目的对一起养殖泥鳅病害进行发病情况、临床表现与病理变化、病原等方面的检验,旨在明确感染症的特征及相应的病原菌。方法随机取6尾病死泥鳅的肝及溃烂组织做细菌分离,对分离菌进行形态特征、生长表现、理化特性及代表菌株16S rRNA基...目的对一起养殖泥鳅病害进行发病情况、临床表现与病理变化、病原等方面的检验,旨在明确感染症的特征及相应的病原菌。方法随机取6尾病死泥鳅的肝及溃烂组织做细菌分离,对分离菌进行形态特征、生长表现、理化特性及代表菌株16S rRNA基因序列测定等方面的鉴定明确细菌种类。选择代表菌株制备成3×108CFU/ml的菌液,分别经腹腔、肌肉途径感染健康泥鳅,测定其致病作用;对鉴定后的菌株以琼脂扩散法(K-B)做对抗菌类药物的敏感性测定。结果被检病死泥鳅的下颌部肌肉溃烂,肝脏及脾脏肿胀等。从肝、溃烂组织均检出了纯一的同种细菌,经鉴定表明为气单胞菌属(Aero-monasKluyver and van Niel1936)的新种并定名为泥鳅气单胞菌(Aeromonals misgurnussp.nov.)。人工感染试验结果对泥鳅具有较强的致病作用,用37种抗菌类药物所做的药敏试验结果显示在不同株间无明显的敏感与耐药性差异。结论所检病例为细菌性败血感染症,其病原为泥鳅气单胞菌。展开更多
The concepts of systemic inflammatory response syndrome （SIRS） and scoring system were defined by the journal of Bone in 1992. SIRS was described as occurrence of two or more clinical criteria in four ones （fever o...The concepts of systemic inflammatory response syndrome （SIRS） and scoring system were defined by the journal of Bone in 1992. SIRS was described as occurrence of two or more clinical criteria in four ones （fever or hypothermia, tachypnea, tachycardia, and leukocytosis）. An early diagnosis and estimation of systemic inflammation in patients is helpful for treatment selection. This paper reviews the application of SIRS scoring system, which has been extensively validated for large groups of critical carepatients with severe injury and critical surgical diseases. Recent studies have documented SIRS score as a significant predictive parameter of adverse outcome in critical care patients. Furthermore, some studies also give us a suggestion on how to reduce the overload systemic response.展开更多
Objective： Measurement of biomarkers is a potential approach to early prediction of the risk of mortality in patients with sepsis. The aim of the present study was to evaluate the prognostic value of pro-atrial natri...Objective： Measurement of biomarkers is a potential approach to early prediction of the risk of mortality in patients with sepsis. The aim of the present study was to evaluate the prognostic value of pro-atrial natriuretic peptide （pro-ANP） and pro-adrenomedullin （pro- ADM） levels in a cohort of medical intensive care patients and to compare it with that of other known biomarkers and physiological scores. Methods： Blood samples of 51 consecutive critically ill patients admitted to the intensive care unit and 53 age-matched healthy control people were evaluated in this prospective study. The prognostic value ofpro-ANP and pro-ADM levels was compared with that of acute physiology and chronic health evaluation （APACHE） II scores and various biomarkers such as C-reactive protein, interleukin-6 and procalcitonin. Pro-ANP and pro-ADM were detected by a new sandwich immunoassay. Results： On admission, 25 patients had systemic inflammatory response syndrome （SIRS）, 12 sepsis, 9 severe sepsis and 5 septic shock. At that time, the median levels （ng/ml） of pro-ANP and pro-ADM were 87.22 and 0.34 respectively in patients with SIRS, 1533.30 and 2.23 in those with sepsis, 1098.73 and 4.57 in those with severe sepsis, and 1933.94 and 8.21 in those with septic shock. With the increasing severity of disease, the levels of pro- ANP and pro-ADM were gradually increased. On admission, the circulating levels ofpro-ANP and pro-ADM in patients with sepsis, severe sepsis, or septic shock were significantly higher in non-survivors than in survivors （P〈0.05）. In a receiver operating characteristic curve analysis for the survival of patients with sepsis, the areas under the curve （AUCs） for pro-ANP and pro-ADM were 0.89 and 0.87 respectively, which was similar to the AUCs for procalcitonin and APACHE II scores. Conclusion： Pro-ANP and pro-ADM are valuable biomarkers for prediction of severity of septic patients.展开更多
文摘目的对一起养殖泥鳅病害进行发病情况、临床表现与病理变化、病原等方面的检验,旨在明确感染症的特征及相应的病原菌。方法随机取6尾病死泥鳅的肝及溃烂组织做细菌分离,对分离菌进行形态特征、生长表现、理化特性及代表菌株16S rRNA基因序列测定等方面的鉴定明确细菌种类。选择代表菌株制备成3×108CFU/ml的菌液,分别经腹腔、肌肉途径感染健康泥鳅,测定其致病作用;对鉴定后的菌株以琼脂扩散法(K-B)做对抗菌类药物的敏感性测定。结果被检病死泥鳅的下颌部肌肉溃烂,肝脏及脾脏肿胀等。从肝、溃烂组织均检出了纯一的同种细菌,经鉴定表明为气单胞菌属(Aero-monasKluyver and van Niel1936)的新种并定名为泥鳅气单胞菌(Aeromonals misgurnussp.nov.)。人工感染试验结果对泥鳅具有较强的致病作用,用37种抗菌类药物所做的药敏试验结果显示在不同株间无明显的敏感与耐药性差异。结论所检病例为细菌性败血感染症,其病原为泥鳅气单胞菌。
基金This study was supported by the National Key Basic Research and Project Foundation of China ( No. 2005CB522602) the Military Medicine Research Foundation of Third Military Medical University ( No. XJYY200314).
文摘The concepts of systemic inflammatory response syndrome （SIRS） and scoring system were defined by the journal of Bone in 1992. SIRS was described as occurrence of two or more clinical criteria in four ones （fever or hypothermia, tachypnea, tachycardia, and leukocytosis）. An early diagnosis and estimation of systemic inflammation in patients is helpful for treatment selection. This paper reviews the application of SIRS scoring system, which has been extensively validated for large groups of critical carepatients with severe injury and critical surgical diseases. Recent studies have documented SIRS score as a significant predictive parameter of adverse outcome in critical care patients. Furthermore, some studies also give us a suggestion on how to reduce the overload systemic response.
文摘Objective： Measurement of biomarkers is a potential approach to early prediction of the risk of mortality in patients with sepsis. The aim of the present study was to evaluate the prognostic value of pro-atrial natriuretic peptide （pro-ANP） and pro-adrenomedullin （pro- ADM） levels in a cohort of medical intensive care patients and to compare it with that of other known biomarkers and physiological scores. Methods： Blood samples of 51 consecutive critically ill patients admitted to the intensive care unit and 53 age-matched healthy control people were evaluated in this prospective study. The prognostic value ofpro-ANP and pro-ADM levels was compared with that of acute physiology and chronic health evaluation （APACHE） II scores and various biomarkers such as C-reactive protein, interleukin-6 and procalcitonin. Pro-ANP and pro-ADM were detected by a new sandwich immunoassay. Results： On admission, 25 patients had systemic inflammatory response syndrome （SIRS）, 12 sepsis, 9 severe sepsis and 5 septic shock. At that time, the median levels （ng/ml） of pro-ANP and pro-ADM were 87.22 and 0.34 respectively in patients with SIRS, 1533.30 and 2.23 in those with sepsis, 1098.73 and 4.57 in those with severe sepsis, and 1933.94 and 8.21 in those with septic shock. With the increasing severity of disease, the levels of pro- ANP and pro-ADM were gradually increased. On admission, the circulating levels ofpro-ANP and pro-ADM in patients with sepsis, severe sepsis, or septic shock were significantly higher in non-survivors than in survivors （P〈0.05）. In a receiver operating characteristic curve analysis for the survival of patients with sepsis, the areas under the curve （AUCs） for pro-ANP and pro-ADM were 0.89 and 0.87 respectively, which was similar to the AUCs for procalcitonin and APACHE II scores. Conclusion： Pro-ANP and pro-ADM are valuable biomarkers for prediction of severity of septic patients.
