AIM:To investigate the expression profile of miRNA in esophageal squamous cell carcinoma(ESCC).METHODS:The expression profile of miRNA in ESCC tissues was analyzed by miRNA microarray.The expression levels of miR-143 ...AIM:To investigate the expression profile of miRNA in esophageal squamous cell carcinoma(ESCC).METHODS:The expression profile of miRNA in ESCC tissues was analyzed by miRNA microarray.The expression levels of miR-143 and miR-145 in 86 ESCC patients were determined by real-time polymerase chain reaction(PCR) using TaqMan assay.The mobility effect was estimated by wound-healing using esophageal carcinoma cells transfected with miRNA expression plasmids.RESULTS:A set of miRNAs was found to be deregulated in the ESCC tissues,and the expression levels of miR-143 and-145 were significantly decreased in most of the ESCC tissues examined.Both miR-143 and miR-145 expression correlated with tumor invasion depth.The transfection of human esophageal carcinoma cells with miR-143 and miR-145 expression plasmids resulted in a greater inhibition of cell mobility,however,the protein level of the previously reported target of miR-145,FSCN1,did not show any significant downregulation.CONCLUSION:These findings suggest that the deregulation of miRNAs plays an important role in the progression of ESCC.Both miR-143 and miR-145 might act as anti-oncomirs common to ESCC.展开更多
Sea level anomalies observed by altimeter during the 1993-2006 period, thermosteric sea level anomalies estimated by using subsurface temperature data produced by Ishii and SODA reanalysis data, tide gauge records and...Sea level anomalies observed by altimeter during the 1993-2006 period, thermosteric sea level anomalies estimated by using subsurface temperature data produced by Ishii and SODA reanalysis data, tide gauge records and HOAPS freshwater flux data were analyzed to investigate the long term sea level change and the water mass balance in the South China Sea, The altime- ter-observed sea level showed a rising rate of （3.5±0.9）mmyr-1 during the period 1993-2006, but this figure was considered to have been highly distorted by the relatively short time interval and the large inter-decadal variability, which apparently exists in both the thermosteric sea level and the observed sea level. Long term thermosteric sea level from 1945 to 2004 gave a rising rate of 0.15±0.06 mmyr-1. Tide gauge＇data revealed this discrepancy and the regional distributions of the sea-level trends. Both the ＇real＇ and the ther- mosteric sea level showed a good correspondence to ENSO： decreasing during El Nino years and increasing during La Nina years. Amplitude and phase differences between the ＇real＇ sea level and the thermosteic sea level were substantially revealed on both sea- sonal and interannual time scales. As one of the possible factors, the freshwater flux might play an important role in balancing the water mass.展开更多
OBJECTIVE: To provide clinical evidence in support of Dahuang Huanglian Xiexin decoction(DHXD) to treat type 2 diabetes mellitus(T2DM) and to introduce a new treatment option for clinicians.METHODS: Retrospective anal...OBJECTIVE: To provide clinical evidence in support of Dahuang Huanglian Xiexin decoction(DHXD) to treat type 2 diabetes mellitus(T2DM) and to introduce a new treatment option for clinicians.METHODS: Retrospective analysis was used to evaluate DHXD for the treatment of T2DM by analyzing clinical records of 183 cases. Patients with T2DM who met the inclusion criteria between January 1,2013 and January 1, 2014 were enrolled. The effects of the treatment were evaluated by the changes in fasting blood-glucose(FBG), postprandial blood sugar(PBG), hemoglobin A1c(Hb Alc), blood lipid profiles and body mass index(BMI) at 1, 2, 3 and 6 months. The changes in main symptoms were alsoevaluated. The dosage of Huanglian(Rhizoma Coptidis) and related factors were analyzed.RESULTS: There was a significant improvement in mean Hb A1C at 3 and 6 months after DHXD treatment compared with the baseline level(P < 0.01).There were also significant improvements in FBG,PBG, blood lipid series and BMI. DHXD also improved the main symptoms of stomach and intestine excessive heat syndrome in patients with obese T2DM. Huanglian(Rhizoma Coptidis) was the most frequently used in 678 clinical visits, the dosage of Huanglian(Rhizoma Coptidis) was related to age, BMI, DM duration, the level of blood glucose,and use of Western hypoglycemic drugs.CONCLUSION: This study suggests that DHXD could decrease blood glucose and improve T2DM symptoms and reduce body weight. The use of DHXD may indicate a new optional treatment for T2 DM.展开更多
基金Supported by Grants from the NSFC-Guangdong Joint Fund,No.U0932001the National Natural Science Foundation of China,No.30900560
文摘AIM:To investigate the expression profile of miRNA in esophageal squamous cell carcinoma(ESCC).METHODS:The expression profile of miRNA in ESCC tissues was analyzed by miRNA microarray.The expression levels of miR-143 and miR-145 in 86 ESCC patients were determined by real-time polymerase chain reaction(PCR) using TaqMan assay.The mobility effect was estimated by wound-healing using esophageal carcinoma cells transfected with miRNA expression plasmids.RESULTS:A set of miRNAs was found to be deregulated in the ESCC tissues,and the expression levels of miR-143 and-145 were significantly decreased in most of the ESCC tissues examined.Both miR-143 and miR-145 expression correlated with tumor invasion depth.The transfection of human esophageal carcinoma cells with miR-143 and miR-145 expression plasmids resulted in a greater inhibition of cell mobility,however,the protein level of the previously reported target of miR-145,FSCN1,did not show any significant downregulation.CONCLUSION:These findings suggest that the deregulation of miRNAs plays an important role in the progression of ESCC.Both miR-143 and miR-145 might act as anti-oncomirs common to ESCC.
基金supported by the National Basic Research Program of China through Grant No. 973-2007CB- 411807
文摘Sea level anomalies observed by altimeter during the 1993-2006 period, thermosteric sea level anomalies estimated by using subsurface temperature data produced by Ishii and SODA reanalysis data, tide gauge records and HOAPS freshwater flux data were analyzed to investigate the long term sea level change and the water mass balance in the South China Sea, The altime- ter-observed sea level showed a rising rate of （3.5±0.9）mmyr-1 during the period 1993-2006, but this figure was considered to have been highly distorted by the relatively short time interval and the large inter-decadal variability, which apparently exists in both the thermosteric sea level and the observed sea level. Long term thermosteric sea level from 1945 to 2004 gave a rising rate of 0.15±0.06 mmyr-1. Tide gauge＇data revealed this discrepancy and the regional distributions of the sea-level trends. Both the ＇real＇ and the ther- mosteric sea level showed a good correspondence to ENSO： decreasing during El Nino years and increasing during La Nina years. Amplitude and phase differences between the ＇real＇ sea level and the thermosteic sea level were substantially revealed on both sea- sonal and interannual time scales. As one of the possible factors, the freshwater flux might play an important role in balancing the water mass.
基金the National Basic Research Program of China(Study of Dose-effect Relationship of Classical Prescription,973 Program,No.2010CB530601)
文摘OBJECTIVE: To provide clinical evidence in support of Dahuang Huanglian Xiexin decoction(DHXD) to treat type 2 diabetes mellitus(T2DM) and to introduce a new treatment option for clinicians.METHODS: Retrospective analysis was used to evaluate DHXD for the treatment of T2DM by analyzing clinical records of 183 cases. Patients with T2DM who met the inclusion criteria between January 1,2013 and January 1, 2014 were enrolled. The effects of the treatment were evaluated by the changes in fasting blood-glucose(FBG), postprandial blood sugar(PBG), hemoglobin A1c(Hb Alc), blood lipid profiles and body mass index(BMI) at 1, 2, 3 and 6 months. The changes in main symptoms were alsoevaluated. The dosage of Huanglian(Rhizoma Coptidis) and related factors were analyzed.RESULTS: There was a significant improvement in mean Hb A1C at 3 and 6 months after DHXD treatment compared with the baseline level(P < 0.01).There were also significant improvements in FBG,PBG, blood lipid series and BMI. DHXD also improved the main symptoms of stomach and intestine excessive heat syndrome in patients with obese T2DM. Huanglian(Rhizoma Coptidis) was the most frequently used in 678 clinical visits, the dosage of Huanglian(Rhizoma Coptidis) was related to age, BMI, DM duration, the level of blood glucose,and use of Western hypoglycemic drugs.CONCLUSION: This study suggests that DHXD could decrease blood glucose and improve T2DM symptoms and reduce body weight. The use of DHXD may indicate a new optional treatment for T2 DM.
