[Objective] The aim was to study the construction and expression of eukaryotic expression vectors of antibacterial peptides （mytilin and myticin） from Mytilus coruscus.[Method] By the screening of antibacterial pept...[Objective] The aim was to study the construction and expression of eukaryotic expression vectors of antibacterial peptides （mytilin and myticin） from Mytilus coruscus.[Method] By the screening of antibacterial peptide genes of mytilin and myticin of Mytilus coruscus,five antibacterial peptide genes were selected.Then,the relative eukaryotic expressing vectors were constructed by the use of PCR technique and DNA recombinant technology.Subsequently,they were transferred in to S78 Saccharomyces cerevisia by using LiAC transformation method,and then preliminary expressing analysis was carried out.[Result] Five eukaryotic expressing vectors of antibacterial peptides from Mytilus coruscus were successfully constructed,and the results of mRNA detection revealed that the five antibacterial peptides from Mytilus coruscus were successfully transcribed.[Conclusion] The results provide a basis for using genetic engineering to express antibacterial peptides of mytilin and myticin from Mytilus coruscus,and for developing the further study of antibacterial peptides from Mytilus coruscus based on this.展开更多
基金Supported by Agricultural Research Project of Science and Technology Department of Zhejiang Province(2008C22026,2009C32016)Open Topic of Key Open Laboratory of Marine and Estuarine Fishery Resources and Ecology, Ministry of Agriculture ( Open-09-10 )+1 种基金Technology Bureau Project of Zhoushan City (Y20082080)Innovative Business Incubation Program of College Students~~
文摘[Objective] The aim was to study the construction and expression of eukaryotic expression vectors of antibacterial peptides （mytilin and myticin） from Mytilus coruscus.[Method] By the screening of antibacterial peptide genes of mytilin and myticin of Mytilus coruscus,five antibacterial peptide genes were selected.Then,the relative eukaryotic expressing vectors were constructed by the use of PCR technique and DNA recombinant technology.Subsequently,they were transferred in to S78 Saccharomyces cerevisia by using LiAC transformation method,and then preliminary expressing analysis was carried out.[Result] Five eukaryotic expressing vectors of antibacterial peptides from Mytilus coruscus were successfully constructed,and the results of mRNA detection revealed that the five antibacterial peptides from Mytilus coruscus were successfully transcribed.[Conclusion] The results provide a basis for using genetic engineering to express antibacterial peptides of mytilin and myticin from Mytilus coruscus,and for developing the further study of antibacterial peptides from Mytilus coruscus based on this.
