放射性同位素质控物质自动切换关键技术研究

为了解决表面污染仪检测过程的工作效率低和不利于辐射安全防护的问题,文章从检测实际出发,设计了一种放射性同位素质控物质自动切换装置,并对放射性同位素质控物质自动切换关键技术进行研究.对步进电机驱动进行了设计,实现了同位素质控物质的切换定位,并对其切换的速度进行了控制.文章的研究可以提升表面污染仪检测过程的工作效率,并且也解决了辐射安全防护的问题,为计量检测部门以及相关机构开展表面污染仪质检提供了技术保障.

水产品扇贝中沙门氏菌检测方法的优化

对水产品扇贝中的沙门氏菌进行检测,用其阳性物质进行质量控制,避免了使用标准菌株的繁琐,检测结果准确可靠。

Response of Amaranth to Irrigation and Organic Matter

The study investigated the response of Amaranth to irrigation depth and irrigation interval as well as poultry waste incorporation. A replicated 4 ×3 × 3 factorial arrangement with treatments consisting of percent organic matter incorporation （0%, 2%, 4% and 6% by weight）, irrigation interval （1, 3 and 5 days） and irrigation depth （4, 6 and 8 mm） was used for the experiment which was conducted in pots in a green house. The result of the experiment showed that percent organic matter incorporation, irrigation interval, and irrigation depth significantly （P 〈 0.05） affected the dry matter yield of the vegetable. However, only the percent organic matter incorporation and irrigation interval were significant （P 〈 0.05） in their effects on the plant height. The highest plant height of 12.03 cm occurred when 2% organic matter was incorporated into the soil while the lowest plant height of 9.04 cm occurred with 6% organic matter incorporation. The maximum plant yield （47.44 kg/ha） occurred when 2% poultry litter was incorporated into the soil while the lowest plant yield （31.57 kg/ha） occurred with the control experiment. Irrigation interval of three days as well as irrigation depth of 6 mm resulted in the highest plant height and maximum dry matter yield of the vegetable. From the result of the experiment, the factor combination of 2% organic matter incorporation with 6 mm irrigation depth applied at three days irrigation interval resulted in the highest plant height of 16.7 cm above ground surface and maximum yield of 67.8 kg/ha.

风疹病毒IgG抗体参考品的建立

目的:建立人风疹病毒IgG抗体参考盘,为风疹病毒IgG抗体检测试剂盒的性能评价提供可靠的质控物质。方法:通过风疹病毒IgG抗体检测试剂盒进行血清筛选和复核,免疫荧光方法和Western blot方法对筛选的血清进行确认,获得了符合要求的血清,组成了含有10份阳性血清和5份阴性血制成参考品的P1～P10和N1～N5、重复性参考品R以及检测限参考品L1,共有17支参考品的参考盘。经过不同方法学的性能验证,确立风疹病毒IgG抗体参考盘的性能指标,并且参考盘的稳定性进行测试。结果:经风疹病毒IgG抗体检测试剂盒初筛和复核、以及免疫荧光法和Western blot方法确认,确定10份风疹病毒IgG阳性血清样本和5份阴性血清样本,选择其中一份阳性样本作为重复性参考品,10 IU.mL-1作为检测限参考品,构建成人风疹病毒IgG抗体参考盘。确定了参考盘的性能指标:1.准确性(阳性符合率):检测国家阳性参考品10份样本,要求假阴性应不得出现。2.特异性(阴性符合率):检测国家阴性参考品5份样本,要求假阳性应不得出现。3.重复性(精密度):批内精密度RSO不大于15.0%。4.检测限:用风疹病毒IgG抗体检测试剂对国家最低检出限参考品进行检测,要求L1应能检出。稳定性研究结果表明,参考盘的稳定性良好。成功地建立了人风疹病毒IgG抗体国家参考盘。结论:该参考盘为临床实验室质量评价提供了可靠的参考物质,可用于国内大多数试剂盒的性能评价要求。