[目的]调查钻井工人体质指数(body mass index,BMI)与血压、血脂、谷丙氨酸氨基转移酶(alaninetransaminase,ALT)、脂肪肝水平的关系,为改变其不良生活习惯,提高健康水平提供理论依据。[方法]对胜利油田927名钻井工人的体检资料进行分析...[目的]调查钻井工人体质指数(body mass index,BMI)与血压、血脂、谷丙氨酸氨基转移酶(alaninetransaminase,ALT)、脂肪肝水平的关系,为改变其不良生活习惯,提高健康水平提供理论依据。[方法]对胜利油田927名钻井工人的体检资料进行分析,根据年龄、工龄、BMI的不同划分范围各分为3组,分别比较3组之间血压、血清ALT、血脂以及脂肪肝等异常结果的发生率。[结果]胜利油田钻井工人超重、肥胖和血压、血清ALT、血脂异常及脂肪肝的发生率分别为39.27%、18.66%、30.85%、9.28%、39.59%、37.65%。血压、血清ALT、血脂异常及脂肪肝的发生率均随着年龄、工龄、BMI的增长而升高,差异有统计学意义。[结论]胜利油田钻井工人的高血压、高血脂、血清ALT水平的升高及脂肪肝的患病率均随着年龄、工龄与BMI的增长而升高,且超重组与肥胖组的患病率均显著高于BMI正常组。展开更多
An electrochemiluminescent (ECL) biosensor was constructed for selective assay of alanine aminotransferase (ALT) based on the enzymatically catalyzed oxidation of pyruvate by pyruvate oxidase (PYOD). The composite of ...An electrochemiluminescent (ECL) biosensor was constructed for selective assay of alanine aminotransferase (ALT) based on the enzymatically catalyzed oxidation of pyruvate by pyruvate oxidase (PYOD). The composite of potassium ferricyanide and carbon nanotube was adopted to pre-functionalize the basal platinum electrode while the potassium ferricyanide acted as the activator of PYOD. The ALT catalyzed the reaction of L-alanine and-ketoglutarate to produce pyruvate which could be further enzymatically oxidized by PYOD to yield H2O2 to intensify the ECL of luminol. The biosensor showed rapid response for real-time measurement of ALT in the linear concentration range from 0.00475 to 350 U/L (r = 0.993) with a relatively standard deviation of 2.5% (CALT = 47.5 U/L,n = 6). The biosensor was applied to assay the ALT in rat serum with average recovery of 90.5%.展开更多
文摘[目的]调查钻井工人体质指数(body mass index,BMI)与血压、血脂、谷丙氨酸氨基转移酶(alaninetransaminase,ALT)、脂肪肝水平的关系,为改变其不良生活习惯,提高健康水平提供理论依据。[方法]对胜利油田927名钻井工人的体检资料进行分析,根据年龄、工龄、BMI的不同划分范围各分为3组,分别比较3组之间血压、血清ALT、血脂以及脂肪肝等异常结果的发生率。[结果]胜利油田钻井工人超重、肥胖和血压、血清ALT、血脂异常及脂肪肝的发生率分别为39.27%、18.66%、30.85%、9.28%、39.59%、37.65%。血压、血清ALT、血脂异常及脂肪肝的发生率均随着年龄、工龄、BMI的增长而升高,差异有统计学意义。[结论]胜利油田钻井工人的高血压、高血脂、血清ALT水平的升高及脂肪肝的患病率均随着年龄、工龄与BMI的增长而升高,且超重组与肥胖组的患病率均显著高于BMI正常组。
基金supported by the National Natural Science Foundation of China (20275025 & 20675055)the Natural Science Fundation of Jiangsu Province (BK2009111)Technology Plan of Suzhou (SYJG0901)
文摘An electrochemiluminescent (ECL) biosensor was constructed for selective assay of alanine aminotransferase (ALT) based on the enzymatically catalyzed oxidation of pyruvate by pyruvate oxidase (PYOD). The composite of potassium ferricyanide and carbon nanotube was adopted to pre-functionalize the basal platinum electrode while the potassium ferricyanide acted as the activator of PYOD. The ALT catalyzed the reaction of L-alanine and-ketoglutarate to produce pyruvate which could be further enzymatically oxidized by PYOD to yield H2O2 to intensify the ECL of luminol. The biosensor showed rapid response for real-time measurement of ALT in the linear concentration range from 0.00475 to 350 U/L (r = 0.993) with a relatively standard deviation of 2.5% (CALT = 47.5 U/L,n = 6). The biosensor was applied to assay the ALT in rat serum with average recovery of 90.5%.
