In rice (Oryza sativa L.) roots two glutamine synthetase (GS) isozymes, GSra and GSrb, were identified recently in the author's experiments, but the homology of both GSra and GSrb as well as their localization in ...In rice (Oryza sativa L.) roots two glutamine synthetase (GS) isozymes, GSra and GSrb, were identified recently in the author's experiments, but the homology of both GSra and GSrb as well as their localization in the rice roots are unclear. In the present study, the purified GSra and GSrb from rice roots were used to immunize rabbits to obtain the respective antibodies. The immunodiffusion and immunoblotting experiments showed that the antibody against GSra or GSrb was specific for GS and its isozymes. The immunoprecipitation test indicated that the antibody of GSra or GSrb not only recognized its respective antigen, but also well recognized each other's antigen. GSra or GSrb antibody recognized also better cytosolic GS1 of rice leaves, but the recognization for chloroplast GS2 from rice or spinach (Spinacia oleracea Mill.) leaves was weaker. Our results indicate that GSra and GSrb from rice roots are quite similar in antigenicity and are extremely similar proteins and that both GSra and GSrb may also be a form of cytosolic GS just as the cytosolic GS1 of rice leaves.展开更多
Flood land, with abundant light, heat, and water resource, is an important potential land resource to resolve the situation of the limited arable land in mountain areas in Xiaojiang Valley, Dongchuan, Yunnan. It will ...Flood land, with abundant light, heat, and water resource, is an important potential land resource to resolve the situation of the limited arable land in mountain areas in Xiaojiang Valley, Dongchuan, Yunnan. It will have a great significance on the development of local characteristic agriculture and the settlement of poor moun- tain immigrants that how to develop and protect the flood land in a scientific, effec- tive and sustainable way. In this paper, flood land of Xiaojiang Valley is divided into three zones （higher zone is 1 600-1 300 m, medium zone is 1 300-1 000 m, low- er zone is 1 000-700 m） according to different elevations. Through field survey, in situ measurement, sampling analysis and ＂3S＂ technology, the area and soil nutri- ent characteristics of flood land at different altitudes are tested and analyzed sys- tematically. And also according to the climatic characteristics in different elevations of flood land, the research analyzed and investigated the growing conditions of flood land in different elevations in Xiaojiang Valley, and then discussed how to develop and cultivate reasonably and effectively in flood land. All the work is intended to provide beneficial reference for the development and cultivation of flood land in mountain areas.展开更多
New oxygen and hydrogen isotope ratios of chert from middle, intraformational breccias, and upper breccia members of the Sixtymile Formation（SMF） in eastern Grand Canyon National Park（AZ） yield palaeoclimate estim...New oxygen and hydrogen isotope ratios of chert from middle, intraformational breccias, and upper breccia members of the Sixtymile Formation（SMF） in eastern Grand Canyon National Park（AZ） yield palaeoclimate estimates between 27 and 33℃. The isotopic compositions of cherts define a domain approximately parallel to the meteoric water line when plotted on a δD–δ-（18）O diagram; these data indicate that meteoric water was involved during formation of the chert. In thin section, the absence of interlocking mega quartz（〉35 lm） and silicafilled fractures and veins, along with preserved micromorphological silica fabrics, suggest that the chert has not been permeated by later hydrothermal fluids. Petrographic observations in thin section such as cyclic silica precipitation phases and glaebular micromorphologic fabrics lend support to the interpretation that meteoric waters were involved during chert precipitation. The post 742 Ma SMF has been correlated with diamictite（transition） beds of the Kingston Peak Formation（CA）, which in turn have been interpreted to have been deposited during the Sturtian Ice Age（-750–700 Ma）. Absence of facetted and striated clasts and other diagnostic glaciogenic features in the SMF,an unconformable contact with the stratigraphically older Chuar Group, coupled with warm palaeotemperature data inferred from stable isotope values of chert, tentatively suggest that deposition of sediment in the SMF likely did not take place during the Sturtian Ice Age.展开更多
文摘In rice (Oryza sativa L.) roots two glutamine synthetase (GS) isozymes, GSra and GSrb, were identified recently in the author's experiments, but the homology of both GSra and GSrb as well as their localization in the rice roots are unclear. In the present study, the purified GSra and GSrb from rice roots were used to immunize rabbits to obtain the respective antibodies. The immunodiffusion and immunoblotting experiments showed that the antibody against GSra or GSrb was specific for GS and its isozymes. The immunoprecipitation test indicated that the antibody of GSra or GSrb not only recognized its respective antigen, but also well recognized each other's antigen. GSra or GSrb antibody recognized also better cytosolic GS1 of rice leaves, but the recognization for chloroplast GS2 from rice or spinach (Spinacia oleracea Mill.) leaves was weaker. Our results indicate that GSra and GSrb from rice roots are quite similar in antigenicity and are extremely similar proteins and that both GSra and GSrb may also be a form of cytosolic GS just as the cytosolic GS1 of rice leaves.
基金Supported by National Technology R&D Program(0BAC06B02)~~
文摘Flood land, with abundant light, heat, and water resource, is an important potential land resource to resolve the situation of the limited arable land in mountain areas in Xiaojiang Valley, Dongchuan, Yunnan. It will have a great significance on the development of local characteristic agriculture and the settlement of poor moun- tain immigrants that how to develop and protect the flood land in a scientific, effec- tive and sustainable way. In this paper, flood land of Xiaojiang Valley is divided into three zones （higher zone is 1 600-1 300 m, medium zone is 1 300-1 000 m, low- er zone is 1 000-700 m） according to different elevations. Through field survey, in situ measurement, sampling analysis and ＂3S＂ technology, the area and soil nutri- ent characteristics of flood land at different altitudes are tested and analyzed sys- tematically. And also according to the climatic characteristics in different elevations of flood land, the research analyzed and investigated the growing conditions of flood land in different elevations in Xiaojiang Valley, and then discussed how to develop and cultivate reasonably and effectively in flood land. All the work is intended to provide beneficial reference for the development and cultivation of flood land in mountain areas.
基金provided by a grant from Chuck Baltzer,Environmental Support Servicesgraciously awarded by Grand Canyon National Park officials
文摘New oxygen and hydrogen isotope ratios of chert from middle, intraformational breccias, and upper breccia members of the Sixtymile Formation（SMF） in eastern Grand Canyon National Park（AZ） yield palaeoclimate estimates between 27 and 33℃. The isotopic compositions of cherts define a domain approximately parallel to the meteoric water line when plotted on a δD–δ-（18）O diagram; these data indicate that meteoric water was involved during formation of the chert. In thin section, the absence of interlocking mega quartz（〉35 lm） and silicafilled fractures and veins, along with preserved micromorphological silica fabrics, suggest that the chert has not been permeated by later hydrothermal fluids. Petrographic observations in thin section such as cyclic silica precipitation phases and glaebular micromorphologic fabrics lend support to the interpretation that meteoric waters were involved during chert precipitation. The post 742 Ma SMF has been correlated with diamictite（transition） beds of the Kingston Peak Formation（CA）, which in turn have been interpreted to have been deposited during the Sturtian Ice Age（-750–700 Ma）. Absence of facetted and striated clasts and other diagnostic glaciogenic features in the SMF,an unconformable contact with the stratigraphically older Chuar Group, coupled with warm palaeotemperature data inferred from stable isotope values of chert, tentatively suggest that deposition of sediment in the SMF likely did not take place during the Sturtian Ice Age.
