[Objective] The aim was to research effects of bamboo split curing method and steely barn curing method on quality of yellow sun-cured tobacco, providing scientific references for optimization of curing technology of ...[Objective] The aim was to research effects of bamboo split curing method and steely barn curing method on quality of yellow sun-cured tobacco, providing scientific references for optimization of curing technology of yellow sun-cured tobacco. [method] The tobaccos were divided into two groups treated with two methods and the related indices of treated tobaccos were then measured to evaluate effects of curing methods on quality of yellow sun-cured tobacco. [Result] Tobaccos treated with two methods were all dominated by reddish yellow and yellow; exterior quality differed little. In detail, total sugar and reducing sugar in an average level in three locations were all higher with method A than those with method B, but nicotine and total N were lower; comprehensive chemical components in group with method B proved more appropriate with higher K. In addition, sensory qualities of leaves in moderate and bottom locations were better with method B compared with method A; sensory quality of top leaves was better; comprehensive sensory quality of leaves treated with method B proved better. On the other hand, cost for method A was 2.82 times of method B; the area of sun-curing field was 3.27 times and manage- ment work was 1.77 times, which indicated that method B is low-cost and time-saving, which would be a guarantee of tobacco quality. [Conclusion] The research provides scientific references for rational use of tobacco resources and optimization of sun-curing technology.展开更多
The molecular modifications of Herpes Simplex Virus Type I （HSV-1） proteins represented by acetylation and phosphorylation are essential to its biological functions. The cellular chromatin-remodeling/ assembly is in...The molecular modifications of Herpes Simplex Virus Type I （HSV-1） proteins represented by acetylation and phosphorylation are essential to its biological functions. The cellular chromatin-remodeling/ assembly is involved in HSV-1 associated gene transcriptional regulation in human cells harboring HSV-1 lytic or latent infections. Further investigation on these biological events would provide a better understanding of the mechanisms of HSV-1 viral gene transcriptional regulation展开更多
The effects of five domestic cooking methods,including steaming,microwaving,boiling,stir-frying,and stir-frying followed by boiling(stir-frying/boiling),on the nutrients and health-promoting compounds of broccoli were...The effects of five domestic cooking methods,including steaming,microwaving,boiling,stir-frying,and stir-frying followed by boiling(stir-frying/boiling),on the nutrients and health-promoting compounds of broccoli were investigated.The results show that all cooking treatments,except steaming,caused significant losses of chlorophyll and vitamin C and significant decreases of total soluble proteins and soluble sugars.Total aliphatic and indole glucosinolates were significantly modified by all cooking treatments but not by steaming.In general,the steaming led to the lowest loss of total glucosinolates,while stir-frying and stir-frying/boiling presented the highest loss.Stir-frying and stir-frying/boiling,the two most popular methods for most homemade dishes in China,cause great losses of chlorophyll,soluble protein,soluble sugar,vitamin C,and glucosinolates,but the steaming method appears the best in retention of the nutrients in cooking broccoli.展开更多
The aim of this study was to establish a quality-control method for calcineurin subunit B(CNB) biological activity determinations. CNB enhances the p-nitrophenylphosphate(p NPP) dephosphorylating activity of calcineur...The aim of this study was to establish a quality-control method for calcineurin subunit B(CNB) biological activity determinations. CNB enhances the p-nitrophenylphosphate(p NPP) dephosphorylating activity of calcineurin subunit A Δ316 mutant(CNAΔ316). A series of CNB concentrations were fitted to a four-parameter equation to calculate the corresponding p NPP maximum dephosphorylation rates. Values were calculated based on biological activity references using a parallel line method. The method was then validated for accuracy, precision, linearity, linear range, sensitivity, specificity, and robustness. The recovery results were greater than 98%. Intra-plate precision was 6.7%, with inter-plate precision of 10.8%. The coefficient of determination was greater than 0.98. The linear range was 0.05–50 μg m L?1, with sensitivity of 50 μg m L?1. Tested cytokines did not induce CNAΔ316 dephosphorylation of p NPP. The chosen CNAΔ316 concentration range did not affect activity determinations.展开更多
基金Supported by China Tobacco Yunnan Indutrial Co.Ltd Scientific Program(2010YL02)~~
文摘[Objective] The aim was to research effects of bamboo split curing method and steely barn curing method on quality of yellow sun-cured tobacco, providing scientific references for optimization of curing technology of yellow sun-cured tobacco. [method] The tobaccos were divided into two groups treated with two methods and the related indices of treated tobaccos were then measured to evaluate effects of curing methods on quality of yellow sun-cured tobacco. [Result] Tobaccos treated with two methods were all dominated by reddish yellow and yellow; exterior quality differed little. In detail, total sugar and reducing sugar in an average level in three locations were all higher with method A than those with method B, but nicotine and total N were lower; comprehensive chemical components in group with method B proved more appropriate with higher K. In addition, sensory qualities of leaves in moderate and bottom locations were better with method B compared with method A; sensory quality of top leaves was better; comprehensive sensory quality of leaves treated with method B proved better. On the other hand, cost for method A was 2.82 times of method B; the area of sun-curing field was 3.27 times and manage- ment work was 1.77 times, which indicated that method B is low-cost and time-saving, which would be a guarantee of tobacco quality. [Conclusion] The research provides scientific references for rational use of tobacco resources and optimization of sun-curing technology.
基金National Natural Science Foundation of China (30670094,30700028)
文摘The molecular modifications of Herpes Simplex Virus Type I （HSV-1） proteins represented by acetylation and phosphorylation are essential to its biological functions. The cellular chromatin-remodeling/ assembly is involved in HSV-1 associated gene transcriptional regulation in human cells harboring HSV-1 lytic or latent infections. Further investigation on these biological events would provide a better understanding of the mechanisms of HSV-1 viral gene transcriptional regulation
基金supported by the High-Tech R&D Program (863) of China (No.2008AA10Z111)the National Natural Science Foundation of China (No.30320974)+1 种基金the Fok Ying Tong Education Foundation of China (No.104034)the Program for New Century Excellent Talents in the University of China (No.NCET-05-0516)
文摘The effects of five domestic cooking methods,including steaming,microwaving,boiling,stir-frying,and stir-frying followed by boiling(stir-frying/boiling),on the nutrients and health-promoting compounds of broccoli were investigated.The results show that all cooking treatments,except steaming,caused significant losses of chlorophyll and vitamin C and significant decreases of total soluble proteins and soluble sugars.Total aliphatic and indole glucosinolates were significantly modified by all cooking treatments but not by steaming.In general,the steaming led to the lowest loss of total glucosinolates,while stir-frying and stir-frying/boiling presented the highest loss.Stir-frying and stir-frying/boiling,the two most popular methods for most homemade dishes in China,cause great losses of chlorophyll,soluble protein,soluble sugar,vitamin C,and glucosinolates,but the steaming method appears the best in retention of the nutrients in cooking broccoli.
基金supported by the National Important Novel Medicine Research Project (2012ZX09304010, 2013ZX09102062)the National Natural Science Foundation of China (31270849)
文摘The aim of this study was to establish a quality-control method for calcineurin subunit B(CNB) biological activity determinations. CNB enhances the p-nitrophenylphosphate(p NPP) dephosphorylating activity of calcineurin subunit A Δ316 mutant(CNAΔ316). A series of CNB concentrations were fitted to a four-parameter equation to calculate the corresponding p NPP maximum dephosphorylation rates. Values were calculated based on biological activity references using a parallel line method. The method was then validated for accuracy, precision, linearity, linear range, sensitivity, specificity, and robustness. The recovery results were greater than 98%. Intra-plate precision was 6.7%, with inter-plate precision of 10.8%. The coefficient of determination was greater than 0.98. The linear range was 0.05–50 μg m L?1, with sensitivity of 50 μg m L?1. Tested cytokines did not induce CNAΔ316 dephosphorylation of p NPP. The chosen CNAΔ316 concentration range did not affect activity determinations.
