Radio is all about dreams. This old and tired medium now fights with new media as it is with its old enemy television. Radio has a big impact on dreams. It gives you the sound and makes you dream. Once you get used to...Radio is all about dreams. This old and tired medium now fights with new media as it is with its old enemy television. Radio has a big impact on dreams. It gives you the sound and makes you dream. Once you get used to this media you start dreaming. If you used to listen radio more, you get in dependence with it. So this day dreaming change to lifeless dreaming. This article gives an evaluation of how radio audience can shape audience future dreams with dependence theory. The paper starts with a conceptual explanation of dependence theory and goes on with a survey done in Turkey to 101 radio listeners. The survey investigates on how people like listening the radio and what makes them enjoy radio. The survey assumes that people listening to radio and getting depended on it start changing the behaviors and habits of listeners. This behavior change effects listeners future dreams and the audience take radio as a model. The paper shows by using the dependence theory that radio listeners take the radio they love as model and use it to build their future business dreams.展开更多
Severe acute respiratory syndrome coronavirus 2(SARS-Co V-2) relies on the central molecular machine RNA-dependent RNA polymerase(Rd Rp) for the viral replication and transcription. Remdesivir at the template strand h...Severe acute respiratory syndrome coronavirus 2(SARS-Co V-2) relies on the central molecular machine RNA-dependent RNA polymerase(Rd Rp) for the viral replication and transcription. Remdesivir at the template strand has been shown to effectively inhibit the RNA synthesis in SARS-Co V-2 Rd Rp by deactivating not only the complementary UTP incorporation but also the next nucleotide addition. However, the underlying molecular mechanism of the second inhibitory point remains unclear. In this work, we have performed molecular dynamics simulations and demonstrated that such inhibition has not directly acted on the nucleotide addition at the active site. Instead, the translocation of Remdesivir from +1 to-1 site is hindered thermodynamically as the posttranslocation state is less stable than the pre-translocation state due to the motif B residue G683. Moreover, another conserved residue S682 on motif B further hinders the dynamic translocation of Remdesivir due to the steric clash with the 1′-cyano substitution. Overall,our study has unveiled an alternative role of motif B in mediating the translocation when Remdesivir is present in the template strand and complemented our understanding about the inhibitory mechanisms exerted by Remdesivir on the RNA synthesis in SARS-Co V-2 Rd Rp.展开更多
文摘Radio is all about dreams. This old and tired medium now fights with new media as it is with its old enemy television. Radio has a big impact on dreams. It gives you the sound and makes you dream. Once you get used to this media you start dreaming. If you used to listen radio more, you get in dependence with it. So this day dreaming change to lifeless dreaming. This article gives an evaluation of how radio audience can shape audience future dreams with dependence theory. The paper starts with a conceptual explanation of dependence theory and goes on with a survey done in Turkey to 101 radio listeners. The survey investigates on how people like listening the radio and what makes them enjoy radio. The survey assumes that people listening to radio and getting depended on it start changing the behaviors and habits of listeners. This behavior change effects listeners future dreams and the audience take radio as a model. The paper shows by using the dependence theory that radio listeners take the radio they love as model and use it to build their future business dreams.
基金supported by the National Key RD program of China(No.2021YFA1502300)the National Natural Science Foundation of China(No.21733007)。
文摘Severe acute respiratory syndrome coronavirus 2(SARS-Co V-2) relies on the central molecular machine RNA-dependent RNA polymerase(Rd Rp) for the viral replication and transcription. Remdesivir at the template strand has been shown to effectively inhibit the RNA synthesis in SARS-Co V-2 Rd Rp by deactivating not only the complementary UTP incorporation but also the next nucleotide addition. However, the underlying molecular mechanism of the second inhibitory point remains unclear. In this work, we have performed molecular dynamics simulations and demonstrated that such inhibition has not directly acted on the nucleotide addition at the active site. Instead, the translocation of Remdesivir from +1 to-1 site is hindered thermodynamically as the posttranslocation state is less stable than the pre-translocation state due to the motif B residue G683. Moreover, another conserved residue S682 on motif B further hinders the dynamic translocation of Remdesivir due to the steric clash with the 1′-cyano substitution. Overall,our study has unveiled an alternative role of motif B in mediating the translocation when Remdesivir is present in the template strand and complemented our understanding about the inhibitory mechanisms exerted by Remdesivir on the RNA synthesis in SARS-Co V-2 Rd Rp.
