The rice water weevil, Lissorhoptrus oryzophilus Kuschel, has two generations in southern Zhejiang, China. To determine oogenesis in first-generation females （summer females） and its relations to temperature, female...The rice water weevil, Lissorhoptrus oryzophilus Kuschel, has two generations in southern Zhejiang, China. To determine oogenesis in first-generation females （summer females） and its relations to temperature, females were collected from a rice field in early and mid-July and reared on young rice plants at 28, 31 and 34 ℃ in the laboratory. Percentage of females having oocytes, number of oocytes of different stages （stage-Ⅰ, from early previtellogenesis to middle vitellogenesis; stage-Ⅱ, late vitellogenesis; and matute-oocyte stage）, and length of ovarioles were determined every 10 d of feeding. At each temperature, oogenesis took place in over 40% of females after 20-40 d of feeding, but only 0.0-3.3 stage-Ⅰ, 0.0-0.8 stage-Ⅱ and 0.0-1.1 mature oocytes were observed at each observation date. Temperature had significant effect on number of stage-Ⅰ oocytes but not on number of stage-Ⅱ and mature oocytes in early July females; temperature had no significant effect on number of oocytes of either stage in mid-July females. Conclusively, in southern Zhejiang, summer L. oryzophilus females have great potential to become reproductive on rice, but their oogenesis activity is very low, with the overall procedures little affected by temperature.展开更多
Phylogenetic relationships among 146 species of Coleoptera （Families： Curculionidae, Staphylinidae and Carabidae） were estimated based upon mitochondrial Cytochrome Oxidase 1 gene sequences. The monophyletic of the...Phylogenetic relationships among 146 species of Coleoptera （Families： Curculionidae, Staphylinidae and Carabidae） were estimated based upon mitochondrial Cytochrome Oxidase 1 gene sequences. The monophyletic of the polyphaga and Adephaga was not supported in our study using COlgene sequences, as family Carabidae （Adephaga） was grouped with family Staphylidae （Polyphaga） with Staphylinidae paraphyletic. The subfamily Scolytinae is the most common ancestor for the subfamilies： Ceutorhynchinae, Curculioninae and Dryophtborinae and hence the oldest. The subfamily Cryptorhynchinae is the oldest among the five tested Curculionidae families. At the family level, the genetic distances and phylogenetic analysis obtained in this study showed that the family Carabidae was more related to family Staphylinidae than to family Curculionidae with the topology Staphylinida-Carabidae-Curculionidae. The topology was the same when Micromus igorotus from order Neuroptera was used as an outgroup taxon as it was Staphylinida, Carabidae, Curculionidae/Neuroptera. An alternative topology was obtained when Acytolepis puspa from order Lepidoptera was used as an outgroup that was Carabidae, Staphylinida, Curculionidae-Neuroptera/Lepidoptera. where the species of order Neuroptera placed within family Curculionida. According to the estimated genetic distances and to the standard mitochondrial DNA clock estimated at 2.3% MYA, family Curculionidae separated from family Staphylinidae and Carabidae approximately 112 and 115 MYA, respectively.展开更多
The red palm weevil Rhynchophorusferrugineus is the most important insect pest for the date palm trees in the Middle East and Gulf states. Zinc sulfate （ZnSOa＇H20） was toxicologically, biologically and physiologica...The red palm weevil Rhynchophorusferrugineus is the most important insect pest for the date palm trees in the Middle East and Gulf states. Zinc sulfate （ZnSOa＇H20） was toxicologically, biologically and physiologically evaluated as insect development disruptors against the RPW. LCs0 of Zinc sulfate against 10 days old larvae was 0.566%, 3% concentration inhibited the larvae from feeding. LCs0 concentration of zinc sulfate solution was capable to disrupting growth, development and reproduction of R. ferrugineus. Growth and development disruptions are resulted from larval feeding on the treated diet that causing percentage of mortality rate, retardation in larval and pupal duration, percentage of pupation, adults emergence, reduction in deposited eggs that laid by the resulting adults and in the egg fertility. The study further reveals that Zinc sulfate effects on histological structure of epithelial cells of the larval midgut including separation and elongation of their cells, rupture in the peritrophic membrane and in some microvilli and some degeneration of the surrounding muscles. Histological changes also was observed on the adults ovary, in this way, disrupt female gamete production through their action on the accumulation of yolk granules （vitellogenesis） and follicular epithelial cells. Disruption of male gamete production was detected by disorganized of testicular cysts and depopulation of these cysts in addition to degeneration in germ cells. Zinc sulfate may be used among other control methods by injection into the tree trunk or as fertilizer around the tree roots which in turn may have an indirect effect for controlling the RPW.展开更多
The Red Palm Weevil （RPW）, Rhynchophorus ferrugineus （Olivier）, （Coleoptera, Curculionidae, Rhynchophorinae）, is an invasive species that is originated from Southeast Asia. It has.invaded Middle East and several...The Red Palm Weevil （RPW）, Rhynchophorus ferrugineus （Olivier）, （Coleoptera, Curculionidae, Rhynchophorinae）, is an invasive species that is originated from Southeast Asia. It has.invaded Middle East and several countries of the Mediterranean Basin during the last three decades where it attacks palm trees. During the last three decades, multiple introductions of RPW to the Middle East, Europe and Caribbean （Island of Curacao, Netherland Antilles）, Lebanon and United States of America （Laguna Beach, Orange County, California） （USA） have occurred and the RPW is now a serious pest of many palm species. Duration of all life parameters varies significantly where the entire life cycle takes about 45 to 298 days. Different artificial diets were developed and were tested for mass rearing of RPW. Control of RPW is difficult due to the concealed nature of the life cycle of the pest. Integrated Pest Management （IPM） strategy has been applied successfully to deal with RPW problem. The IPM strategy consists of various preventive and curative methods; those methods were categorized in nine categories in this review. The main objective of this work was to review the existing knowledge on RPW＇s different aspects, with an ultimate aim of revealing the actual situation of the research on RPW.展开更多
The aim of the research was to develop a catch-and-release trap for monitoring and controlling RPW （Rhynchophorus ferrugineus, red palm weevil）. The RPW insects were attracted through the use of an aggregation phero...The aim of the research was to develop a catch-and-release trap for monitoring and controlling RPW （Rhynchophorus ferrugineus, red palm weevil）. The RPW insects were attracted through the use of an aggregation pheromone located into a dispenser inside the trap. The RPW adults entered through an entrance door into a sterilization room where they were sprayed with a solution containing the chemical sterilizer product ＂lufenuron＂. The adults were then forced to move into a contamination room where it was present a wet media containing spores and hyphae of Metarhizium anisopliae and spores and crystalline insecticidal proteins of Bacillus thuringensis. The sterilized and infected RPW adults were counted and released into the environment where they reached new palms and join natural populations of RPW. Preliminary experiments showed that the catch-and-release trap was able to reduce the fertility of RPW adults and to increase their mortality after contamination with entomopathogens. Moreover, the infected RPW adults were able to infect untreated RPW larvae leading to a larvae mortality of 93% within 30 days. In conclusion, the advanced catch-and-release trap represents a safe and effective tool to monitor and control the population of RPW pest without environmental pollution and risks for human health.展开更多
It was isolated eleven species of weevils family （Curculionidae） back to Subfamiles are Brachyderinae, Cleoninae, depending on the exact Microsculpture of the pits on elytra of these types, namely, Bothynoderes memo...It was isolated eleven species of weevils family （Curculionidae） back to Subfamiles are Brachyderinae, Cleoninae, depending on the exact Microsculpture of the pits on elytra of these types, namely, Bothynoderes memosae, Tanymecus sp., Cleonus orratus, Larinus maculosus, Lixus sp., Hypera sp., Amnoeleonus hiroglyphicus, Bangasternus planifrons, Phacephorus nubeculosus, Coniocleonus puseudooldiquus, Tanymecus insipidus.展开更多
基金Project supported by the National Natural Science Foundation of China (No. 30400290) and the National Basic Research Program(973) of China (No. 2002CB111403)
文摘The rice water weevil, Lissorhoptrus oryzophilus Kuschel, has two generations in southern Zhejiang, China. To determine oogenesis in first-generation females （summer females） and its relations to temperature, females were collected from a rice field in early and mid-July and reared on young rice plants at 28, 31 and 34 ℃ in the laboratory. Percentage of females having oocytes, number of oocytes of different stages （stage-Ⅰ, from early previtellogenesis to middle vitellogenesis; stage-Ⅱ, late vitellogenesis; and matute-oocyte stage）, and length of ovarioles were determined every 10 d of feeding. At each temperature, oogenesis took place in over 40% of females after 20-40 d of feeding, but only 0.0-3.3 stage-Ⅰ, 0.0-0.8 stage-Ⅱ and 0.0-1.1 mature oocytes were observed at each observation date. Temperature had significant effect on number of stage-Ⅰ oocytes but not on number of stage-Ⅱ and mature oocytes in early July females; temperature had no significant effect on number of oocytes of either stage in mid-July females. Conclusively, in southern Zhejiang, summer L. oryzophilus females have great potential to become reproductive on rice, but their oogenesis activity is very low, with the overall procedures little affected by temperature.
文摘Phylogenetic relationships among 146 species of Coleoptera （Families： Curculionidae, Staphylinidae and Carabidae） were estimated based upon mitochondrial Cytochrome Oxidase 1 gene sequences. The monophyletic of the polyphaga and Adephaga was not supported in our study using COlgene sequences, as family Carabidae （Adephaga） was grouped with family Staphylidae （Polyphaga） with Staphylinidae paraphyletic. The subfamily Scolytinae is the most common ancestor for the subfamilies： Ceutorhynchinae, Curculioninae and Dryophtborinae and hence the oldest. The subfamily Cryptorhynchinae is the oldest among the five tested Curculionidae families. At the family level, the genetic distances and phylogenetic analysis obtained in this study showed that the family Carabidae was more related to family Staphylinidae than to family Curculionidae with the topology Staphylinida-Carabidae-Curculionidae. The topology was the same when Micromus igorotus from order Neuroptera was used as an outgroup taxon as it was Staphylinida, Carabidae, Curculionidae/Neuroptera. An alternative topology was obtained when Acytolepis puspa from order Lepidoptera was used as an outgroup that was Carabidae, Staphylinida, Curculionidae-Neuroptera/Lepidoptera. where the species of order Neuroptera placed within family Curculionida. According to the estimated genetic distances and to the standard mitochondrial DNA clock estimated at 2.3% MYA, family Curculionidae separated from family Staphylinidae and Carabidae approximately 112 and 115 MYA, respectively.
文摘The red palm weevil Rhynchophorusferrugineus is the most important insect pest for the date palm trees in the Middle East and Gulf states. Zinc sulfate （ZnSOa＇H20） was toxicologically, biologically and physiologically evaluated as insect development disruptors against the RPW. LCs0 of Zinc sulfate against 10 days old larvae was 0.566%, 3% concentration inhibited the larvae from feeding. LCs0 concentration of zinc sulfate solution was capable to disrupting growth, development and reproduction of R. ferrugineus. Growth and development disruptions are resulted from larval feeding on the treated diet that causing percentage of mortality rate, retardation in larval and pupal duration, percentage of pupation, adults emergence, reduction in deposited eggs that laid by the resulting adults and in the egg fertility. The study further reveals that Zinc sulfate effects on histological structure of epithelial cells of the larval midgut including separation and elongation of their cells, rupture in the peritrophic membrane and in some microvilli and some degeneration of the surrounding muscles. Histological changes also was observed on the adults ovary, in this way, disrupt female gamete production through their action on the accumulation of yolk granules （vitellogenesis） and follicular epithelial cells. Disruption of male gamete production was detected by disorganized of testicular cysts and depopulation of these cysts in addition to degeneration in germ cells. Zinc sulfate may be used among other control methods by injection into the tree trunk or as fertilizer around the tree roots which in turn may have an indirect effect for controlling the RPW.
文摘The Red Palm Weevil （RPW）, Rhynchophorus ferrugineus （Olivier）, （Coleoptera, Curculionidae, Rhynchophorinae）, is an invasive species that is originated from Southeast Asia. It has.invaded Middle East and several countries of the Mediterranean Basin during the last three decades where it attacks palm trees. During the last three decades, multiple introductions of RPW to the Middle East, Europe and Caribbean （Island of Curacao, Netherland Antilles）, Lebanon and United States of America （Laguna Beach, Orange County, California） （USA） have occurred and the RPW is now a serious pest of many palm species. Duration of all life parameters varies significantly where the entire life cycle takes about 45 to 298 days. Different artificial diets were developed and were tested for mass rearing of RPW. Control of RPW is difficult due to the concealed nature of the life cycle of the pest. Integrated Pest Management （IPM） strategy has been applied successfully to deal with RPW problem. The IPM strategy consists of various preventive and curative methods; those methods were categorized in nine categories in this review. The main objective of this work was to review the existing knowledge on RPW＇s different aspects, with an ultimate aim of revealing the actual situation of the research on RPW.
文摘The aim of the research was to develop a catch-and-release trap for monitoring and controlling RPW （Rhynchophorus ferrugineus, red palm weevil）. The RPW insects were attracted through the use of an aggregation pheromone located into a dispenser inside the trap. The RPW adults entered through an entrance door into a sterilization room where they were sprayed with a solution containing the chemical sterilizer product ＂lufenuron＂. The adults were then forced to move into a contamination room where it was present a wet media containing spores and hyphae of Metarhizium anisopliae and spores and crystalline insecticidal proteins of Bacillus thuringensis. The sterilized and infected RPW adults were counted and released into the environment where they reached new palms and join natural populations of RPW. Preliminary experiments showed that the catch-and-release trap was able to reduce the fertility of RPW adults and to increase their mortality after contamination with entomopathogens. Moreover, the infected RPW adults were able to infect untreated RPW larvae leading to a larvae mortality of 93% within 30 days. In conclusion, the advanced catch-and-release trap represents a safe and effective tool to monitor and control the population of RPW pest without environmental pollution and risks for human health.
文摘It was isolated eleven species of weevils family （Curculionidae） back to Subfamiles are Brachyderinae, Cleoninae, depending on the exact Microsculpture of the pits on elytra of these types, namely, Bothynoderes memosae, Tanymecus sp., Cleonus orratus, Larinus maculosus, Lixus sp., Hypera sp., Amnoeleonus hiroglyphicus, Bangasternus planifrons, Phacephorus nubeculosus, Coniocleonus puseudooldiquus, Tanymecus insipidus.
