Objective: To explore the expression of Th1/Th2 cytokines gene in human gliomas and its role in the genesis and development of human gliomas.Methods: Using IL-2 and IFNγ as Th1 type cytokines, IL-4, IL-6 and IL-10 as...Objective: To explore the expression of Th1/Th2 cytokines gene in human gliomas and its role in the genesis and development of human gliomas.Methods: Using IL-2 and IFNγ as Th1 type cytokines, IL-4, IL-6 and IL-10 as Th2 type cytokines, the biological activity of cytokines in the supernatant of glioma cell lines was assayed by ELISA method, and the gene expression of Th1/Th2 cytokines in human glioma cells, glioma infiltrating lymphocytes and glioma cell lines were detected by RT-PCR.Results: There was predominant expression of Th2 type cytokines in human glioma cells, glioma infiltrating lymphocytes and glioma cell lines, but there was no such expression in normal brain tissues.Conclusion: It suggested that there is a relationship between the Th2 type cytokines expression in human gliomas and the immunosupressive status of human glioma patients. The predominant expression of Th2 type cytokines may play an important role in the genesis and development of human gliomas. Key words glioma - Th1/Th2 - gene expression - RT-PCR This project was supported by a grant from National Natural Sciences foundation of China (No. 30271335).展开更多
Al/Co co-doped α-Ni（OH）2 samples were prepared by either ultrasonic co-precipitation method （Sample B） or co-precipitation method （Sample A）. The crystal structure and particle size distribution of the prepared...Al/Co co-doped α-Ni（OH）2 samples were prepared by either ultrasonic co-precipitation method （Sample B） or co-precipitation method （Sample A）. The crystal structure and particle size distribution of the prepared samples were examined by X-ray diffraction （XRD） and laser particle size analyzer, respectively. The results show that Sample B has more crystalline defects and smaller average diameter than Sample A. The cyclic voltammetry and electrochemical impedance spectroscopy measurements indicate that Sample B has better electrochemical performance than Sample A, such as better reaction reversibility, lower charge-transfer resistance and better cyclic stability. Proton diffusion coefficient of Sample B is 1.96×10-10cm2/s, which is two times as large as that （9.78×10-11cm2/s） of Sample A. The charge-discharge tests show that the discharge capacity （308 mA·h/g） of Sample B is 25 mA·h/g higher than that of Sample A （283 mA·h/g）.展开更多
Spherical Ni（OH）2 particles were prepared by an aqueous solution precipitation route. The structure of spherical Ni（OH）2 was investigated by scanning electron microscopy and transmission electron microscopy and co...Spherical Ni（OH）2 particles were prepared by an aqueous solution precipitation route. The structure of spherical Ni（OH）2 was investigated by scanning electron microscopy and transmission electron microscopy and compared with that of traditional Ni（OH）2. The results show that the spherical nickel hydroxide consists of （Ni（OH）2） spheres with a reticulate structure of platelet-like, which is almost arranged radially and the crystalline grains intervene and connect with each other to form a three-dimensional net. The spherical Ni（OH）2 particle is full of pores, crannies between cleave planes. It is supposed that this structure is beneficial to the structural stability for the spherical particles during the charge/discharge processes and can improve the cycle life of the electrode; the pores and the crannies in spherical particles can shorten the proton diffusion distance and speed its velocity, which may result in that the local polarization is lowered. The electrochemical performances of the spherical Ni（OH）2 are improved by enhancing the conducting properties of the crystalline lattice due to its quick proton diffusion.展开更多
The structural and electronic properties of Li2Mg（NH）2 for hydrogen storage have been studied by first-principles calculation. The optimal unit cell parameters and the distance of N-H are determined, which are in go...The structural and electronic properties of Li2Mg（NH）2 for hydrogen storage have been studied by first-principles calculation. The optimal unit cell parameters and the distance of N-H are determined, which are in good agreement with the experimental data. The bulk modules and the energies of zero pressure are obtained by using Murnaghan equation of states. The results show that the α-Li2Mg（NH）2 is a ground state configuration. The overlap population analysis shows that the N-Li/Mg ionic characteristics and N-H interaction of αphase are weaker than those of βphase. The valence band is dominated by the presence of N s and p states, hybridized with the H s state.展开更多
To investigate the effect of doxorubicin(DOX) on gene expression of the myocardial sarcoplasmic reticulum (SR)Ca 2+ transport proteins and the mechanism of taurine(Tau) protecting cardiac muscle cells, 9 rabbits...To investigate the effect of doxorubicin(DOX) on gene expression of the myocardial sarcoplasmic reticulum (SR)Ca 2+ transport proteins and the mechanism of taurine(Tau) protecting cardiac muscle cells, 9 rabbits were injected with DOX , 8 rabbits with DOX and Tau, and 9 rabbits with normal saline. Cardiac function , concentration of calcium in cardiomyocytes (Myo[Ca 2+ ] \%i\%), activity of SR Ca 2+ ATPase(SERCA2a), level of SERCA2a mRNA and Ca 2+ released channels(RYR2)mRNA were detected. The left ventricle tissues were observed by electron microscopy. The results showed that cardiac index, left ventricular systolic pressure, activity of SR Ca 2+ ATPase and level of SERCA2a mRNA decreased , while Myo[Ca 2+ ] \%i\% increased in DOX treated rabbits. DOX could not affect the level of RYR2 mRNA. Tau intervention could alleviate the increase of left ventricular diastolic pressure, Myo[Ca 2+ ] \%i\% and the decrease of SERCA2a mRNA induced by doxorubicin. The results suggested that downregulation of SERCA2a gene expression was an important mechanism of DOX induced cardiomyopathy and that Tau could partially improve the heart function by reducing calcium overload and alleviating downregulation of SERCA2a mRNA.展开更多
Excited-state double proton transfer(ESDPT)is a controversial issue which has long been plagued with theoretical and experimental communities.Herein,we took 1,8-dihydroxy-2-naphthaldehyde(DHNA)as a prototype and used ...Excited-state double proton transfer(ESDPT)is a controversial issue which has long been plagued with theoretical and experimental communities.Herein,we took 1,8-dihydroxy-2-naphthaldehyde(DHNA)as a prototype and used combined complete active space selfconsistent field(CASSCF)and multi-state complete active-space second-order perturbation(MS-CASPT2)methods to investigate ESDPT and excited-state deactivation pathways of DHNA.Three different tautomer minima of S1-ENOL,S1-KETO-1,and S1-KETO-2 and two crucial conical intersections of S1 S0-KETO-1 and S1 S0-KETO-2 in and between the S0 and S1 states were obtained.S1-KETO-1 and S1-KETO-2 should take responsibility for experimentally observing dual-emission bands.In addition,two-dimensional potential energy surfaces(2 D-PESs)and linear interpolated internal coordinate paths connecting relevant structures were calculated at the MS-CASPT2//CASSCF level and confirmed a stepwise ESDPT mechanism.Specifically,the first proton transfer from S1-ENOL to S1-KETO-1 is barrierless,whereas the second one from S1-KETO-1 to S1-KETO-2 demands a barrier of ca.6.0 kcal/mol.The linear interpolated internal coordinate path connecting S1-KETO-1(S1-KETO-2)and S_(1) S0-KETO-1(S1 S0-KETO-2)is uphill with a barrier of ca.12.0 kcal/mol,which will trap DHNA in the S_(1) state while therefore enabling dual-emission bands.On the other hand,the S1/S0 conical intersections would also prompt the S_(1) system to decay to the S_(0) state,which could be to certain extent suppressed by locking the rotation of the C5-C8-C9-O10 dihedral angle.These mechanistic insights are not only helpful for understanding ESDPT but also useful for designing novel molecular materials with excellent photoluminescent performances.展开更多
Tongting coal(TTC) was exhaustively extracted with carbon disulfide and N-melthy-2-pyrolidinone(CS 2 /NMP) mixed solvents to afford brown particles of extract,which was characterized with proximate analyzer,transmissi...Tongting coal(TTC) was exhaustively extracted with carbon disulfide and N-melthy-2-pyrolidinone(CS 2 /NMP) mixed solvents to afford brown particles of extract,which was characterized with proximate analyzer,transmission electron microscope(TEM) and Fourier transform infrared(FTIR) spectrometer.The results show that the nanometer particles of extract,which were free of ash,are superfine and superclean with tract content of 0.02% A d and particles size of about 100-150 nm.TTC and extract were then subject to oxidation with H 2 O 2 and oxidation products were subsequently analyzed with FTIR and gas chromatography/mass spectrometer(GC/MS).The results show that extract is more reactive with H 2 O 2 in comparison to TTC and richer in oxygen-containing species including phenols,alcohols,ethers,esters,carboxylic acids and anhydrides.展开更多
基金This project was supported by a grant from National Natural Sciences foundation of China(No.30271335).
文摘Objective: To explore the expression of Th1/Th2 cytokines gene in human gliomas and its role in the genesis and development of human gliomas.Methods: Using IL-2 and IFNγ as Th1 type cytokines, IL-4, IL-6 and IL-10 as Th2 type cytokines, the biological activity of cytokines in the supernatant of glioma cell lines was assayed by ELISA method, and the gene expression of Th1/Th2 cytokines in human glioma cells, glioma infiltrating lymphocytes and glioma cell lines were detected by RT-PCR.Results: There was predominant expression of Th2 type cytokines in human glioma cells, glioma infiltrating lymphocytes and glioma cell lines, but there was no such expression in normal brain tissues.Conclusion: It suggested that there is a relationship between the Th2 type cytokines expression in human gliomas and the immunosupressive status of human glioma patients. The predominant expression of Th2 type cytokines may play an important role in the genesis and development of human gliomas. Key words glioma - Th1/Th2 - gene expression - RT-PCR This project was supported by a grant from National Natural Sciences foundation of China (No. 30271335).
基金Project (10774030) supported by the National Natural Science Foundation of ChinaProject (2008J1-C161) supported by the Science and Technology Program of Guangzhou City of China
文摘Al/Co co-doped α-Ni（OH）2 samples were prepared by either ultrasonic co-precipitation method （Sample B） or co-precipitation method （Sample A）. The crystal structure and particle size distribution of the prepared samples were examined by X-ray diffraction （XRD） and laser particle size analyzer, respectively. The results show that Sample B has more crystalline defects and smaller average diameter than Sample A. The cyclic voltammetry and electrochemical impedance spectroscopy measurements indicate that Sample B has better electrochemical performance than Sample A, such as better reaction reversibility, lower charge-transfer resistance and better cyclic stability. Proton diffusion coefficient of Sample B is 1.96×10-10cm2/s, which is two times as large as that （9.78×10-11cm2/s） of Sample A. The charge-discharge tests show that the discharge capacity （308 mA·h/g） of Sample B is 25 mA·h/g higher than that of Sample A （283 mA·h/g）.
基金Project(50134020) supported by the National Natural Science Foundation of China
文摘Spherical Ni（OH）2 particles were prepared by an aqueous solution precipitation route. The structure of spherical Ni（OH）2 was investigated by scanning electron microscopy and transmission electron microscopy and compared with that of traditional Ni（OH）2. The results show that the spherical nickel hydroxide consists of （Ni（OH）2） spheres with a reticulate structure of platelet-like, which is almost arranged radially and the crystalline grains intervene and connect with each other to form a three-dimensional net. The spherical Ni（OH）2 particle is full of pores, crannies between cleave planes. It is supposed that this structure is beneficial to the structural stability for the spherical particles during the charge/discharge processes and can improve the cycle life of the electrode; the pores and the crannies in spherical particles can shorten the proton diffusion distance and speed its velocity, which may result in that the local polarization is lowered. The electrochemical performances of the spherical Ni（OH）2 are improved by enhancing the conducting properties of the crystalline lattice due to its quick proton diffusion.
基金ACKNOWLEDGMENTS This work is supported by the National Natural Science Foundation of China （No.20876005）. Computational resources were supported by the ＂Chemical GridProject＂ of Beijing University of Chemical Technology.
文摘The structural and electronic properties of Li2Mg（NH）2 for hydrogen storage have been studied by first-principles calculation. The optimal unit cell parameters and the distance of N-H are determined, which are in good agreement with the experimental data. The bulk modules and the energies of zero pressure are obtained by using Murnaghan equation of states. The results show that the α-Li2Mg（NH）2 is a ground state configuration. The overlap population analysis shows that the N-Li/Mg ionic characteristics and N-H interaction of αphase are weaker than those of βphase. The valence band is dominated by the presence of N s and p states, hybridized with the H s state.
文摘To investigate the effect of doxorubicin(DOX) on gene expression of the myocardial sarcoplasmic reticulum (SR)Ca 2+ transport proteins and the mechanism of taurine(Tau) protecting cardiac muscle cells, 9 rabbits were injected with DOX , 8 rabbits with DOX and Tau, and 9 rabbits with normal saline. Cardiac function , concentration of calcium in cardiomyocytes (Myo[Ca 2+ ] \%i\%), activity of SR Ca 2+ ATPase(SERCA2a), level of SERCA2a mRNA and Ca 2+ released channels(RYR2)mRNA were detected. The left ventricle tissues were observed by electron microscopy. The results showed that cardiac index, left ventricular systolic pressure, activity of SR Ca 2+ ATPase and level of SERCA2a mRNA decreased , while Myo[Ca 2+ ] \%i\% increased in DOX treated rabbits. DOX could not affect the level of RYR2 mRNA. Tau intervention could alleviate the increase of left ventricular diastolic pressure, Myo[Ca 2+ ] \%i\% and the decrease of SERCA2a mRNA induced by doxorubicin. The results suggested that downregulation of SERCA2a gene expression was an important mechanism of DOX induced cardiomyopathy and that Tau could partially improve the heart function by reducing calcium overload and alleviating downregulation of SERCA2a mRNA.
基金supported by the National Key Research and Development Program of China for BinBin Xie(No.2019YFA0709400)the National Natural Science Foundation of China for Bin-Bin Xie(No.21903068)+1 种基金Xiang-Yang Liu(No.22003043)Natural Science Foundation of Zhejiang Province for Bin-Bin Xie(No.LQ19B030007)。
文摘Excited-state double proton transfer(ESDPT)is a controversial issue which has long been plagued with theoretical and experimental communities.Herein,we took 1,8-dihydroxy-2-naphthaldehyde(DHNA)as a prototype and used combined complete active space selfconsistent field(CASSCF)and multi-state complete active-space second-order perturbation(MS-CASPT2)methods to investigate ESDPT and excited-state deactivation pathways of DHNA.Three different tautomer minima of S1-ENOL,S1-KETO-1,and S1-KETO-2 and two crucial conical intersections of S1 S0-KETO-1 and S1 S0-KETO-2 in and between the S0 and S1 states were obtained.S1-KETO-1 and S1-KETO-2 should take responsibility for experimentally observing dual-emission bands.In addition,two-dimensional potential energy surfaces(2 D-PESs)and linear interpolated internal coordinate paths connecting relevant structures were calculated at the MS-CASPT2//CASSCF level and confirmed a stepwise ESDPT mechanism.Specifically,the first proton transfer from S1-ENOL to S1-KETO-1 is barrierless,whereas the second one from S1-KETO-1 to S1-KETO-2 demands a barrier of ca.6.0 kcal/mol.The linear interpolated internal coordinate path connecting S1-KETO-1(S1-KETO-2)and S_(1) S0-KETO-1(S1 S0-KETO-2)is uphill with a barrier of ca.12.0 kcal/mol,which will trap DHNA in the S_(1) state while therefore enabling dual-emission bands.On the other hand,the S1/S0 conical intersections would also prompt the S_(1) system to decay to the S_(0) state,which could be to certain extent suppressed by locking the rotation of the C5-C8-C9-O10 dihedral angle.These mechanistic insights are not only helpful for understanding ESDPT but also useful for designing novel molecular materials with excellent photoluminescent performances.
基金subsidized by the National Natural Science Foundation of China (Nos.50474066,50874108 and 50921002)the Fundamental Research Funds for the Central Universities(No.2010LKHX01)the National Basic Research Program of China (No.2012CB214900)
文摘Tongting coal(TTC) was exhaustively extracted with carbon disulfide and N-melthy-2-pyrolidinone(CS 2 /NMP) mixed solvents to afford brown particles of extract,which was characterized with proximate analyzer,transmission electron microscope(TEM) and Fourier transform infrared(FTIR) spectrometer.The results show that the nanometer particles of extract,which were free of ash,are superfine and superclean with tract content of 0.02% A d and particles size of about 100-150 nm.TTC and extract were then subject to oxidation with H 2 O 2 and oxidation products were subsequently analyzed with FTIR and gas chromatography/mass spectrometer(GC/MS).The results show that extract is more reactive with H 2 O 2 in comparison to TTC and richer in oxygen-containing species including phenols,alcohols,ethers,esters,carboxylic acids and anhydrides.
