Objective： Based on a partialsubtilisin-like protease, Prl genomic sequence ofPythium guiyangense which has been cloned before, Panhandle PCR strategy was used to amplify the upstream flanking sequence adjacent to th...Objective： Based on a partialsubtilisin-like protease, Prl genomic sequence ofPythium guiyangense which has been cloned before, Panhandle PCR strategy was used to amplify the upstream flanking sequence adjacent to the known sequence of the Prl gene. Methods： The genomic DNA was firstly digested with BamH I and then treated with calf intestinal alkaline phosphatase（CIAP）. Next, a 5＇ phosphorylated oligonucleotide was ligated to the 5＇ ends of BamH I -digested DNA. After denaturation, intmstrand annealing and polymemse extension, a pan with a handle was formed,and lastly the nested PCR was performed. Results： A 864 bp product was amplified,which was adjacent to the known sequence of Prl gene.The gene has been accessed by GenBank （Accession：JQ975036）. Conclusion： Panhandle PCR is a quick and convenient approach for amplifying and identifying unknown partner genes,which facilitates cloning full-length Prl gene展开更多
The rich biodiversity and cultural patterns of agricultural heritage sites are preserved unconsciously in the traditional way of life of local communities.They are also attractive resources for tourism development,whi...The rich biodiversity and cultural patterns of agricultural heritage sites are preserved unconsciously in the traditional way of life of local communities.They are also attractive resources for tourism development,which supports the conservation of agricultural heritage systems （AHS） while providing benefits for local communities.As a typical case study,a Globally Important Agricultural Heritage System （GIAHS） site with a Rice-Fish-Duck agricultural system （RFDS） in Congjiang County of South West China is introduced to illustrate the community approach to agricultural heritage conservation and sustainable tourism development.The concept of a community approach to agricultural heritage is dynamic both in terms of conservation and livelihood adaptation.Six elements in the concept model and their relationship were analyzed.The community is the core element and the other five are：local community identity,livelihoods,local sustainable tourism development,the farming system,biodiversity and cultural patterns in the site.All are interconnected and thus support the agricultural heritage conservation of agricultural heritage and the development of sustainable tourism in the heritage sites.展开更多
基金Supported by the Guangxi Department of education scientific research funds,China(No.200103YB154)
文摘Objective： Based on a partialsubtilisin-like protease, Prl genomic sequence ofPythium guiyangense which has been cloned before, Panhandle PCR strategy was used to amplify the upstream flanking sequence adjacent to the known sequence of the Prl gene. Methods： The genomic DNA was firstly digested with BamH I and then treated with calf intestinal alkaline phosphatase（CIAP）. Next, a 5＇ phosphorylated oligonucleotide was ligated to the 5＇ ends of BamH I -digested DNA. After denaturation, intmstrand annealing and polymemse extension, a pan with a handle was formed,and lastly the nested PCR was performed. Results： A 864 bp product was amplified,which was adjacent to the known sequence of Prl gene.The gene has been accessed by GenBank （Accession：JQ975036）. Conclusion： Panhandle PCR is a quick and convenient approach for amplifying and identifying unknown partner genes,which facilitates cloning full-length Prl gene
基金China National Natural Science Foundation(Grant No.41201580)the international project of"GEF-FAO/GIAHS dynamic conservation and adaptive management"the Project of Construction of Innovative Teams and Teacher Career Development for Universities and Colleges under Beijing Municipality(IDHT20130513)
文摘The rich biodiversity and cultural patterns of agricultural heritage sites are preserved unconsciously in the traditional way of life of local communities.They are also attractive resources for tourism development,which supports the conservation of agricultural heritage systems （AHS） while providing benefits for local communities.As a typical case study,a Globally Important Agricultural Heritage System （GIAHS） site with a Rice-Fish-Duck agricultural system （RFDS） in Congjiang County of South West China is introduced to illustrate the community approach to agricultural heritage conservation and sustainable tourism development.The concept of a community approach to agricultural heritage is dynamic both in terms of conservation and livelihood adaptation.Six elements in the concept model and their relationship were analyzed.The community is the core element and the other five are：local community identity,livelihoods,local sustainable tourism development,the farming system,biodiversity and cultural patterns in the site.All are interconnected and thus support the agricultural heritage conservation of agricultural heritage and the development of sustainable tourism in the heritage sites.
