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液相色谱法测定纺织品中甾醇含量
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作者 贺志鹏 刘蒙 《国际纺织导报》 2024年第2期24-27,共4页
通过确定植物甾醇3种主要成分(β-谷甾醇、菜油甾醇、豆甾醇)标准品的液相色谱分析条件,以特征保留时间确定各类甾醇相应的液相色谱指纹图谱。建立了纺织品中甾醇含量的测定方法:以相同液相色谱分析条件对纺织样品提取液进样分析,以各... 通过确定植物甾醇3种主要成分(β-谷甾醇、菜油甾醇、豆甾醇)标准品的液相色谱分析条件,以特征保留时间确定各类甾醇相应的液相色谱指纹图谱。建立了纺织品中甾醇含量的测定方法:以相同液相色谱分析条件对纺织样品提取液进样分析,以各类甾醇标准品指纹图谱为基础,并以外标法求得纺织品中不同类别甾醇含量,最终获得纺织品中甾醇总的含量(质量分数)。测定方法精密度和回收率均满足相关标准要求,可用于纺织品中甾醇含量的检测。 展开更多
关键词 液相色谱法 纺织品 甾醇 含量(质量分数)测定
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火焰原子吸收分光光度法测定丙戊酸钠片含量
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作者 付霞 王家芳 +1 位作者 田朋鑫 徐树娟 《山东化工》 CAS 2021年第24期142-144,共3页
目的:建立火焰原子吸收分光光度法测定丙戊酸钠片质量含量。方法:检测波长:589.0 nm;通带:0.2 nm;溶剂:1%硝酸溶液;火焰类型:空气-乙炔;燃气流量:1.1 L/min。结果:丙戊酸钠在质量浓度0.5~5μg/mL的范围内线性关系良好,线性回归方程为Y=0... 目的:建立火焰原子吸收分光光度法测定丙戊酸钠片质量含量。方法:检测波长:589.0 nm;通带:0.2 nm;溶剂:1%硝酸溶液;火焰类型:空气-乙炔;燃气流量:1.1 L/min。结果:丙戊酸钠在质量浓度0.5~5μg/mL的范围内线性关系良好,线性回归方程为Y=0.0926X+0.0214,r=0.9979;重复性RSD=0.6%;回收率为101.4%;辅料不干扰丙戊酸钠片质量含量测定结果,专属性良好;24 h内吸光度稳定,RSD=1.2%;两批丙戊酸钠片的质量含量测定结果分别为100.4%,100.1%,与药典方法测定的结果相比,无显著差异。结论:经验证,该方法线性、重复性、回收率、专属性、稳定性良好,可用于测定丙戊酸钠片中丙戊酸钠质量含量。 展开更多
关键词 火焰原子吸收分光光度法 丙戊酸钠 质量含量测定
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Determination of Isoflavone from Soybean Lines Cultivated in Jilin Province and Correlation Analysis between Isoflavone Content and Soybean Quality 被引量:29
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作者 宋向东 张之鑫 +1 位作者 王巍巍 富健 《Agricultural Science & Technology》 CAS 2010年第1期48-50,共3页
[Objective]The aim of this study was to set up a high performance liquid chromatography for rapid determination of isoflavones from soybean and analyze the correlation between isofalvone content and protein or fat con... [Objective]The aim of this study was to set up a high performance liquid chromatography for rapid determination of isoflavones from soybean and analyze the correlation between isofalvone content and protein or fat content. [Method]The isoflavones were firstly extracted by 80% methanol and then hydrolyzed at 100 ℃. The chromatographic separation adopted a reversed-phase C18 analytical column with binary high-pressure gradient elution,while its analysis time was 25 min and column temperature was 40 ℃. The diode array detector was used for monitoring with wavelength of 260 nm. The correlation between isofalvone content and protein or fat content was analyzed by data processing system Origin 6.0. [Result]The high performance liquid chromatograph for determination of isoflavones from soybean was verified to be accurate and reliable by methodology. The isoflavones of 85 soybean lines cultivated in Jilin Province were determined,and the results primarily showed the characters and ranges of isoflavones from soybean lines cultivated in Jilin Province,while the isoflavone content of soybeans ranged from 2.29 to 4.89 mg/g,and the average content was 3.36 mg/g. The isoflavone content of 5 soybean lines exceeded 4 mg/g,while there was a remarkably negative correlation between isoflavone content and protein content,and there was no significant positive correlation between isoflavone content and fat content. [Conclusion]The isoflavone content of soybean lines cultivated in Jilin Province is higher,so it is feasible for breeding the soybean lines with high isoflavone content and fat contetnt. 展开更多
关键词 Cultivated soybean Isoflavone content DETERMINATION Protein content Fat content
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Exploration of Urinary Creatinine to Determine the Carcass and Its Protein Weight in Beef Cattle
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作者 A. Purnomoadi T. Wahyuningtyas E. Rianto 《Journal of Agricultural Science and Technology(A)》 2011年第8期1159-1161,共3页
This study was aimed to explore the use of creatinine as indicator to predict carcass and its protein weight in beef cattle. Eight Ongole crossbred cattle with initial body weight ranged at 133.5-228 kg and age of 6-1... This study was aimed to explore the use of creatinine as indicator to predict carcass and its protein weight in beef cattle. Eight Ongole crossbred cattle with initial body weight ranged at 133.5-228 kg and age of 6-18 months were used in this study. The cattle were fed Napier grass ad libitum and concentrate feeding for three months prior to be slaughtered. Concentrate feeding was consisted of rice bran and soybean meal which was provided to fulfill dry matter requirement at 2.1% of body weight (BW). The availability of creatinine for prediction indicator was done by evaluate the correlation between the amount of daily urinary creatinine and the carcass and its protein weight. Carcass and its protein weight were measured by slaughtering the cattle, and chemically analyzed for determining protein content of carcass. The results showed that creatinine excreted in urine have a strong correlation with the cattle body weight (r = 0.88), carcass weight (r = 0.67), body protein (r = 0.70) and carcass protein (r = 0.72). The conclusion of this study is creatinine excreted in urine have a strong relationship with the carcass and its protein, and therefore could be used to predict the carcass and its protein weight of beef cattle. 展开更多
关键词 Beef cattle CREATININE carcass weight carcass protein body weight
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Application of UHPLCMS/ MS method in determining the content of multiingredient in hospital preparations
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作者 林志燕 杨荣富 +2 位作者 唐跃年 田怀平 张健 《Journal of Chinese Pharmaceutical Sciences》 CAS CSCD 2014年第4期233-240,共8页
In the present study, we established an ultra-high pressure liquid chromatography-tandem mass spectrometry (UHPLC-MS/MS) method to simultaneously determine 11 compounds in two hospital preparations (Shegan mixture ... In the present study, we established an ultra-high pressure liquid chromatography-tandem mass spectrometry (UHPLC-MS/MS) method to simultaneously determine 11 compounds in two hospital preparations (Shegan mixture and Gandi capsules), including ephedrine, caffeic acid, ferulic acid, rutin, scutellarin, belamcandin, baicalin, baicalein, astragaloside IV, irisflorentin and wogonin. The chromatographic separation was conducted on ZORBAX SB-C18 (2.1 mm×50 mm, 1.8 μm). Eleven analytes and IS were detected using ESI and MRM combined with positive and negative scanning switch. Caffeic acid, ferulic acid, scutellarin and belamcandin were detected using negative ion mode detection, whereas ephedrine, rutin, baicalin, baicalein, astragaloside IV, irisflorentin and wogonin were detected using positive ion mode detection. The quantification limits of ephedrine, caffeic acid, scutellarin, belamcandin, baicalin, baicalein, irisfiorentin and wogonin were 4.90×10^-3 ng/mL, 7.80 ng/mL, 6.8 ng/mL, 5.3×10^-2 ng/mL, 4.20×10^-3 ng/mL, 4.6×10^-2 ng/mL, 1.44×10^-4 ng/mL, 4.85 ng/mL, 0.23 ng/mL, 3.18× 10^-4 ng/mL and 2.95× 10^-4 ng/mL, respectively. The detection limits of these components were 2.90× 10^-4 ng/mL, 0.77 ng/mL, 2.0 ng/mL, 0.016 ng/mL, 1.3×10^-3 ng/mL, 3.33×10^-4 ng/mL, 4.32×10^-5 ng/mL, 1.46 ng/mL, 0.07 ng/mL, 9.5×10^-5 ng/mL and 8.84× 10^-5 ng/mL, respectively. All calibration curves showed good linearity (R^2〉0.99) within the test range. The RSD values of intra-day and inter-day precision were less than 5%, and the average recovery rates of the 11 components ranged fi'om 80% to 120%. In conclusion, our newly developed method was simple, rapid, sensitive and accurate. It could be used to determine ephedrine, caffeic acid, ferulic acid, rutin, scutellarin, belamcandin, baicalin, baicalein, astragaloside IV, irisflorentin and wogonin in Shegan mixture and Gandi capsules, which was helpful for the quality control of drugs. 展开更多
关键词 Shegan mixture Gandi capsules Ultra-high pressure liquid chromatography-tandem mass spectrometry Determination of multi-ingredient Quality control of Chinese patent drug
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