The fresh pollen vitality, the effect of different storage conditions on the pollen vitality, and the difference of vitality among the species of seven species of Syringa were determined in Shenyang, China. The result...The fresh pollen vitality, the effect of different storage conditions on the pollen vitality, and the difference of vitality among the species of seven species of Syringa were determined in Shenyang, China. The results indicated that the pollen vi-tality (81.5%) of Syringa villosa was the highest among the seven tested species, followed by S. microphylla and S. meyeri, and that of S. oblata var. affinis was the lowest. The low temperature was the best condition for storage of pollen of Syringa, and the most proper temperature for the storage was 0-2 癈. The storability of S. microphylla was the best of all, and it could be stored over 60 days at the temperature of 0-2 癈, next was S. villosa and S. meyeri.展开更多
To explore the change rule of seed vigor and seed application technology of rice PTGMS lines with low temperature storage,Zhun S,Y58S,Feng S and Guangzhan 63-4S stored in the seed bank with the temperature of 8-10 ℃ ...To explore the change rule of seed vigor and seed application technology of rice PTGMS lines with low temperature storage,Zhun S,Y58S,Feng S and Guangzhan 63-4S stored in the seed bank with the temperature of 8-10 ℃ and with the relative humidity of 55% for different years were used as the materials to study the properties of seed vigor,growth and development in the field, etc. The re- sults showed that the germination potential, germination rate, germination index and vigor index of rice PTGMS lines seeds showed downward trends as the time of low temperature storage prolonged,and all of the germination rates of the seeds within five years were over 60%. The conductivity in the soaked solution of seeds was significantly increased with the prolonged low temperature storage time. Seedling rate,root length,white root number,base stem width and seedling dry weight of seeds showed downward trends as the time of low temperature storage prolonged. There was no significant difference between two integral seedling qualities of seeds within 4-6 years in low temperature storage. Compared with the germination rate of low temperature storage seeds soaking for 6,12 and 24 h,the appropriate soak time of long time low temperature storage or low vigor seeds was 12 h. A serious natural aging phenomenon occurred in low temperature storage seeds after out of the storage. The germination rate of seeds placed under natural condition for 50 d decreased obviously. The duration from seeding to heading of Zhun S,Y58S and Feng S shortened as the time of low temperature storage prolonged, while the vari- ation of panicle duration of all PTGMS lines was not obvious. In conclusion, rice PTGMS line seeds with low temperature storage in 5 years could still be used nor- mally. However.seeds after low temperature storage should be sowed as soon as possible, and the soaking time of low vigor seeds should be shortened,and the possibility that the decreased duration from seeding to heading should also be con- sidered.展开更多
The greenhouse has been increasingly used in the breeding industry. However, the high temperature inside the greenhouse in summer has not been effectively addressed. The spray cooling system sprays tiny droplets into ...The greenhouse has been increasingly used in the breeding industry. However, the high temperature inside the greenhouse in summer has not been effectively addressed. The spray cooling system sprays tiny droplets into the air. Thus the water molecules will be vaporized, absorbing heat and reducing ambient temperature. It is the only cooling method that can be used to cool the uneasily-sealed flexible greenhouse. We developed an energy-storing high pressure spray cooling system. The ordinary water pump is used as the source of high-pressure water. The partial kinetic energy is stored in the energy-storing tubes. When the water pump is stopped, the energy produced by releasing the compressed air can still be used to maintain the spray. And thus the use-cost and systematic wear would be reduced. The cooling system only requires 1 kilowatt hour of power per day. It has been widely used in summer to cool the breeding sheds. After a recent continuous improvement, its functions have been extended to disinfection, removing dust, humidifying and immunizing animals. In addition, it can also be used for the cooling and humidifying of squares, venues and streets in summer. The energy-storing high pressure spray cooling system has a broad application prospect.展开更多
Aim To study the most stable pH value of endomorphin-1 solution and toestimate the shelf-life of EM-1 solution. Methods The content of EM-1 in its solution was determinedby HPLC, and stability was studied by accelerat...Aim To study the most stable pH value of endomorphin-1 solution and toestimate the shelf-life of EM-1 solution. Methods The content of EM-1 in its solution was determinedby HPLC, and stability was studied by accelerated tests. Results The most stable pH value of EM-1solution was observed. By the accelerated tests we obtained the Arrhe-nius relationship and thepharmacokinetic parameters. Conclusion The most stable pH value of EM-1 solution is 4.5 -4.8. Thet_(1/2)(25℃) is 45 d.展开更多
The effects of temperature, light, pH value and storage time on germina- tion of Adelostemma gracillimum seeds were studied through germination experi- ment. The results showed that A. gracillimum seeds had no propert...The effects of temperature, light, pH value and storage time on germina- tion of Adelostemma gracillimum seeds were studied through germination experi- ment. The results showed that A. gracillimum seeds had no property of post-matu- ration dormancy; the optimum germination temperature was 25 ~C; no significant difference was found in germination rate of A. gracillimum seeds between light and dark conditions; A. gracillimum seeds showed a wide adaptability to pH, Le., they could germinate in the pH range of 2-10, and showed a higher germination rate in the pH range of 6-8. The optimum storage time for A. gracillimum seeds was 12 months.展开更多
Cryodamages occur during sperm cryopreservation. Cryopreservation of fish sperm usually results in marked decrease in sperm quality, such as swelling or disruption of the plasma membrane, mi-tochondrial dysfunction, d...Cryodamages occur during sperm cryopreservation. Cryopreservation of fish sperm usually results in marked decrease in sperm quality, such as swelling or disruption of the plasma membrane, mi-tochondrial dysfunction, diminished sperm motility, impaired velocity, shorter motility period, denatura-tion, and release of some enzymes from spermatozoa. In this paper, damages in morphology, physiology, biochemistry and metabolism, and genetic integrity of fish semen after cryopreservation are discussed. New approaches in assessment of fish thawed sperm quality such as computer assisted sperm analysis, flow cytometic analysis combined with fluorescent probes and single cell gel electrophoresis are also briefly reviewed.展开更多
Cryopreservation of Porphyra yezoensis conchocelis was conducted with cryoprotectants and a proposed pretreatment procedure and thawing methods explored. Six cryoprotectants combined by DMSO with ethylene glycol(EG),p...Cryopreservation of Porphyra yezoensis conchocelis was conducted with cryoprotectants and a proposed pretreatment procedure and thawing methods explored. Six cryoprotectants combined by DMSO with ethylene glycol(EG),propylene glycol(PEG),sorbitol and sucrose were developed. The effect of prefreezing at - 40℃ or -20℃ for different time durations was compared and the thawing methods were screened. It was shown that the cryoprotectant including 10% DMSO with 0.5 molL-1 sorbitol exhib-ited the optimal effect. The ideal pretreatment was that conchocelis segments were stayed for 20 min at - 40℃ before stored in liquid nitrogen,and 40℃ water bath was proper for quick thawing. The highest recovery rate of cryopreserved P. yezoensis conchocelis reached 89.41%.展开更多
An underground heat storage system in a double-film-covered greenhouse and an adjacent greenhouse without the heat storage system were designed on the basis of plant physiology to reduce the energy consumption in gree...An underground heat storage system in a double-film-covered greenhouse and an adjacent greenhouse without the heat storage system were designed on the basis of plant physiology to reduce the energy consumption in greenhouses. The results indicated that the floor temperature was respectively 5.2℃, 4.6℃ and 2.0 ℃ higher than that of the soil in the adjacent reference greenhouse after heat storage in a clear, cloudy and overcast sky in winter. Results showed that the temperature and humidity were feasible for plant growth in the heat saving greenhouse.展开更多
基金This research is supported by the NKBRSF (G1999043407-1) Knowledge Innovation Program of the Chinese Academy of Sciences (KZCX2-406 & SCXZD0101).
文摘The fresh pollen vitality, the effect of different storage conditions on the pollen vitality, and the difference of vitality among the species of seven species of Syringa were determined in Shenyang, China. The results indicated that the pollen vi-tality (81.5%) of Syringa villosa was the highest among the seven tested species, followed by S. microphylla and S. meyeri, and that of S. oblata var. affinis was the lowest. The low temperature was the best condition for storage of pollen of Syringa, and the most proper temperature for the storage was 0-2 癈. The storability of S. microphylla was the best of all, and it could be stored over 60 days at the temperature of 0-2 癈, next was S. villosa and S. meyeri.
文摘To explore the change rule of seed vigor and seed application technology of rice PTGMS lines with low temperature storage,Zhun S,Y58S,Feng S and Guangzhan 63-4S stored in the seed bank with the temperature of 8-10 ℃ and with the relative humidity of 55% for different years were used as the materials to study the properties of seed vigor,growth and development in the field, etc. The re- sults showed that the germination potential, germination rate, germination index and vigor index of rice PTGMS lines seeds showed downward trends as the time of low temperature storage prolonged,and all of the germination rates of the seeds within five years were over 60%. The conductivity in the soaked solution of seeds was significantly increased with the prolonged low temperature storage time. Seedling rate,root length,white root number,base stem width and seedling dry weight of seeds showed downward trends as the time of low temperature storage prolonged. There was no significant difference between two integral seedling qualities of seeds within 4-6 years in low temperature storage. Compared with the germination rate of low temperature storage seeds soaking for 6,12 and 24 h,the appropriate soak time of long time low temperature storage or low vigor seeds was 12 h. A serious natural aging phenomenon occurred in low temperature storage seeds after out of the storage. The germination rate of seeds placed under natural condition for 50 d decreased obviously. The duration from seeding to heading of Zhun S,Y58S and Feng S shortened as the time of low temperature storage prolonged, while the vari- ation of panicle duration of all PTGMS lines was not obvious. In conclusion, rice PTGMS line seeds with low temperature storage in 5 years could still be used nor- mally. However.seeds after low temperature storage should be sowed as soon as possible, and the soaking time of low vigor seeds should be shortened,and the possibility that the decreased duration from seeding to heading should also be con- sidered.
基金Supported by Jiangsu Agricultural Science and Technology Innovation Fund[CX(12)1001-04]~~
文摘The greenhouse has been increasingly used in the breeding industry. However, the high temperature inside the greenhouse in summer has not been effectively addressed. The spray cooling system sprays tiny droplets into the air. Thus the water molecules will be vaporized, absorbing heat and reducing ambient temperature. It is the only cooling method that can be used to cool the uneasily-sealed flexible greenhouse. We developed an energy-storing high pressure spray cooling system. The ordinary water pump is used as the source of high-pressure water. The partial kinetic energy is stored in the energy-storing tubes. When the water pump is stopped, the energy produced by releasing the compressed air can still be used to maintain the spray. And thus the use-cost and systematic wear would be reduced. The cooling system only requires 1 kilowatt hour of power per day. It has been widely used in summer to cool the breeding sheds. After a recent continuous improvement, its functions have been extended to disinfection, removing dust, humidifying and immunizing animals. In addition, it can also be used for the cooling and humidifying of squares, venues and streets in summer. The energy-storing high pressure spray cooling system has a broad application prospect.
文摘Aim To study the most stable pH value of endomorphin-1 solution and toestimate the shelf-life of EM-1 solution. Methods The content of EM-1 in its solution was determinedby HPLC, and stability was studied by accelerated tests. Results The most stable pH value of EM-1solution was observed. By the accelerated tests we obtained the Arrhe-nius relationship and thepharmacokinetic parameters. Conclusion The most stable pH value of EM-1 solution is 4.5 -4.8. Thet_(1/2)(25℃) is 45 d.
文摘The effects of temperature, light, pH value and storage time on germina- tion of Adelostemma gracillimum seeds were studied through germination experi- ment. The results showed that A. gracillimum seeds had no property of post-matu- ration dormancy; the optimum germination temperature was 25 ~C; no significant difference was found in germination rate of A. gracillimum seeds between light and dark conditions; A. gracillimum seeds showed a wide adaptability to pH, Le., they could germinate in the pH range of 2-10, and showed a higher germination rate in the pH range of 6-8. The optimum storage time for A. gracillimum seeds was 12 months.
基金Funded by the National High Technology R&D Program of China (863 Program, Nos. 2001AA621100, 2003AA603510 and 2004AA603310).
文摘Cryodamages occur during sperm cryopreservation. Cryopreservation of fish sperm usually results in marked decrease in sperm quality, such as swelling or disruption of the plasma membrane, mi-tochondrial dysfunction, diminished sperm motility, impaired velocity, shorter motility period, denatura-tion, and release of some enzymes from spermatozoa. In this paper, damages in morphology, physiology, biochemistry and metabolism, and genetic integrity of fish semen after cryopreservation are discussed. New approaches in assessment of fish thawed sperm quality such as computer assisted sperm analysis, flow cytometic analysis combined with fluorescent probes and single cell gel electrophoresis are also briefly reviewed.
文摘Cryopreservation of Porphyra yezoensis conchocelis was conducted with cryoprotectants and a proposed pretreatment procedure and thawing methods explored. Six cryoprotectants combined by DMSO with ethylene glycol(EG),propylene glycol(PEG),sorbitol and sucrose were developed. The effect of prefreezing at - 40℃ or -20℃ for different time durations was compared and the thawing methods were screened. It was shown that the cryoprotectant including 10% DMSO with 0.5 molL-1 sorbitol exhib-ited the optimal effect. The ideal pretreatment was that conchocelis segments were stayed for 20 min at - 40℃ before stored in liquid nitrogen,and 40℃ water bath was proper for quick thawing. The highest recovery rate of cryopreserved P. yezoensis conchocelis reached 89.41%.
基金Project (No. 20050912) supported by the Education Department of Zhejiang Province, China
文摘An underground heat storage system in a double-film-covered greenhouse and an adjacent greenhouse without the heat storage system were designed on the basis of plant physiology to reduce the energy consumption in greenhouses. The results indicated that the floor temperature was respectively 5.2℃, 4.6℃ and 2.0 ℃ higher than that of the soil in the adjacent reference greenhouse after heat storage in a clear, cloudy and overcast sky in winter. Results showed that the temperature and humidity were feasible for plant growth in the heat saving greenhouse.
