豆源降血压肽制备及活性评价方法的研究进展

食源性降血压肽比目前使用的合成降血压药物毒副作用小,且更易吸收。我国豆类品种丰富、产量高,豆源降血压肽是作为降血压药物的良好选择。介绍了豆源降血压肽的作用机制、制备及活性评价方法,并对常见豆源降血压肽进行综述及展望。

豆制品中大豆异黄酮的含量比较

目的考察豆源性物质(几种食品及下脚料)的大豆异黄酮的含量。方法将豆源性制品和下脚料干品进行提取、分离,用TLC鉴别后再以染料木素为对照品,在258 nm处用UV法测定含量。结果大豆异黄酮含量依次为:豆水>豆腐>豆粕>豆芽>大豆>豆豉>豆浆>豆渣。结论豆源性下脚料豆水、豆粕和豆渣均含有一定量的大豆异黄酮,尤其豆水和豆粕是很好的大豆异黄酮原料。另外,豆源性食物中大豆异黄酮含量的比较也为人们饮食提供了依据。

Identification of Faba Bean(Vicia faba L.)Germplasm Resources in Hubei Province and Evaluation on Their Main Agronomic Traits

Through field observation and lab analysis, 13 agronomic traits, including plant height and effective branching number per plant, etc., of 207 faba bean （Vicia faba L.） germplasms saved in Crop Middle-term Genebank of Hubei Province were evaluated, moreover, the identification on the disease resistance and the quality was carried out on parts of germplasm resources. The results showed that the faba beangermplasm resources in Hubei Province were abundant, the variation range of agronomic traits like the growth period, plant height and 100-bean weight, etc., was great, and the range of choice was wide. In the aspect of quality, there were 9 germplasms withthe content of crude protein higher than 30%, and the variation range of amylose content in total starch was large, which provided excellent sources of parents for breedinq hi.qh-quality faba bean varieties in Hubei Province.

Research Progress on Germplasm Resources of Common Bean

The origin, classification, utilization and evaluation of germplasm resources, and breeding methods were introduced. The researches of biotic or/and abiotic stress, exploration of genomics and proteomics in common bean in recent years were reviewed in this paper, in order to provide theoretical reference for utilization and innovation of germplasm resources in common bean.

Responses of Membrane Lipid Peroxidation and Endogenous Hormones of Soybean Seedlings to UV-B Radiation and Rare Earth

[ Objective] The aim was to provide strategies for development of rare earth and control of environmental pollution. [ Method] Responses of membrane lipid peroxidation and endogenous hormones of soybean seedlings to UV-B radiation and rare earth were studied through hydroponics in laboratory. [ Result] The results showed that under irradiation of UV-B（ T1-0.15 W/m^2 and T2-0.45 W/m^2）, chlorophyll and indole-3-acetic acid（IAA） contents firstly decreased during the stress phase （1 -5 d） and then increased during the restoration phase （6 -9 d） while contents of malonadialdehyde（MDA） and abscisic acid（ABA） gradually increased during the imposition of UV-B radiation （1 -5 d） and subsequently decreased during recovery from UV-B stress （6 -9 d） . With adding of La （III） with the concentration of 20 mg · L^-1 , the decline/dse trend of chlorophyll, IAA, MDA and ABA contents was slowed down during the stress period while the rise/decline speed was accelerated during the recovery period. [ Conclusion] It suggests that the regulation of La （ III ） on membrane lipid peroxidation and endogenous hormones could increase chlorophyll and IAA contents, improve the metabolism of reactive oxygen species （ ROS）, inhibit membrane lipid peroxidation, decrease the accumulation amount of ABA and alleviate injury of UV-B radiation to soybean seedlings. Further, the protective potential of La （ III ） was better under low UV-B radiation than under high one.

Pedigree and Trait Analysis of Soybean Varieties Registered in Henan Province

To elucidate the genetic relationship of the soybean varieties registered in Henan province, we analyzed the pedigrees, the major agronomic traits, and the eco- nomic traits of 74 soybean varieties registered in Henan province from 1985 to 2012, with reference to experience and technologies beneficial to breeding. The resuits indicated that these varieties originated from 113 parents, and positive correla- tion between the yield and the number of original parents was significant. In the major agronomic traits, the growth stage was about 106 days with relatively small variation, while the final height varied largely among these varieties. Among the fac- tore affecting the final yield, the pod number per plant exhibited an increasing trend, while the variation of 100-grain weight was relatively small. More than 50% of the 113 original parents came from Henan Province and its neighbor provinces, and the genetic relationship among the registered varieties was too close. The rational criteria of yield components of soybean varieties in Henan Province should be as fol- lows： the pod number per plant was about 48. 6, with 100-grain weight ranging from 17.55 g to 21.80 g.

Research Progress of Wild Soybean Germplasms and Utilization

Wild soybean （Glycine soja） is the wild relative species of cultivated soy- bean, which is of high protein content, strong tolerance and high propagation coeffi- cient. The diverse growing conditions of wild soybean in China contribute to numer- ous phenotypes of wild soybean, which also reflect the evolution process through long-term natural and artificial selection. The research progress of wild soybean germpfasms was summarized in resource status and innovative utilization in order to provide information for research of wild soybean germplasms.

Effects of Exogenous Phenolic Acids on Allelopathy of Potted Soybean Seedlings

In this study, several general y reported exogenous phenolic acids were selected as regents for potting experiments, aiming at revealing the effects of differ-ent concentrations of exogenous phenolic acids on the growth and development of soybean seedlings. The growth and development indicators of soybean seedlings treated with five different concentrations of exogenous phenolic acids, such as plant height, leaf area, root nodule number and root-shoot ratio, were determined and compared. According to the experimental results, three exogenous phenolic acids with different concentrations exhibited slight effects on plant height of potted soy-bean seedlings. The leaf area of potted soybean seedlings treated with 1.0 g/kg benzoic acid and cinnamic acid varied extremely significantly compared with the control. Root nodule number of potted soybean seedlings treated with 1.0 g/kg ben-zoic acid and vanil ic acid increased remarkably, but 1.0 g/kg cinnamic acid had lit-tle effects on root nodule number. When the concentration of phenolic acids was 1.0 g/kg, three phenolic acids exhibited the maximum effects on root-shoot ratio of potted soybean seedlings. Overal , low concentrations of exogenous phenolic acids exhibited no significant effects on the growth and development of potted soybean seedlings; high concentrations of exogenous phenolic acids inhibited the growth and development of potted soybean shoots, increased root nodule number and improved root-shoot ratio.

Research Progress on the Genetic Diversity of Wild Soybean in China

Wild soybean is recognized as the relative wild species of cultivate soy- bean. China is rich in wild soybean resources. In the past 30 years, domestic scholars have carried out a large quantity of studies on the genetic diversity of wild soybean in China, which have laid the theoretical foundation for the protection and utilization of wild soybean resources. In this paper, the research progress on the genetic diversity of wild soybean in China at the phenotypic, biochemical and molecular levels of wild soybean was reviewed, and the future development of the research on the genetic diversity of wild soybean was introduced.

Wild Soybean Germplasm Resources Conservation and Use in Shandong Province

Glycine soja or wild soybean is the wild ancestor of the soybean, being an important source for property improvement and variety breeding. With abundant wild soybeans, their habitat is undermining, and wild soybeans are facing increasing- ly threatened in Shandong if measures are not taken yet. Besides, wild soybean germplasm is far from further exploiting or using. The research analyzed status quo and problems of wild soybean germplasm posed countermeasures or suggestions in crop germplasm conservation and use. resources conservation and use and pro- order to provide references for agricultural crop germplasm conservation and use.

Relation of Primitive Moisture Content and Energy Consumption on Soybean Pod Threshing

In this research, the effects of initial moisture content and needed impact energy on threshing of soybean pods were studied. An impact device was built based of pendulum mechanism. In the experiments, threshing percentage of soybean pod samples were measured at three initial moisture content levels of 16.25% and 35% w.b. and three energy levels of 173,284 and 446 mJ. The results of analysis of variance showed that moisture content and energy have significant effects on the threshing percentage. By increasing impact energy, amount of pod threshing increased. Also in all the energy levels, maximum threshing occurred at minimum moisture. Maximum threshing percentage was 83.4%, which was at 16% moisture content and 446 mJ levels, also minimum threshing percentage was 3.3% which was at 35% moisture content and 174 mJ levels. Soybean pod with higher moisture, contact between its edges of the pod was stronger than lower moisture, so more energy was needed to separate two edges at 35% moisture.

Gene flow from transgenic roundup-ready soybean to wild soybean

A study was conducted in the field of the Institute of Vegetable Crops, Jiangsu province from July 2000 to August 2003. The transgenic roundup-ready soybean was sown in the middle of the field in a circular manner for 5 circles, with the distance of 3 m, from one circle to another. Then the wild soybean was planted in plots as the rays of the circles in 8 directions (N, E, W, S, NE, NW, SE and SW), spaced every 5 m until 50 m. Each plot comprised 25 plants. In the second year, the wild soybean seeds from the first year were planted in the field together with the original wild soybean as check. Before flowering time, high concentrations of roundups (about 4-5 times of the normal dose) were sprayed on the plants and the surviving plants were identified. The leaves were taken to the lab for DNA extraction to determine the unique DNA for roundup-ready soybean (CTAB method). About 2% of the plants survived, but some leaves were yellow. One plant of wild soybean was found to have the roundup-ready gene from the original roundup-ready soybean. The other surviving wild soybeans should also had some fragments of the roundup tolerance gene. However, the DNA bands were not very clear in the PCR map.

Exploration of Antifouling Potential of the Brown Algae Laminaria ‘Sanhai’

Seaweeds are one of the largest producers of biomass in the marine environment. It has been well known that marine algae, especially brown algae was a rich source of biogeinc compounds with antifouling potential that could be ideal alternatives of tributyltin （TBT）. In this paper, antifouling potential of the brown algae Laminaria ＇sanhai＇ was explored. Firstly, the dried alga was extracted and the antialgal and antilarval activities were investigated. The EC50 and LC50 values of crude extract of Laminaria ＇sanhai＇ against diatom （Skeletonema costatum） and barnacle larval （Chthamalus challengeri） were 8.9 μg·mL -1 and 12.0 μg·mL -1 respectively. Then, guided by bioassay, the bioactive substances were isolated by liquid-liquid extraction. The antialgal and antilarval activities of isolated fraction were improved with the EC50 value of 7.4 μg· mL -1 against S. costatum and LC50 value of 9.7 μg mL 1 against C. challengeri larvae. Identification by IR, Q-TOFMS and GC-MS of the isolated bioactive substances revealed the abundance of fatty acids. These fatty acids, most with 16, 18 or 20 carbon atoms, contained myristic, hexadecanoic, oleic, linolenic, arachidonic and eicosapentaenoic acids. The results indicated that both the crude extract and the isolated bioactive substances had high antialgal and antilarval activities with no highlighted cytotoxicity which made the brown algae Laminaria ＇sanhai＇ a promising source of the environmentally friendly antifoulants.

An International Survey on State of the Art of Grain Legume Management in Gene Banks

An online survey addressed to members listed in the European Cooperative Programme for Crop Genetic Resources Networks Working Group on Graithe current problems in the management of GL germplasm, to work out the criterian Legumes and Grain Legumes （GL） germplasm managers and breeders was carded out to pinpoint and decisions involved in the implementation of regeneration procedures and to identify strategic areas where further research is required. The survey was divided into three sections： （1） germplasm collection details and current status of the regeneration needs; （2） assessment over the understanding of basic information required to carry out appropriate regeneration procedures such as the breeding systems, the pollination requirements and pollinating agents, the isolation techniques and regeneration facilities; and （3） assessment of different options, in addition to ＂ex situ＂, such as ＂in situ＂ and ＂on farm＂ conservation. Obtaining, collating and analysing different kinds of existing data on mating system of GL species, effective pollination control methods and isolation facilities by species and location is one example of a priority issue. The GL community makes a clear request for greater support for the development of well-designed methodologies of regeneration that maintain the genetic structure of populations and that the optimum regeneration strategy is most likely to be achieved through integrating pollinators with the regeneration procedures. A major concern of the GL community is the lack of empirical scientific information on the most suitable pollinator agents.

More Benefit from Less Land： A New Rice-Pea-Rice Cropping Pattern for Resource-Poor Farmers of Bangladesh

The experiments were conducted at the Pulses Research Centre, Ishurdi, Pabna, Bangladesh during the 2005-2006 and 2006-2007 crop seasons to determine the economic viability of planting legumes for both vegetable and forage purposes in the fallow period between monsoon-rice and spring-rice. The objectives were to ensure better land utilization, break up the mono cropping, improve soil health, and generate extra-income for small and resource-poor farmers of Bangladesh. Crop compositions used in the experiments were monsoon-rice （cv. BRRIdhan-32, BRRldhan-39 and BINAdhan-4） followed by pulses （grasspea, chickpea and field pea） followed by spring-rice （cv. BRRldhan-28, BRRldhan-29 and BINAdhan-6）. Based on the data from two years in a pooled analyses, it was observed that monsoon-rice variety BINAdhan-4, followed by field pea （as vegetable ＆ forage） and spring-rice variety BINAdhan-6 produced the highest yields of 5.0 t ha-1 rice grain, 3.25 t ha1 （green vegetable） ＋ 18.1 t hal （forage） legumes and 7.8 t ha~ rice grain, respectively. The cropping pattern of monsoon-rice （BINAdhan-4）-field pea （as vegetable ＋ forage）-spring-rice （BINAdhan-6） gave the highest net return of USD$1,705 hal year-~ compared to other patterns with different rice varieties and chickpea and grasspea. This is a new finding, and is being practiced by farmers of Bangladesh who have enhanced their farm income substantially. This has also generated job opportunities for rural women to pick the green vegetable of field pea.

Effect of Self-fertilization on Performance, Breeding and Germplasm Management of Four Local Faba Bean Cultivars

Faba bean is self- and cross-fertilized species. The consequences of self-fertilization are important factors determining the germplasm management in such species with levels of heterogeneity and heterozygsity. Effects of self-fertilization on floral, yield and yield components characters were evaluated by comparing two levels of selfing, produced in bee-proof cages, in open-pollination at two locations in Sudan. Selfing process results in no significant differences in yield and yield components within each cultivar. Autofertile lines with reduced partial dependence on insects for seed set could be produced. Spatial isolation should be used to maintain the genetic purity of such lines. An alternate strategy for entries multiplication should focus on increasing heterozygosity and the maintenance of cross-fertilization inside the entries to prevent contamination with foreign pollens. Our results follow the previous knowledge on faba bean genetic resources conservation and management.

PhotobleaChing Influence in Different Phases for Coumarin 307 and Acriflavine Laser Dyes

Under high-excitation irradiance conditions to induce fluorescence, the dependence of photobleaching of Coumarin 307 （C307） and acriflavine （ACF） laser dyes in liquid and solid phases have been studied. A cw LD laser source of 1 mW and 407 nm wavelength was used as an exciting source. For one hour exposure time, it was found that the solid dye samples suffer photobleaching more than the liquid dye samples. This is because in liquid solutions the dye molecules can circulate during the irradiation, while the photobleaching is a serious problem when the dye is incorporated into solid matrix and cannot circulate.

Techno-economic Appraisal of Biodiesel from Jatropha curcas： An Egyptian Case Study

Biodiesel （fatty acid methyl ester） has been identified as a non-toxic biodegradable alternative fuel that is obtained from renewable sources. Over the last decade, there has been increasing interest in producing biodiesel from conventional sources such as soybean, canola, sunflower, and coconut oils. Current efforts are directed towards the development of new non-edible resources. Among these Jatropha Curcas comes at the forefront. In Egypt, Jatropha Curcas has grown successfully using primary treated wastewater. Also, extensive R＆D efforts identified the optimum conditions for the various processing stages namely crushing, extraction, transesterification and purification. Based on the research findings, the techno-economic appraisal of biodiesel production from Jatropha Curcas is conducted. Two nominal capacities namely 8,000 and 50,000 metric tons/yr have been proposed. Several scenarios have been formulated to take into consideration varying productivity （3.4 to 5.8 ton fruits per 4,000 m^2 （acre）） and varying recovery rates of oil from seeds. Economic indicators including capital and production costs for the various processing stages and revenues according to current prices of oil and cake have been obtained. The price of biodiesel that provides a simple rate of return （SRR） on investments of 10% was in the range of $0.3-0.7/liter for the different assumed scenarios which is lower than the prevailing price of biodiesel （about $1/liter） in the US. Thus, in view of experimental results and economic assumptions, there are positive prospects for the production of biodiesel from Jatropha Curcas under Egyptian conditions.

Response of Germination Physiology of Cajanus cajan Seeds to Drought Stress: Comparison between Karst Water and Allogenic Water Treatments

In this paper, responses of germination physiology of pigeon pea （Cajanus cajan） seeds to drought stress in karst water environment and non-karst （allogenic） water environment were studied to explore the adaptability of pigeon pea to karst environment. The results showed that： （i） Under drought stress of 20% PEG- 6000, the germination rate, vigor index, germination index and biomass of pigeon pea seeds on day 7 cultivated with karst water were all greater than that of the allogenic water treatment group, while the seed germination stress index was significantly smaller than that of the allogenic water treatment group, suggesting that karst water environment was more favorable to pigeon pea seed germination. （ii） Without drought stress, the malondialdehyde （MDA） and superoxide dismutase （SOD） activities of pigeon pea seeds cultivated with karst water were all smaller than that of the allogenic water group. However, under drought stress, the SOD activity was significantly higher than that of allogenic water group, suggesting pigeon pea SOD in karst water was able to more rapidly respond to external drought stress, and increase its own activity to reduce the damage to the plants. And （iii） with and without drought stress, the soluble protein level of the karst water group was higher than that of the allogenic water group, while the free amino acid level was lower than that of the allogenic water group. This difference was more significant with the presence of drought stress, suggesting that the karst water environment was more favorable to the accumulation of soluble proteins and thus produced larger biomass. Hence, pigeon pea is a tree species that is adapted to high-calcium, alkaline environments in karst areas, and is of great significance for the revegetation and rocky desertification control in mountainous karst areas.

Initial exploration of the mechanism underlying H2O2-induced root horizontal bending in pea

It was reported that exogenous hydrogen peroxide （H2O2） can induce primary root bend in Arabidopsis and pea. However, the mechanism remains unclear. Here we explored the mechanism underlying this phenomenon by using the pea （Pisum sativum L.） variety ＂longwan No. 1＂ The results showed that the endogenous indole-3-acetic acid （IAA） content decreased and gibberellin A3 （GA3） content increased in the curving primary pea root induced by H2O2. Meanwhile, both of the two hormones asymmetrically distributed in the inside and outside parts of the curving root. Also, the starch content decreased due to the increased a-amylase activity in this process. However, exogenous Ca2＋ can relieve the horizontal bending of pea root induced by H2O2 and altered the contents of endogenous IAA and GA3. A working model was proposed： Exogenous H2O2 causes the increase in GA3 content, and GA3 stimulates the activity of or-amylase, which leads to the hydrolysis of starch, and then the root lost the gravity perceiving. The asymmetric distribution of IAA and GA3 in two sides of curving root may cause the horizontal bending.Exogenous Ca^2＋ can relieve root bending through altering the endogenous IAA and GA3 contents.