result shows that with the increase of the valve diameter, the regulating time in the dead zone significantly exceeds the fast-opening time in the whole journey. Accordingly, the optimization operation tactic in the dead zone and the qualification conditions are brought forward. The fast-opening system composed of 32 mm cartridge valves is taken as an example with use of the tactic. The simulation result shows that the maximum regulating time is shortened by 509 ms.
基金Project 2005A030 supported by the Youth Science and Research Foundation from China University of Mining & Technology
文摘Camera calibration is a critical process in photogrammetry and a necessary step to acquire 3D information from a 2D image. In this paper, a flexible approach for CCD camera calibration using 2D direct linear transformation (DLT) and bundle adjustment is proposed. The proposed approach assumes that the camera interior orientation elements are known, and addresses a new closed form solution in planar object space based on homogenous coordinate representation and matrix factorization. Homogeneous coordinate representation offers a direct matrix correspondence between the parameters of the 2D DLT and the collinearity equation. The matrix factorization starts by recovering the elements of the rotation matrix and then solving for the camera position with the collinearity equation. Camera calibration with high precision is addressed by bundle adjustment using the initial values of the camera orientation elements. The results show that the calibration precision of principal point and focal length is about 0.2 and 0.3 pixels respectivelv, which can meet the requirements of close-range photogrammetry with high accuracy.
文摘The issue of employer branding has always had an intrinsic interdisciplinary content, since it builds a bridge for cross-fertilization between different disciplines (strategic marketing, strategic management, and human resources management). This paper presents employer branding as an approach based on effective strategic organizational resources and a precise employer branding management process. In this paper, the target is to define a possible frame in order to interpret employer branding techniques as control and regulation mechanisms. Control in organizations has long been a topic of interest for researchers and practitioners, alike who generally recognize that control mechanisms are needed to ensure that organizations may achieve their goals. It has been carried out a field work on the Italian Aeronautical Meta district, that generates annual revenues equal to EUR 8.7 billion and employees 36,300 people, of whom approximately 10% are employed in the space sector. Through this field work, the intention aim is to understand if and how employer branding may create, enforce, and set up internal and shared meaning and values. In particular, stemming from the empirical research's evidences, this paper tries to conceptualize employer branding as a factor to persuade and influence the way that organizational actors enact in a socially constructed context. In this effort, the aim is to understand how managers may affect meanings, values goals, and opinions through an effective action on employer branding that can play a crucial role in affecting sense-making processes, meanings, interpretations of the symbolic environment. The paper therefore opens up to new horizons, since it embraces a new application for employer branding, considering it as a modern control system and proposing an innovative approach in managerial control, founded on organizational identity as a key concept in an organizational citizenship's perspective
基金supported by the National Natural Science Foundation of China(Grant Nos.81171653 and 30972703)Natural Science Foundation of Jiangsu Province(Grant Nos.BK2011246 and BK2011247)Jiangsu Provincial Innovation Award BC2012093 by the Bureau of Science and Technology of Jiangsu Province
文摘MicroRNAs (miRNAs) are small, non-coding single-stranded RNAs that can modulate target gene expression at post- transcriptional level and participate in cell proliferation, differentiation, and apoptosis. T cells have important functions in acquired immune response; miRNAs regulate this immune response by targeting the mRNAs of genes involved in T cell developmentp proliferationj differentiationp and function. For instancep miR-181 family members function in progression by targeting Bcl2 and CD69, among others. MiR-17 to miR-92 clusters function by binding to CREB 1, PTEN, and Bim. Considering that the suppression ofT cell-mediated immune responses against tumor cells is involved in cancer progression, we should investigate the mechanism by which miRNA regulates T cells to develop new approaches for cancer treatment.
文摘AIM:To investigate the pathway(s)mediating rat antral circular smooth muscle contractile responses to the cholinomimetic agent,bethanechol and the subtypes of muscarinic receptors mediating the cholinergic contraction. METHODS:Circular smooth muscle strips from the antrum of Sprague-Dawley rats were mounted in muscle baths in Krebs buffer.Isometric tension was recorded.Cumulative concentration-response curves were obtained for(+)-cis- dioxolane(cD),a nonspecific muscarinic agonist,at 10^(-8)- 10^(-4)mol/L,in the presence of tetrodotoxin(TTX,10^(-7)mol/L). Results were normalized to cross sectional area.A repeat concentration-response curve was obtained after incubation of the muscle for 90 min with antagonists for M1(pirenzepine), M2(methoctramine)and M3(darifenadn)muscarinic receptor subtypes.The sensitivity to PTX was tested by the ip injection of 100 mg/kg of PTX 5 d before the experiment.The antral circular smooth muscles were removed from PTX-treated and non-treated rats as strips and dispersed smooth muscle cells to identify whether PTX-linked pathway mediated the contractility to bethanechol. RESULTS:A dose-dependent contractile response observed with bethanechol,was not affected by TTx.The pretreatment of rats with pertussis toxin decreased the contraction induced by bethanechol.Lack of calcium as well as the presence of the L-type calcium channel blocker,nifedipine,also inhibited the cholinergic contraction,with a reduction in response from 2.5±0.4 g/mm^2 to 1.2±0.4 g/mm^2(P<0.05).The dose- response curves were shifted to the right by muscarinic antagonists in the following order of affinity:darifenacin (M_3)>methocramine(M_2)>pirenzepine(M_1). CONCLUSION:The muscarinic receptors-dependent contraction of rat antral circular smooth muscles was linked to the signal transduction pathway(s)involving pertussis-toxin sensitive GTP-binding proteins and to extracellular calcium via L-type voltage gated calcium channels.The presence of the residual contractile response after the treatment with nifedipine,suggests that an additional pathway could mediate the cholinergic contraction.The involvement of more than one muscarinic receptor(functionally predominant type 3 over type 2)also suggests more than one pathway mediating the cholinergic contraction in rat antrum.