Characteristics and antioxidant activities of pepsin-soluble collagen （PSC） from yellow goosefish （Lophius litulon） skins were investigated. PSC was characterized as a type I collagen, and its imino acid content w...Characteristics and antioxidant activities of pepsin-soluble collagen （PSC） from yellow goosefish （Lophius litulon） skins were investigated. PSC was characterized as a type I collagen, and its imino acid content was 193 residues/1 000 residues. PSC＇s denaturation temperature was -17.56℃ and Fourier transform infrared spectra confirmed the presence of triple helices. Solubility analysis showed good solubility at acidic pH （1-6） or low NaCl concentrations （≤2%）. PSC showed scavenging activity against hydroxyl radicals and superoxide anions in a concentration-dependent manner. Furthermore, PSC could protect D-galactose-induced skin aging by significantly controlling malondialdehyde formation and improving the activity of superoxide dismutase, glutathione peroxidase, catalase, glutathione, and hydroxyproline. PSC may be a promising antioxidant in appropriate applications.展开更多
Objective： To observe the effects of acupuncture and moxibustion on the skin histomorphology of senile mice. Methods： The subacute senile model of mouse was made by subcutaneous injection of D-galactose into the nec...Objective： To observe the effects of acupuncture and moxibustion on the skin histomorphology of senile mice. Methods： The subacute senile model of mouse was made by subcutaneous injection of D-galactose into the neck, then they were randomly allocated into an acupuncture group, a moxibustion group, a model group, and a blank group, and measured the content of malondialdehyde （MDA）, activity of glutathione peroxidase （GSH-Px）, and content of hydroxyproline （Hyp） in the skin tissue. Results： Acupuncture and moxibustion at Zusanli （ST 36） could decrease the content of MDA, enhance the activity of GSH-Px, and increase the content of Hyp in the skin tissue of senile mice. Conclusion： Acupuncture and moxibustion at Zusanli （ST 36） could delay the senescence progress of mice＇s skin.展开更多
基金Supported by the International S&T Cooperation Program of China(No.2015DFA30980)the Zhejiang Provincial Natural Science Foundation of China(No.LQ14C170001)the Special Program for the Science and Technology Plan of Zhejiang Province(No.2011C02003)
文摘Characteristics and antioxidant activities of pepsin-soluble collagen （PSC） from yellow goosefish （Lophius litulon） skins were investigated. PSC was characterized as a type I collagen, and its imino acid content was 193 residues/1 000 residues. PSC＇s denaturation temperature was -17.56℃ and Fourier transform infrared spectra confirmed the presence of triple helices. Solubility analysis showed good solubility at acidic pH （1-6） or low NaCl concentrations （≤2%）. PSC showed scavenging activity against hydroxyl radicals and superoxide anions in a concentration-dependent manner. Furthermore, PSC could protect D-galactose-induced skin aging by significantly controlling malondialdehyde formation and improving the activity of superoxide dismutase, glutathione peroxidase, catalase, glutathione, and hydroxyproline. PSC may be a promising antioxidant in appropriate applications.
基金National Key Laboratory Cultivation of the Laboratory on Combined Acupuncture and Medication,Higher Education of Jiangsu Province
文摘Objective： To observe the effects of acupuncture and moxibustion on the skin histomorphology of senile mice. Methods： The subacute senile model of mouse was made by subcutaneous injection of D-galactose into the neck, then they were randomly allocated into an acupuncture group, a moxibustion group, a model group, and a blank group, and measured the content of malondialdehyde （MDA）, activity of glutathione peroxidase （GSH-Px）, and content of hydroxyproline （Hyp） in the skin tissue. Results： Acupuncture and moxibustion at Zusanli （ST 36） could decrease the content of MDA, enhance the activity of GSH-Px, and increase the content of Hyp in the skin tissue of senile mice. Conclusion： Acupuncture and moxibustion at Zusanli （ST 36） could delay the senescence progress of mice＇s skin.
