外源铁蛋白基因(Pea-Fer)对稻米品质性状的影响

利用外源豌豆铁蛋白基因(Pea-Fer)的转化系Fer34及其受体亲本粳稻(Oryza sativa ssp.japan-ica)品种秀水11(XS11),经过连续回交和自交,并结合GUS标记基因辅助选择技术,获得了含有Pea-Fer基因的Fer34-XS11。该纯系与原受体亲本秀水11构成一对近等基因系。以该对近等基因系为材料,研究外源铁蛋白基因(Pea-Fer)对稻米主要品质性状的影响。结果发现,在稻米外观和蒸煮食用品质方面,转基因品系的外型、垩白、透明度、糊化温度、胶稠度和直链淀粉含量等性状与对照基本相同;在稻米主要营养品质方面,转基因品系除稻米籽粒中铁含量比对照有显著增加以外,其余检测的矿质元素、蛋白质、氨基酸含量等都无显著性差异;在稻米胚乳淀粉及其结构方面,转基因品系的淀粉黏滞性(RVA谱)、米饭质地及其电镜下淀粉粒结构和排列等,均与对照相仿。可见,外源豌豆铁蛋白基因(Pea-Fer)的转入,主要提高了转基因品系稻米籽粒中的铁含量,但并未影响稻米的主要品质性状。

Preparation and interface state of phosphate tailing-based geopolymers

The long-term storage of phosphate tailings will occupy a large amount of land,pollute soil and groundwater,thus,it is crucial to achieve the harmless disposal of phosphate tailings.In this study,high-performance geopolymers with compressive strength of 38.8 MPa were prepared by using phosphate tailings as the main raw material,fly ash as the active silicon-aluminum material,and water glass as the alkaline activator.The solid content of phosphate tailings and fly ash was 60% and 40%,respectively,and the water-cement ratio was 0.22.The results of XRD,FTIR,SEM-EDS and XPS show that the reactivity of phosphate tailings with alkaline activator is weak,and the silicon-aluminum material can react with alkaline activator to form zeolite and gel,and encapsulate/cover the phosphate tailings to form a dense phosphate tailings-based geopolymer.During the formation of geopolymers,part of the aluminum-oxygen tetrahedron replaced the silicon-oxygen tetrahedron,causing the polycondensation reaction between geopolymers and increasing the strength of geopolymers.The leaching toxicity test results show that the geopolymer has a good solid sealing effect on heavy metal ions.The preparation of geopolymer from phosphate tailings is an important way to alleviate the storage pressure and realize the resource utilization of phosphate tailings.

Formation, distribution, and nature of Picea mongolica in China

Picea mongolica is an endemic and endangered species in China. Ecosystem made of Picea mongolica is a special sandy forest ecosystem in China. It is found at ecotone between forest and steppe, or agricultural district and pastoral area. Based on investigation, this paper discussed the formation and distribution of Picea mongolica and studied its nature according to ecotone theory. It is clarified that Picea mongolica belongs to Picea meyeri series. That is to say, it became a local race through long-term adaptation to the local climate, then formed allopatric semi-species, and finally turned into a taxonomical species. Picea mongolica forest is a super zonal climax community developing in ecotone between forest zone and steppe zone.

Analysis for Soil Characteristics of Degraded Grassland on Alpine Meadow

[Objective]The aim of this study is to explore the effects of grassland degradation on soil physical and chemical properties.[Method]The ratio of plant root to soil and soil texture on Alpine Meadow were investigated in this study,and soil available N,P,K,Cu,Zn,organic matter and pH value were also analyzed by routine analysis of soil nutrients in different degraded grasslands.[Result]With the intensification of degraded gradient and the soil depth,the ratio of plant root to soil was decreased gradually.The highest ratio of plant root to soil was in 0-10 cm depth of soil in grassland with different degraded gradients,while its ratio of plant root to soil changed from 0.001 to 0.040 with soil type of loam.Soil chemical characteristic changed in different degraded gradients.The content of available N,P,K reduced significantly with the soil depth and the intensification of degraded gradients.The content of Cu and Zn was relatively lack in degraded grassland.[Conclusion]There is no significant correlation between nutrition content or pH value and the succession degree of degraded grassland.

Genetic variations of wood properties and growth characters of Ko-rean pines from different provenances

Totally 26 provenance stands of 17-year-old Korean pine were selected for investigating wood properties and growth characters in Mao抏rshan Experimental Forest Farm of Northeast Forestry University in 1999. The anatomical property indexes, including tracheid length, tracheid diameter and wall-indiameter ratio, and the physical property indexes, such as growth ring width, late wood percentage and growth ring density, were measured for wood properties. Growth character indexes, including tree height and diameter at breast height, were also measured. The analytical results showed that there exited obviously dif-ference in wood property indexes between different provenances, which is suggested that wood properties are controlled by their genetic differences. The growth character indexes of Korean pines presented significant difference and they might also be controlled by their genetic differences. Most parameters of wood properties mainly varied in the direction of longitude, but the parameters of growth characters varied in the direction of latitude.

Highlighting display of geologic bodies based on directivity filtering

To make a geologic body more outstanding, it is necessary to preserve edges when filtering. For this reason, a filtering method based on anisotropic diffusion is introduced. The key point of this method is that diffusion filtering will be made along the edge while diffusion is forbidden in the direction perpendicular to the edge, so the filtering has directionality. When doing anisotropic diffusion filtering, parameters like diffusion coefficient and threshold have great impacts on the results, so the anisotropic diffusion model parameters are discussed, the diffusion coefficient equation is introduced, and the diffusion threshold selection criterion is derived and analyzed. In addition, this method was used with the diffusion coefficient equation＇s proper diffusion threshold to highlight the tidal channel geobodies in the XX area, sand body in the YY area, and faults in the ZZ area. The good delineation results prove that the diffusion threshold selection criterion introduced in this paper is reasonable.

The algorithm of 3D multi-scale volumetric curvature and its application

To fully extract and mine the multi-scale features of reservoirs and geologic structures in time/depth and space dimensions, a new 3D multi-scale volumetric curvature （MSVC） methodology is presented in this paper. We also propose a fast algorithm for computing 3D volumetric curvature. In comparison to conventional volumetric curvature attributes, its main improvements and key algorithms introduce multi-frequency components expansion in time-frequency domain and the corresponding multi-scale adaptive differential operator in the wavenumber domain, into the volumetric curvature calculation. This methodology can simultaneously depict seismic multi-scale features in both time and space. Additionally, we use data fusion of volumetric curvatures at various scales to take full advantage of the geologic features and anomalies extracted by curvature measurements at different scales. The 3D MSVC can highlight geologic anomalies and reduce noise at the same time. Thus, it improves the interpretation efficiency of curvature attributes analysis. The 3D MSVC is applied to both land and marine 3D seismic data. The results demonstrate that it can indicate the spatial distribution of reservoirs, detect faults and fracture zones, and identify their multi-scale properties.

Genetic Diversity of Achnatherum Splendens

[Objective] Study on the genetic diversities of A.splendens from different areas of xinjiang. [Method] The genetic diversities among seven populations and within two populations were analyzed by RAPD. [Result] Genetic clustering results presented that the relationships among populations of A. splendens are directly related with geographic positions, and within population are related with habitats. [Conclusion] RAPD technique can be used to study genetic diversity of A. splendens.

Effects of Artificial Vegetation Restoration on Soil Physicochemical Properties in Southern Edge of Mu Us Sandy Land

[Objective] This study aimed to investigate the artificial vegetations on soil physicochemical properties of sandy land. [Method] The soil physicochemical proper- ties in five representative lands respectively covered by Artemisia ordosica, Salix cheilophila, Hedysarum scoparium, Populus simonii and Amorpha fruticosa, all of which were planted artificially at the same year were measured in the present study, using a bare soil as the control. [Result] Artificial vegetation improved the soil physicochemical properties by different extents in the lands covered by different plants. The soil physicochemical properties such as bulk density under A. Fruticosa and H. Scoparium were improved greatly. The frequency distribution of soil particle size under artificial vegetations exhibited a bimodal curve. The average soil particle size under A. fruticosa was the smallest, and the soil was very poorly sorted. The soil nutrients in the sandy land were not significantly improved by artificial vegeta- tion. [Conclusion] Artificial vegetation has a certain impact on soil properties in sandy land, as it greatly improves the soil physical properties but not the chemical properties.

Available Contents of Trace Nutrient Element and Factors Affecting Their Availability in Western Jilin

Based on the assay of the total content and available content of the trace nutrient elements and the soil pH and organic matter of the soil samples,the characteristic of the available content of the trace nutrient elements and their affecting factors are studied. The results show that the available B in western Jilin is in a middle level,the content in Nong'an is higher than that in the others; the available Mn is extremely abundant; the available Cu in Nong'an is obviously higher than other areas,and the content in Da'an differs greatly; the available Zn is in a middle level; the severe shortage of available Mo differs between different areas; and the available Fe is extremely abundant. The available Mn,Cu and Fe have significantly positive correlation with their total content; the available B has significantly positive correlation with pH,and Zn has obviously negative correlation with pH; the available Mn and Fe had significantly positive correlation with soil organic matter.

Comprehensive Evaluation of Yield Characters and Quality Characters of New Cotton Varieties in Mid-south Area of Hebei

The yield characters and quality characters of several new cotton varieties in the mid-south area of Hebei Province were comprehensively evaluated in this pa- per. In different ecological environments, the single-boll weight of tested cotton vari- eties had the biggest variation coefficient among yield characters and micronaire value had the biggest variation coefficient among fiber quality characters. Path analy- sis indicated that the direct path coefficients of various characters had the flowing order, lint percentage〉 number of bolls per plant〉 single-boll weight〉 seed index among yield components and uniformity index 〉 fiber specific strength〉 micronaire value〉 elongation rate〉 upper half staple length among fiber quality characters. Principal component analysis indicated that the top three principal components contributed 8.263% of accumulated variance, basically reflecting the vast majority of informations of 10 main characters in tested cotton varieties.

Effects of Different Land Use Types on Soil Organic Carbon and Carbon Management Index in Karst Area

[Objective] The aim was to reveal changes of soil organic matter fraction and their corresponding carbon management indexes as affected by different land use types.[Method]Soil organic carbon,active soil organic carbon and soil carbon management index（CMI）of different land use types in Guilin Maocun karst area were studied.Sampling with field investigation and laboratory testing was carried out.Heavy potassium chromate method was adopted to determine soil organic matter.333 mmol/L KMnO4 oxidation method was used to determine active organic carbon.[Result]With active soil organic matter increasing,the differences of CMI between different land use types were bigger.The CMI value of different land uses was shrubforest paddy fielddry farmland.The statistical analysis showed that labile organic matter was related with major soil properties at a significant level.[Conclusion]Labile organic matter could be used to reveal the influence of different land use types on soil organic matter and carbon management index in karst area.

Geotechnical characterization of red shale and its indication for ground control in deep underground mining

Geotechnical properties of red shale encountered in deep underground mining were characterized on both laboratory and field scale to reveal its unfavorably in geoenvironment.Its constituents,microstructure,strength properties and water-weakening properties were investigated.In situ stress environment and mining-induced fractured damage zone after excavation were studied to reveal the instability mechanism.The results show that red shale contains swelling and loose clayey minerals as interstitial filling material,producing low shear strength of microstructure and making it vulnerable to water.Macroscopically,a U-shaped curve of uniaxial compressive strength(UCS)exists with the increase of the angle between macro weakness plane and the horizon.However,its tensile strength reduced monotonically with this angle.While immersed in water for72h,its UCS reduced by91.9%comparing to the natural state.Field sonic tests reveal that an asymmetrical geometrical profile of fractured damage zone of gateroad was identified due to geological bedding plane and detailed gateroad layout with regards to the direction of major principle stress.Therefore,red shale is a kind of engineering soft rock.For ground control in underground mining or similar applications,water inflow within several hours of excavation must strictly be prevented and energy adsorbing rock bolt is recommended,especially in large deformation part of gateroad.

Geological structure of the yellow sea area from regional gravity and magnetic interpretation

Regional gravity and aeromagnetic data covering the area of 32°- 38° N, 118°-127° E at the scale of 1：1,000,000 are coordinated and integrated in a synthetic study of the South China Yellow Sea and adjacent areas. Depth to magnetic crystalline basement and its structure are determined by magnetic anomaly inversion. Depth to and thickness of the Paleozoic rock are also revealed by gravity anomaly inversion with constrains of the basement and known seismic information from several profiles. Structure units, main faults, basin boundaries, and sub-suppressions are outlined on the basis of gravity data interpretation.

Assessment of potential impacts to surface and subsurface water bodies due to longwall mining

Ground movements due to longwall mining operations have the potential to damage the hydrological balance within as well as outside the mine permit area in the form of increased surface ponding and changes to hydrogeological properties. Recently, the Office of Surface Mining, Reclamation and Enforcement(OSMRE) in the USA, has completed a public comment period on a newly proposed rule for the protection of streams and groundwater from adverse impacts of surface and underground mining operations(80 FR 44435). With increased community and regulatory focus on mining operations and their potential to adversely affect streams and groundwater, now there is a greater need for better prediction of the possible effects mining has on both surface and subsurface bodies of water. With mining induced stress and strain within the overburden correlated to changes in the hydrogeological properties of rock and soil, this paper investigates the evaluation of the hydrogeological system within the vicinity of an underground mining operation based on strain values calculated through a surface deformation prediction model. Through accurate modeling of the pre- and post-mining hydrogeological system, industry personnel can better depict mining induced effects on surface and subsurface bodies of water aiding in the optimization of underground extraction sequences while maintaining the integrity of water resources.

Spatial Heterogeneity of Soil Mineral Oxide Components in Depression Between Karst Hills,Southwest China

In karst regions,the spatial heterogeneity of soil mineral oxides and environmental variables is still not clear.We investigated the spatial heterogeneity of SiO2,Al2O3,Fe2O3,CaO,MgO,P2O5,K2O,and MnO contents in the soils of slope land,plantation forest,secondary forest,and primary forest,as well as their relationships with environmental variables in a karst region of Southwest China.Geostatistics,principal component analysis(PCA),and canonical correlation analysis(CCA)were applied to analyze the field data.The results show that SiO2was the predominant mineral in the soils(45.02%–67.33%),followed by Al2O3and Fe2O3.Most soil mineral oxide components had a strong spatial dependence,except for CaO,MgO,and P2O5in the plantation forest,MgO and P2O5in the secondary forest,and CaO in the slope land.Dimensionality reduction in PCA was not appropriate due to the strong spatial heterogeneity in the ecosystems.Soil mineral oxide components,the main factors in all ecosystems,had greater influences on vegetation than those of conventional soil properties.There were close relationships between soil mineral oxide components and vegetation,topography,and conventional soil properties.Mineral oxide components affected species diversity,organic matter and nitrogen levels.

Geographic variation and genetic performance of Picea koraiensis in growth and wood characteristics

Eight provenances of 19-year-old Picea koraiensis Nakai from the provenancetrials of Maoershan (45°20′N, 127°30′E), Liang-shui (47°10′N, 128°53′E) and Jiagedaqi(50°24′N, 124°07′E) in Northeast China were investigated to analyze the genetic variation ingrowth characteristics (tree height and diameter) and wood characteristics (tracheid length,tracheid diameter, tracheid wall thickness, annual ring width as well as wood density). Greatvariation in height growth and breast height diameter growth was observed among the provenances, andalong with the increase of tree age, these provenances presented different geographic adaptability.The growth characteristics of Picea koraiensis stand at age of 10 in Maoershan and Liangshuiprovenance trials had a positive correlation with longitude, and with increase of tree age to 15 and19, the tree growth of the provenances displayed a significant positive correlation with latitudeas well as altitude. For wood characteristics, great variation was also found among the provenances.There exists a close relation between growth characteristics and wood properties of the provenance.The height and breast height diameter growth of the provenance had a positive correlation withtracheid diameter and annual ring width, and a negative correlation with tracheid wall thickness andwood density. Genetic performance of the provenance in all above characteristics was alsoinvestigated in order to provide more useful information for comprehensive selection of this speciesfor pulpwood and plywood production.

Implicit modeling of complex orebody with constraints of geological rules

To dynamically update the shape of orebody according to the knowledge of a structural geologist’s insight,an approach of orebody implicit modeling from raw drillhole data using the generalized radial basis function interpolant was presented.A variety of constraint rules,including geology trend line,geology constraint line,geology trend surface,geology constraint surface and anisotropy,which can be converted into interpolation constraints,were developed to dynamically control the geology trends.Combined with the interactive tools of constraint rules,this method can avoid the shortcomings of the explicit modeling method based on the contour stitching,such as poor model quality,and is difficult to update dynamically,and simplify the modeling process of orebody.The results of numerical experiments show that the 3D ore body model can be reconstructed quickly,accurately and dynamically by the implicit modeling method.

Groundwater resources survey of tongchuan city using the audio magnetotelluric method

The development of Tongchuan City,Shaanxi Province,located in the northwestern region of China,is restricted by water resources.The direct current resistivity and induced polarization sounding methods are typically applied in finding urban groundwater.These methods,however,are not effective due to their complicated topography and geological conditions.The application practice shows that the audio magnetotelluric(AMT)method has a large depth of exploration,high work effi ciency,and high lateral resolution.To investigate the distribution of groundwater resources,we deployed three audio-frequency magnetotelluric profiles in the city area.The impedance tensor information of AMT data is obtained using SSMT2000.AMT data dimension analysis reveals that the two-dimensional structural features of the observation area are obvious.The main structure of the observation area is about 45°northeast,as indicated by structural trend analysis.A shallow two-dimensional electrical profile of 1 km in Tongchuan City is obtained by two-dimensional nonlinear conjugate gradient inversion.Finally,combined with regional geological information,the geological structure characteristics reflected by the electrical profile were obtained along with the detailed characteristics of water-rich structures in the area.The infl uence of the structure on the groundwater distribution was analyzed,and the water-rich areas were identifi ed.This work contributes to the prospective development of Tongchuan City.

Aggregate Stability and Its Relationship with Some Chemical Properties of Red Soils in Subtropical China

The stability of aggregates in the surface soil is crucial to soil erosion and runoff generation. Thus, to understand the stability and the breakdown mechanisms of soil aggregates as well as the relationship between aggregate stability and selected soil chemical properties, such as different forms of Fe and Al oxides, organic matter, CEC and clay content, the aggregates of slightly and severely eroded red soils derived from Quaternary red clay in subtropical China were analyzed using the routine wet sieving…