Background/Aims: Liver negative-strand hepatitis C virus (HCV) RNA is the most direct indicator of active viral replication but has only been examined in a few semiquantitative studies. Methods: Positive-and negative-...Background/Aims: Liver negative-strand hepatitis C virus (HCV) RNA is the most direct indicator of active viral replication but has only been examined in a few semiquantitative studies. Methods: Positive-and negative-strand HCV RNA in the right (R) and left (L) liver lobes was quantified by rTth-based strand-specific real-time polymerase chain reaction for 48 chronic hepatitis C patients. Results: Close correlations between lobes were seen for positive-and negative-strand amounts (r=0.950; P < 0.001 and r=0.920; P < 0.001, respectively). The ratio of negative to positive strands (median, 0.14 for R and 0.13 for L) varied by 2 log directly in relation to HCV replication assessed by liver negative strands but had no relation to liver positive strands and circulating HCV. Only negative-strand quantitation was inversely correlated with age (r=-0.322; P=0.026 for R and r=-0.340; P=0.018 for L), while liver tissues with hepatitis B virus DNA contained larger amounts of each strand. In 27 patients treated with enhanced interferon monotherapy, the amounts of liver negative strands (< 4 log copies/100 ng RNA) were the only independent predictor of a sustained virologic response. Conclusions: Negative-strand quantitation is uniform in the liver and bears distinct relevance to the disease.展开更多
疫苗免疫是防控人类和动物疫病最经济有效的策略。以适宜的病毒、细菌等微生物或寄生虫为载体的活载体疫苗具有安全性高、能激发宿主多类型免疫应答、不需要佐剂等优势,是目前疫苗研究领域的热点之一。其中,以病毒为疫苗载体的研究最为...疫苗免疫是防控人类和动物疫病最经济有效的策略。以适宜的病毒、细菌等微生物或寄生虫为载体的活载体疫苗具有安全性高、能激发宿主多类型免疫应答、不需要佐剂等优势,是目前疫苗研究领域的热点之一。其中,以病毒为疫苗载体的研究最为广泛,技术也最为成熟,并且已有许多商品化的重组病毒载体疫苗。副流感病毒5型(Parainfluenza virus type 5,PIV5)为单负链RNA病毒,感染宿主范围广,单独感染一般不引起临床症状,致病性低。展开更多
文摘Background/Aims: Liver negative-strand hepatitis C virus (HCV) RNA is the most direct indicator of active viral replication but has only been examined in a few semiquantitative studies. Methods: Positive-and negative-strand HCV RNA in the right (R) and left (L) liver lobes was quantified by rTth-based strand-specific real-time polymerase chain reaction for 48 chronic hepatitis C patients. Results: Close correlations between lobes were seen for positive-and negative-strand amounts (r=0.950; P < 0.001 and r=0.920; P < 0.001, respectively). The ratio of negative to positive strands (median, 0.14 for R and 0.13 for L) varied by 2 log directly in relation to HCV replication assessed by liver negative strands but had no relation to liver positive strands and circulating HCV. Only negative-strand quantitation was inversely correlated with age (r=-0.322; P=0.026 for R and r=-0.340; P=0.018 for L), while liver tissues with hepatitis B virus DNA contained larger amounts of each strand. In 27 patients treated with enhanced interferon monotherapy, the amounts of liver negative strands (< 4 log copies/100 ng RNA) were the only independent predictor of a sustained virologic response. Conclusions: Negative-strand quantitation is uniform in the liver and bears distinct relevance to the disease.
文摘疫苗免疫是防控人类和动物疫病最经济有效的策略。以适宜的病毒、细菌等微生物或寄生虫为载体的活载体疫苗具有安全性高、能激发宿主多类型免疫应答、不需要佐剂等优势,是目前疫苗研究领域的热点之一。其中,以病毒为疫苗载体的研究最为广泛,技术也最为成熟,并且已有许多商品化的重组病毒载体疫苗。副流感病毒5型(Parainfluenza virus type 5,PIV5)为单负链RNA病毒,感染宿主范围广,单独感染一般不引起临床症状,致病性低。
