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烟草ftsZ基因表达对质体发生的影响 被引量:3
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作者 董淑杰 王东 +3 位作者 孔冬冬 何奕昆 孙敬三 刘永胜 《自然科学进展》 北大核心 2002年第3期303-306,共4页
FtsZ是广泛存在于原核生物和高等植物中的一种功能蛋白,它控制着原核细胞和质体的分裂过程.为进一步认识原核细胞和质体分裂的分子机制及真核细胞的起源和进化等重要问题,研究了该基因的表达调控特征.从烟草克隆ftsZ cDNA,构建了谷胱甘... FtsZ是广泛存在于原核生物和高等植物中的一种功能蛋白,它控制着原核细胞和质体的分裂过程.为进一步认识原核细胞和质体分裂的分子机制及真核细胞的起源和进化等重要问题,研究了该基因的表达调控特征.从烟草克隆ftsZ cDNA,构建了谷胱甘肽转移酶(GST)与FtsZ融合蛋白的表达质粒,并将其导入到在异丙基-β-D-硫代半乳糖苷(IPTG)诱导下能高效表达的大肠杆菌JM109中.高表达的融合蛋白通过谷胱甘肽-琼脂糖亲和层析和SDS-聚丙烯酰胺凝胶电泳纯化后,用以免疫兔子制备抗血清.免疫印迹法表明烟草frsZ基因的表达具有明显的组织器官特征,在质体(叶绿体)分裂活跃的部位表达强,其表达量为幼嫩花瓣>幼叶>幼根>老叶>茎.黑暗培养1d对ftsZ基因表达似乎无影响,随黑暗培养时间延长,FtsZ蛋白表达逐渐降低,叶绿体转化成为体积小、数目众多(增加2~3倍)的淡黄色或白色质体.该实验结果证明,光对植物ftsZ基因表达很可能无直接影响,ftsZ基因表达的强弱是决定质体(叶绿体)分裂和细胞中质体(叶绿体)数目多少的主要原因之一. 展开更多
关键词 基因表达 质体发生 烟草 FtsZ基因 免疫印迹法 质体分裂 叶绿体 分子机制 质体起源
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Studies on Parameters Affecting Embryogenesis of Protoplasts Isolated from Suspension of Embryogenic Cells in Loblolly Pine 被引量:1
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作者 唐巍 欧阳藩 郭仲琛 《Developmental and Reproductive Biology》 1997年第2期59-66,共8页
Protoplasts of embryogenic suspension cells of loblolly pine (Pinus taeda L).were isolated at exponential growth stage.Influences of various concentrations of basal medium,levels of BA,and concentrations of inositol ... Protoplasts of embryogenic suspension cells of loblolly pine (Pinus taeda L).were isolated at exponential growth stage.Influences of various concentrations of basal medium,levels of BA,and concentrations of inositol on the differentiation of embryonal suspensor mass (ESM),early stage somatic embryos (ESE) ,and lae stage somatic embryos (LSE) were investigated .A study of the effect of various concentrations of LP basal medium sowed that the optimal basal medium concentration of ESM,ESE,and LSE differentiation was 1.25 LP medium.The effects of various levels of BA and inositol showed that the optimal concentrations of BA for the formation of ESM,ESE and LSE were 4 mg/L ,2mg/L and 1mg/L,respectively ,and the optimal concentrations of inositol for the ESM ,ESE and LSM formation were 400mg/L,800mg/L and 1,200mg/L,respectively. 展开更多
关键词 Pinus taeda L. suspension cell protoplast somatic embryogenesis
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The effect of external Ca^(2+) and Ca^(2+)-channel modulators on red-light-induced swelling of protoplasts of Phaseolus radiatus L. 被引量:1
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作者 LONGCHENG XIAOJINGWANG 《Cell Research》 SCIE CAS CSCD 1998年第1期41-50,共10页
Red-light-induced swelling of the protoplasts isolated from hypocotyl of etiolated mung bean (Phaseolus radiatus L.) was observed only when Ca2+ ions were present in the medium. The optimal CaCl2 concentration was 250... Red-light-induced swelling of the protoplasts isolated from hypocotyl of etiolated mung bean (Phaseolus radiatus L.) was observed only when Ca2+ ions were present in the medium. The optimal CaCl2 concentration was 250 μM. Swelling response declined when Ca2+ was supplied into the medium after red light irradiation. The Ca2+-chelator EGTA eliminated the red-light-induced swelling and 45Ca2+ accumulation in the protoplasts. In contrast, A23187, a Ca2+-ionophore, could mimic the effect of red light in darkness. These results indicate that Ca2+ may play a role in light signal transduction. In addition, swelling response was prevented by TFP and CPZ (both are CaM antagonists), implying the involvement of CaM in red-light-induced and Ca2+ -dependent protoplast swelling. 展开更多
关键词 Calcium ions red light protoplast swelling ^(45)Ca^(2+) accumulation Phaseolus radiatus
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Early development of Grateloupia turuturu (Halymeniaceae, Rhodophyta) 被引量:4
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作者 王高歌 姜春梅 +2 位作者 王莎莎 魏晓娇 赵凤娟 《Chinese Journal of Oceanology and Limnology》 SCIE CAS CSCD 2012年第2期264-268,共5页
Grateloupia turuturu is a commercial red alga with potential value in nutraceuticals and pharmaceuticals. To supplement information on its life history and verify whether carpospores can be used for seedling culture, ... Grateloupia turuturu is a commercial red alga with potential value in nutraceuticals and pharmaceuticals. To supplement information on its life history and verify whether carpospores can be used for seedling culture, early development of G. turuturu was investigated under culture conditions (27~C, 10-13 ~mol/(mZ.s) in irradiance, photoperiod 10:14 h L:D). Three physiological stages were recognized by continuous microscopic observation: division stage, discoid crust stage, and juvenile seedling stage. At the beginning of the division stage, the carpospores developed germ tubes into which the carpospore protoplasm was evacuated, and then the carpospore protoplasm in the germ tubes began to divide continuously until discoid crusts formed. Finally, upright thalli appeared on the discoid crusts and developed into juvenile seedlings. It took about 60 days for carpospores to develop into juvenile seedlings. The growth parameters, including germination rate for carpospores and discoid crust diameter, were recorded. These results contribute more information on the life cycle, and at the same time are of great significance in the scaling-up of artificial seedling cultures of G. turuturu. 展开更多
关键词 Grateloupia turuturu early development carpospores
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The Study on Improving the Health Condition through Sports
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作者 Senlin LU Yi HUAI 《International Journal of Technology Management》 2015年第6期113-115,共3页
In the 1990s, China began to develop mass sports, it is committed to improving the health condition, and it will improve the sport as an important form of universal health. While national sports development, China' s... In the 1990s, China began to develop mass sports, it is committed to improving the health condition, and it will improve the sport as an important form of universal health. While national sports development, China' s sports begin to flourish and have achieved remarkable record. For example, in the just-concluded 2014, in the Incheon Asian Games, China has made a total score first. This shows that sports have become an important part of national development to be reckoned with. With economic development, the accelerated pace of life is by the people neglect their own physical exercise, so that declining physical fitness, health groups continues to grow. So how in the development of sports at the same time, ensure the health sciences to improve the health condition. This paper discusses this correlation. 展开更多
关键词 SPORTS FITNESS Research
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Study on the Research and Application of Network Teaching Courseware in Aerobics in Colleges and Universities
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作者 Xiangxin Meng Huanxiang Ding +1 位作者 Yongjun Zhao Xueqin Zhuang 《International English Education Research》 2014年第11期41-45,共5页
Aerobics teaching in colleges and universities is the most important part of higher education, and the important means to carry out quality-oriented education and cultivate all-round developed talents. The reform of a... Aerobics teaching in colleges and universities is the most important part of higher education, and the important means to carry out quality-oriented education and cultivate all-round developed talents. The reform of aerobics teaching in colleges and universities should be geared to the demands of the times, and explore new innovation and continual development. In this paper, using the research methods of documentation, courseware development, teaching experiment and mathematical statistics, we study on the making and application of college aerobics network teaching courseware. It is revealed that, with the combination of modem network technology and college aerobics teaching, the resulted college aerobics network teaching courseware is the important media to not only upgrades college aerobics teaching quality, but also help develop students' habits of doing physical exercise, and promote physical health. Therefore, this is meaningful and practical in promoting the education of college students in physical quality development. 展开更多
关键词 Colleges and Universities Aerobics the Network Teaching Courseware Prepare Test Application
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The Research of the Influential Factors of Wearing Comfort of Functional Underwear
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作者 LIU Weimin XIE Hong 《International English Education Research》 2016年第5期24-27,共4页
Along with the rich material life, the change of the diet structure, people's shape also began to change.With performance m females is the body fat's increasing, the shape is not beautiful, therefore, body sculpting... Along with the rich material life, the change of the diet structure, people's shape also began to change.With performance m females is the body fat's increasing, the shape is not beautiful, therefore, body sculpting underwear has become the first choice for women to pursue beauty.To the requirement of sculpting body underwear is also becoming higher and higher for women.Comfortable and fashion is the sign of measuring a sculpting body underwear quality. This paper introduces the development history of sculpting body underwear, sculpting body underwear comfort evaluation index.And from the three aspects of the human body, clothing, environment do research to sculpting body underwear about the influence factors of wearing comfort.lt provides the certain reference value for consumers to purchase the sculpting body underwear and for enterprise to product sculpting body underwear. 展开更多
关键词 sculpting body underwear influencing factors pressure comfort
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BCCIPβ modulates the ribosomal and extraribosomal function of S7 through a direct interaction
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作者 Qian Ba Xiaoguang Li +13 位作者 Chao Huang Junyang Li Yijing Fu Peizhan Chen Juan Duan Miao Hao Yinghua Zhang Jingquan Li Chuanqi Sun Hao Ying Haiyun Song Ruiwen Zhang Zhiyuan Shen Hui Wang 《Journal of Molecular Cell Biology》 SCIE CAS CSCD 2017年第3期209-219,共11页
Extraribosomal functions of ribosomal proteins (RPs) have gained much attention for their implications in tumorigenesis and pro- gression. However, the regulations for transition between the ribosomal and extraribos... Extraribosomal functions of ribosomal proteins (RPs) have gained much attention for their implications in tumorigenesis and pro- gression. However, the regulations for transition between the ribosomal and extraribosomal functions of RPs are rarely reported. Herein, we identified a ribosomal protein S7-interacting partner, BCCIPβ, which modulates the functional conversion of S7. Through the N-terminal acidic domain, BCCIPβ interacts with the central basic region in S7 and regulates the extraribosomal dis- tribution of S7. BCCIPI~ deficiency abrogates the ribosomal accumulation but enhances the ribosome-free location of S7. This translocation further impairs protein synthesis and triggers ribosomal stress. Consequently, BCCIPβ deficiency suppresses the ribosomal function and initiates the extraribosomal function of S7, resulting in restriction of cell proliferation. Moreover, clinically relevant S7 mutations were found to dampen the interaction with BCCIPβ and facilitate the functional transition of S7. In conclu- sion, BCCIPβ, as a S7 modulator, contributes to the regulation of ribosomal and extraribosomai functions of S7 and has implica- tions in cell growth and tumor development. 展开更多
关键词 BCCIPβ ribosomal protein S7 TRANSLATION ribosomal stress tumor suppressor
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