Precise structural identification of phospholipids in the microalga Nitzschia closterium has been established using ultra performance liquid chromatography-electrospray ionization-quadrupole-time of flight-mass spectr...Precise structural identification of phospholipids in the microalga Nitzschia closterium has been established using ultra performance liquid chromatography-electrospray ionization-quadrupole-time of flight-mass spectrometry (UPLC-ESI-Q-TOF-MS) for direct analysis of total lipid extracts.Mass spectrometry was performed in reflective time-of-flight using electron spraying ionization in negative mode.Phospholipid molecular species identification was based on the characteristic product ions and neutral loss yielded by different phospholipids under ESI-MS/MS mode.The molecular species were confirmed by the carboxylate anions produced by phospholipids in negative mode;the regiospecificity of the two acyl chains was determined from the ratio of sn-1 to sn-2 carboxylate anion abundances.As a result,18 lipid molecular species were identified for the first time in this microalga,comprising seven phosphatidylcholines (PC),two phosphatidylethanolamines (PE),two phosphatidylinositols (PI),and seven phosphatidylglycerols (PG).Lipid standards of PC,PE,PI,and PG were added to the total lipids as internal standards for semiquantitative analysis,revealing concentrations of phospholipids in this species between 0.09 and 3.37 nmol/mg.This method can produce a full structural profile of intact phospholipid molecular species and can be used for study of the physiological and ecological functions of lipids by monitoring their individual changes over time.展开更多
Chinese universities are facing a series of challenges in their accommodations, of which a predominant one is how to provide both students and staff with enough accommodations of a large scope and good quality in good...Chinese universities are facing a series of challenges in their accommodations, of which a predominant one is how to provide both students and staff with enough accommodations of a large scope and good quality in good time and with limited budgets. In order to find applicable approaches to solve this problem, the current situations of the Chinese higher education reform and the resulted rapid growth of student numbers whereby the requirement of lodgings is greatly increased are discussed. It is elucidated that in providing enough accommodations for staff and students, Chinese universities are confronted with difficulties including demand too big, lack of funding support, time limitation and incapability in both project management and facilities management. Industrialized building approach is demonstrated to be a desirable access to build large quantities of accommodations of good quality in limited time, and Private Financial Initiative (PFI) appears to be a prosperous solution to the fund shortage. Regarding project and facilities management, qualified professionals are available by either employing exterior consultants or training related staff of universities for such occupations.展开更多
Return period is generally adopted to calculate the design value of environmental condition in offshore structure design. However, it can not make relevant adjustment according to structure＇s, especially the mobile u...Return period is generally adopted to calculate the design value of environmental condition in offshore structure design. However, it can not make relevant adjustment according to structure＇s, especially the mobile unit＇s, life time or its operation areas and usually make the design either insufficiently safe or rather uneconomical. A formula is developed to solve this problem in the case of the design wave height, where the risk, the design life, the distributions of wave heights in operation areas and the operating durations in each area are regarded as parameters. The applications of this method and the comparisons with the general method are presented. The result of this method is considered to be proper.展开更多
基金Supported by the Program for Changjiang Scholars and Innovative Research Team in University (PCSIRT) (No. IRT0734)Zhejiang Provincial Natural Science Foundation (No. Y506131)+1 种基金National Key Technology Research and Development Program (No. 2007BAD43B09)K. C. Wong Magna Fund of Ningbo University
文摘Precise structural identification of phospholipids in the microalga Nitzschia closterium has been established using ultra performance liquid chromatography-electrospray ionization-quadrupole-time of flight-mass spectrometry (UPLC-ESI-Q-TOF-MS) for direct analysis of total lipid extracts.Mass spectrometry was performed in reflective time-of-flight using electron spraying ionization in negative mode.Phospholipid molecular species identification was based on the characteristic product ions and neutral loss yielded by different phospholipids under ESI-MS/MS mode.The molecular species were confirmed by the carboxylate anions produced by phospholipids in negative mode;the regiospecificity of the two acyl chains was determined from the ratio of sn-1 to sn-2 carboxylate anion abundances.As a result,18 lipid molecular species were identified for the first time in this microalga,comprising seven phosphatidylcholines (PC),two phosphatidylethanolamines (PE),two phosphatidylinositols (PI),and seven phosphatidylglycerols (PG).Lipid standards of PC,PE,PI,and PG were added to the total lipids as internal standards for semiquantitative analysis,revealing concentrations of phospholipids in this species between 0.09 and 3.37 nmol/mg.This method can produce a full structural profile of intact phospholipid molecular species and can be used for study of the physiological and ecological functions of lipids by monitoring their individual changes over time.
文摘Chinese universities are facing a series of challenges in their accommodations, of which a predominant one is how to provide both students and staff with enough accommodations of a large scope and good quality in good time and with limited budgets. In order to find applicable approaches to solve this problem, the current situations of the Chinese higher education reform and the resulted rapid growth of student numbers whereby the requirement of lodgings is greatly increased are discussed. It is elucidated that in providing enough accommodations for staff and students, Chinese universities are confronted with difficulties including demand too big, lack of funding support, time limitation and incapability in both project management and facilities management. Industrialized building approach is demonstrated to be a desirable access to build large quantities of accommodations of good quality in limited time, and Private Financial Initiative (PFI) appears to be a prosperous solution to the fund shortage. Regarding project and facilities management, qualified professionals are available by either employing exterior consultants or training related staff of universities for such occupations.
基金the National Great Project of Scientific and Technical Supporting Programs Funded by Ministry of Science & Technology of China During the 11th Five-year Plan (No. 2006BAA01A25)
文摘Return period is generally adopted to calculate the design value of environmental condition in offshore structure design. However, it can not make relevant adjustment according to structure＇s, especially the mobile unit＇s, life time or its operation areas and usually make the design either insufficiently safe or rather uneconomical. A formula is developed to solve this problem in the case of the design wave height, where the risk, the design life, the distributions of wave heights in operation areas and the operating durations in each area are regarded as parameters. The applications of this method and the comparisons with the general method are presented. The result of this method is considered to be proper.
