The cooperation effects of GA3, IAA and uniconazole-P were studied on the gravitropism and wood formation in Fraxinus mandshurica Rupr. var.japonica Maxim. Seedlings using traditional paraffin section technology. Our ...The cooperation effects of GA3, IAA and uniconazole-P were studied on the gravitropism and wood formation in Fraxinus mandshurica Rupr. var.japonica Maxim. Seedlings using traditional paraffin section technology. Our results are： （1） Gravitropism of stems was strongly inhibited only in B, whereas promoted significantly in D, E, F, H and I treatments; （2） Xylem formation was increased on both sides in H, I and J treatments and on the lower side in E and F which also show the synergistic effect; （3） On the radial direction, cell wall thickness was enhanced on the upper side, whereas decreased on the lower side in C, and was also significantly promoted on the upper in E, G, H, I and J treatments. On the tangential direction, cell wall thickness was increased on the lower side in E, H, I and J treatments respectively; （4） Gelatinous layer of wood fibers was observed on the upper side in all treatments. These results suggest that both GA3 and IAA affected negative gravitropism and wood formation significantly. However, appliedor unapplited-uniconazole-P does not affect G-layer formation, indicating GA does not play the key role on G-layer formation, and ratio of GA3/IAA or IAA may be more important in regulating G-layer formation.展开更多
A one-step dual flow immunochromatographic assay （DICGA）, based on a competitive format, was de- veloped for simultaneous quantification of ochratoxin A （OTA） and zearalenone （ZEN） in corn, wheat, and feed sam- ...A one-step dual flow immunochromatographic assay （DICGA）, based on a competitive format, was de- veloped for simultaneous quantification of ochratoxin A （OTA） and zearalenone （ZEN） in corn, wheat, and feed sam- ples. The limit of detection for OTA was 0.32 ng/ml with a detection range of 0.53-12.16 ng/ml, while for ZEN it was 0.58 ng/ml with a detection range of 1.06-39.72 ng/ml. The recovery rates in corn, wheat, and feed samples ranged from 77.3% to 106.3% with the coefficient of variation lower than 15%. Naturally contaminated corn, wheat, and feed samples were analyzed using both DICGA and liquid chromatography-tandem mass spectrometry （LC-MS/MS） and the correlation between the two methods was evaluated using a regression analysis. The DICGA method shows great potential for simple, rapid, sensitive, and cost-effective quantitative detection of OTA and ZEN in food safety control.展开更多
基金This research was supported by the scholarship from the Japanese Ministry of Education （No. 07456073）, Scientific Research Foundation for the Returned Oversea Chinese Scholars, State Education Ministry of China, Natural Science Foundation of Tianjin, China （No. 07JCYBJCI2400 and No. 07JCYBJCI2500） and National Key Basic Research Plan Proiect （No. 2007CB 106802）.
文摘The cooperation effects of GA3, IAA and uniconazole-P were studied on the gravitropism and wood formation in Fraxinus mandshurica Rupr. var.japonica Maxim. Seedlings using traditional paraffin section technology. Our results are： （1） Gravitropism of stems was strongly inhibited only in B, whereas promoted significantly in D, E, F, H and I treatments; （2） Xylem formation was increased on both sides in H, I and J treatments and on the lower side in E and F which also show the synergistic effect; （3） On the radial direction, cell wall thickness was enhanced on the upper side, whereas decreased on the lower side in C, and was also significantly promoted on the upper in E, G, H, I and J treatments. On the tangential direction, cell wall thickness was increased on the lower side in E, H, I and J treatments respectively; （4） Gelatinous layer of wood fibers was observed on the upper side in all treatments. These results suggest that both GA3 and IAA affected negative gravitropism and wood formation significantly. However, appliedor unapplited-uniconazole-P does not affect G-layer formation, indicating GA does not play the key role on G-layer formation, and ratio of GA3/IAA or IAA may be more important in regulating G-layer formation.
基金Project supported by the Natural Science Foundation of Zhejiang Province(No.LQ17C170002)the Talent-Start Project of Zhejiang A&F University(No.2016FR025)+1 种基金the Key Research and Development Project Funds of Zhejiang Provincial Science and Technology Department(No.2018C02041)and the National High-Tech R&D Program(863)of China(No.2012AA101602)
文摘A one-step dual flow immunochromatographic assay （DICGA）, based on a competitive format, was de- veloped for simultaneous quantification of ochratoxin A （OTA） and zearalenone （ZEN） in corn, wheat, and feed sam- ples. The limit of detection for OTA was 0.32 ng/ml with a detection range of 0.53-12.16 ng/ml, while for ZEN it was 0.58 ng/ml with a detection range of 1.06-39.72 ng/ml. The recovery rates in corn, wheat, and feed samples ranged from 77.3% to 106.3% with the coefficient of variation lower than 15%. Naturally contaminated corn, wheat, and feed samples were analyzed using both DICGA and liquid chromatography-tandem mass spectrometry （LC-MS/MS） and the correlation between the two methods was evaluated using a regression analysis. The DICGA method shows great potential for simple, rapid, sensitive, and cost-effective quantitative detection of OTA and ZEN in food safety control.
