微生物添加剂和中药调节针剂喂猪试验

本试验选择杜双沙三元杂交猪45头,随机分成5组(对照组、微生物组、预混料组、能博万A组、能博万B组),进行饲养试验及屠宰测定。结果表明,复合微生物添加剂与能博万中药调节针剂联合应用于生长育肥猪,能获得市售抗生素预混料同样的效果。能博万B组、A组日增重分别为749.22g、724.16g,料肉比分别为2.84∶1、2.92∶1,与预混料组基本相同;瘦肉率能博万A组达63.47%,高于对照组3.24个百分点,高于预混料组2.33个百分点,能博万B组则分别高于对照组、预混料组2.28、1.37个百分点。综合比较,能博万B组优于能博万A组,因此,实际生产中宜推广微生物添加剂加50mL能博万中药调节针剂组合。

兽用中药调节针剂对育肥猪肝脏 肌肉 肾脏中微量元素影响的分析研究

选择健康双月龄三元杂交仔猪 4 0头 ,体重约 2 0kg ,随机分为 5组 ,对照Ⅰ组饲喂标准日粮 ,对照Ⅱ组饲喂推荐日粮 ,试验Ⅰ、Ⅱ、Ⅲ组饲喂推荐日粮并注射中药调节针剂 ,注射剂量分别为 5 0、30、70ml,试验期为12 0d ,试验结束后对肌肉及内脏器官中微量元素及有害元素进行分析 ,了解中药调节针剂的使用对内脏器官的影响。

中药调节针剂对猪血液生化指标变化规律的研究

仔猪使用中药调节针剂后 ,通过三次不同时间采血 ,用放射免疫分析法 ,检测血液中T3 、T4、CSF。结果表明 ,使用中药调节针剂后 ,仔猪血液中T3 、T4、CSF有不同程度变化 。

Effects of electroacupuncture on the behaviors and expressions of hippocampal neurotransmitters and Bax/Bcl-2 proteins in rat models of anxiety disorder

Objective:To investigate the effects of electroacupuncture(EA)on the behaviors of rat with anxiety disorder,and the expressions of hippocampal neurotransmitters including 5-hydroxytryptamine(5-HT),norepinephrine(NE)and dopamine(DA),and the expressions of hippocampal B-cell lymphoma-2(Bcl-2)and Bcl-2 associated X(Bax).Methods:Forty-six male Wistar rats were randomly divided into a control group(n=10);a model group(n=12),an EA group(n=12),and a drug group(n=12).Except the control group,the other three groups were established into rat models of anxiety disorder using uncertain empty bottle stimulation.Rats in the EA group and the drug group received corresponding interventions for 15 consecutive days[EA group was given EA at Baihui(GV 20)and Sanyinjiao(SP 6);the drug group was given aqueous solution of alprazolam via intragastric administration].After intervention,all four groups received open-field test(OFT)and elevated plus-maze(EPM)for behavioral evaluations.The expressions of 5-HT,NE and DA in hippocampus were determined by fluorescence spectroscopy(FS)while the expressions of Bcl-2 and Bax proteins in hippocampus were determined by Western blot(WB).Results:The OFT horizontal scores in the control group,EA group and drug group were significantly higher than that in the model group(all P<0.05),and the difference between the EA group and the drug group was statistically insignificant(P>0.05);the OFT vertical scores in the model group,EA group and drug group were significantly lower than the score in the control group(all P<0.05).The EPM percent of open-arm entries(OE%)in the control group,EA group and drug group was higher than that in the model group(P<0.05),and the differences among these three groups were statistically insignificant(P>0.05);though the percent of open-arm total time(OT%)in the EA group was lower than that in the control group(P<0.05);the difference was statistically insignificant when compared with the drug group(P>0.05),and it was significantly higher than that in the model group(P<0.05).The expression of 5-HT in the EA group was higher than that in the control group(P<0.05);the expression of 5-HT in the EA group was significantly lower than that in the model group(P<0.05);the difference between the EA group and the drug group was statistically insignificantly(P>0.05).The expression of NE in the model group was significantly higher than that in the other three groups(P<0.0S),and there was no significant difference among these three groups(P>0.05).The expression of DA in the EA group was significantly higher than that in the control group and the drug group(both P<0.05),while the difference between the EA group and the model group was statistically insignificant(P>0.05).The expression of Bax in the model group was significantly higher than that in the other three groups(all P<0.05),whereas the expression of Bcl-2 in the model group was significantly lower than that in the other three groups(all P<0.05),and the differences in both Bax and Bcl-2 among the other three groups were statistically insignificant(all P>0.05).Bax/Bcl-2 in the EA group was significantly higher than that in the control group(P<0.05)and lower than that in the model group(P<0.05),and the difference was statistically insignificant when compared with the drug group(P>0.05).Conclusion:EA shows promising effects in attenuating rats'anxiety disorder,which may be achieved by the down-regulation of the expressions of 5-HT and NE in the hippocampus and/or inhibition of hippocampal neuronal apoptosis.The efficacy is comparable to that of intervention with alprazolam.