[Objective] The research aimed to study the mycorrhizal formation in the offspring tissue culture seedling bottle of Pinus elliottii with Lecanosticta acicola resistance.[Method] The mycorrhizal formation of Pinus ell...[Objective] The research aimed to study the mycorrhizal formation in the offspring tissue culture seedling bottle of Pinus elliottii with Lecanosticta acicola resistance.[Method] The mycorrhizal formation of Pinus elliottii regenerated plant in vitro was studied,and the growth condition of regenerated plant was observed.[Result] The culture substrate and the inoculation amount of mycorrhizal fungi had the big influence on the mycorrhizal formation in vitro.After the root primordia were induced by Pinus elliottii caespitose shoots,it was best to transplant in the perlite substrate and inoculate Pisolithus tinctorius,which was favorable for the mycorrhizal formation.When the two blocks of Pisolithus tinctorius were inoculated in the perlite medium,the mycorrhizal formation rate reached 84.4%.The dichotomous branching short root was the most,and 12.49 roots were formed in every main root.The mycorrhizal formation improved the survival rate of regenerated plant domestication transplantation.The mycorrhizal regenerated plant grew well in the phytotron,and the root system was developed.[Conclusion] The research provided the basis for improving the survival rate of Pinus elliottii tissue culture regenerated plant with Lecanosticta acicola resistance.展开更多
[Objective] This study aimed to investigate the effect of inoculation with various rhizobium strains on growth status, phosphorus uptake, yield and quality of soybean plants. [Method] Using Dongnong 42 and Dongnong 46...[Objective] This study aimed to investigate the effect of inoculation with various rhizobium strains on growth status, phosphorus uptake, yield and quality of soybean plants. [Method] Using Dongnong 42 and Dongnong 46 as experimental soybeans, four treatments were designed, including non-inoculated CK group and three treatment groups inoculated respectively with rhizobium strains R2, R, and Rs, to analysis the effects of rhizobium inoculation on growth status, phosphorus uptake, yield and quality of soybean plants. [Result] Inoculation with different rhizobium strains could significantly increase the biomass and phosphorus content of the roots and shoots of Dongnong 42, to be specific, rhizobium strain R2 showed the best ef- fect, followed by Rs, and those of roots were improved greater than the shoots; in- oculation with rhizobium could significantly increase the yield of Dongnong 42, and R5 showed the best effect, but the yield of Dongnong 46 was decreased; after in- oculated with rhizobium, the protein content and fat content of soybean were signifi- cantly increased, and R2 showed the best effect, but the effects on different varieties varied, to be specific, the protein content of Dongnong 42 was significantly in- creased, while the fat content of Dongnong 46 was significantly increased; different rhizobium strains inoculated to different soybean varieties showed different effects. Inoculation with suitable rhizobium can improve plant growth characteristics, promote phosphorus uptake of plants, increase soybean yield, and effectively improve soy- bean quality. [Conclusion] This study provides theoretical basis for solving the prob- lem of soil phosphorus deficiency, increasing soybean yield and improving soybean quality in the future.展开更多
[Objective] To isolate and preserve a high-activity Douchi fibrinolytic enzyme producing strain from Douchi products. [Method] The Douchi flbrinolytic enzyme producing strain was screened on the selected medium prepar...[Objective] To isolate and preserve a high-activity Douchi fibrinolytic enzyme producing strain from Douchi products. [Method] The Douchi flbrinolytic enzyme producing strain was screened on the selected medium prepared with self-made pork blood powder, the strain with the highest activity was screened out according to the size of hydrolysis cycle, and then preserved in LB medium. [ Rebait] A Douchi fibrinolytic enzyme producing strain with high thrombolytic activity was successfully screened out from the Douchi produced in Hunan, Guangdong and Jiangxi Prov- inces. [ Ceadusioe] The study lays foundation for the development of new-type thrombolytic drugs.展开更多
基金Supported by State Forestry Administration"948"Item(2011-4-69)~~
文摘[Objective] The research aimed to study the mycorrhizal formation in the offspring tissue culture seedling bottle of Pinus elliottii with Lecanosticta acicola resistance.[Method] The mycorrhizal formation of Pinus elliottii regenerated plant in vitro was studied,and the growth condition of regenerated plant was observed.[Result] The culture substrate and the inoculation amount of mycorrhizal fungi had the big influence on the mycorrhizal formation in vitro.After the root primordia were induced by Pinus elliottii caespitose shoots,it was best to transplant in the perlite substrate and inoculate Pisolithus tinctorius,which was favorable for the mycorrhizal formation.When the two blocks of Pisolithus tinctorius were inoculated in the perlite medium,the mycorrhizal formation rate reached 84.4%.The dichotomous branching short root was the most,and 12.49 roots were formed in every main root.The mycorrhizal formation improved the survival rate of regenerated plant domestication transplantation.The mycorrhizal regenerated plant grew well in the phytotron,and the root system was developed.[Conclusion] The research provided the basis for improving the survival rate of Pinus elliottii tissue culture regenerated plant with Lecanosticta acicola resistance.
基金Supported by Doctoral Scientific Research Start-up Project of Mudanjiang Normal University(MSB200912)"12th Five-Year Plan"Educational Science and Research Project of Heilongjiang Association of Higher Education(HGJXH B1110053)Teaching Reform Project of Mudanjiang Normal University(10-XY01067)~~
文摘[Objective] This study aimed to investigate the effect of inoculation with various rhizobium strains on growth status, phosphorus uptake, yield and quality of soybean plants. [Method] Using Dongnong 42 and Dongnong 46 as experimental soybeans, four treatments were designed, including non-inoculated CK group and three treatment groups inoculated respectively with rhizobium strains R2, R, and Rs, to analysis the effects of rhizobium inoculation on growth status, phosphorus uptake, yield and quality of soybean plants. [Result] Inoculation with different rhizobium strains could significantly increase the biomass and phosphorus content of the roots and shoots of Dongnong 42, to be specific, rhizobium strain R2 showed the best ef- fect, followed by Rs, and those of roots were improved greater than the shoots; in- oculation with rhizobium could significantly increase the yield of Dongnong 42, and R5 showed the best effect, but the yield of Dongnong 46 was decreased; after in- oculated with rhizobium, the protein content and fat content of soybean were signifi- cantly increased, and R2 showed the best effect, but the effects on different varieties varied, to be specific, the protein content of Dongnong 42 was significantly in- creased, while the fat content of Dongnong 46 was significantly increased; different rhizobium strains inoculated to different soybean varieties showed different effects. Inoculation with suitable rhizobium can improve plant growth characteristics, promote phosphorus uptake of plants, increase soybean yield, and effectively improve soy- bean quality. [Conclusion] This study provides theoretical basis for solving the prob- lem of soil phosphorus deficiency, increasing soybean yield and improving soybean quality in the future.
文摘[Objective] To isolate and preserve a high-activity Douchi fibrinolytic enzyme producing strain from Douchi products. [Method] The Douchi flbrinolytic enzyme producing strain was screened on the selected medium prepared with self-made pork blood powder, the strain with the highest activity was screened out according to the size of hydrolysis cycle, and then preserved in LB medium. [ Rebait] A Douchi fibrinolytic enzyme producing strain with high thrombolytic activity was successfully screened out from the Douchi produced in Hunan, Guangdong and Jiangxi Prov- inces. [ Ceadusioe] The study lays foundation for the development of new-type thrombolytic drugs.